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The map label for this gene is fixJ [H]

Identifier: 94310638

GI number: 94310638

Start: 1840342

End: 1840917

Strand: Direct

Name: fixJ [H]

Synonym: Rmet_1698

Alternate gene names: 94310638

Gene position: 1840342-1840917 (Clockwise)

Preceding gene: 94310637

Following gene: 94310639

Centisome position: 46.85

GC content: 63.37

Gene sequence:

>576_bases
GTGCGGCGTGCCCTGGCACGCGTCCTTGGCGCCCATCGGTATCACACCAGTTGCTTCGATTCCGCCAAGCGCTTTCTGGA
TAACGTCGAACTGGAGAATTCGCCGATCTGTGCCGTGGTCGACCTGCAGATGCCAGACATGGACGGCATCGCCTTGCAGC
AGACGCTGAGCCAGCGAGTTCCCGTCGTCATCCTCACCGGACATGCCGACGTGTCCAAGGCGGTACAGGCCATGCAGGCG
GGCGCGATAGATTTTCTCGAAAAGCCGGTGCAGGACGGGCTGCTTCTGGCTGCGGTCGAACGGGCGTGTGAACGCGCGCG
GGAACAGTTCGCGCGCAAGGCCGAACTCCGCGTGCTGCGGCAGAAGATCGAGCGGCTGACGCCCAGAGAGCGTGAAGTCC
TCGGCTGGATCGTGTCGGGCCTGCGCAACAAGCAGGTGGCCAGCGAACTCGGGACCGTGGAAAAGACCATCAAGGTGCAT
CGCGCGCACGTGATGCAAAAGCTCGAAGTGGACTCGCTTCCAGCCCTGGTCCGCATTGCCGACAAGATGGGCATGGGGAT
CGTCGCGCGCACCTGA

Upstream 100 bases:

>100_bases
TTGCCACTCGCACCGCGCGGCATCCATCCGCTCCATCACGGAGGAAAGAACGATGAACGCAATGCAGCCAACGGTATGTA
TCGTCGATGACGACGACAGT

Downstream 100 bases:

>100_bases
CCCTGCGCTGGCTCCCCCGTTTGGACCATCGTCCAATATCGCCGGACGCCGGTGTCTCGTAGCCTTGGGGAAGGCAACTT
CTTCCCCGCGGTCTACGCGG

Product: two component response regulator, LuxR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MRRALARVLGAHRYHTSCFDSAKRFLDNVELENSPICAVVDLQMPDMDGIALQQTLSQRVPVVILTGHADVSKAVQAMQA
GAIDFLEKPVQDGLLLAAVERACERAREQFARKAELRVLRQKIERLTPREREVLGWIVSGLRNKQVASELGTVEKTIKVH
RAHVMQKLEVDSLPALVRIADKMGMGIVART

Sequences:

>Translated_191_residues
MRRALARVLGAHRYHTSCFDSAKRFLDNVELENSPICAVVDLQMPDMDGIALQQTLSQRVPVVILTGHADVSKAVQAMQA
GAIDFLEKPVQDGLLLAAVERACERAREQFARKAELRVLRQKIERLTPREREVLGWIVSGLRNKQVASELGTVEKTIKVH
RAHVMQKLEVDSLPALVRIADKMGMGIVART
>Mature_191_residues
MRRALARVLGAHRYHTSCFDSAKRFLDNVELENSPICAVVDLQMPDMDGIALQQTLSQRVPVVILTGHADVSKAVQAMQA
GAIDFLEKPVQDGLLLAAVERACERAREQFARKAELRVLRQKIERLTPREREVLGWIVSGLRNKQVASELGTVEKTIKVH
RAHVMQKLEVDSLPALVRIADKMGMGIVART

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=154, Percent_Identity=30.5194805194805, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1790299, Length=112, Percent_Identity=35.7142857142857, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788550, Length=126, Percent_Identity=30.952380952381, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21298; Mature: 21298

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRALARVLGAHRYHTSCFDSAKRFLDNVELENSPICAVVDLQMPDMDGIALQQTLSQRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCC
PVVILTGHADVSKAVQAMQAGAIDFLEKPVQDGLLLAAVERACERAREQFARKAELRVLR
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKIERLTPREREVLGWIVSGLRNKQVASELGTVEKTIKVHRAHVMQKLEVDSLPALVRIA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DKMGMGIVART
HHHCCCCEECC
>Mature Secondary Structure
MRRALARVLGAHRYHTSCFDSAKRFLDNVELENSPICAVVDLQMPDMDGIALQQTLSQRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCC
PVVILTGHADVSKAVQAMQAGAIDFLEKPVQDGLLLAAVERACERAREQFARKAELRVLR
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKIERLTPREREVLGWIVSGLRNKQVASELGTVEKTIKVHRAHVMQKLEVDSLPALVRIA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DKMGMGIVART
HHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]