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The map label for this gene is glnL [H]

Identifier: 94310998

GI number: 94310998

Start: 2232479

End: 2233642

Strand: Reverse

Name: glnL [H]

Synonym: Rmet_2060

Alternate gene names: 94310998

Gene position: 2233642-2232479 (Counterclockwise)

Preceding gene: 94310999

Following gene: 94310997

Centisome position: 56.86

GC content: 63.92

Gene sequence:

>1164_bases
ATGCGCCGCCTGATTCGTGAAGTGTCCCGCAAGGTAGCAGGAACGGACCGCAAGCCGGACGCCGGCGAGCCGGCCGATCG
TGATGCGGACATCCTGCCGCTGGGCGGCGCGGCCCTGCATCCGGGCCTGGATGTAGTGGCCAACCCTGTGCTGCTGGTGC
GCCAGCCGGGCCTGCGGGTGATTTACGCGAATCCGGCGGCGGAGGCCAGCTTTGCCGTGTCGCGCAAGGCCATGGTGGAA
CTGTCCCTGCCGGACCTGTTCGGGGCGTCGGAAGAACTCGACAGCATGTTCGAGACGGTGATGGCCCGGCAGGTGGACGT
GCGCAGGCAGGATCTGGTCCTGCGGCCGCCATTGCAGGAACCGATGCACGCGCACGTGGTAATCGGTGGCGTGGAAGGCT
ACGCGAACACAGCGGTGGTCGAGGTGCTGCTCAACGAGCAGAAGGTGCGCAGTGACCGCGAGGAGCGCATTCTTGACCTG
ACCTCGGCGAACAAGGAACTGATCCGCAATCTGGCTCACGAGATCAAGAACCCGCTGGGCGGGATTCGCGGCGCGGCCCA
GTTGCTCGAATTCGAGCTGCCCGAGCGTGCACTCCGGGAGTACACGCAGGTCATCATCAAGGAGTCGGACCGCCTGCAAA
CGCTGGTGGACCGCCTGCTGGAGCCGCATCGCCATCCGCACATCGTCTCGGACCTCAATATCCACGAGGTGCTGGAGCGT
GTGCGCTCGGTGGTGCTGGCCGAGTTTCCCAACGGGCTGGAAATCGTGCGCGACTACGATGCCAGCTTGCCAGAATTGCG
CGGCGATATGGAGCAACTGATCCAGGCCGTGCTGAACATCGTCCATAACGCCGCCCAGGCGCTGCACGAGCGTGTTGCGC
GCGGCGATGCGCAGATCATCCTGCGGACCCGGGTGGCCCGGCAGGTCACGATCGCCAAGCGGCTCTTCAAGCTGGCATTG
GATTTGCATGTGATTGACAACGGCCCGGGAATCCCCGAGGACATTCGCGAGCGCATCTTCTATCCGTTGGTTTCGGGCAG
GGATGGCGGTAGCGGGCTCGGTCTCACACTCGCTCAAACCTTCGTGCAGCAACACGAGGGCTTGATCGAATGCGAGAGCA
AGCCGGGCTGTACCGACTTCCGTATCCTGTTGCCATTGCACTAA

Upstream 100 bases:

>100_bases
ACCTGGATCGCGTCGCGCGGCTTCGTGACGACATCACCCGCAGCGAAGCCAATGTGGCATCGTTGCGCCGGGAGCTTTCC
GGCTTGAAGGATTGAGTTGA

Downstream 100 bases:

>100_bases
CCGCCCGATCGGCGAGACGGTGCGAGCGGCGCGGGGCCCTGCCATCAGCAGGGAATACGGGAGACCACATGACACCGAGC
AGACAAGCGACGCACATGAA

Product: NtrB, signal transduction histidine kinase; nitrogen specific

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MRRLIREVSRKVAGTDRKPDAGEPADRDADILPLGGAALHPGLDVVANPVLLVRQPGLRVIYANPAAEASFAVSRKAMVE
LSLPDLFGASEELDSMFETVMARQVDVRRQDLVLRPPLQEPMHAHVVIGGVEGYANTAVVEVLLNEQKVRSDREERILDL
TSANKELIRNLAHEIKNPLGGIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSDLNIHEVLER
VRSVVLAEFPNGLEIVRDYDASLPELRGDMEQLIQAVLNIVHNAAQALHERVARGDAQIILRTRVARQVTIAKRLFKLAL
DLHVIDNGPGIPEDIRERIFYPLVSGRDGGSGLGLTLAQTFVQQHEGLIECESKPGCTDFRILLPLH

Sequences:

>Translated_387_residues
MRRLIREVSRKVAGTDRKPDAGEPADRDADILPLGGAALHPGLDVVANPVLLVRQPGLRVIYANPAAEASFAVSRKAMVE
LSLPDLFGASEELDSMFETVMARQVDVRRQDLVLRPPLQEPMHAHVVIGGVEGYANTAVVEVLLNEQKVRSDREERILDL
TSANKELIRNLAHEIKNPLGGIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSDLNIHEVLER
VRSVVLAEFPNGLEIVRDYDASLPELRGDMEQLIQAVLNIVHNAAQALHERVARGDAQIILRTRVARQVTIAKRLFKLAL
DLHVIDNGPGIPEDIRERIFYPLVSGRDGGSGLGLTLAQTFVQQHEGLIECESKPGCTDFRILLPLH
>Mature_387_residues
MRRLIREVSRKVAGTDRKPDAGEPADRDADILPLGGAALHPGLDVVANPVLLVRQPGLRVIYANPAAEASFAVSRKAMVE
LSLPDLFGASEELDSMFETVMARQVDVRRQDLVLRPPLQEPMHAHVVIGGVEGYANTAVVEVLLNEQKVRSDREERILDL
TSANKELIRNLAHEIKNPLGGIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSDLNIHEVLER
VRSVVLAEFPNGLEIVRDYDASLPELRGDMEQLIQAVLNIVHNAAQALHERVARGDAQIILRTRVARQVTIAKRLFKLAL
DLHVIDNGPGIPEDIRERIFYPLVSGRDGGSGLGLTLAQTFVQQHEGLIECESKPGCTDFRILLPLH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=347, Percent_Identity=41.2103746397695, Blast_Score=227, Evalue=8e-61,
Organism=Escherichia coli, GI1788549, Length=245, Percent_Identity=30.6122448979592, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790436, Length=244, Percent_Identity=29.9180327868852, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790346, Length=240, Percent_Identity=26.25, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43076; Mature: 43076

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLIREVSRKVAGTDRKPDAGEPADRDADILPLGGAALHPGLDVVANPVLLVRQPGLRV
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHCCCHHHHHCCEEEEECCCCEE
IYANPAAEASFAVSRKAMVELSLPDLFGASEELDSMFETVMARQVDVRRQDLVLRPPLQE
EEECCCCCHHHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCCC
PMHAHVVIGGVEGYANTAVVEVLLNEQKVRSDREERILDLTSANKELIRNLAHEIKNPLG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSDLNIHEVLER
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
VRSVVLAEFPNGLEIVRDYDASLPELRGDMEQLIQAVLNIVHNAAQALHERVARGDAQII
HHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LRTRVARQVTIAKRLFKLALDLHVIDNGPGIPEDIRERIFYPLVSGRDGGSGLGLTLAQT
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
FVQQHEGLIECESKPGCTDFRILLPLH
HHHHCCCCEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MRRLIREVSRKVAGTDRKPDAGEPADRDADILPLGGAALHPGLDVVANPVLLVRQPGLRV
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHCCCHHHHHCCEEEEECCCCEE
IYANPAAEASFAVSRKAMVELSLPDLFGASEELDSMFETVMARQVDVRRQDLVLRPPLQE
EEECCCCCHHHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCCC
PMHAHVVIGGVEGYANTAVVEVLLNEQKVRSDREERILDLTSANKELIRNLAHEIKNPLG
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GIRGAAQLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVSDLNIHEVLER
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
VRSVVLAEFPNGLEIVRDYDASLPELRGDMEQLIQAVLNIVHNAAQALHERVARGDAQII
HHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LRTRVARQVTIAKRLFKLALDLHVIDNGPGIPEDIRERIFYPLVSGRDGGSGLGLTLAQT
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
FVQQHEGLIECESKPGCTDFRILLPLH
HHHHCCCCEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]