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The map label for this gene is tomA4 [H]

Identifier: 94310269

GI number: 94310269

Start: 1440133

End: 1440489

Strand: Reverse

Name: tomA4 [H]

Synonym: Rmet_1327

Alternate gene names: 94310269

Gene position: 1440489-1440133 (Counterclockwise)

Preceding gene: 94310270

Following gene: 94310268

Centisome position: 36.67

GC content: 58.54

Gene sequence:

>357_bases
ATGAGCGTTGTTGCCCTCAAACCCTACAAGTTCCCGGCACGAGACGCGCGCGAAAACTTTCCGGCGCCGTTGCTGTTTAT
CGGCTGGGAAGACCATCTGTTGTTTGCGGCACCTGTTGCCTTGCCCCTGCCGTCGGACACGTTGTTCGGTGCGCTGTGCA
CCCAGGTGTTGCCCGGCACTTATGGCTATCACCCCGATTTCTCAAAAATCGACTGGAGCCAGGTGCAGTGGTTTAAGTCC
GGCCAGCCGTGGCATCCCGACCCAGCGAAGTCGCTGGCTGAAAACGGTCTGACGCACAAAGACGTGATCCGCTTTCGCAC
GCCTGGCTTGAACGGTCTGAGCGGTTCCTGCAATTGA

Upstream 100 bases:

>100_bases
ACAACCTGAATGTAGGCCGGGACAACTTCGATTTCGAGGGGTCGGAAGACCAAAAGAACTTTGCTGCCTGGCGTGGAGAG
GTCTTGCAAGGAGAAGCCAA

Downstream 100 bases:

>100_bases
GATTGCGTCATGGCAGCGCAATCACACTGCAGCCGAAGTTACAGGCCGCGGTGTGATTGGCCTCCGGCGATTTGAACAAT
GACCTACCAACTCAACATTC

Product: toluene ortho-monooxygenase P4 protein

Products: NA

Alternate protein names: Phenol 2-monooxygenase P4 component [H]

Number of amino acids: Translated: 118; Mature: 117

Protein sequence:

>118_residues
MSVVALKPYKFPARDARENFPAPLLFIGWEDHLLFAAPVALPLPSDTLFGALCTQVLPGTYGYHPDFSKIDWSQVQWFKS
GQPWHPDPAKSLAENGLTHKDVIRFRTPGLNGLSGSCN

Sequences:

>Translated_118_residues
MSVVALKPYKFPARDARENFPAPLLFIGWEDHLLFAAPVALPLPSDTLFGALCTQVLPGTYGYHPDFSKIDWSQVQWFKS
GQPWHPDPAKSLAENGLTHKDVIRFRTPGLNGLSGSCN
>Mature_117_residues
SVVALKPYKFPARDARENFPAPLLFIGWEDHLLFAAPVALPLPSDTLFGALCTQVLPGTYGYHPDFSKIDWSQVQWFKSG
QPWHPDPAKSLAENGLTHKDVIRFRTPGLNGLSGSCN

Specific function: Catabolizes phenol, and some of its methylated derivatives. P4 is required for growth on phenol, and for in vitro phenol hydroxylase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006756 [H]

Pfam domain/function: PF04663 Phenol_monoox [H]

EC number: =1.14.13.7 [H]

Molecular weight: Translated: 13079; Mature: 12948

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVALKPYKFPARDARENFPAPLLFIGWEDHLLFAAPVALPLPSDTLFGALCTQVLPGT
CCEEECCCCCCCCCCHHHCCCCCEEEECCCCCEEEECCEECCCCCHHHHHHHHHHHCCCC
YGYHPDFSKIDWSQVQWFKSGQPWHPDPAKSLAENGLTHKDVIRFRTPGLNGLSGSCN
CCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SVVALKPYKFPARDARENFPAPLLFIGWEDHLLFAAPVALPLPSDTLFGALCTQVLPGT
CEEECCCCCCCCCCHHHCCCCCEEEECCCCCEEEECCEECCCCCHHHHHHHHHHHCCCC
YGYHPDFSKIDWSQVQWFKSGQPWHPDPAKSLAENGLTHKDVIRFRTPGLNGLSGSCN
CCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254258 [H]