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The map label for this gene is oprM [H]

Identifier: 94310675

GI number: 94310675

Start: 1881726

End: 1883219

Strand: Reverse

Name: oprM [H]

Synonym: Rmet_1737

Alternate gene names: 94310675

Gene position: 1883219-1881726 (Counterclockwise)

Preceding gene: 94310676

Following gene: 94310673

Centisome position: 47.94

GC content: 65.06

Gene sequence:

>1494_bases
ATGAAAAGAAAGCCAACCTCCCTGATTCGCCCGCCGCGCTGGACCCCTGCCCTCCTCGTGCCGCTGGCGCTTGCCGCCTG
CTCGCTGGAACCCACCTACCAACGTCCGGAGTCACCGGTACCGCAGGCTTACCCGACCGGCCCTGCCTATGAAAGCGCCA
ACAAGGGGCCCGCGGGCCAGGCGCGGCCGGACCAGGCAACGCCCGCCGCGAGCCTTGGCTGGGAGGACTTCTTTACCGAC
CCACGCCTGCGCACGCTGATCGGGCTGGCGCTGGCGAACAACCGTGACCTGCGTATCGCGACGCTGTCCATCGATCAGGC
GCGAGCGCAGTACCGGATCCAGCGTGCCGCGCAGTTTCCGGCGATCAACGCCAATGCAGGCTTTGCCAGTACTCGGCTGA
ACAGCGATCTGCGCGCGCCCGGACAGGACGCCGTGATCAACTCCTGGACTGCGGGCGTAGGCTTCAATGCATTTGAGCTC
GATGTGTTCGGGCGGGTGCGCAGCCTCAAGCATCAGGCGCTGGAGCAATATCTGGCAATCGAGGAAGTGCGGCGCAGCGC
GCAGATCAGTCTCGTGGCGGAAGTGGCCAGCGCCTACCTGACATGGCAGGCCGACCAGGAACTGCTCAGGCTGTCGAAGG
AAACGCTCAAGGTCCAGCAGGACGCCGCGCTGATGATCAGCCGCAGCAAGAGCGCCGGCGGCATGGCGGAGATCGACATG
CATCGCGGGCAGACCCAGGTGCAGACCGCGCAGGTGGATGTGGAGCAGTTCACGCGACAGGTGGCGCAGGACGAGAATGC
GCTGGCGTTGTTGATCGGCGGCCAGCTACCGGCGGACCTGCCGCCCCCGCAGCCGTTTGCGAATAACTCGTACGTGGCAG
AGTTGCCCGCCGGGGTGCCATCGACACTGATGGAGCAACGGCCAGACATCATGGCGGCCGAGCATCAGCTCAAGGGGGCC
AATGCCAACATCGGCGCGGCGCGGGCGGCCTTCTTCCCGCAGATATCGCTGACGGCCACCGTCGGCGTGGCCAGCACGGC
GCTCGCCTCGCTGTTCACGGGCGGCATGGCCTGGGCGTTCGCGCCGCAGATCACACTGCCGATTTTTAACGCCGGGGCCA
ACCAGGCACGACTCGATATTGCCACGGTGCAGAAGGACATTAACGTGGCGGCTTACGAGAAAACGATCCAGGGTGCATTC
CGGGAAGTTGCCGATGGCCTGGCTGCACGCGGCACCTACGACCGGCAGACCGTGGCGCAGGAGGCGCTGACGAAGGAAGT
TTCCGAAACCAAGCGGCTGGCGGAAATCCGTTTCAAGAACGGGGTGGATGACTACTTCCCCGTGTTCGATGCGCAGCGGC
AACTCTACGATGCGCAGAAGAAGATGGTGACGATCGAACTGGCCAGACTCACCAGCCGCGCAAGCCTATACAAGGCGCTG
GGCGGCGGGTGGTCGCAGCAGACGGTGGCCAGCCTAGATCATCCGGCGAAGTAG

Upstream 100 bases:

>100_bases
CCGGATGCTGTTCGGCGAACCGGCTCACAACATCGCGCACCGCGGCCGCCCGCGTGTGGCCATCGTGCAATTGCTGCGTC
GCGCATTTACCGGATGAACC

Downstream 100 bases:

>100_bases
CCGGTCCTTCAGGACGAACTGGTGCCACAAGGCGGCTGCGGCGTGCAGGCCGACGAAGATGTACACGAACGTGGCGCCAG
TTTCGTGAATCTCCTTCGCG

Product: outer membrane efflux protein, putative copper/silver efflux system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKRKPTSLIRPPRWTPALLVPLALAACSLEPTYQRPESPVPQAYPTGPAYESANKGPAGQARPDQATPAASLGWEDFFTD
PRLRTLIGLALANNRDLRIATLSIDQARAQYRIQRAAQFPAINANAGFASTRLNSDLRAPGQDAVINSWTAGVGFNAFEL
DVFGRVRSLKHQALEQYLAIEEVRRSAQISLVAEVASAYLTWQADQELLRLSKETLKVQQDAALMISRSKSAGGMAEIDM
HRGQTQVQTAQVDVEQFTRQVAQDENALALLIGGQLPADLPPPQPFANNSYVAELPAGVPSTLMEQRPDIMAAEHQLKGA
NANIGAARAAFFPQISLTATVGVASTALASLFTGGMAWAFAPQITLPIFNAGANQARLDIATVQKDINVAAYEKTIQGAF
REVADGLAARGTYDRQTVAQEALTKEVSETKRLAEIRFKNGVDDYFPVFDAQRQLYDAQKKMVTIELARLTSRASLYKAL
GGGWSQQTVASLDHPAK

Sequences:

>Translated_497_residues
MKRKPTSLIRPPRWTPALLVPLALAACSLEPTYQRPESPVPQAYPTGPAYESANKGPAGQARPDQATPAASLGWEDFFTD
PRLRTLIGLALANNRDLRIATLSIDQARAQYRIQRAAQFPAINANAGFASTRLNSDLRAPGQDAVINSWTAGVGFNAFEL
DVFGRVRSLKHQALEQYLAIEEVRRSAQISLVAEVASAYLTWQADQELLRLSKETLKVQQDAALMISRSKSAGGMAEIDM
HRGQTQVQTAQVDVEQFTRQVAQDENALALLIGGQLPADLPPPQPFANNSYVAELPAGVPSTLMEQRPDIMAAEHQLKGA
NANIGAARAAFFPQISLTATVGVASTALASLFTGGMAWAFAPQITLPIFNAGANQARLDIATVQKDINVAAYEKTIQGAF
REVADGLAARGTYDRQTVAQEALTKEVSETKRLAEIRFKNGVDDYFPVFDAQRQLYDAQKKMVTIELARLTSRASLYKAL
GGGWSQQTVASLDHPAK
>Mature_497_residues
MKRKPTSLIRPPRWTPALLVPLALAACSLEPTYQRPESPVPQAYPTGPAYESANKGPAGQARPDQATPAASLGWEDFFTD
PRLRTLIGLALANNRDLRIATLSIDQARAQYRIQRAAQFPAINANAGFASTRLNSDLRAPGQDAVINSWTAGVGFNAFEL
DVFGRVRSLKHQALEQYLAIEEVRRSAQISLVAEVASAYLTWQADQELLRLSKETLKVQQDAALMISRSKSAGGMAEIDM
HRGQTQVQTAQVDVEQFTRQVAQDENALALLIGGQLPADLPPPQPFANNSYVAELPAGVPSTLMEQRPDIMAAEHQLKGA
NANIGAARAAFFPQISLTATVGVASTALASLFTGGMAWAFAPQITLPIFNAGANQARLDIATVQKDINVAAYEKTIQGAF
REVADGLAARGTYDRQTVAQEALTKEVSETKRLAEIRFKNGVDDYFPVFDAQRQLYDAQKKMVTIELARLTSRASLYKAL
GGGWSQQTVASLDHPAK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=478, Percent_Identity=39.1213389121339, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI2367348, Length=463, Percent_Identity=25.7019438444924, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53883; Mature: 53883

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKPTSLIRPPRWTPALLVPLALAACSLEPTYQRPESPVPQAYPTGPAYESANKGPAGQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ARPDQATPAASLGWEDFFTDPRLRTLIGLALANNRDLRIATLSIDQARAQYRIQRAAQFP
CCCCCCCCCHHCCCHHHCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCC
AINANAGFASTRLNSDLRAPGQDAVINSWTAGVGFNAFELDVFGRVRSLKHQALEQYLAI
CCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
EEVRRSAQISLVAEVASAYLTWQADQELLRLSKETLKVQQDAALMISRSKSAGGMAEIDM
HHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCCCEEEHH
HRGQTQVQTAQVDVEQFTRQVAQDENALALLIGGQLPADLPPPQPFANNSYVAELPAGVP
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCC
STLMEQRPDIMAAEHQLKGANANIGAARAAFFPQISLTATVGVASTALASLFTGGMAWAF
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCHHEEE
APQITLPIFNAGANQARLDIATVQKDINVAAYEKTIQGAFREVADGLAARGTYDRQTVAQ
CCEEEEEEECCCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EALTKEVSETKRLAEIRFKNGVDDYFPVFDAQRQLYDAQKKMVTIELARLTSRASLYKAL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHH
GGGWSQQTVASLDHPAK
CCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MKRKPTSLIRPPRWTPALLVPLALAACSLEPTYQRPESPVPQAYPTGPAYESANKGPAGQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ARPDQATPAASLGWEDFFTDPRLRTLIGLALANNRDLRIATLSIDQARAQYRIQRAAQFP
CCCCCCCCCHHCCCHHHCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCC
AINANAGFASTRLNSDLRAPGQDAVINSWTAGVGFNAFELDVFGRVRSLKHQALEQYLAI
CCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
EEVRRSAQISLVAEVASAYLTWQADQELLRLSKETLKVQQDAALMISRSKSAGGMAEIDM
HHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCCCEEEHH
HRGQTQVQTAQVDVEQFTRQVAQDENALALLIGGQLPADLPPPQPFANNSYVAELPAGVP
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCC
STLMEQRPDIMAAEHQLKGANANIGAARAAFFPQISLTATVGVASTALASLFTGGMAWAF
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCHHEEE
APQITLPIFNAGANQARLDIATVQKDINVAAYEKTIQGAFREVADGLAARGTYDRQTVAQ
CCEEEEEEECCCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EALTKEVSETKRLAEIRFKNGVDDYFPVFDAQRQLYDAQKKMVTIELARLTSRASLYKAL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHH
GGGWSQQTVASLDHPAK
CCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]