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The map label for this gene is todF [H]

Identifier: 94310508

GI number: 94310508

Start: 1687539

End: 1688372

Strand: Reverse

Name: todF [H]

Synonym: Rmet_1566

Alternate gene names: 94310508

Gene position: 1688372-1687539 (Counterclockwise)

Preceding gene: 94310509

Following gene: 94310507

Centisome position: 42.98

GC content: 63.31

Gene sequence:

>834_bases
ATGCAACGTCTGAATGTGGACGCGCCCGGCATTCCCAGAACCAGTGTGCTGGACGCGGGCCAGGGCAGAGCCACAATTCT
GATTCACGGCACGTCCTCAAGCGCCGAAATGGGTTGGGCGCAGTTGCTGCCCAGGCTGGCAGCCAAACGGCGCTGCCTCG
CGTTCGACATGGTCGGCGCAGGCCAGACCGAAGACCCGGGCGGCCCCATCGCCCTGACCACGCTGGTCGAGCAGGTCCGC
GCCGTCGCCGACCTTGCTGGAGACGGCAAGCCTCTGGATCTCATCGGCTATTCGCTGGGTGCCGTCGTCGCCGCGGTCGC
GGCCGCTGCAATGCCCGATCGTGTGCGCCGACTGATCTTGCTAGGTGGCTGGGTGCAGAGCAGCAGAAGCATGTGCGTGC
TTTTTGACCTCTGGGCGGAGCTGTCACGAACCGACAAGCACCAGCTTGCGAGACTGCTGCTGGTCAACGGCGTGTCCGAT
ACCTTCTTTCAAGCCAACTCCACTGAGGCGATACAGACCGCACTGGACCGCATCGCCAACCTGCTTTCTGCCGGAGGCGA
TCGCCAGGCCGAGCTCGATGCCACGATCGATATTCGCGCCGAGCTACCCAGGATAAGAGCCCAGACCCTGGTGATCGGTA
TGCAACAAGATCGACTGGTGCCGCCCGCGCATTGCCGCGATCTGGCTGCAGGCATAGCGGGCGCGCGTTACGAGCAGATC
GACTGCGGCCACCTCGTCACGCTGGAACAGCCCGGTGCCCTGCTCGATCTGGTTGAAAGTCATCTTGATAGTCCGACAGG
ACATTGCCACGAAGAACGACACGGTGGCGCATGA

Upstream 100 bases:

>100_bases
ACATCCGCGTAGGCATTAACGGCCAGCAAATCGAGCGTTGGCTGCAAGCGACCTGACGCAGTCTGGTCGATCCCTTTCTC
TTCCTCACTCAAGGACTCCT

Downstream 100 bases:

>100_bases
CGTCATCGGCGTTCTTCACGTGAGTGGCCAGGGCCACATACGATATCTCAGGAGACAACCCCATGAATGCCAACATCTTG
TCCGGGCGCCAGCCTGCCCG

Product: putative esterase/lipase/thioesterase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: HOHH [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MQRLNVDAPGIPRTSVLDAGQGRATILIHGTSSSAEMGWAQLLPRLAAKRRCLAFDMVGAGQTEDPGGPIALTTLVEQVR
AVADLAGDGKPLDLIGYSLGAVVAAVAAAAMPDRVRRLILLGGWVQSSRSMCVLFDLWAELSRTDKHQLARLLLVNGVSD
TFFQANSTEAIQTALDRIANLLSAGGDRQAELDATIDIRAELPRIRAQTLVIGMQQDRLVPPAHCRDLAAGIAGARYEQI
DCGHLVTLEQPGALLDLVESHLDSPTGHCHEERHGGA

Sequences:

>Translated_277_residues
MQRLNVDAPGIPRTSVLDAGQGRATILIHGTSSSAEMGWAQLLPRLAAKRRCLAFDMVGAGQTEDPGGPIALTTLVEQVR
AVADLAGDGKPLDLIGYSLGAVVAAVAAAAMPDRVRRLILLGGWVQSSRSMCVLFDLWAELSRTDKHQLARLLLVNGVSD
TFFQANSTEAIQTALDRIANLLSAGGDRQAELDATIDIRAELPRIRAQTLVIGMQQDRLVPPAHCRDLAAGIAGARYEQI
DCGHLVTLEQPGALLDLVESHLDSPTGHCHEERHGGA
>Mature_277_residues
MQRLNVDAPGIPRTSVLDAGQGRATILIHGTSSSAEMGWAQLLPRLAAKRRCLAFDMVGAGQTEDPGGPIALTTLVEQVR
AVADLAGDGKPLDLIGYSLGAVVAAVAAAAMPDRVRRLILLGGWVQSSRSMCVLFDLWAELSRTDKHQLARLLLVNGVSD
TFFQANSTEAIQTALDRIANLLSAGGDRQAELDATIDIRAELPRIRAQTLVIGMQQDRLVPPAHCRDLAAGIAGARYEQI
DCGHLVTLEQPGALLDLVESHLDSPTGHCHEERHGGA

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 29482; Mature: 29482

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLNVDAPGIPRTSVLDAGQGRATILIHGTSSSAEMGWAQLLPRLAAKRRCLAFDMVGA
CCCCCCCCCCCCCHHHEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCC
GQTEDPGGPIALTTLVEQVRAVADLAGDGKPLDLIGYSLGAVVAAVAAAAMPDRVRRLIL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LGGWVQSSRSMCVLFDLWAELSRTDKHQLARLLLVNGVSDTFFQANSTEAIQTALDRIAN
HCCHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHH
LLSAGGDRQAELDATIDIRAELPRIRAQTLVIGMQQDRLVPPAHCRDLAAGIAGARYEQI
HHHCCCCCCEEECCEEEEHHHCCHHHEHHEEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
DCGHLVTLEQPGALLDLVESHLDSPTGHCHEERHGGA
CCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQRLNVDAPGIPRTSVLDAGQGRATILIHGTSSSAEMGWAQLLPRLAAKRRCLAFDMVGA
CCCCCCCCCCCCCHHHEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCC
GQTEDPGGPIALTTLVEQVRAVADLAGDGKPLDLIGYSLGAVVAAVAAAAMPDRVRRLIL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LGGWVQSSRSMCVLFDLWAELSRTDKHQLARLLLVNGVSDTFFQANSTEAIQTALDRIAN
HCCHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHH
LLSAGGDRQAELDATIDIRAELPRIRAQTLVIGMQQDRLVPPAHCRDLAAGIAGARYEQI
HHHCCCCCCEEECCEEEEHHHCCHHHEHHEEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
DCGHLVTLEQPGALLDLVESHLDSPTGHCHEERHGGA
CCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916282; 2670929 [H]