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The map label for this gene is cysU [H]

Identifier: 94310318

GI number: 94310318

Start: 1485321

End: 1486169

Strand: Direct

Name: cysU [H]

Synonym: Rmet_1376

Alternate gene names: 94310318

Gene position: 1485321-1486169 (Clockwise)

Preceding gene: 94310317

Following gene: 94310319

Centisome position: 37.81

GC content: 65.14

Gene sequence:

>849_bases
GTGTTGCCGGGCTTTGGCCTGTCGCTCGGCTTCACGATCTTCTACCTGACGCTTATCGTCCTGATCCCGCTGTCGGCCAC
GTTCCTGAAGACGTTCACGATGACGTGGGACGCGTTCTGGACCACGGTTTCGAACCCGCGCGTGGTGGCGTCGCTGGAAC
TCAGCTTTGGTGCGTCGTTGGTGGCCGCGATCATCAACACGGTGTTTGGCCTGGTCGTGGCATGGGTGCTGGTGCGCTAT
CGGTTCTTTGGCAAACGGGTGATCGACGCGCTGGTGGACCTGCCGTTCGCGCTGCCCACTGCCGTGGCCGGTATCGCGCT
GACCGCGCTGTTCGCGGGCAACGGCTGGATTGGCCGCTGGCTGGAACCGCTTGGCATCAAGGTGGCCTTCACGCCGTTGG
GCGTGGTGGTGGCCCTGACCTTCATCGGCCTGCCGTTCGTGGTGCGCACGGTGCAACCGGTGCTCGAGGATGTCGAGCGC
GAACTGGAGGAGGCCGCCGCGAGCCTGGGCGCTTCCCGGCTGCAGACGTTCTCGAGGGTGATCCTGCCCGCCATCCTGCC
GGCGCTGCTGACGGGCTTCGCGCTGTCGTTCGCCCGTGCCACGGGCGAGTACGGATCGGTGGTGTTCATCTCCGGCAACA
TGCCGATGGTCTCCGAGATCGCGCCGCTGATGATCTACTCGAAGCTCGAACAGTACGACTACGCCGGTGCCACGGCCGTG
GCCGTGGTGATGCTGGTGATCTCCTTCGTGCTGCTGTTGCTGATCAACCTGTTGCAAGCCTGGACGCGCCGTCACCAGAG
CGGTGTCCGTGCCGCCGCGCTGCCCGATGGCAATCCGGAGGTGAACTGA

Upstream 100 bases:

>100_bases
AGACGAGACACGCATGCCTTCCACGATTTCCCTGGCGGACGCCCCCCCGCCGGCCGCCGAAGCACCAAGGCGGCCCGTAT
CCGCGAGCCAGCGCTTCACG

Downstream 100 bases:

>100_bases
GATGGCTGGTGCAATTTCCCGTCTGGGTGGCCCAGGTGCCCCAGGCGCCCCAAGTGCCCACGTACAGGCCGCGCAGTTCC
CGCACGACGCCACTGGCGAA

Product: sulfate/thiosulfate ABC transporter membrane protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLPGFGLSLGFTIFYLTLIVLIPLSATFLKTFTMTWDAFWTTVSNPRVVASLELSFGASLVAAIINTVFGLVVAWVLVRY
RFFGKRVIDALVDLPFALPTAVAGIALTALFAGNGWIGRWLEPLGIKVAFTPLGVVVALTFIGLPFVVRTVQPVLEDVER
ELEEAAASLGASRLQTFSRVILPAILPALLTGFALSFARATGEYGSVVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAV
AVVMLVISFVLLLLINLLQAWTRRHQSGVRAAALPDGNPEVN

Sequences:

>Translated_282_residues
MLPGFGLSLGFTIFYLTLIVLIPLSATFLKTFTMTWDAFWTTVSNPRVVASLELSFGASLVAAIINTVFGLVVAWVLVRY
RFFGKRVIDALVDLPFALPTAVAGIALTALFAGNGWIGRWLEPLGIKVAFTPLGVVVALTFIGLPFVVRTVQPVLEDVER
ELEEAAASLGASRLQTFSRVILPAILPALLTGFALSFARATGEYGSVVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAV
AVVMLVISFVLLLLINLLQAWTRRHQSGVRAAALPDGNPEVN
>Mature_282_residues
MLPGFGLSLGFTIFYLTLIVLIPLSATFLKTFTMTWDAFWTTVSNPRVVASLELSFGASLVAAIINTVFGLVVAWVLVRY
RFFGKRVIDALVDLPFALPTAVAGIALTALFAGNGWIGRWLEPLGIKVAFTPLGVVVALTFIGLPFVVRTVQPVLEDVER
ELEEAAASLGASRLQTFSRVILPAILPALLTGFALSFARATGEYGSVVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAV
AVVMLVISFVLLLLINLLQAWTRRHQSGVRAAALPDGNPEVN

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=260, Percent_Identity=51.1538461538461, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI87082099, Length=259, Percent_Identity=32.046332046332, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786980, Length=223, Percent_Identity=33.6322869955157, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787368, Length=242, Percent_Identity=29.3388429752066, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787369, Length=185, Percent_Identity=30.2702702702703, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786254, Length=223, Percent_Identity=27.3542600896861, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30409; Mature: 30409

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPGFGLSLGFTIFYLTLIVLIPLSATFLKTFTMTWDAFWTTVSNPRVVASLELSFGASL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHH
VAAIINTVFGLVVAWVLVRYRFFGKRVIDALVDLPFALPTAVAGIALTALFAGNGWIGRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHH
LEPLGIKVAFTPLGVVVALTFIGLPFVVRTVQPVLEDVERELEEAAASLGASRLQTFSRV
HHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPAILPALLTGFALSFARATGEYGSVVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAV
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AVVMLVISFVLLLLINLLQAWTRRHQSGVRAAALPDGNPEVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
>Mature Secondary Structure
MLPGFGLSLGFTIFYLTLIVLIPLSATFLKTFTMTWDAFWTTVSNPRVVASLELSFGASL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHH
VAAIINTVFGLVVAWVLVRYRFFGKRVIDALVDLPFALPTAVAGIALTALFAGNGWIGRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHH
LEPLGIKVAFTPLGVVVALTFIGLPFVVRTVQPVLEDVERELEEAAASLGASRLQTFSRV
HHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPAILPALLTGFALSFARATGEYGSVVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAV
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AVVMLVISFVLLLLINLLQAWTRRHQSGVRAAALPDGNPEVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]