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The map label for this gene is ygbJ [H]

Identifier: 94310773

GI number: 94310773

Start: 1989435

End: 1990331

Strand: Direct

Name: ygbJ [H]

Synonym: Rmet_1835

Alternate gene names: 94310773

Gene position: 1989435-1990331 (Clockwise)

Preceding gene: 94310772

Following gene: 94310774

Centisome position: 50.65

GC content: 67.34

Gene sequence:

>897_bases
ATGTCCAGGAATATTGGCGTCATCGGCTTGGGCGCGATGGGCTACGGCGTGGCGCAGTCGCTGCTGCGCGCCGGCTTCAA
CGTTCATGCATTTGATTTGCGTGACGAGGTGCTGCGCCGCTTCGCGGAAGCCGGTGGCACGCCCTGCGGGAGCCCGGCCG
AACTCGGCGAGCGTTGCGACGTGGTGATCACGCTCGTCGTCAACGCCGCGCAGACGGAAACCGTGCTGTTCGGCGACCAA
GGCGCCGCGTCGAAAATGGCGGCAGGCAAGCTCGTCATTGCCAGCGCCACCGTGCCGCCCGGATTTGCCGAAGCGCTCGG
CAAGCGCCTGGGCGAACGTGGCCTGCTGATGCTCGATGCCCCCGTGTCGGGTGGCGCGGCGCGCGCGGCCAGCGGCGAGA
TGACGATGATGACCTCCGGTCCCGCCGAGGCCTACGCCATGGCCGAGGACGTGCTGGCCGCGATGGCCGGCAAGGTCTAT
CGCCTGGGCGACGCGCATGGCGCGGGCTCGAAGGTCAAGATCATCAACCAGTTGCTGGCGGGCGTGCATATCGCCGCCGC
AGCCGAAGCGATGGCCCTGGGCCTGCGCGAAGGCGTGAACCCCGACGCGCTCTACGACGTGATCACGCATAGCGCCGGCA
ACTCGTGGATGTTCGAGAACCGGGTGCCACACATCCTGGAAGGCGACTACACCCCGCTGTCCGCAGTGGACATCTTCGTC
AAGGACCTCGGCATGGTGCTTGATACCGCGCGCACCAGCAAATTCCCGCTGCCGCTCTCGGCCGCCGCGCACCAGATGTT
CATGATGGCGTCCACCGCCGGGCACGGCGGCGAAGACGATTCGGCCGTGATCAAGATCTTCCCGGGCATCGACCTGCCCG
GCCCGAAGGCCAGGTAA

Upstream 100 bases:

>100_bases
ATTCCGCCGCTGGCCACCAGCCGGCAGGCCCGTCGCACTAACGCAGCAATACGCACCAAAACGCACCAAAACGCACCAAA
CACGCATTCAGGAGTTATCC

Downstream 100 bases:

>100_bases
CCCAAGGCAAAGGAGTCAGACGATGAATCGTCCTCTGCTCGGCTGTATCGCCGACGATTTCACCGGCGCCACGGATCTGG
CCAACACGCTAGTCCGCAAT

Product: putative dehydrogenase, with NAD(P)-binding Rossmann-fold domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSRNIGVIGLGAMGYGVAQSLLRAGFNVHAFDLRDEVLRRFAEAGGTPCGSPAELGERCDVVITLVVNAAQTETVLFGDQ
GAASKMAAGKLVIASATVPPGFAEALGKRLGERGLLMLDAPVSGGAARAASGEMTMMTSGPAEAYAMAEDVLAAMAGKVY
RLGDAHGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVNPDALYDVITHSAGNSWMFENRVPHILEGDYTPLSAVDIFV
KDLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPGPKAR

Sequences:

>Translated_298_residues
MSRNIGVIGLGAMGYGVAQSLLRAGFNVHAFDLRDEVLRRFAEAGGTPCGSPAELGERCDVVITLVVNAAQTETVLFGDQ
GAASKMAAGKLVIASATVPPGFAEALGKRLGERGLLMLDAPVSGGAARAASGEMTMMTSGPAEAYAMAEDVLAAMAGKVY
RLGDAHGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVNPDALYDVITHSAGNSWMFENRVPHILEGDYTPLSAVDIFV
KDLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPGPKAR
>Mature_297_residues
SRNIGVIGLGAMGYGVAQSLLRAGFNVHAFDLRDEVLRRFAEAGGTPCGSPAELGERCDVVITLVVNAAQTETVLFGDQG
AASKMAAGKLVIASATVPPGFAEALGKRLGERGLLMLDAPVSGGAARAASGEMTMMTSGPAEAYAMAEDVLAAMAGKVYR
LGDAHGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVNPDALYDVITHSAGNSWMFENRVPHILEGDYTPLSAVDIFVK
DLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPGPKAR

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=290, Percent_Identity=32.4137931034483, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=25.2631578947368, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI1789092, Length=297, Percent_Identity=54.5454545454545, Blast_Score=284, Evalue=4e-78,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=32.1428571428571, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1790315, Length=282, Percent_Identity=31.9148936170213, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=26.4084507042254, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557316, Length=270, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=30.0341296928328, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=30.0341296928328, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=24.0282685512367, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 30735; Mature: 30604

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNIGVIGLGAMGYGVAQSLLRAGFNVHAFDLRDEVLRRFAEAGGTPCGSPAELGERCD
CCCCCCEEEECHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCHHHHHCCCC
VVITLVVNAAQTETVLFGDQGAASKMAAGKLVIASATVPPGFAEALGKRLGERGLLMLDA
EEEEEEEECCCCEEEEECCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEC
PVSGGAARAASGEMTMMTSGPAEAYAMAEDVLAAMAGKVYRLGDAHGAGSKVKIINQLLA
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHH
GVHIAAAAEAMALGLREGVNPDALYDVITHSAGNSWMFENRVPHILEGDYTPLSAVDIFV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCHHCCCCCCCHHHHHHHH
KDLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPGPKAR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SRNIGVIGLGAMGYGVAQSLLRAGFNVHAFDLRDEVLRRFAEAGGTPCGSPAELGERCD
CCCCCEEEECHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCHHHHHCCCC
VVITLVVNAAQTETVLFGDQGAASKMAAGKLVIASATVPPGFAEALGKRLGERGLLMLDA
EEEEEEEECCCCEEEEECCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEC
PVSGGAARAASGEMTMMTSGPAEAYAMAEDVLAAMAGKVYRLGDAHGAGSKVKIINQLLA
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHH
GVHIAAAAEAMALGLREGVNPDALYDVITHSAGNSWMFENRVPHILEGDYTPLSAVDIFV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCHHCCCCCCCHHHHHHHH
KDLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPGPKAR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]