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The map label for this gene is cysW [H]

Identifier: 94310319

GI number: 94310319

Start: 1486171

End: 1487088

Strand: Direct

Name: cysW [H]

Synonym: Rmet_1377

Alternate gene names: 94310319

Gene position: 1486171-1487088 (Clockwise)

Preceding gene: 94310318

Following gene: 94310320

Centisome position: 37.83

GC content: 64.49

Gene sequence:

>918_bases
ATGGCTGGTGCAATTTCCCGTCTGGGTGGCCCAGGTGCCCCAGGCGCCCCAAGTGCCCACGTACAGGCCGCGCAGTTCCC
GCACGACGCCACTGGCGAATCACGCTGGGTGCGGTACGGGCTGATCACGGTCGCGGTGCTGTTCCTGGGGCTGTTCCTGT
TCATCCCGCTGGCGTCAGTGTTCTATGAGGCGCTGCGCAAGGGTGTCGATACCTATCTGGCTGCATTGACCGAGCCGGAT
GCCGTGTCGGCCATCAAGCTGACGCTGACGGTCGCCGCGATCGCGGTGCCGCTCAATGTGGTGTTCGGCGTGGCCGCCGC
GTGGGCCATCGCCAAGTTCGATTTCCGCGGCAAGAACCTGCTGATCACGCTGATCGACCTGCCGTTCTCGGTGTCGCCTG
TGATCTCCGGCCTGATCTACGTGCTGATGTTCGGCGCGCAGGGTTGGTTCGGACCATGGCTGGAGGCGCACGACATCAAG
ATCATGTTCGCCGTGCCCGGGATCGTGCTGGCGACGATCTTCGTGACGTTCCCGTTCGTGGCGCGCGAGCTGATTCCGCT
GATGCAGGCGCAGGGCAGCGAGGAGGAAGAGGCTGCCATCGTGCTGGGCGCTTCCGGCTGGCAGACGTTCTGGCACGTGA
CGCTGCCGAACATCCGCTGGGGCCTGCTATACGGCGTGATTCTCTGCAACGCGCGGGCGATGGGCGAGTTCGGCGCCGTG
TCGGTGGTATCGGGTCATATCCGCGGCCTGACCAACACCATGCCGCTGCACGTCGAGATTCTCTACAACGAATACAACTT
CGCGGCCGCGTTCGCGGTGGCGTCCCTGCTGACGCTGCTGGCGCTGGTCACGCTCGGTATCAAGACGCTGGTCGAAATCC
GCGCCAGCCGGGAACAACTGGAAGGGCAGCCATCATGA

Upstream 100 bases:

>100_bases
CTGCTGTTGCTGATCAACCTGTTGCAAGCCTGGACGCGCCGTCACCAGAGCGGTGTCCGTGCCGCCGCGCTGCCCGATGG
CAATCCGGAGGTGAACTGAG

Downstream 100 bases:

>100_bases
GCATTCAGGTCAGGAACGTACACAAGCGCTTTGGCGATTTCGTAGCGCTCAACGACGTCTCGCTGGACTTCGCCGAAGGC
GAACTCACCGCGCTGCTGGG

Product: sulfate/thiosulfate ABC transporter membrane protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MAGAISRLGGPGAPGAPSAHVQAAQFPHDATGESRWVRYGLITVAVLFLGLFLFIPLASVFYEALRKGVDTYLAALTEPD
AVSAIKLTLTVAAIAVPLNVVFGVAAAWAIAKFDFRGKNLLITLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLEAHDIK
IMFAVPGIVLATIFVTFPFVARELIPLMQAQGSEEEEAAIVLGASGWQTFWHVTLPNIRWGLLYGVILCNARAMGEFGAV
SVVSGHIRGLTNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVEIRASREQLEGQPS

Sequences:

>Translated_305_residues
MAGAISRLGGPGAPGAPSAHVQAAQFPHDATGESRWVRYGLITVAVLFLGLFLFIPLASVFYEALRKGVDTYLAALTEPD
AVSAIKLTLTVAAIAVPLNVVFGVAAAWAIAKFDFRGKNLLITLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLEAHDIK
IMFAVPGIVLATIFVTFPFVARELIPLMQAQGSEEEEAAIVLGASGWQTFWHVTLPNIRWGLLYGVILCNARAMGEFGAV
SVVSGHIRGLTNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVEIRASREQLEGQPS
>Mature_304_residues
AGAISRLGGPGAPGAPSAHVQAAQFPHDATGESRWVRYGLITVAVLFLGLFLFIPLASVFYEALRKGVDTYLAALTEPDA
VSAIKLTLTVAAIAVPLNVVFGVAAAWAIAKFDFRGKNLLITLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLEAHDIKI
MFAVPGIVLATIFVTFPFVARELIPLMQAQGSEEEEAAIVLGASGWQTFWHVTLPNIRWGLLYGVILCNARAMGEFGAVS
VVSGHIRGLTNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVEIRASREQLEGQPS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=269, Percent_Identity=52.0446096654275, Blast_Score=295, Evalue=2e-81,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=38.8349514563107, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786980, Length=226, Percent_Identity=31.858407079646, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787369, Length=235, Percent_Identity=30.2127659574468, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32693; Mature: 32562

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAISRLGGPGAPGAPSAHVQAAQFPHDATGESRWVRYGLITVAVLFLGLFLFIPLASV
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FYEALRKGVDTYLAALTEPDAVSAIKLTLTVAAIAVPLNVVFGVAAAWAIAKFDFRGKNL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LITLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLEAHDIKIMFAVPGIVLATIFVTFPFV
EEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
ARELIPLMQAQGSEEEEAAIVLGASGWQTFWHVTLPNIRWGLLYGVILCNARAMGEFGAV
HHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCH
SVVSGHIRGLTNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVEIRASREQL
HHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EGQPS
CCCCC
>Mature Secondary Structure 
AGAISRLGGPGAPGAPSAHVQAAQFPHDATGESRWVRYGLITVAVLFLGLFLFIPLASV
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FYEALRKGVDTYLAALTEPDAVSAIKLTLTVAAIAVPLNVVFGVAAAWAIAKFDFRGKNL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LITLIDLPFSVSPVISGLIYVLMFGAQGWFGPWLEAHDIKIMFAVPGIVLATIFVTFPFV
EEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
ARELIPLMQAQGSEEEEAAIVLGASGWQTFWHVTLPNIRWGLLYGVILCNARAMGEFGAV
HHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCH
SVVSGHIRGLTNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVEIRASREQL
HHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EGQPS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]