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The map label for this gene is phaY

Identifier: 94310898

GI number: 94310898

Start: 2135352

End: 2137520

Strand: Reverse

Name: phaY

Synonym: Rmet_1960

Alternate gene names: NA

Gene position: 2137520-2135352 (Counterclockwise)

Preceding gene: 94310899

Following gene: 94310897

Centisome position: 54.42

GC content: 67.22

Gene sequence:

>2169_bases
ATGCAGCAGCGCCACCTCTCGCAGTCCGCCCATTCGCACGGCCATGGCACCCGGCGTGCGCACCGCCGAAACACGATCGC
CATCGCCGTCGCCACGCTTGCCGTCGCTGCGTGCGGCGGGTTTGGCACAAATGGGAGCGGCGGGAACACCGGTCCCAACG
TCAAGCCGGCGTTCATATCCGGCCCCGTCGGAGTCAAATCCTACGACGGCAACACGGATGACCTGCTGACGGCGGGGCTG
GGCAAGGACGGACTGGCGGCCGCAGCGCCGGCGTTTGCCAATCCGAACGCGCCGACGGCCGTGGAATTGCGCCGTAACGC
GATCTGGGCCAACTACCGCGCGATCGTGGATGTCCAGGCCGCAGGCGGCTATGGCTCGCTCTACGGCCCCAACGTCGACG
CCAAGGGGAATGTGACGACCGGCCAGGGCAAGGTCCCGGGTACCGAGTACATCGCATTTACCGACGACGGCTCGGGCCGG
CAAAACGTGACGGTCATGGCCCAGGTTCCGGATGGGTTCGATGTGAAACATCCCTGCATCATTTCCGCCACTTCGTCCGG
CTCGCGTGGTGTCTATGGCGGAATTGCCGTGGGGGAGTGGGGGCTCAAGCACAACTGCGCGGTGGCCTATACGGACAAGG
GCACCGGCGCGGCGCCGCATGACTTGGGTAGCGACACCGTGCCGCTGATCGACGGCACCCGGCAGACCCGGGCCGGTGCC
GGCACGCAGGCCCAGTTCGCGGCACAGCCCGGTGCCTCGGAAACGCTGGCGGGCTTCAATTCCAGCACCCCGCACCGGCT
TGCGTTCAAGCACGCGCACTCGCAGCAGAACCCGGAGGCGAACTGGGGCGCCAACACGTTGCAGGCGATCCAGTTCACGC
TCTGGGCGGTCAACGACAAGTTCGGCAGGACCAATACCGATGGCATGCGGCAGGCGACGTTCACGCCGGCCAATGTGCTG
GTGATCGCGTCGGCCATCTCGAATGGCGGCGGGGCGGCCATTGCAGCGGCGGAACAGGACACCGGCGGGTTGATCGACGG
CGTGGCCGTCGGGGAGCCGAATGTGAACCTGCCACCGACGGCAGGCGTGGTCGTCAAGCGCGGTGGCGTACCGGTGGCGG
CGTCGGGCAAGCATCTTTATGACTACACGACGATTGCCAACCTGTACCAGCTCTGCGCCAGCCAGGATGCCAGCCTGGGC
AATGCGCCATTTGCCAAAACGGCCGGGCAGGCCGCGCTCAACCGATGCGCGTCACTGGCCGCCAAGAAAATGATTGCCGG
AGGCAGCACATCCGCCCAGGCAACCTCCGCGCTGGATGCGTTGCACCGCGCCGGCTGGGAGCCGGAGTCGGATGACCTGT
TCGCGTCGCTTTCGGCGCTGGAGGTCGGCAGTTCCATTTCGGTGACCTATGCCAATGCCTATGCACGCGCCAGCGTGACG
GACCGGCTCTGCCACTACAGTTTTTCCTCGCCGGGCAGCCCGCCGCAGGCCAATCCCGTCACGGCCCCGGCGGCCGCGCT
GGCGGGGCTGTTCTCGACTGGCAATGGCATTCCGCCCACTACGGCGGTGACGCTGATGAACGATGCCAACCCGTCCGGCG
CGATGCGTGACTTCACGTCGATATCGCCCTCGACCAAGCTGGCCGACGGCAATGTCGACGGCGCGCTCTGCCTGCGTGGC
CTGCTGGATACCCGCAACCCGGCGCTGATGACCGGTATCGGCCAGACCTATCGCACGGGCAACCTTGGCGGCCGACCGTC
GGTCATCGTGCAGGGCCGCAGCGATGGCCTGCTGCCGGTGAACCACACGTCGCGGCCATATCTCGGGCTGAACAAGCACG
TCGAGGGCGCCAGCAGCCGGCTCTCGTACATCGAGGTGACCAACGGCCAGCATTTCGACGGGTTCATCGACGTGATTCCC
GGATATGCCAAGCGCTATATCCCGATGCACGTTTACGTGAACCGGGCGCTCGACGCGGTCTACGCCAACCTGCGCGACGG
TACGCCGCTGCCGCCGTCGCAGGTGGTGCGCACCACGCCGCGCGGAGGTGCCGACACCGACACGGTCGGCCCCAGGATCC
AGCCGTCGAACGTGCCGCCGATTGCCGCCACACCCGCGGCCGGCGACGCGATCACGGTCACCGGCTCGACCGTGGACGTG
CCCAACTGA

Upstream 100 bases:

>100_bases
TCCGCAATTCCCCCTGTTTCGCAGTTTGCAGTTGTCTCACCGAGTCCTGGACGGGATGGCGTTCGCGCCATCCCGCCGAT
AACAACAAGGAGACATACCC

Downstream 100 bases:

>100_bases
GACGTGGCCGCCAGGCCCGGCGATGCGGGTTACCCGTCATGCACTTGGCCGATACTTGGTCAATGTATGACGGGTGTATC
GCCGATGTCTTGAAATGTCA

Product: D-(-)-3-hydroxybutyrate oligomer hydrolase

Products: NA

Alternate protein names: 3HB-oligomer hydrolase; 3HBOH

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MQQRHLSQSAHSHGHGTRRAHRRNTIAIAVATLAVAACGGFGTNGSGGNTGPNVKPAFISGPVGVKSYDGNTDDLLTAGL
GKDGLAAAAPAFANPNAPTAVELRRNAIWANYRAIVDVQAAGGYGSLYGPNVDAKGNVTTGQGKVPGTEYIAFTDDGSGR
QNVTVMAQVPDGFDVKHPCIISATSSGSRGVYGGIAVGEWGLKHNCAVAYTDKGTGAAPHDLGSDTVPLIDGTRQTRAGA
GTQAQFAAQPGASETLAGFNSSTPHRLAFKHAHSQQNPEANWGANTLQAIQFTLWAVNDKFGRTNTDGMRQATFTPANVL
VIASAISNGGGAAIAAAEQDTGGLIDGVAVGEPNVNLPPTAGVVVKRGGVPVAASGKHLYDYTTIANLYQLCASQDASLG
NAPFAKTAGQAALNRCASLAAKKMIAGGSTSAQATSALDALHRAGWEPESDDLFASLSALEVGSSISVTYANAYARASVT
DRLCHYSFSSPGSPPQANPVTAPAAALAGLFSTGNGIPPTTAVTLMNDANPSGAMRDFTSISPSTKLADGNVDGALCLRG
LLDTRNPALMTGIGQTYRTGNLGGRPSVIVQGRSDGLLPVNHTSRPYLGLNKHVEGASSRLSYIEVTNGQHFDGFIDVIP
GYAKRYIPMHVYVNRALDAVYANLRDGTPLPPSQVVRTTPRGGADTDTVGPRIQPSNVPPIAATPAAGDAITVTGSTVDV
PN

Sequences:

>Translated_722_residues
MQQRHLSQSAHSHGHGTRRAHRRNTIAIAVATLAVAACGGFGTNGSGGNTGPNVKPAFISGPVGVKSYDGNTDDLLTAGL
GKDGLAAAAPAFANPNAPTAVELRRNAIWANYRAIVDVQAAGGYGSLYGPNVDAKGNVTTGQGKVPGTEYIAFTDDGSGR
QNVTVMAQVPDGFDVKHPCIISATSSGSRGVYGGIAVGEWGLKHNCAVAYTDKGTGAAPHDLGSDTVPLIDGTRQTRAGA
GTQAQFAAQPGASETLAGFNSSTPHRLAFKHAHSQQNPEANWGANTLQAIQFTLWAVNDKFGRTNTDGMRQATFTPANVL
VIASAISNGGGAAIAAAEQDTGGLIDGVAVGEPNVNLPPTAGVVVKRGGVPVAASGKHLYDYTTIANLYQLCASQDASLG
NAPFAKTAGQAALNRCASLAAKKMIAGGSTSAQATSALDALHRAGWEPESDDLFASLSALEVGSSISVTYANAYARASVT
DRLCHYSFSSPGSPPQANPVTAPAAALAGLFSTGNGIPPTTAVTLMNDANPSGAMRDFTSISPSTKLADGNVDGALCLRG
LLDTRNPALMTGIGQTYRTGNLGGRPSVIVQGRSDGLLPVNHTSRPYLGLNKHVEGASSRLSYIEVTNGQHFDGFIDVIP
GYAKRYIPMHVYVNRALDAVYANLRDGTPLPPSQVVRTTPRGGADTDTVGPRIQPSNVPPIAATPAAGDAITVTGSTVDV
PN
>Mature_722_residues
MQQRHLSQSAHSHGHGTRRAHRRNTIAIAVATLAVAACGGFGTNGSGGNTGPNVKPAFISGPVGVKSYDGNTDDLLTAGL
GKDGLAAAAPAFANPNAPTAVELRRNAIWANYRAIVDVQAAGGYGSLYGPNVDAKGNVTTGQGKVPGTEYIAFTDDGSGR
QNVTVMAQVPDGFDVKHPCIISATSSGSRGVYGGIAVGEWGLKHNCAVAYTDKGTGAAPHDLGSDTVPLIDGTRQTRAGA
GTQAQFAAQPGASETLAGFNSSTPHRLAFKHAHSQQNPEANWGANTLQAIQFTLWAVNDKFGRTNTDGMRQATFTPANVL
VIASAISNGGGAAIAAAEQDTGGLIDGVAVGEPNVNLPPTAGVVVKRGGVPVAASGKHLYDYTTIANLYQLCASQDASLG
NAPFAKTAGQAALNRCASLAAKKMIAGGSTSAQATSALDALHRAGWEPESDDLFASLSALEVGSSISVTYANAYARASVT
DRLCHYSFSSPGSPPQANPVTAPAAALAGLFSTGNGIPPTTAVTLMNDANPSGAMRDFTSISPSTKLADGNVDGALCLRG
LLDTRNPALMTGIGQTYRTGNLGGRPSVIVQGRSDGLLPVNHTSRPYLGLNKHVEGASSRLSYIEVTNGQHFDGFIDVIP
GYAKRYIPMHVYVNRALDAVYANLRDGTPLPPSQVVRTTPRGGADTDTVGPRIQPSNVPPIAATPAAGDAITVTGSTVDV
PN

Specific function: Participates in the degradation of poly-3- hydroxybutyrate (PHB). It works downstream of poly(3- hydroxybutyrate) depolymerase, hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-oligomers) into 3HB-monomers

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HBOH_RALME (Q1LLY7)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584108.1
- STRING:   Q1LLY7
- GeneID:   4038765
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1960
- eggNOG:   NOG67905
- HOGENOM:   HBG288747
- OMA:   NPEKDWG
- ProtClustDB:   CLSK896937
- BioCyc:   RMET266264:RMET_1960-MONOMER
- HAMAP:   MF_01906
- InterPro:   IPR016582
- PIRSF:   PIRSF011409

Pfam domain/function: PF10605 3HBOH

EC number: =3.1.1.22

Molecular weight: Translated: 73918; Mature: 73918

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: ACT_SITE 327-327

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRHLSQSAHSHGHGTRRAHRRNTIAIAVATLAVAACGGFGTNGSGGNTGPNVKPAFIS
CCCHHHHHHHHHCCCCHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEC
GPVGVKSYDGNTDDLLTAGLGKDGLAAAAPAFANPNAPTAVELRRNAIWANYRAIVDVQA
CCCCCCCCCCCCHHHEEECCCCCCCHHCCCCCCCCCCCCEEEEHHHEEEECEEEEEEEEE
AGGYGSLYGPNVDAKGNVTTGQGKVPGTEYIAFTDDGSGRQNVTVMAQVPDGFDVKHPCI
CCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCEE
ISATSSGSRGVYGGIAVGEWGLKHNCAVAYTDKGTGAAPHDLGSDTVPLIDGTRQTRAGA
EEECCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCHHCCCC
GTQAQFAAQPGASETLAGFNSSTPHRLAFKHAHSQQNPEANWGANTLQAIQFTLWAVNDK
CCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHEEEEEEEEEECCC
FGRTNTDGMRQATFTPANVLVIASAISNGGGAAIAAAEQDTGGLIDGVAVGEPNVNLPPT
CCCCCCCCCEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCC
AGVVVKRGGVPVAASGKHLYDYTTIANLYQLCASQDASLGNAPFAKTAGQAALNRCASLA
CCEEEECCCCCEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
AKKMIAGGSTSAQATSALDALHRAGWEPESDDLFASLSALEVGSSISVTYANAYARASVT
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHH
DRLCHYSFSSPGSPPQANPVTAPAAALAGLFSTGNGIPPTTAVTLMNDANPSGAMRDFTS
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHC
ISPSTKLADGNVDGALCLRGLLDTRNPALMTGIGQTYRTGNLGGRPSVIVQGRSDGLLPV
CCCCCEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCCEEEECCCCCEEEC
NHTSRPYLGLNKHVEGASSRLSYIEVTNGQHFDGFIDVIPGYAKRYIPMHVYVNRALDAV
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCHHHHEEEEEEEHHHHHHHH
YANLRDGTPLPPSQVVRTTPRGGADTDTVGPRIQPSNVPPIAATPAAGDAITVTGSTVDV
HHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCEECC
PN
CC
>Mature Secondary Structure
MQQRHLSQSAHSHGHGTRRAHRRNTIAIAVATLAVAACGGFGTNGSGGNTGPNVKPAFIS
CCCHHHHHHHHHCCCCHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEC
GPVGVKSYDGNTDDLLTAGLGKDGLAAAAPAFANPNAPTAVELRRNAIWANYRAIVDVQA
CCCCCCCCCCCCHHHEEECCCCCCCHHCCCCCCCCCCCCEEEEHHHEEEECEEEEEEEEE
AGGYGSLYGPNVDAKGNVTTGQGKVPGTEYIAFTDDGSGRQNVTVMAQVPDGFDVKHPCI
CCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCEE
ISATSSGSRGVYGGIAVGEWGLKHNCAVAYTDKGTGAAPHDLGSDTVPLIDGTRQTRAGA
EEECCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCHHCCCC
GTQAQFAAQPGASETLAGFNSSTPHRLAFKHAHSQQNPEANWGANTLQAIQFTLWAVNDK
CCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHEEEEEEEEEECCC
FGRTNTDGMRQATFTPANVLVIASAISNGGGAAIAAAEQDTGGLIDGVAVGEPNVNLPPT
CCCCCCCCCEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCC
AGVVVKRGGVPVAASGKHLYDYTTIANLYQLCASQDASLGNAPFAKTAGQAALNRCASLA
CCEEEECCCCCEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
AKKMIAGGSTSAQATSALDALHRAGWEPESDDLFASLSALEVGSSISVTYANAYARASVT
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHH
DRLCHYSFSSPGSPPQANPVTAPAAALAGLFSTGNGIPPTTAVTLMNDANPSGAMRDFTS
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHC
ISPSTKLADGNVDGALCLRGLLDTRNPALMTGIGQTYRTGNLGGRPSVIVQGRSDGLLPV
CCCCCEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCCEEEECCCCCEEEC
NHTSRPYLGLNKHVEGASSRLSYIEVTNGQHFDGFIDVIPGYAKRYIPMHVYVNRALDAV
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCHHHHEEEEEEEHHHHHHHH
YANLRDGTPLPPSQVVRTTPRGGADTDTVGPRIQPSNVPPIAATPAAGDAITVTGSTVDV
HHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCEECC
PN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA