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The map label for this gene is narI [H]

Identifier: 94311015

GI number: 94311015

Start: 2256415

End: 2257098

Strand: Direct

Name: narI [H]

Synonym: Rmet_2077

Alternate gene names: 94311015

Gene position: 2256415-2257098 (Clockwise)

Preceding gene: 94311014

Following gene: 94311016

Centisome position: 57.44

GC content: 63.74

Gene sequence:

>684_bases
ATGAACCTGGTCCATCAATTTATCTTCGGGATCTATCCCTACATCGCAGCCGCCATCTTCCTGTTCGGCAGCCTGGCGCG
CTTCGAGCGCGAGCAGTACACCTGGAAGTCGGACAGTTCGCAGATGCTCTATCGCGGTAACCTGCGCACCGGCAACATCC
TGTTCCACGTGGGCATCCTTGGCCTGTTCTTCGGCCATCTCGTCGGCCTGCTGACGCCGGTCGTGGTGTGGGACACGCTT
GGCGTGTCGCATGGCCTCAAGCAGGCCATCGCCATGAGCGCCGGCGGCGTCATGGGCACGATGTGCCTGGCCGGCCTGCT
GATCCTGCTGCATCGTCGCCTGACCAACGCACGCGTGTCCGCCGTCACGCGCTTCGGTGACAAGGTCCTGCTGCTGTGGC
TGCTGGTCACGCTGCTACTGGGTCTGTCGACGATCTTCGAGTCGGCCAGCCATATGGACGGCCACATGATGGTGCAACTG
ATGACCTGGGCGCAGCACGTCGTCACGCTGCGCGCGGATGCCGCCAGCTATGTGGCCGAGGCCCCGCTGCTCTTCAAGGC
GCACCTGTTCATGGGCATCTCGCTGTTCGCGATCTTCCCGTTCACGCGACTGGTGCACGTCTGGAGCGGCTTTGCCTCGG
TGACCTACCTGGGACGCGCCTGGCAACTCGTCCGGGGCCGGTAA

Upstream 100 bases:

>100_bases
GTGAAACCAGCCGTCGACGTGATCACGTTCCACAAGCGCGCCGCGCCGTCCCCTCGCCCGTGAGCCCGTCCATTACAAGG
AACCAACCAGAGCCACTACC

Downstream 100 bases:

>100_bases
GGCCGTGTCCCCATGCACGAGATCGAACGTCTGCTCAAGGGCTTCGAACGGTTCCAGCGGCGCTACTTCGAGGCCAGGCC
GGAACTCTTCGATGCCCTGC

Product: nitrate reductase 1, gamma (cytochrome b(NR)) subunit

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MNLVHQFIFGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQMLYRGNLRTGNILFHVGILGLFFGHLVGLLTPVVVWDTL
GVSHGLKQAIAMSAGGVMGTMCLAGLLILLHRRLTNARVSAVTRFGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQL
MTWAQHVVTLRADAASYVAEAPLLFKAHLFMGISLFAIFPFTRLVHVWSGFASVTYLGRAWQLVRGR

Sequences:

>Translated_227_residues
MNLVHQFIFGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQMLYRGNLRTGNILFHVGILGLFFGHLVGLLTPVVVWDTL
GVSHGLKQAIAMSAGGVMGTMCLAGLLILLHRRLTNARVSAVTRFGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQL
MTWAQHVVTLRADAASYVAEAPLLFKAHLFMGISLFAIFPFTRLVHVWSGFASVTYLGRAWQLVRGR
>Mature_227_residues
MNLVHQFIFGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQMLYRGNLRTGNILFHVGILGLFFGHLVGLLTPVVVWDTL
GVSHGLKQAIAMSAGGVMGTMCLAGLLILLHRRLTNARVSAVTRFGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQL
MTWAQHVVTLRADAASYVAEAPLLFKAHLFMGISLFAIFPFTRLVHVWSGFASVTYLGRAWQLVRGR

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=227, Percent_Identity=46.2555066079295, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI1787738, Length=227, Percent_Identity=44.0528634361233, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25271; Mature: 25271

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVHQFIFGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQMLYRGNLRTGNILFHVGIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
GLFFGHLVGLLTPVVVWDTLGVSHGLKQAIAMSAGGVMGTMCLAGLLILLHRRLTNARVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AVTRFGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQLMTWAQHVVTLRADAASYVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
APLLFKAHLFMGISLFAIFPFTRLVHVWSGFASVTYLGRAWQLVRGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLVHQFIFGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQMLYRGNLRTGNILFHVGIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
GLFFGHLVGLLTPVVVWDTLGVSHGLKQAIAMSAGGVMGTMCLAGLLILLHRRLTNARVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AVTRFGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQLMTWAQHVVTLRADAASYVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
APLLFKAHLFMGISLFAIFPFTRLVHVWSGFASVTYLGRAWQLVRGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]