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The map label for this gene is rfaD [C]

Identifier: 94310778

GI number: 94310778

Start: 1994671

End: 1995642

Strand: Direct

Name: rfaD [C]

Synonym: Rmet_1840

Alternate gene names: 94310778

Gene position: 1994671-1995642 (Clockwise)

Preceding gene: 94310777

Following gene: 94310782

Centisome position: 50.78

GC content: 66.87

Gene sequence:

>972_bases
ATGAACGTACTGATTACCGGCGGCGCCGGCTTCCTGGGCCTGCAACTGGCCCGTCTGCTGCTCAAACGCGGCACGCTGAA
CCTGGATGGCAAGGCTGTCACGATCGACCGGCTCACGCTGCTCGACGTGGTGGCGCCGCAGATTGACGATGCCCGCGTGC
GCGCGGTAACGGGCGACCTGTCCGATCCCGCCGTGCTGGCGCAGGCCATCGACCGCGATACCGGCGCAATCTTCCATCTG
GCCGCCGTAGTCAGCGGTCAGGCCGAGGCCGATTTCGATCTGGGAATGCGCGTGAACCTTGACGCCTCGCGCGCGCTGCT
AGAGACGTGCCGCCAGTTGGCGCATGCGCCGCGTGTGGTGTTCACCAGCTCGGTCGCGGTCTATGGCGGCAAGCTGCCCC
CGGTGGTGCAGGACGACACCGCGCTGAATCCGCAATCCTCGTACGGCGTGCAGAAGGCCATCGGCGAATTGCTGCTGTCC
GACTACAGCCGACGCGGTTTTGTCGATGGCCGCGTGCTGCGGCTGCCGACGATCAGCGTGCGACCCGGTAAGCCGAACGC
CGCGGCGTCGTCGTTCGCCAGCGGCATCATCCGCGAACCGTTGTCGGGCGTGGCGGCAAATTGCCCTGTAGCACCGGAAA
CGCCGCTGTGGCTGCTCTCGCCGCGCGGCGCGATCCAGGCGCTGGTCAACGGCATTGAACTCGATGGCGCGCGCATCGGC
AATCGTCGGGTGGTGAATCTGCCGGGACTGTCGGTCACAGCGGCACAGATGGTGGAAGCACTGCGGCGCGTGGCCGGTGA
CGCGGTGGCCGATCGCGTGACGTGGGAACGCGAAGAGCGCATCGAGAAGATCGTCGGCACGTGGCCAGCGGCGTGGGATG
TGTCGCGCGCGCGCGAACTGGGTTTCGAGAGCGACGCGAGCTTTGACGACGTGATTCGGGCTTATGCGGAGGATGCGGCG
GTCAAGCTCTGA

Upstream 100 bases:

>100_bases
GGTCTGGGCTGGATCAAGCCCTGGCTGAAGCGCTGATCCGGGCCTGTCACGGCCGTTTCCGCTCCGTACCGACCACGCCA
ATGATGAACACTCCGCAACC

Downstream 100 bases:

>100_bases
TCCGGTGAGGTCGCGGTTGACAGGATTGACAGGCTTGCCCTCTGCCGCTCGCGGGAGAGGGCAAGCACATCAAACATCAT
CCCAACTCACGCAGCAGGCT

Product: nucleoside-diphosphate-sugar epimerase

Products: ADP-L-glycero-D-manno-heptose [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNVLITGGAGFLGLQLARLLLKRGTLNLDGKAVTIDRLTLLDVVAPQIDDARVRAVTGDLSDPAVLAQAIDRDTGAIFHL
AAVVSGQAEADFDLGMRVNLDASRALLETCRQLAHAPRVVFTSSVAVYGGKLPPVVQDDTALNPQSSYGVQKAIGELLLS
DYSRRGFVDGRVLRLPTISVRPGKPNAAASSFASGIIREPLSGVAANCPVAPETPLWLLSPRGAIQALVNGIELDGARIG
NRRVVNLPGLSVTAAQMVEALRRVAGDAVADRVTWEREERIEKIVGTWPAAWDVSRARELGFESDASFDDVIRAYAEDAA
VKL

Sequences:

>Translated_323_residues
MNVLITGGAGFLGLQLARLLLKRGTLNLDGKAVTIDRLTLLDVVAPQIDDARVRAVTGDLSDPAVLAQAIDRDTGAIFHL
AAVVSGQAEADFDLGMRVNLDASRALLETCRQLAHAPRVVFTSSVAVYGGKLPPVVQDDTALNPQSSYGVQKAIGELLLS
DYSRRGFVDGRVLRLPTISVRPGKPNAAASSFASGIIREPLSGVAANCPVAPETPLWLLSPRGAIQALVNGIELDGARIG
NRRVVNLPGLSVTAAQMVEALRRVAGDAVADRVTWEREERIEKIVGTWPAAWDVSRARELGFESDASFDDVIRAYAEDAA
VKL
>Mature_323_residues
MNVLITGGAGFLGLQLARLLLKRGTLNLDGKAVTIDRLTLLDVVAPQIDDARVRAVTGDLSDPAVLAQAIDRDTGAIFHL
AAVVSGQAEADFDLGMRVNLDASRALLETCRQLAHAPRVVFTSSVAVYGGKLPPVVQDDTALNPQSSYGVQKAIGELLLS
DYSRRGFVDGRVLRLPTISVRPGKPNAAASSFASGIIREPLSGVAANCPVAPETPLWLLSPRGAIQALVNGIELDGARIG
NRRVVNLPGLSVTAAQMVEALRRVAGDAVADRVTWEREERIEKIVGTWPAAWDVSRARELGFESDASFDDVIRAYAEDAA
VKL

Specific function: Biosynthesis Of The Lipopolysaccharide Precursor ADP-L- Glycerol-D-Mannoheptose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI32566934, Length=313, Percent_Identity=23.961661341853, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24667531, Length=303, Percent_Identity=26.4026402640264, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.20 [C]

Molecular weight: Translated: 34347; Mature: 34347

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLITGGAGFLGLQLARLLLKRGTLNLDGKAVTIDRLTLLDVVAPQIDDARVRAVTGDL
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCCEEEEEECCC
SDPAVLAQAIDRDTGAIFHLAAVVSGQAEADFDLGMRVNLDASRALLETCRQLAHAPRVV
CCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEE
FTSSVAVYGGKLPPVVQDDTALNPQSSYGVQKAIGELLLSDYSRRGFVDGRVLRLPTISV
EECCHHEECCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCEEEE
RPGKPNAAASSFASGIIREPLSGVAANCPVAPETPLWLLSPRGAIQALVNGIELDGARIG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCEECC
NRRVVNLPGLSVTAAQMVEALRRVAGDAVADRVTWEREERIEKIVGTWPAAWDVSRAREL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GFESDASFDDVIRAYAEDAAVKL
CCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNVLITGGAGFLGLQLARLLLKRGTLNLDGKAVTIDRLTLLDVVAPQIDDARVRAVTGDL
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCCEEEEEECCC
SDPAVLAQAIDRDTGAIFHLAAVVSGQAEADFDLGMRVNLDASRALLETCRQLAHAPRVV
CCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEE
FTSSVAVYGGKLPPVVQDDTALNPQSSYGVQKAIGELLLSDYSRRGFVDGRVLRLPTISV
EECCHHEECCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCEEEE
RPGKPNAAASSFASGIIREPLSGVAANCPVAPETPLWLLSPRGAIQALVNGIELDGARIG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHCCCCCCCEECC
NRRVVNLPGLSVTAAQMVEALRRVAGDAVADRVTWEREERIEKIVGTWPAAWDVSRAREL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GFESDASFDDVIRAYAEDAAVKL
CCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.1 {ADP-D-glycero-D-manno-heptose}} [C]

Substrates: ADP-D-glycero-D-manno-heptose [C]

Specific reaction: ADP-D-glycero-D-manno-heptose --> ADP-L-glycero-D-manno-heptose [C]

General reaction: Epimerization [C]

Inhibitor: ADP; ADP glucose; ATP; NADH [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]