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The map label for this gene is rluB [H]

Identifier: 94310972

GI number: 94310972

Start: 2201993

End: 2203888

Strand: Reverse

Name: rluB [H]

Synonym: Rmet_2034

Alternate gene names: 94310972

Gene position: 2203888-2201993 (Counterclockwise)

Preceding gene: 94310973

Following gene: 94310971

Centisome position: 56.11

GC content: 67.51

Gene sequence:

>1896_bases
TTGTCTGATTCCGCTGAGCAAGACGTCCGCCAGCCGGACGTGCCGGGCGATACCGGCGCAGCCCCCGAGGGTAACGGCGC
CGAAGGCGCGCCCCGCCGCAAGGGGCTGCGCCGCGGCCTGCGCAATCTAGTGGCTTCCCGTCGCCAGGCGGGGCAGGACC
GCGATGGCCGGGAAACGGGGCGCGACGATGTCGAACCCTCGTCGACTGGCGAAAGCGGCGATGCTTCGCCGGCCGCCGCC
CCCGCGCGTGGCCGTGGGCGTCGCAAGCCGGCGCCGGCCGCCGACACGCAGGCAGTTGCTGGTGCCGAGGAGATGGGTGG
CGACGCGCAAGCTGGCCAGCCGCGATCGGATCAGCCAGCGCAGGGGGCTCGCAAGCGCCGTCAGCAGCCGCAACGCAAGG
CAGCGCTGGTTGATGGTGACAACAAGCCAACCTCACAACAGCCGGGCCGCCGCAACAAGGGTGGTCAGCGCAAGGGTCAA
CAGCCTGGCGGCAAGCCGGGTGCTGACAGGGGTGACCAGCGTGTCGAGCAGCGCCAGGGCGACAAGCCCGGTCGGCAAGC
CGGCAAGGGCGGCGCGAAGACCGCTGCCGGTGGCGAGGATCTGTTCCGTTTCGTGATTTCCGAGCAGTTCGATCAGGAAG
ACACGATTGTGCCGCGCACCAAGGCCAAGCCGGTGCGCGAACTGTCCGCAGACGACGACGCGCCCAAACTGCACAAGGTG
CTGGCGGAGGGTGGACTTGGTTCACGCCGCGAGATGGAAGAACTGATCCTGCAGGGGCGTGTGTCGGTCAATGGCCTGCC
TGCCCATATCGGCCAGCGTATTCTGCCGACCGACCAGGTCCGCGTGAACGGCAAGCTGATTCACCGCAAGGTGACGACCA
AGCCTCCACGGGTGCTGCTCTATCACAAGCCCTCCGGCGAGATCGTCAGCCAGTCGGATCCGGAAGGCCGTCCGACCGTT
TTCGACAGTCTCCCGCGCATCAAGAACGGCAAGTGGGTCGCGGTTGGTCGCCTCGACTTCAATACCGAAGGGCTGCTGAT
TTTCACCACGTCGGGTGATATCGCGAACCGTTTCATGCACCCGCGCTATGGCGTGGAGCGCGAGTACGCAGTGCGCACGC
TGGGCGAACTGGCCGAAGCCGATCGTCAGCGGCTGCTGCATGGCATTCAGCTCGATGATGGCGAGGCAAACTTCCTGCGG
ATCGCCGATGGCGGTGGCGAGGGCGTGAACCAGTGGTATCACGTGGCGCTGACCGAAGGCCGTAACCGCGAAGTGCGTCG
CATGTTCGAGGCCGTCGGTCTGACCGTGTCGCGCCTGATCCGCACGCGTTATGGTCAGTTCCTGCTGCCGCGCGGCTTGA
AGCGCGGTCGCTGGCAGGAATTGGACCCGACGGATGTGCGCGGCCTGATGGCCAACATTGGACTGAAGGCGCCGTCCAAG
GAAGCACAGAGTGGCGGCAAGGGTGTGACGAAGGTCGGCGGCCGCAAGCAGCGCGAAGAAGCGGCGCTGGCGGGCATGCC
GATGCACACCGGCATGGATGGTCTGCCGCGCTACGAGAAGAGCGGTGGCCGGGGTGGTAATGCTGGGGGCGCGGGCCGTG
GTCAGCCTGATCCGATGCAGACCTCGATGGGATACATTCCGTCCGGCCCTGCGCCGCTGACGTCGCATACGACCAAGTTT
GGCGGCGGGATGGGCGGCGGCCTGCGGGGCCCTGGCGCCGGTGGTGGTCGCGGCGGCGGGCGGGGCGGCAAGGCCGGAAA
CGCTGGCAATGCGGGCAACGCAGGTGGCAATCGCCAGCGTCGCGGCGGTGGCGAGGTCAATGGAAATGTAATGCCAAAGG
CTGCCAAGGGTGCGGGAGGCGGTAGTCGCCGACCGCGCGGCCGAGGTCAGCGTTGA

Upstream 100 bases:

>100_bases
GCCGCAGTTCCGGCTCGCGACGGTGATATGCCGCTGACGACCGAGGACGACGACGAGCGCGTGTCCAACTGAACAACATG
CAACATCGATAGTGAATACT

Downstream 100 bases:

>100_bases
TTGACGGATCGGGCGGTGCCGGTAATGGTCGGCAATCGCCCCGATCCGGAGCGGAACCTCGGAGGCTTTTGCCTTTTGGA
CATAATCCGCGTATAATCAA

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSDSAEQDVRQPDVPGDTGAAPEGNGAEGAPRRKGLRRGLRNLVASRRQAGQDRDGRETGRDDVEPSSTGESGDASPAAA
PARGRGRRKPAPAADTQAVAGAEEMGGDAQAGQPRSDQPAQGARKRRQQPQRKAALVDGDNKPTSQQPGRRNKGGQRKGQ
QPGGKPGADRGDQRVEQRQGDKPGRQAGKGGAKTAAGGEDLFRFVISEQFDQEDTIVPRTKAKPVRELSADDDAPKLHKV
LAEGGLGSRREMEELILQGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVTTKPPRVLLYHKPSGEIVSQSDPEGRPTV
FDSLPRIKNGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAEADRQRLLHGIQLDDGEANFLR
IADGGGEGVNQWYHVALTEGRNREVRRMFEAVGLTVSRLIRTRYGQFLLPRGLKRGRWQELDPTDVRGLMANIGLKAPSK
EAQSGGKGVTKVGGRKQREEAALAGMPMHTGMDGLPRYEKSGGRGGNAGGAGRGQPDPMQTSMGYIPSGPAPLTSHTTKF
GGGMGGGLRGPGAGGGRGGGRGGKAGNAGNAGNAGGNRQRRGGGEVNGNVMPKAAKGAGGGSRRPRGRGQR

Sequences:

>Translated_631_residues
MSDSAEQDVRQPDVPGDTGAAPEGNGAEGAPRRKGLRRGLRNLVASRRQAGQDRDGRETGRDDVEPSSTGESGDASPAAA
PARGRGRRKPAPAADTQAVAGAEEMGGDAQAGQPRSDQPAQGARKRRQQPQRKAALVDGDNKPTSQQPGRRNKGGQRKGQ
QPGGKPGADRGDQRVEQRQGDKPGRQAGKGGAKTAAGGEDLFRFVISEQFDQEDTIVPRTKAKPVRELSADDDAPKLHKV
LAEGGLGSRREMEELILQGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVTTKPPRVLLYHKPSGEIVSQSDPEGRPTV
FDSLPRIKNGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAEADRQRLLHGIQLDDGEANFLR
IADGGGEGVNQWYHVALTEGRNREVRRMFEAVGLTVSRLIRTRYGQFLLPRGLKRGRWQELDPTDVRGLMANIGLKAPSK
EAQSGGKGVTKVGGRKQREEAALAGMPMHTGMDGLPRYEKSGGRGGNAGGAGRGQPDPMQTSMGYIPSGPAPLTSHTTKF
GGGMGGGLRGPGAGGGRGGGRGGKAGNAGNAGNAGGNRQRRGGGEVNGNVMPKAAKGAGGGSRRPRGRGQR
>Mature_630_residues
SDSAEQDVRQPDVPGDTGAAPEGNGAEGAPRRKGLRRGLRNLVASRRQAGQDRDGRETGRDDVEPSSTGESGDASPAAAP
ARGRGRRKPAPAADTQAVAGAEEMGGDAQAGQPRSDQPAQGARKRRQQPQRKAALVDGDNKPTSQQPGRRNKGGQRKGQQ
PGGKPGADRGDQRVEQRQGDKPGRQAGKGGAKTAAGGEDLFRFVISEQFDQEDTIVPRTKAKPVRELSADDDAPKLHKVL
AEGGLGSRREMEELILQGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVTTKPPRVLLYHKPSGEIVSQSDPEGRPTVF
DSLPRIKNGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAEADRQRLLHGIQLDDGEANFLRI
ADGGGEGVNQWYHVALTEGRNREVRRMFEAVGLTVSRLIRTRYGQFLLPRGLKRGRWQELDPTDVRGLMANIGLKAPSKE
AQSGGKGVTKVGGRKQREEAALAGMPMHTGMDGLPRYEKSGGRGGNAGGAGRGQPDPMQTSMGYIPSGPAPLTSHTTKFG
GGMGGGLRGPGAGGGRGGGRGGKAGNAGNAGNAGGNRQRRGGGEVNGNVMPKAAKGAGGGSRRPRGRGQR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=232, Percent_Identity=57.3275862068966, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790453, Length=251, Percent_Identity=29.8804780876494, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788510, Length=213, Percent_Identity=29.5774647887324, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=31.6062176165803, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 66760; Mature: 66628

Theoretical pI: Translated: 11.25; Mature: 11.25

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSAEQDVRQPDVPGDTGAAPEGNGAEGAPRRKGLRRGLRNLVASRRQAGQDRDGRETG
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCC
RDDVEPSSTGESGDASPAAAPARGRGRRKPAPAADTQAVAGAEEMGGDAQAGQPRSDQPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCHHHHCCCCCCCCCCCCCCH
QGARKRRQQPQRKAALVDGDNKPTSQQPGRRNKGGQRKGQQPGGKPGADRGDQRVEQRQG
HHHHHHHHCHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
DKPGRQAGKGGAKTAAGGEDLFRFVISEQFDQEDTIVPRTKAKPVRELSADDDAPKLHKV
CCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHH
LAEGGLGSRREMEELILQGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVTTKPPRVLL
HHHCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEECCEEEEEEECCCCCEEEE
YHKPSGEIVSQSDPEGRPTVFDSLPRIKNGKWVAVGRLDFNTEGLLIFTTSGDIANRFMH
EECCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHHCC
PRYGVEREYAVRTLGELAEADRQRLLHGIQLDDGEANFLRIADGGGEGVNQWYHVALTEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCC
RNREVRRMFEAVGLTVSRLIRTRYGQFLLPRGLKRGRWQELDPTDVRGLMANIGLKAPSK
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCH
EAQSGGKGVTKVGGRKQREEAALAGMPMHTGMDGLPRYEKSGGRGGNAGGAGRGQPDPMQ
HHHCCCCCCHHCCCCHHHHHHHHHCCCHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHH
TSMGYIPSGPAPLTSHTTKFGGGMGGGLRGPGAGGGRGGGRGGKAGNAGNAGNAGGNRQR
HHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RGGGEVNGNVMPKAAKGAGGGSRRPRGRGQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDSAEQDVRQPDVPGDTGAAPEGNGAEGAPRRKGLRRGLRNLVASRRQAGQDRDGRETG
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCC
RDDVEPSSTGESGDASPAAAPARGRGRRKPAPAADTQAVAGAEEMGGDAQAGQPRSDQPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCHHHHCCCCCCCCCCCCCCH
QGARKRRQQPQRKAALVDGDNKPTSQQPGRRNKGGQRKGQQPGGKPGADRGDQRVEQRQG
HHHHHHHHCHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
DKPGRQAGKGGAKTAAGGEDLFRFVISEQFDQEDTIVPRTKAKPVRELSADDDAPKLHKV
CCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHH
LAEGGLGSRREMEELILQGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVTTKPPRVLL
HHHCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEECCEEEEEEECCCCCEEEE
YHKPSGEIVSQSDPEGRPTVFDSLPRIKNGKWVAVGRLDFNTEGLLIFTTSGDIANRFMH
EECCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHHCC
PRYGVEREYAVRTLGELAEADRQRLLHGIQLDDGEANFLRIADGGGEGVNQWYHVALTEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCC
RNREVRRMFEAVGLTVSRLIRTRYGQFLLPRGLKRGRWQELDPTDVRGLMANIGLKAPSK
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCH
EAQSGGKGVTKVGGRKQREEAALAGMPMHTGMDGLPRYEKSGGRGGNAGGAGRGQPDPMQ
HHHCCCCCCHHCCCCHHHHHHHHHCCCHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHH
TSMGYIPSGPAPLTSHTTKFGGGMGGGLRGPGAGGGRGGGRGGKAGNAGNAGNAGGNRQR
HHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RGGGEVNGNVMPKAAKGAGGGSRRPRGRGQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]