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The map label for this gene is benM [H]

Identifier: 94310877

GI number: 94310877

Start: 2107731

End: 2108669

Strand: Reverse

Name: benM [H]

Synonym: Rmet_1939

Alternate gene names: 94310877

Gene position: 2108669-2107731 (Counterclockwise)

Preceding gene: 94310880

Following gene: 94310869

Centisome position: 53.68

GC content: 66.77

Gene sequence:

>939_bases
ATGGCAGCCAAAGTCAATGAAGCGCGTGGCACACTCATGCTTATGACGCCCTCCCTGAAGCAGTTGCGCTATTTCGTCGA
GATTGTGGACGCAGGCAATTTCAGCCTGGCGGCCGAACGGCTGTTCATCGCCCAATCGGCGCTGAGCCGGCAGATACGTG
ACATGGAAGCGACGCTGCAGGTGGAACTGCTGGTGCGCAAGCCTCGGCACGTGGAGCCGACGGCGGCGGGGCAGGCATTC
TACGAATCGGCGCGCCGCATTCTGTCCGACCTCGCCGGCGCAGCCGTGCAGGCCCGCCAAGTGCAGCGCGGTGGGGCCGA
TGCGATACGCCTGCTCCATTCGAGTTCGGTCGTGCTGACGCCGCAGCGTTGCGGCTGGTTGCATCGCTGGCAGTCCGACC
ATCCCGCCGCCCGCTTTGAAGTTGCCCAGGCATCGTCGGAGCAGCAATCGGTCGACATCGAGGAGGGCCGTGCCGACCTC
GGGTTGGCGCGTGATCCGGTACTGCGCCGCTACCCGAACATCCAGATCGATCCGATGGCCGGGGAGCGGCTGGTCGTGGC
TGTCTGCGCCGATCATCCGATGTCGCGACGGGAATCGCTGACGCTGAGTGCCTTGCGAGAGATGCCGTTTGTCTCGCTGC
CGCACCCGGAGCGGGGCGGGCTCAGCTACCGCGTGGCGCAGCTTTGCCAGGCGAGCGGCTTCTATCCAACGGCGGCCGGT
GCGATTTCGCGCAAGCTTTCGCAGCTATCTCTTGTGGCGGCGGGCTTTGGCGTAGCCGTGGTGCCGGAATCGATGCAGTG
GTTCGGGCCCGATGGCGTCCGGTTGGTGCCACTGTCCGATGCGGGCGCGACGACACGTGTGGTACTGCTCTCCCGCCGAG
ATGCGCCCGAGCCGGTGGCGGCGCTCCGTACGCTGGCGCTGTCCGCCGGCGCCGCCTGA

Upstream 100 bases:

>100_bases
CAGGTCCATCGGCGAGGCGGGGATTGGGAGAGGATTCAGCGTAGCGCCGCGCGACGGGCCGAGCGATCGCAAATGCCGTG
CGCTGCCATCGCAAATCGGC

Downstream 100 bases:

>100_bases
ACCCGTCAGGCCAGTTCGAATACGACGTCCTGGGCGCCTTCCCACAGCACCTTGGTGCCCAGGGCGCGCAGGTTTTCCTT
GCCCTCGACGATGGTCAGGA

Product: transcriptional regulator, lysR family

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MAAKVNEARGTLMLMTPSLKQLRYFVEIVDAGNFSLAAERLFIAQSALSRQIRDMEATLQVELLVRKPRHVEPTAAGQAF
YESARRILSDLAGAAVQARQVQRGGADAIRLLHSSSVVLTPQRCGWLHRWQSDHPAARFEVAQASSEQQSVDIEEGRADL
GLARDPVLRRYPNIQIDPMAGERLVVAVCADHPMSRRESLTLSALREMPFVSLPHPERGGLSYRVAQLCQASGFYPTAAG
AISRKLSQLSLVAAGFGVAVVPESMQWFGPDGVRLVPLSDAGATTRVVLLSRRDAPEPVAALRTLALSAGAA

Sequences:

>Translated_312_residues
MAAKVNEARGTLMLMTPSLKQLRYFVEIVDAGNFSLAAERLFIAQSALSRQIRDMEATLQVELLVRKPRHVEPTAAGQAF
YESARRILSDLAGAAVQARQVQRGGADAIRLLHSSSVVLTPQRCGWLHRWQSDHPAARFEVAQASSEQQSVDIEEGRADL
GLARDPVLRRYPNIQIDPMAGERLVVAVCADHPMSRRESLTLSALREMPFVSLPHPERGGLSYRVAQLCQASGFYPTAAG
AISRKLSQLSLVAAGFGVAVVPESMQWFGPDGVRLVPLSDAGATTRVVLLSRRDAPEPVAALRTLALSAGAA
>Mature_311_residues
AAKVNEARGTLMLMTPSLKQLRYFVEIVDAGNFSLAAERLFIAQSALSRQIRDMEATLQVELLVRKPRHVEPTAAGQAFY
ESARRILSDLAGAAVQARQVQRGGADAIRLLHSSSVVLTPQRCGWLHRWQSDHPAARFEVAQASSEQQSVDIEEGRADLG
LARDPVLRRYPNIQIDPMAGERLVVAVCADHPMSRRESLTLSALREMPFVSLPHPERGGLSYRVAQLCQASGFYPTAAGA
ISRKLSQLSLVAAGFGVAVVPESMQWFGPDGVRLVPLSDAGATTRVVLLSRRDAPEPVAALRTLALSAGAA

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=33.5664335664336, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1787879, Length=276, Percent_Identity=28.9855072463768, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI2367136, Length=270, Percent_Identity=25.9259259259259, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=24.390243902439, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787530, Length=252, Percent_Identity=26.5873015873016, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786713, Length=252, Percent_Identity=26.984126984127, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=26.5060240963855, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=295, Percent_Identity=25.4237288135593, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=312, Percent_Identity=24.6794871794872, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33796; Mature: 33665

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKVNEARGTLMLMTPSLKQLRYFVEIVDAGNFSLAAERLFIAQSALSRQIRDMEATLQ
CCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHE
VELLVRKPRHVEPTAAGQAFYESARRILSDLAGAAVQARQVQRGGADAIRLLHSSSVVLT
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEC
PQRCGWLHRWQSDHPAARFEVAQASSEQQSVDIEEGRADLGLARDPVLRRYPNIQIDPMA
HHHCCHHHCCCCCCCHHHEEHHHCCCCHHCCCHHCCCCCCCCCCCCHHHHCCCCEECCCC
GERLVVAVCADHPMSRRESLTLSALREMPFVSLPHPERGGLSYRVAQLCQASGFYPTAAG
CCEEEEEEECCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCCHHHH
AISRKLSQLSLVAAGFGVAVVPESMQWFGPDGVRLVPLSDAGATTRVVLLSRRDAPEPVA
HHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHH
ALRTLALSAGAA
HHHHHHHHCCCC
>Mature Secondary Structure 
AAKVNEARGTLMLMTPSLKQLRYFVEIVDAGNFSLAAERLFIAQSALSRQIRDMEATLQ
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHE
VELLVRKPRHVEPTAAGQAFYESARRILSDLAGAAVQARQVQRGGADAIRLLHSSSVVLT
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEC
PQRCGWLHRWQSDHPAARFEVAQASSEQQSVDIEEGRADLGLARDPVLRRYPNIQIDPMA
HHHCCHHHCCCCCCCHHHEEHHHCCCCHHCCCHHCCCCCCCCCCCCHHHHCCCCEECCCC
GERLVVAVCADHPMSRRESLTLSALREMPFVSLPHPERGGLSYRVAQLCQASGFYPTAAG
CCEEEEEEECCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHCCCCCHHHH
AISRKLSQLSLVAAGFGVAVVPESMQWFGPDGVRLVPLSDAGATTRVVLLSRRDAPEPVA
HHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHH
ALRTLALSAGAA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]