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The map label for this gene is gatA [H]

Identifier: 94310811

GI number: 94310811

Start: 2033361

End: 2034713

Strand: Direct

Name: gatA [H]

Synonym: Rmet_1873

Alternate gene names: 94310811

Gene position: 2033361-2034713 (Clockwise)

Preceding gene: 94310810

Following gene: 94310812

Centisome position: 51.76

GC content: 69.25

Gene sequence:

>1353_bases
ATGCTGCCTGACATCGCCTCCCTGAACGCCGCCGTCCGCGCCGGCACCACCAGCCGCGCCGACCTGCTTGCCGAAGCCGC
CACCAAGGCCACCAGCGCCGCCGGCCACGCGGTCTATCTCCATACCACGTTCGATGCCGCCATCGAGGTGGCCCGCGCGG
CCGATGCCGCCGACCGCGCCGGCAAACCGCTCCACCCACTGGCGGGCCTGCCTGTATCGGTCAAGGATCTCTACGACATC
GCGGGCGAGGTCACGCGCGCCGCGTCTGCCGCCCGCCAGGACGCCGCCCCCGCAACGGCCGATGCCACCGTGGTGGCCCG
GCTCCGCGCGGCCGGCGCCGCGCTGATCGGCCGCACCAACATGACCGAGTTCGCCTTTTCTGGCGTCGGCATCAATCCCC
ATTTCGGCACGCCGGTCAACCCATGCGACAGCTCGGTTGCACGGATTCCGGGCGGATCGTCGTCTGGGGCGGCGGTCTCG
GTCGCGATCGGCACGGCGGTGGCCGGCCTCGGCAGCGACACGGGCGGCTCGATCCGCATTCCCGCCGCGCTATGCGGCAT
CGTCGGCTTCAAACCCACATCGCGCCGCGTGCCGCTGACGGGCGCGTTCCCGCTGTCCTACACGCTCGATACCGCGTGTG
CGATGGCGCGCACGCTGACCGATTGCATCGCCGTGGATAGCGTCATCGCCGACGTCCCGTTCATTCCGCAAATGAAGGCC
TCCACGGCGATCCACCTGGCGATTCCGCGCCAGCTGGTTCTCGACGATCTGGACGAGCCGGTCTCGAAAGCCTTCGACCG
TGCCATCGGCCGGCTTTCCGCGGCCGGCGTGCGCATCGAGCACGTGGACATGCCCGAACTGACCGAACTGGCCAGCCTGA
ATGCCGCTGGCGGCTTTTCGGCGCCCGAGTCCTACGCGCACCATCGCGCGCTGCTCGCCATCCACCGCGATCTGTACGAC
CCGCGCGTGGTGACCCGCATCGAACGGGGCGCGGCGATGAGCGCGGCGGACTACATCGCACTGCACCGCGCCCGACTCGA
CTGGATTGCCCGCATGGAAGCTCGCATGGCACCGTTCGATGCGGTGGTCTGCCCCACGGTGCCAATGGTGGCGCCGCCGA
TCGCTCCGCTGGTGGAAGATGACACCCTGTTCTTCCGCACAAACGGGCTGCTCCTGCGCAATACGTCCGCGTTCAATTTT
CTCGACGGCTGTTCGGTGTCGCTGCCCTGCCATGCGCCCGACGAACTGCCCGTCGGCCTGATGCTTTCCCAGGGACCGAT
GCGCGACGCGCAGCTGCTGGGCACGGCGCTTGCATTGGAATCGCTCGTGCACCCCTCACGGCGCGGAGGCTGA

Upstream 100 bases:

>100_bases
CCTTGTCGGATTCTTGAGCTTCTTGATTCACACAGGCAATGCGTGAATGTGATTTTGCTCCGCGGCGCCTGTCACTCTCG
TCTCGCCCACGCAAACCGTC

Downstream 100 bases:

>100_bases
GCCGCCGCCGCACCGCGACACGCATTACCGCATTACCGCCGAGATCACCAACAACCCGGGAATCGGCGCAGGTAGTTCCA
GGAGGATAGTCAAACCCGCG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLPDIASLNAAVRAGTTSRADLLAEAATKATSAAGHAVYLHTTFDAAIEVARAADAADRAGKPLHPLAGLPVSVKDLYDI
AGEVTRAASAARQDAAPATADATVVARLRAAGAALIGRTNMTEFAFSGVGINPHFGTPVNPCDSSVARIPGGSSSGAAVS
VAIGTAVAGLGSDTGGSIRIPAALCGIVGFKPTSRRVPLTGAFPLSYTLDTACAMARTLTDCIAVDSVIADVPFIPQMKA
STAIHLAIPRQLVLDDLDEPVSKAFDRAIGRLSAAGVRIEHVDMPELTELASLNAAGGFSAPESYAHHRALLAIHRDLYD
PRVVTRIERGAAMSAADYIALHRARLDWIARMEARMAPFDAVVCPTVPMVAPPIAPLVEDDTLFFRTNGLLLRNTSAFNF
LDGCSVSLPCHAPDELPVGLMLSQGPMRDAQLLGTALALESLVHPSRRGG

Sequences:

>Translated_450_residues
MLPDIASLNAAVRAGTTSRADLLAEAATKATSAAGHAVYLHTTFDAAIEVARAADAADRAGKPLHPLAGLPVSVKDLYDI
AGEVTRAASAARQDAAPATADATVVARLRAAGAALIGRTNMTEFAFSGVGINPHFGTPVNPCDSSVARIPGGSSSGAAVS
VAIGTAVAGLGSDTGGSIRIPAALCGIVGFKPTSRRVPLTGAFPLSYTLDTACAMARTLTDCIAVDSVIADVPFIPQMKA
STAIHLAIPRQLVLDDLDEPVSKAFDRAIGRLSAAGVRIEHVDMPELTELASLNAAGGFSAPESYAHHRALLAIHRDLYD
PRVVTRIERGAAMSAADYIALHRARLDWIARMEARMAPFDAVVCPTVPMVAPPIAPLVEDDTLFFRTNGLLLRNTSAFNF
LDGCSVSLPCHAPDELPVGLMLSQGPMRDAQLLGTALALESLVHPSRRGG
>Mature_450_residues
MLPDIASLNAAVRAGTTSRADLLAEAATKATSAAGHAVYLHTTFDAAIEVARAADAADRAGKPLHPLAGLPVSVKDLYDI
AGEVTRAASAARQDAAPATADATVVARLRAAGAALIGRTNMTEFAFSGVGINPHFGTPVNPCDSSVARIPGGSSSGAAVS
VAIGTAVAGLGSDTGGSIRIPAALCGIVGFKPTSRRVPLTGAFPLSYTLDTACAMARTLTDCIAVDSVIADVPFIPQMKA
STAIHLAIPRQLVLDDLDEPVSKAFDRAIGRLSAAGVRIEHVDMPELTELASLNAAGGFSAPESYAHHRALLAIHRDLYD
PRVVTRIERGAAMSAADYIALHRARLDWIARMEARMAPFDAVVCPTVPMVAPPIAPLVEDDTLFFRTNGLLLRNTSAFNF
LDGCSVSLPCHAPDELPVGLMLSQGPMRDAQLLGTALALESLVHPSRRGG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=24.748490945674, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI195972892, Length=214, Percent_Identity=36.4485981308411, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI166795287, Length=135, Percent_Identity=34.8148148148148, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=454, Percent_Identity=29.0748898678414, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71990152, Length=200, Percent_Identity=33.5, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=178, Percent_Identity=35.3932584269663, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=155, Percent_Identity=36.1290322580645, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=169, Percent_Identity=34.9112426035503, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=198, Percent_Identity=34.3434343434343, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=198, Percent_Identity=34.3434343434343, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=393, Percent_Identity=30.5343511450382, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6323950, Length=121, Percent_Identity=40.495867768595, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648113, Length=303, Percent_Identity=31.023102310231, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356731, Length=257, Percent_Identity=30.3501945525292, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=30.6451612903226, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648435, Length=247, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648437, Length=247, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648441, Length=221, Percent_Identity=31.2217194570136, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=221, Percent_Identity=31.2217194570136, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644968, Length=215, Percent_Identity=31.1627906976744, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652985, Length=172, Percent_Identity=33.1395348837209, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922090, Length=172, Percent_Identity=33.1395348837209, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24652981, Length=172, Percent_Identity=33.1395348837209, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24652983, Length=172, Percent_Identity=33.1395348837209, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI161078093, Length=248, Percent_Identity=29.4354838709677, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 46654; Mature: 46654

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPDIASLNAAVRAGTTSRADLLAEAATKATSAAGHAVYLHTTFDAAIEVARAADAADRA
CCCCCHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHC
GKPLHPLAGLPVSVKDLYDIAGEVTRAASAARQDAAPATADATVVARLRAAGAALIGRTN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHCCCCC
MTEFAFSGVGINPHFGTPVNPCDSSVARIPGGSSSGAAVSVAIGTAVAGLGSDTGGSIRI
CHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCEEC
PAALCGIVGFKPTSRRVPLTGAFPLSYTLDTACAMARTLTDCIAVDSVIADVPFIPQMKA
HHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
STAIHLAIPRQLVLDDLDEPVSKAFDRAIGRLSAAGVRIEHVDMPELTELASLNAAGGFS
CCEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCCCCCCC
APESYAHHRALLAIHRDLYDPRVVTRIERGAAMSAADYIALHRARLDWIARMEARMAPFD
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVVCPTVPMVAPPIAPLVEDDTLFFRTNGLLLRNTSAFNFLDGCSVSLPCHAPDELPVGL
EEECCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCEECCCCCCCCCCEEE
MLSQGPMRDAQLLGTALALESLVHPSRRGG
EECCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLPDIASLNAAVRAGTTSRADLLAEAATKATSAAGHAVYLHTTFDAAIEVARAADAADRA
CCCCCHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHC
GKPLHPLAGLPVSVKDLYDIAGEVTRAASAARQDAAPATADATVVARLRAAGAALIGRTN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHCCCCC
MTEFAFSGVGINPHFGTPVNPCDSSVARIPGGSSSGAAVSVAIGTAVAGLGSDTGGSIRI
CHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCEEC
PAALCGIVGFKPTSRRVPLTGAFPLSYTLDTACAMARTLTDCIAVDSVIADVPFIPQMKA
HHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
STAIHLAIPRQLVLDDLDEPVSKAFDRAIGRLSAAGVRIEHVDMPELTELASLNAAGGFS
CCEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCCCCCCC
APESYAHHRALLAIHRDLYDPRVVTRIERGAAMSAADYIALHRARLDWIARMEARMAPFD
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVVCPTVPMVAPPIAPLVEDDTLFFRTNGLLLRNTSAFNFLDGCSVSLPCHAPDELPVGL
EEECCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCEECCCCCCCCCCEEE
MLSQGPMRDAQLLGTALALESLVHPSRRGG
EECCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA