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The map label for this gene is ispD [H]

Identifier: 94310892

GI number: 94310892

Start: 2124024

End: 2124848

Strand: Reverse

Name: ispD [H]

Synonym: Rmet_1954

Alternate gene names: 94310892

Gene position: 2124848-2124024 (Counterclockwise)

Preceding gene: 94310895

Following gene: 94310891

Centisome position: 54.09

GC content: 67.03

Gene sequence:

>825_bases
ATGGCGGCGGGCGAGGGCCGTATTATAGAATGCACCCCGGCCGGTTTGCCGGCCGGTTTCCGATATTCCATCTCCCGCAA
ATCCGTGTCAGATCGCCGCTTCGCCCTGATTCCCTGTGCCGGTACCGGCAGTCGTGCCGGAGGCCCCATCCAGAAGCAGT
ACCAGACCGTGGCCGGACGGCCGATGCTTTGGTATGCGCTGGCCGCCTTTTCCGCTTGCGATGCCATCAGCGCCACCGCG
CTGGTGCTGGCCCCCGACGACATGCCGCTCGAGTCCCGTTTCGGCGCCGCCGCCTTTGCCGGGCTCCGCTTCGATACAGC
GTTCGTCGGCGGTGATTCCCGCCATGCCTCGGTACTCGCCGGTCTCCATCATCTTGGGACCATGGGCGCAGTGGATACCG
ACTGGGTGCTCGTGCACGATGCGGCCCGGCCCGGCCTGACGCCCGCGATGATCGACACACTTGTGCGCGCTGTCGAGTCC
GACGATGCCGCCAGCGAGCCGATCGGCGGCATTCTTGCCGTGCCGGTGCCGGACACGCTCAAGCGTGCCGACGCCGCTGC
CCGCATCGGCGCCACCGTGCCGCGCGAAGGACTGTGGCAAGCCCAGACACCGCAGATGTTCCGAATCGGCGTGCTGCGCC
ATGCGCTGGAGGATGCGCTGGCCGCGGGCGCGGTGGTGACCGACGAGGCGAGTGCGATCGAGCGGCTTGGACTGCATCCG
CGCCTGGTGAATGGTTCGCTGCGCAACTTCAAGGTGACATACCCCGAGGATTTCGCGCTGGCGGACGTACTGTTGCGCAC
TGGCACACCGACTTCCAACACGTGA

Upstream 100 bases:

>100_bases
CCGGCGTTTGCGAGGGCCCGTTGTTGCCCTGTGACGGGCTGGGCCCGGGCTGTTCACGGGCGAGGATCATGGTTCATGCC
CACTGCATCGGCAGTGCCAG

Downstream 100 bases:

>100_bases
CAAACGTGATCCATCCAGACAAAGGAGCCGCATTGAACCCGTTCGATATCCGCATTGGCCAAGGCTATGACGTTCACGCC
CTGGTGCCGGGCCGCAAGCT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MAAGEGRIIECTPAGLPAGFRYSISRKSVSDRRFALIPCAGTGSRAGGPIQKQYQTVAGRPMLWYALAAFSACDAISATA
LVLAPDDMPLESRFGAAAFAGLRFDTAFVGGDSRHASVLAGLHHLGTMGAVDTDWVLVHDAARPGLTPAMIDTLVRAVES
DDAASEPIGGILAVPVPDTLKRADAAARIGATVPREGLWQAQTPQMFRIGVLRHALEDALAAGAVVTDEASAIERLGLHP
RLVNGSLRNFKVTYPEDFALADVLLRTGTPTSNT

Sequences:

>Translated_274_residues
MAAGEGRIIECTPAGLPAGFRYSISRKSVSDRRFALIPCAGTGSRAGGPIQKQYQTVAGRPMLWYALAAFSACDAISATA
LVLAPDDMPLESRFGAAAFAGLRFDTAFVGGDSRHASVLAGLHHLGTMGAVDTDWVLVHDAARPGLTPAMIDTLVRAVES
DDAASEPIGGILAVPVPDTLKRADAAARIGATVPREGLWQAQTPQMFRIGVLRHALEDALAAGAVVTDEASAIERLGLHP
RLVNGSLRNFKVTYPEDFALADVLLRTGTPTSNT
>Mature_273_residues
AAGEGRIIECTPAGLPAGFRYSISRKSVSDRRFALIPCAGTGSRAGGPIQKQYQTVAGRPMLWYALAAFSACDAISATAL
VLAPDDMPLESRFGAAAFAGLRFDTAFVGGDSRHASVLAGLHHLGTMGAVDTDWVLVHDAARPGLTPAMIDTLVRAVESD
DAASEPIGGILAVPVPDTLKRADAAARIGATVPREGLWQAQTPQMFRIGVLRHALEDALAAGAVVTDEASAIERLGLHPR
LVNGSLRNFKVTYPEDFALADVLLRTGTPTSNT

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=241, Percent_Identity=41.9087136929461, Blast_Score=150, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 28714; Mature: 28583

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGEGRIIECTPAGLPAGFRYSISRKSVSDRRFALIPCAGTGSRAGGPIQKQYQTVAGR
CCCCCCCEEEECCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCC
PMLWYALAAFSACDAISATALVLAPDDMPLESRFGAAAFAGLRFDTAFVGGDSRHASVLA
CHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCHHHHHCCEEEEEEECCCCHHHHHHH
GLHHLGTMGAVDTDWVLVHDAARPGLTPAMIDTLVRAVESDDAASEPIGGILAVPVPDTL
HHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHH
KRADAAARIGATVPREGLWQAQTPQMFRIGVLRHALEDALAAGAVVTDEASAIERLGLHP
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCC
RLVNGSLRNFKVTYPEDFALADVLLRTGTPTSNT
EEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AAGEGRIIECTPAGLPAGFRYSISRKSVSDRRFALIPCAGTGSRAGGPIQKQYQTVAGR
CCCCCCEEEECCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCC
PMLWYALAAFSACDAISATALVLAPDDMPLESRFGAAAFAGLRFDTAFVGGDSRHASVLA
CHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCHHHHHCCEEEEEEECCCCHHHHHHH
GLHHLGTMGAVDTDWVLVHDAARPGLTPAMIDTLVRAVESDDAASEPIGGILAVPVPDTL
HHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHH
KRADAAARIGATVPREGLWQAQTPQMFRIGVLRHALEDALAAGAVVTDEASAIERLGLHP
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCC
RLVNGSLRNFKVTYPEDFALADVLLRTGTPTSNT
EEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA