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The map label for this gene is tomA3 [H]

Identifier: 94310270

GI number: 94310270

Start: 1440486

End: 1442045

Strand: Reverse

Name: tomA3 [H]

Synonym: Rmet_1328

Alternate gene names: 94310270

Gene position: 1442045-1440486 (Counterclockwise)

Preceding gene: 94310271

Following gene: 94310269

Centisome position: 36.71

GC content: 55.13

Gene sequence:

>1560_bases
ATGGACACTTCTGTGCAGAAGAAGAAACTCGGTTTAAAGGATCGCTACGCAGCGATGACCCGCGGTCTTGGCTGGCAGAC
CAGCTACCAGCCGATGGAAAAAGTGTTTCCGTACGACAAGTACGAAAGCATAAAGATCCACGATTGGGATAAATGGGAAG
ACCCCTTCCGCCTGACCATGGACGCCTACTGGAAATATCAGGGCGAGAAGGAAAAAAAGCTTTACGCCGTCATCGACGCT
TTCGCGCAGAACAACGGGCAGTTGAGCATTTCCGACGCGCGCTATGTCAACGCACTCAAGGTGTTTATCCAGGGTGTGAC
ACCGCTGGAGTATATGGCACACCGAGGTTTTGCCCACGTTGGTCGGCATTTTACGGGTGAAGGGGCGCGTGTTGCTTGCC
AGATGCAGTCCATCGACGAGCTGCGTCACTTCCAGACCGAAATGCATGCTCTCTCGCACTACAACAAGTATTTTAACGGC
CTGCACAACTCCATCCATTGGTACGACCGGGTCTGGTATTTGTCGGTGCCCAAGTCATTTTTTGAAGACGCGGCCACCGG
TGGACCGTTCGAGTTTCTTACCGCGGTGAGCTTTTCGTTCGAATATGTGTTGACCAACCTGCTGTTTGTCCCCTTCATGT
CGGGTGCTGCTTACAACGGGGACATGTCGACGGTCACCTTCGGTTTTTCGGCGCAAAGTGACGAATCGCGCCACATGACA
CTCGGCATCGAATGCATCAAGTTCATGCTGGAACAGGATCCGGACAACGTGCCCATCGTGCAGCGCTGGATCGACAAGTG
GTTCTGGCGCGGCTATCGGCTGTTGAGCATCGTGGCCATGATGCAGGACTACATGCTGCCCAACCGGGTGATGAGCTGGC
GCGAGAGCTGGGAGATGTACGTCGAGCAGAACGGCGGCGCGCTGTTCAAGGATCTGGCGCGTTACGGCATCCGCAAGCCC
AAGGGCTGGGACCAGGCTTGCGAAGGCAAGGACCACATCAGCCATCAGACCTTCGCCGTGTTCTATAACTATAACGCCGC
GGCCCCCATCCACACCTGGGTTCCCACAGAAGAAGAAATGGGGTGGCTGTCGGAGAAGTACCCCGAGACGTTCGACAAGT
ATTACCGTCCGCGTTGGGACTACTGGCGCGAGCAGGCCGCCAAGGGCAACCGTTTCTACAACAAGACGCTGCCGATGCTC
TGCACTACCTGCCAGATTCCGATGATATTCACCGAGCCTGGCGACGCAACCAAGATCTGCTATCGCGAGTCGGACTACCT
CGGCGACAAGTATCACTTCTGCAGCGACCACTGCAAGGAGATTTTTGACAACGAACCCGAGAAGTTCGTGCAGTCATGGC
TTCCGCCGCAGCAAGTGTATCAAGGAAACTGTTTCAAGCCGGATGCCGATCCGACCAAGGAAGGTTTCGATCCCTTGATG
GCCTTGCTCGACTACTACAACCTGAATGTAGGCCGGGACAACTTCGATTTCGAGGGGTCGGAAGACCAAAAGAACTTTGC
TGCCTGGCGTGGAGAGGTCTTGCAAGGAGAAGCCAAATGA

Upstream 100 bases:

>100_bases
ATCCACATCAACCTAATCACCTTGTCCGGATATATCGACGAGGATGACGAACAGTTCACGCTGAGCTGGAAACACTGAAC
ACGGCAAAGAGGAAATTGAA

Downstream 100 bases:

>100_bases
GCGTTGTTGCCCTCAAACCCTACAAGTTCCCGGCACGAGACGCGCGCGAAAACTTTCCGGCGCCGTTGCTGTTTATCGGC
TGGGAAGACCATCTGTTGTT

Product: toluene hydroxylase P3 protein

Products: NA

Alternate protein names: Phenol 2-monooxygenase P3 component [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MDTSVQKKKLGLKDRYAAMTRGLGWQTSYQPMEKVFPYDKYESIKIHDWDKWEDPFRLTMDAYWKYQGEKEKKLYAVIDA
FAQNNGQLSISDARYVNALKVFIQGVTPLEYMAHRGFAHVGRHFTGEGARVACQMQSIDELRHFQTEMHALSHYNKYFNG
LHNSIHWYDRVWYLSVPKSFFEDAATGGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAYNGDMSTVTFGFSAQSDESRHMT
LGIECIKFMLEQDPDNVPIVQRWIDKWFWRGYRLLSIVAMMQDYMLPNRVMSWRESWEMYVEQNGGALFKDLARYGIRKP
KGWDQACEGKDHISHQTFAVFYNYNAAAPIHTWVPTEEEMGWLSEKYPETFDKYYRPRWDYWREQAAKGNRFYNKTLPML
CTTCQIPMIFTEPGDATKICYRESDYLGDKYHFCSDHCKEIFDNEPEKFVQSWLPPQQVYQGNCFKPDADPTKEGFDPLM
ALLDYYNLNVGRDNFDFEGSEDQKNFAAWRGEVLQGEAK

Sequences:

>Translated_519_residues
MDTSVQKKKLGLKDRYAAMTRGLGWQTSYQPMEKVFPYDKYESIKIHDWDKWEDPFRLTMDAYWKYQGEKEKKLYAVIDA
FAQNNGQLSISDARYVNALKVFIQGVTPLEYMAHRGFAHVGRHFTGEGARVACQMQSIDELRHFQTEMHALSHYNKYFNG
LHNSIHWYDRVWYLSVPKSFFEDAATGGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAYNGDMSTVTFGFSAQSDESRHMT
LGIECIKFMLEQDPDNVPIVQRWIDKWFWRGYRLLSIVAMMQDYMLPNRVMSWRESWEMYVEQNGGALFKDLARYGIRKP
KGWDQACEGKDHISHQTFAVFYNYNAAAPIHTWVPTEEEMGWLSEKYPETFDKYYRPRWDYWREQAAKGNRFYNKTLPML
CTTCQIPMIFTEPGDATKICYRESDYLGDKYHFCSDHCKEIFDNEPEKFVQSWLPPQQVYQGNCFKPDADPTKEGFDPLM
ALLDYYNLNVGRDNFDFEGSEDQKNFAAWRGEVLQGEAK
>Mature_519_residues
MDTSVQKKKLGLKDRYAAMTRGLGWQTSYQPMEKVFPYDKYESIKIHDWDKWEDPFRLTMDAYWKYQGEKEKKLYAVIDA
FAQNNGQLSISDARYVNALKVFIQGVTPLEYMAHRGFAHVGRHFTGEGARVACQMQSIDELRHFQTEMHALSHYNKYFNG
LHNSIHWYDRVWYLSVPKSFFEDAATGGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAYNGDMSTVTFGFSAQSDESRHMT
LGIECIKFMLEQDPDNVPIVQRWIDKWFWRGYRLLSIVAMMQDYMLPNRVMSWRESWEMYVEQNGGALFKDLARYGIRKP
KGWDQACEGKDHISHQTFAVFYNYNAAAPIHTWVPTEEEMGWLSEKYPETFDKYYRPRWDYWREQAAKGNRFYNKTLPML
CTTCQIPMIFTEPGDATKICYRESDYLGDKYHFCSDHCKEIFDNEPEKFVQSWLPPQQVYQGNCFKPDADPTKEGFDPLM
ALLDYYNLNVGRDNFDFEGSEDQKNFAAWRGEVLQGEAK

Specific function: Catabolizes phenol, and some of its methylated derivatives. P3 is required for growth on phenol, and for in vitro phenol hydroxylase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR003430
- InterPro:   IPR012348
- InterPro:   IPR007029 [H]

Pfam domain/function: PF02332 Phenol_Hydrox; PF04945 YHS [H]

EC number: =1.14.13.7 [H]

Molecular weight: Translated: 60983; Mature: 60983

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSVQKKKLGLKDRYAAMTRGLGWQTSYQPMEKVFPYDKYESIKIHDWDKWEDPFRLTM
CCCCCCHHHCCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCEEEE
DAYWKYQGEKEKKLYAVIDAFAQNNGQLSISDARYVNALKVFIQGVTPLEYMAHRGFAHV
EHHHEECCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCHHHHHHHCCHHHH
GRHFTGEGARVACQMQSIDELRHFQTEMHALSHYNKYFNGLHNSIHWYDRVWYLSVPKSF
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCHHH
FEDAATGGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAYNGDMSTVTFGFSAQSDESRHMT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEE
LGIECIKFMLEQDPDNVPIVQRWIDKWFWRGYRLLSIVAMMQDYMLPNRVMSWRESWEMY
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VEQNGGALFKDLARYGIRKPKGWDQACEGKDHISHQTFAVFYNYNAAAPIHTWVPTEEEM
HCCCCCHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCEEEEEEECCCCCCCEECCCCHHHH
GWLSEKYPETFDKYYRPRWDYWREQAAKGNRFYNKTLPMLCTTCQIPMIFTEPGDATKIC
HHHHHHCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEE
YRESDYLGDKYHFCSDHCKEIFDNEPEKFVQSWLPPQQVYQGNCFKPDADPTKEGFDPLM
EECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHH
ALLDYYNLNVGRDNFDFEGSEDQKNFAAWRGEVLQGEAK
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCCC
>Mature Secondary Structure
MDTSVQKKKLGLKDRYAAMTRGLGWQTSYQPMEKVFPYDKYESIKIHDWDKWEDPFRLTM
CCCCCCHHHCCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCEEEE
DAYWKYQGEKEKKLYAVIDAFAQNNGQLSISDARYVNALKVFIQGVTPLEYMAHRGFAHV
EHHHEECCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCHHHHHHHCCHHHH
GRHFTGEGARVACQMQSIDELRHFQTEMHALSHYNKYFNGLHNSIHWYDRVWYLSVPKSF
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCHHH
FEDAATGGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAYNGDMSTVTFGFSAQSDESRHMT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEE
LGIECIKFMLEQDPDNVPIVQRWIDKWFWRGYRLLSIVAMMQDYMLPNRVMSWRESWEMY
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VEQNGGALFKDLARYGIRKPKGWDQACEGKDHISHQTFAVFYNYNAAAPIHTWVPTEEEM
HCCCCCHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCEEEEEEECCCCCCCEECCCCHHHH
GWLSEKYPETFDKYYRPRWDYWREQAAKGNRFYNKTLPMLCTTCQIPMIFTEPGDATKIC
HHHHHHCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEE
YRESDYLGDKYHFCSDHCKEIFDNEPEKFVQSWLPPQQVYQGNCFKPDADPTKEGFDPLM
EECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHH
ALLDYYNLNVGRDNFDFEGSEDQKNFAAWRGEVLQGEAK
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2254258 [H]