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The map label for this gene is glnG [H]

Identifier: 94310997

GI number: 94310997

Start: 2230824

End: 2232410

Strand: Reverse

Name: glnG [H]

Synonym: Rmet_2059

Alternate gene names: 94310997

Gene position: 2232410-2230824 (Counterclockwise)

Preceding gene: 94310998

Following gene: 94310993

Centisome position: 56.83

GC content: 63.39

Gene sequence:

>1587_bases
ATGACACCGAGCAGACAAGCGACGCACATGAAGCCGATCTGGATAGTCGACGACGATCAATCAATCCGCTGGGTCCTGGA
AAAGGCCCTTGCCCGTGAAAGCCTGCTCTCCCGCAGTTTTACCAACGTGCGCGACGTGCTCGCGGCGCTGGAAGAGGACG
AGCCGCAGGTCCTGATTTCGGATATCCGAATGCCCGGCGGTTCCGGCCTGGATTTGCTGCAGGCCATCAAGGCGCGGCAT
CCGGGCCTGCCCGTTATCGTGATGACGGCCTACTCGGACCTGGATAGCGCCGTGGCCGCGTTCCAGGGGGGCGCGTTCGA
GTACCTGGCCAAGCCGTTCGATGTCGACAAGGCGGTCGAGTTGATTCGCCGGGCGCTCGAGGAAAGCCTGCGCGAGGAGG
AAATGGACGATCGCCTGGTCGATGCGCCGGAAATCCTCGGCCAGGCTCCGGCGATGCAGGACGTGTTCCGCGCGATCGGT
CGCCTCTCGCAATCGAACGTCACCGTAATGATCACCGGCGAGTCCGGTACCGGCAAGGAACTGGTGGCGCGTGCGTTGCA
CAAGCACAGCCCGCGCGCAAATGGTCCGTTCATTGCGCTGAATACGGCGGCAATTCCCAAGGATCTGCTCGAATCGGAAC
TGTTCGGCCATGAGCGTGGCGCGTTCACCGGCGCGCAGACCATGCGTCGCGGTCGCTTCGAACAGGCCGAAGGTGGCACG
CTGTTCCTCGACGAAATCGGCGATATGCCATTTGATTTGCAGACGCGCCTGCTGCGTGTACTTTCGGATGGAAATTTCTA
TCGGGTGGGGGGGCATAATCCGTTGCGCGCGAACGTGCGCGTCATCGCCGCCACGCACCAGAACCTCGAGACGCGCGTCA
AGGAAGGGCTGTTCCGCGAGGATTTGTTCCACCGTCTCAACGTGATTCGCCTGCGCCTGCCGCCGCTGCGCGAGCGTCCG
GAGGACATCACGCTGCTGGCCCGCCATTTCCTGCAGAAGAGCGCGAAGGAGCTTGGCGTCGAAACCAAGCGCATGTCCGA
TGACGCACTGGCCTACGTCAGCACGCTGCCGTTCCCCGGCAATGTGCGCCAGCTCGAGAACCTCTGCAACTGGCTGACCG
TCATGGCGCCGGCGCAGACCATCGAAGTAAAGGATTTCCCGCGCGAAATGATCGAGGTGGGGTACGGCGAAAGCAGTGTG
TCGCCGATGCGTTCGCCGGGCGAGTCGAGGTCGTCGGGAGAGTACGAGGCTGCGGAAGTTTCTGACTACGGCACGCATGC
CACGGGCGAGGGCGACACGATGGTCGCCACAAGACCACCCATGGCCTCTTCGGCTACGTCGTCGGCGTCGGCATTTGCGG
CGAGCGCCGTTTCGGCAAGCTGGGAAACGCTGCTGGCTGGCGAGGCAAAGTCGATGCTCGAATCGGGGCAGCCCGAAGTC
ATGGATGCGCTGACGCGGCGATTCGAGAAAGCGGTGCTGGAAGCCGCGCTCGGTGTCACGCGCGGACGTCGCGTGGAGGC
CGCGACGCGGCTCGGCATCGGTCGGAACACCATTACTCGCAAGCTGCAGGAGCTTGGGTTCGACTGA

Upstream 100 bases:

>100_bases
CCGACTTCCGTATCCTGTTGCCATTGCACTAACCGCCCGATCGGCGAGACGGTGCGAGCGGCGCGGGGCCCTGCCATCAG
CAGGGAATACGGGAGACCAC

Downstream 100 bases:

>100_bases
TCGCAGACTCGTAGGTTTGTGGACCTGGATCGAGAGGCCTCGCGCTCGCGGGGCCTCTCTTATTTGATGGTTTCCACTCA
GGGACTAACCGGCTCTGGCA

Product: NtrC, nitrogen regulation protein NR(I); transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MTPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDVLAALEEDEPQVLISDIRMPGGSGLDLLQAIKARH
PGLPVIVMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIG
RLSQSNVTVMITGESGTGKELVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGT
LFLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLETRVKEGLFREDLFHRLNVIRLRLPPLRERP
EDITLLARHFLQKSAKELGVETKRMSDDALAYVSTLPFPGNVRQLENLCNWLTVMAPAQTIEVKDFPREMIEVGYGESSV
SPMRSPGESRSSGEYEAAEVSDYGTHATGEGDTMVATRPPMASSATSSASAFAASAVSASWETLLAGEAKSMLESGQPEV
MDALTRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD

Sequences:

>Translated_528_residues
MTPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDVLAALEEDEPQVLISDIRMPGGSGLDLLQAIKARH
PGLPVIVMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIG
RLSQSNVTVMITGESGTGKELVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGT
LFLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLETRVKEGLFREDLFHRLNVIRLRLPPLRERP
EDITLLARHFLQKSAKELGVETKRMSDDALAYVSTLPFPGNVRQLENLCNWLTVMAPAQTIEVKDFPREMIEVGYGESSV
SPMRSPGESRSSGEYEAAEVSDYGTHATGEGDTMVATRPPMASSATSSASAFAASAVSASWETLLAGEAKSMLESGQPEV
MDALTRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD
>Mature_527_residues
TPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDVLAALEEDEPQVLISDIRMPGGSGLDLLQAIKARHP
GLPVIVMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIGR
LSQSNVTVMITGESGTGKELVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTL
FLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLETRVKEGLFREDLFHRLNVIRLRLPPLRERPE
DITLLARHFLQKSAKELGVETKRMSDDALAYVSTLPFPGNVRQLENLCNWLTVMAPAQTIEVKDFPREMIEVGYGESSVS
PMRSPGESRSSGEYEAAEVSDYGTHATGEGDTMVATRPPMASSATSSASAFAASAVSASWETLLAGEAKSMLESGQPEVM
DALTRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=518, Percent_Identity=54.6332046332046, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1788550, Length=404, Percent_Identity=44.3069306930693, Blast_Score=346, Evalue=3e-96,
Organism=Escherichia coli, GI1790437, Length=378, Percent_Identity=42.3280423280423, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1788905, Length=335, Percent_Identity=45.3731343283582, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI87082117, Length=248, Percent_Identity=50.8064516129032, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1789087, Length=225, Percent_Identity=51.5555555555556, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1789233, Length=264, Percent_Identity=46.5909090909091, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI87082152, Length=239, Percent_Identity=47.6987447698745, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1786524, Length=245, Percent_Identity=48.1632653061224, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1787583, Length=224, Percent_Identity=45.9821428571429, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=47.1615720524017, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=31.5789473684211, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081858, Length=229, Percent_Identity=32.7510917030568, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790860, Length=114, Percent_Identity=32.4561403508772, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58081; Mature: 57950

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50330 UIM ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDVLAALEEDEPQVLIS
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
DIRMPGGSGLDLLQAIKARHPGLPVIVMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVE
HHCCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
LIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIGRLSQSNVTVMITGESGTGKE
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHH
LVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGT
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCE
LFLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLETRVKEGLFRE
EEHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHH
DLFHRLNVIRLRLPPLRERPEDITLLARHFLQKSAKELGVETKRMSDDALAYVSTLPFPG
HHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCCC
NVRQLENLCNWLTVMAPAQTIEVKDFPREMIEVGYGESSVSPMRSPGESRSSGEYEAAEV
CHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
SDYGTHATGEGDTMVATRPPMASSATSSASAFAASAVSASWETLLAGEAKSMLESGQPEV
HCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MDALTRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPSRQATHMKPIWIVDDDQSIRWVLEKALARESLLSRSFTNVRDVLAALEEDEPQVLIS
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
DIRMPGGSGLDLLQAIKARHPGLPVIVMTAYSDLDSAVAAFQGGAFEYLAKPFDVDKAVE
HHCCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCHHHHHH
LIRRALEESLREEEMDDRLVDAPEILGQAPAMQDVFRAIGRLSQSNVTVMITGESGTGKE
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHH
LVARALHKHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGT
HHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCE
LFLDEIGDMPFDLQTRLLRVLSDGNFYRVGGHNPLRANVRVIAATHQNLETRVKEGLFRE
EEHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHH
DLFHRLNVIRLRLPPLRERPEDITLLARHFLQKSAKELGVETKRMSDDALAYVSTLPFPG
HHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCCC
NVRQLENLCNWLTVMAPAQTIEVKDFPREMIEVGYGESSVSPMRSPGESRSSGEYEAAEV
CHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
SDYGTHATGEGDTMVATRPPMASSATSSASAFAASAVSASWETLLAGEAKSMLESGQPEV
HCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MDALTRRFEKAVLEAALGVTRGRRVEAATRLGIGRNTITRKLQELGFD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]