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The map label for this gene is ispF

Identifier: 94310891

GI number: 94310891

Start: 2123485

End: 2123991

Strand: Reverse

Name: ispF

Synonym: Rmet_1953

Alternate gene names: 94310891

Gene position: 2123991-2123485 (Counterclockwise)

Preceding gene: 94310892

Following gene: 335055566

Centisome position: 54.07

GC content: 63.71

Gene sequence:

>507_bases
TTGAACCCGTTCGATATCCGCATTGGCCAAGGCTATGACGTTCACGCCCTGGTGCCGGGCCGCAAGCTGATCCTCGGTGG
CGTGGACATTCCACACGATCGCGGACTGCTCGGTCATTCCGATGCCGACGCGCTGCTGCATGCCGTGACAGATGCGTTGT
TCGGTGCCGCGGCGCTGGGCGATATTGGTCGCCACTTTCCCGATACCGATCCGAATTTCGCTGGCGCCGACAGCCGGGTA
TTGCTGCGTGAGGCGGTCCGTCGTGTGCGCGAAGCCGGTTACGAGATCGGCAACGTCGACGCCACGGTCATCGCGCAGAA
GCCCAAGCTGGCGCCGCATATCAGCGGCATGGTGGCCAATCTCGCGGAAGATCTCGGCCTGCCCGTCACGCGCTGCAACG
TGAAGGCGAAGACCAACGAGAAACTGGGCTTCGAAGGAAAGGAGGAGGGGATCGTGGCGCAGGCGGCGGTACTGATCTAC
CGCCGCAACGGCGACGCCCAGGACTGA

Upstream 100 bases:

>100_bases
CATACCCCGAGGATTTCGCGCTGGCGGACGTACTGTTGCGCACTGGCACACCGACTTCCAACACGTGACAAACGTGATCC
ATCCAGACAAAGGAGCCGCA

Downstream 100 bases:

>100_bases
GCTTGTCGCCTGAAGTCTGAAATCGCGCCGTTGGCCGTCAGGCCTTCGGCGCTGTAGCTTCCGCCGTTTCGGCGGTGATC
GGATAGACGTAGTCGATCAG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MNPFDIRIGQGYDVHALVPGRKLILGGVDIPHDRGLLGHSDADALLHAVTDALFGAAALGDIGRHFPDTDPNFAGADSRV
LLREAVRRVREAGYEIGNVDATVIAQKPKLAPHISGMVANLAEDLGLPVTRCNVKAKTNEKLGFEGKEEGIVAQAAVLIY
RRNGDAQD

Sequences:

>Translated_168_residues
MNPFDIRIGQGYDVHALVPGRKLILGGVDIPHDRGLLGHSDADALLHAVTDALFGAAALGDIGRHFPDTDPNFAGADSRV
LLREAVRRVREAGYEIGNVDATVIAQKPKLAPHISGMVANLAEDLGLPVTRCNVKAKTNEKLGFEGKEEGIVAQAAVLIY
RRNGDAQD
>Mature_168_residues
MNPFDIRIGQGYDVHALVPGRKLILGGVDIPHDRGLLGHSDADALLHAVTDALFGAAALGDIGRHFPDTDPNFAGADSRV
LLREAVRRVREAGYEIGNVDATVIAQKPKLAPHISGMVANLAEDLGLPVTRCNVKAKTNEKLGFEGKEEGIVAQAAVLIY
RRNGDAQD

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=59.6153846153846, Blast_Score=179, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_RALME (Q1LLZ4)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584101.1
- ProteinModelPortal:   Q1LLZ4
- SMR:   Q1LLZ4
- STRING:   Q1LLZ4
- GeneID:   4038758
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_1953
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q1LLZ4
- ProtClustDB:   PRK00084
- BioCyc:   RMET266264:RMET_1953-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17909; Mature: 17909

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPFDIRIGQGYDVHALVPGRKLILGGVDIPHDRGLLGHSDADALLHAVTDALFGAAALG
CCCCEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DIGRHFPDTDPNFAGADSRVLLREAVRRVREAGYEIGNVDATVIAQKPKLAPHISGMVAN
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHH
LAEDLGLPVTRCNVKAKTNEKLGFEGKEEGIVAQAAVLIYRRNGDAQD
HHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHEEEEEEEEECCCCCCC
>Mature Secondary Structure
MNPFDIRIGQGYDVHALVPGRKLILGGVDIPHDRGLLGHSDADALLHAVTDALFGAAALG
CCCCEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DIGRHFPDTDPNFAGADSRVLLREAVRRVREAGYEIGNVDATVIAQKPKLAPHISGMVAN
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHH
LAEDLGLPVTRCNVKAKTNEKLGFEGKEEGIVAQAAVLIYRRNGDAQD
HHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA