Click here to switch to the map view.

The map label for this gene is mdtN [H]

Identifier: 94310651

GI number: 94310651

Start: 1853423

End: 1854559

Strand: Direct

Name: mdtN [H]

Synonym: Rmet_1711

Alternate gene names: 94310651

Gene position: 1853423-1854559 (Clockwise)

Preceding gene: 94310650

Following gene: 94310652

Centisome position: 47.18

GC content: 67.28

Gene sequence:

>1137_bases
ATGAGTGACGCCAGTGATGCGAATGCGCCAGCCACGCCGCCTGCCTCGCCAGGCCCGGCGGCATCCGGAGACGCGTCGGG
AAAAGGGGTGCGGTGGGTTGTCATCGCGATCGTGCTCAGTCTGGTCTGGTATCTGCTGGCCGACCGCTTCACGCCTTATA
CCCAGCAGGCGCGTGTGCAGGCGTTCGTGATTCCAGTGGCTTCAGAAGTGTCTGGCCGCGTCACCCAGGTGCCGGTTCAC
AACAACCAGAATGTGGAGGTCGGCACCGTGCTGTTCGAGGTCGACCCACAGCAGTACCGGATCGCGCTGGACCGCGCCCG
CGCCGATTTCGAATCCACGCGCCGGCAGATCGGCGCGAACACGGCGGGCATCGATTCCGCGCGCGAATCGCTGCGAGCGG
CGCAGGCCAACGAGCTCAAGGCGCGACAGGACAGCGAGCGGCTTGAGCGTCTGTATCGGGAAGACCCCGGCACGGTTTCC
GTGCGGCGGCTGGAGGTGGCGCGCGCCACGCACACGCAGGCCGTCAGCCAGGTCGGCGCGGCCCGCGCCGAGGTCGAACG
GGCCAAGGAGCAGCAGGGCGGCAGCGAAGAGAACAACGCCAAGCTGCGCAGCGCCGCTGCGGCGGTTGAAAAGGCGGAGC
TCGATCTGTCCAATGCCAAGATCCGGGCGCGTACCGGCGGGCTGGTCACCGACCTGCAAACGGAAGTCGGCCGTTTTGCC
GGCGCCGGCAGCCCGGTCATGACGCTGATCGCGATCCAGGATATCTGGATTACCGCCGACCTCACCGAAAACAACCTGGG
CCACGTAGAGCCGGGCACGCCCGTGGCCATCGTACTCGACGCGCTGCCGGGCAAGGTGCTCGAAGGCAAGGTGCGCAGCA
TTGGCTACGGCGTGAGCACCGGCCAGAGCCCGCCACCAGGCAGCCTGCCGACGGTGCAGAACAGCCGCGACTGGCTGCGG
CCCGCGCAACGCTTTCCGGTGATCGTCGATATCGATCCGGGGCAACTCTCTTCGCTGCGCGGCGTGCGCGTGGGCGGCCA
GGCCGAAGTGATGGCGTTCCCGACCTCAGGCAACCCGCTCAATCCGTTGGGCCGGCTGTACATCCGCCTGATGAGCTGGC
TGTCCTATGTCTACTGA

Upstream 100 bases:

>100_bases
TGCGGCAGCGTACCGATTGGGGAGACCTGCTGGACGAGCCGCCGCGCACGCCGCCGACATCCAATAACACCACGGCCAAA
GCCACCGGGAGCCAACGCCA

Downstream 100 bases:

>100_bases
CGCAGGCGCATCGGCCGCCGCCACTGCCGCAACCGCGGCGCGGTCCCGGCGCGCGTTGCGCCTGGCTGTCGGCACCGCGC
TCTGTCTGGCGACCAGCTTC

Product: multidrug resistance efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSDASDANAPATPPASPGPAASGDASGKGVRWVVIAIVLSLVWYLLADRFTPYTQQARVQAFVIPVASEVSGRVTQVPVH
NNQNVEVGTVLFEVDPQQYRIALDRARADFESTRRQIGANTAGIDSARESLRAAQANELKARQDSERLERLYREDPGTVS
VRRLEVARATHTQAVSQVGAARAEVERAKEQQGGSEENNAKLRSAAAAVEKAELDLSNAKIRARTGGLVTDLQTEVGRFA
GAGSPVMTLIAIQDIWITADLTENNLGHVEPGTPVAIVLDALPGKVLEGKVRSIGYGVSTGQSPPPGSLPTVQNSRDWLR
PAQRFPVIVDIDPGQLSSLRGVRVGGQAEVMAFPTSGNPLNPLGRLYIRLMSWLSYVY

Sequences:

>Translated_378_residues
MSDASDANAPATPPASPGPAASGDASGKGVRWVVIAIVLSLVWYLLADRFTPYTQQARVQAFVIPVASEVSGRVTQVPVH
NNQNVEVGTVLFEVDPQQYRIALDRARADFESTRRQIGANTAGIDSARESLRAAQANELKARQDSERLERLYREDPGTVS
VRRLEVARATHTQAVSQVGAARAEVERAKEQQGGSEENNAKLRSAAAAVEKAELDLSNAKIRARTGGLVTDLQTEVGRFA
GAGSPVMTLIAIQDIWITADLTENNLGHVEPGTPVAIVLDALPGKVLEGKVRSIGYGVSTGQSPPPGSLPTVQNSRDWLR
PAQRFPVIVDIDPGQLSSLRGVRVGGQAEVMAFPTSGNPLNPLGRLYIRLMSWLSYVY
>Mature_377_residues
SDASDANAPATPPASPGPAASGDASGKGVRWVVIAIVLSLVWYLLADRFTPYTQQARVQAFVIPVASEVSGRVTQVPVHN
NQNVEVGTVLFEVDPQQYRIALDRARADFESTRRQIGANTAGIDSARESLRAAQANELKARQDSERLERLYREDPGTVSV
RRLEVARATHTQAVSQVGAARAEVERAKEQQGGSEENNAKLRSAAAAVEKAELDLSNAKIRARTGGLVTDLQTEVGRFAG
AGSPVMTLIAIQDIWITADLTENNLGHVEPGTPVAIVLDALPGKVLEGKVRSIGYGVSTGQSPPPGSLPTVQNSRDWLRP
AQRFPVIVDIDPGQLSSLRGVRVGGQAEVMAFPTSGNPLNPLGRLYIRLMSWLSYVY

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790519, Length=289, Percent_Identity=34.6020761245675, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1790012, Length=347, Percent_Identity=27.6657060518732, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790024, Length=346, Percent_Identity=28.6127167630058, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789637, Length=308, Percent_Identity=29.5454545454545, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081951, Length=314, Percent_Identity=25.796178343949, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789041, Length=310, Percent_Identity=26.7741935483871, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788711, Length=290, Percent_Identity=26.8965517241379, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40530; Mature: 40399

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDASDANAPATPPASPGPAASGDASGKGVRWVVIAIVLSLVWYLLADRFTPYTQQARVQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCEEE
AFVIPVASEVSGRVTQVPVHNNQNVEVGTVLFEVDPQQYRIALDRARADFESTRRQIGAN
EEEEEEHHCCCCCEEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
TAGIDSARESLRAAQANELKARQDSERLERLYREDPGTVSVRRLEVARATHTQAVSQVGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHH
ARAEVERAKEQQGGSEENNAKLRSAAAAVEKAELDLSNAKIRARTGGLVTDLQTEVGRFA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHC
GAGSPVMTLIAIQDIWITADLTENNLGHVEPGTPVAIVLDALPGKVLEGKVRSIGYGVST
CCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHCHHHHHCCCCCC
GQSPPPGSLPTVQNSRDWLRPAQRFPVIVDIDPGQLSSLRGVRVGGQAEVMAFPTSGNPL
CCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHCCCEEECCCEEEEEECCCCCCC
NPLGRLYIRLMSWLSYVY
CHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDASDANAPATPPASPGPAASGDASGKGVRWVVIAIVLSLVWYLLADRFTPYTQQARVQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCEEE
AFVIPVASEVSGRVTQVPVHNNQNVEVGTVLFEVDPQQYRIALDRARADFESTRRQIGAN
EEEEEEHHCCCCCEEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
TAGIDSARESLRAAQANELKARQDSERLERLYREDPGTVSVRRLEVARATHTQAVSQVGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHH
ARAEVERAKEQQGGSEENNAKLRSAAAAVEKAELDLSNAKIRARTGGLVTDLQTEVGRFA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHC
GAGSPVMTLIAIQDIWITADLTENNLGHVEPGTPVAIVLDALPGKVLEGKVRSIGYGVST
CCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHCHHHHHCCCCCC
GQSPPPGSLPTVQNSRDWLRPAQRFPVIVDIDPGQLSSLRGVRVGGQAEVMAFPTSGNPL
CCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHCCCEEECCCEEEEEECCCCCCC
NPLGRLYIRLMSWLSYVY
CHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]