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The map label for this gene is amaB-2 [H]

Identifier: 94310869

GI number: 94310869

Start: 2098858

End: 2100105

Strand: Reverse

Name: amaB-2 [H]

Synonym: Rmet_1931

Alternate gene names: 94310869

Gene position: 2100105-2098858 (Counterclockwise)

Preceding gene: 94310877

Following gene: 94310867

Centisome position: 53.46

GC content: 64.9

Gene sequence:

>1248_bases
ATGGATTCGCAATTCACTCCTCGCATCAATGGCGCACGCCTGTGGCAATCGCTGATGGATCTGGGCGCGCTCGGCGCCAC
GCCTAAGGGTGGCGTGTGCCGCATTGCGCTGACCGAAGACGACCGAAAGGGCCGCGACCTCGTGGCGAGCTGGTGCCGCG
AGGCTGGGCTCGACGTGCGCGTGGACGAGATCGGCAATGTGTTCGCACGACGTGCCGGCACAGACCCCACCGCACCAGCG
GTGGCCACGGGAAGCCATATCGATACCCAACCGTCCGGCGGCAAGTTCGACGGCAATTTCGGCGTGCTGGCGGGTCTGGA
AGTGATGCGCACGCTCAACGATCTGGGCATTGCCACACGTGCACCGCTGGAGGTGGCGATCTGGACCAACGAGGAAGGCA
CGCGCTTTACGCCCGTGATGATGGGATCGGGCGTATTCGCGGGCGTATTCGATGCGGAGTTCGCCAGACGGCAGCAAGAC
CGAGACGGCGTGAGTGTTGGTGATGCGCTCGCAGCTATCGGCTATCGCGGCACGCAGCGTGCGGGTGAAGTGCCCGGTGG
CATGTATTCGGCCTACTTCGAGGCGCATATCGAGCAAGGGCCAGTCCTGGAGGCGGCTGGCCTACCGATCGGCGTGGTAT
CGGGGGCACTTGGGCAGCAATGGTACGACGTGACGGTCACCGGCCAGGATGCGCATGCAGGCCCGACGCCGCTGGCCTTG
CGTCACGACGCGCTGCTGGCTTCGGCGCGCATGATCGAGGCGGTGAATCGCATTGCGCGCGACGAAGCGCCGCATGGCCG
CGGCACGGTGGGCTTCCTGGAGGTCACGCCGAACTCGCGCAACGTGATACCAGGGCGCGTGGATTTCTCGGTGGATGTTC
GTCATCTCTCGCAAGATGGCCTTGACCGGATGGACGCAGCGATGCGTGGGGCGTTCGCCGGTATTGCCGATGCGGCGGCC
GTAGACGTGGAGATTCGGCAGGTGGTCAAGTTCGAACCCTGCCTGTTCGCACCGGCGTGCGTCGATAGCGTTCGCCGCGC
GGCGGTTGGGTTGGATCTGCCGCACATGGATGTGGTCAGTGGCGCCGGACATGATGCGGTGTACGTGGCACAGCGCGCAC
CCACTGGCATGATCTTCGTACCGTGCAAGGACGGCATCAGCCATAACGAGCTGGAGGACGCGCTGCCCGAGCATATCGAA
GCCGGTGCCAACGTCCTGCTGCGAGCCATGCTGGAACACGCGCGATAA

Upstream 100 bases:

>100_bases
CCATCGACGGGTGGTCATGGCCGAGACTGTCCGCACCTGTCAGTGGTCTGCTTCTTGCTCGTACCCAGGTTGATGTTCAC
TCACTGCAGACAACACAGAC

Downstream 100 bases:

>100_bases
GCGCCTGCCGGCTCAGTGGGCGATCGATCGCGAGATCGCCCACGCGCATTCGACCACCAGCGGGCCGAGCTTTTTCTGCG
CGTCGACCATGGTCCAGCGG

Product: N-carbamoyl-L-amino-acid hydrolase

Products: NA

Alternate protein names: L-carbamoylase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MDSQFTPRINGARLWQSLMDLGALGATPKGGVCRIALTEDDRKGRDLVASWCREAGLDVRVDEIGNVFARRAGTDPTAPA
VATGSHIDTQPSGGKFDGNFGVLAGLEVMRTLNDLGIATRAPLEVAIWTNEEGTRFTPVMMGSGVFAGVFDAEFARRQQD
RDGVSVGDALAAIGYRGTQRAGEVPGGMYSAYFEAHIEQGPVLEAAGLPIGVVSGALGQQWYDVTVTGQDAHAGPTPLAL
RHDALLASARMIEAVNRIARDEAPHGRGTVGFLEVTPNSRNVIPGRVDFSVDVRHLSQDGLDRMDAAMRGAFAGIADAAA
VDVEIRQVVKFEPCLFAPACVDSVRRAAVGLDLPHMDVVSGAGHDAVYVAQRAPTGMIFVPCKDGISHNELEDALPEHIE
AGANVLLRAMLEHAR

Sequences:

>Translated_415_residues
MDSQFTPRINGARLWQSLMDLGALGATPKGGVCRIALTEDDRKGRDLVASWCREAGLDVRVDEIGNVFARRAGTDPTAPA
VATGSHIDTQPSGGKFDGNFGVLAGLEVMRTLNDLGIATRAPLEVAIWTNEEGTRFTPVMMGSGVFAGVFDAEFARRQQD
RDGVSVGDALAAIGYRGTQRAGEVPGGMYSAYFEAHIEQGPVLEAAGLPIGVVSGALGQQWYDVTVTGQDAHAGPTPLAL
RHDALLASARMIEAVNRIARDEAPHGRGTVGFLEVTPNSRNVIPGRVDFSVDVRHLSQDGLDRMDAAMRGAFAGIADAAA
VDVEIRQVVKFEPCLFAPACVDSVRRAAVGLDLPHMDVVSGAGHDAVYVAQRAPTGMIFVPCKDGISHNELEDALPEHIE
AGANVLLRAMLEHAR
>Mature_415_residues
MDSQFTPRINGARLWQSLMDLGALGATPKGGVCRIALTEDDRKGRDLVASWCREAGLDVRVDEIGNVFARRAGTDPTAPA
VATGSHIDTQPSGGKFDGNFGVLAGLEVMRTLNDLGIATRAPLEVAIWTNEEGTRFTPVMMGSGVFAGVFDAEFARRQQD
RDGVSVGDALAAIGYRGTQRAGEVPGGMYSAYFEAHIEQGPVLEAAGLPIGVVSGALGQQWYDVTVTGQDAHAGPTPLAL
RHDALLASARMIEAVNRIARDEAPHGRGTVGFLEVTPNSRNVIPGRVDFSVDVRHLSQDGLDRMDAAMRGAFAGIADAAA
VDVEIRQVVKFEPCLFAPACVDSVRRAAVGLDLPHMDVVSGAGHDAVYVAQRAPTGMIFVPCKDGISHNELEDALPEHIE
AGANVLLRAMLEHAR

Specific function: Involved In The Anaerobic Utilization Of Allantoin. Converts Allantoate To (S)-Ureidoglycolate And Ammonia. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=378, Percent_Identity=34.6560846560847, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.87 [H]

Molecular weight: Translated: 43994; Mature: 43994

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSQFTPRINGARLWQSLMDLGALGATPKGGVCRIALTEDDRKGRDLVASWCREAGLDVR
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEE
VDEIGNVFARRAGTDPTAPAVATGSHIDTQPSGGKFDGNFGVLAGLEVMRTLNDLGIATR
HHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
APLEVAIWTNEEGTRFTPVMMGSGVFAGVFDAEFARRQQDRDGVSVGDALAAIGYRGTQR
CCEEEEEEECCCCCEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
AGEVPGGMYSAYFEAHIEQGPVLEAAGLPIGVVSGALGQQWYDVTVTGQDAHAGPTPLAL
CCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCEEE
RHDALLASARMIEAVNRIARDEAPHGRGTVGFLEVTPNSRNVIPGRVDFSVDVRHLSQDG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEHHHHHHCC
LDRMDAAMRGAFAGIADAAAVDVEIRQVVKFEPCLFAPACVDSVRRAAVGLDLPHMDVVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCHHHHC
GAGHDAVYVAQRAPTGMIFVPCKDGISHNELEDALPEHIEAGANVLLRAMLEHAR
CCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSQFTPRINGARLWQSLMDLGALGATPKGGVCRIALTEDDRKGRDLVASWCREAGLDVR
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEE
VDEIGNVFARRAGTDPTAPAVATGSHIDTQPSGGKFDGNFGVLAGLEVMRTLNDLGIATR
HHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
APLEVAIWTNEEGTRFTPVMMGSGVFAGVFDAEFARRQQDRDGVSVGDALAAIGYRGTQR
CCEEEEEEECCCCCEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
AGEVPGGMYSAYFEAHIEQGPVLEAAGLPIGVVSGALGQQWYDVTVTGQDAHAGPTPLAL
CCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCEEE
RHDALLASARMIEAVNRIARDEAPHGRGTVGFLEVTPNSRNVIPGRVDFSVDVRHLSQDG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEHHHHHHCC
LDRMDAAMRGAFAGIADAAAVDVEIRQVVKFEPCLFAPACVDSVRRAAVGLDLPHMDVVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCHHHHC
GAGHDAVYVAQRAPTGMIFVPCKDGISHNELEDALPEHIEAGANVLLRAMLEHAR
CCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9023955; 8285691 [H]