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The map label for this gene is hoxG [H]

Identifier: 94310241

GI number: 94310241

Start: 1412448

End: 1414307

Strand: Reverse

Name: hoxG [H]

Synonym: Rmet_1297

Alternate gene names: 94310241

Gene position: 1414307-1412448 (Counterclockwise)

Preceding gene: 94310242

Following gene: 94310240

Centisome position: 36.0

GC content: 66.13

Gene sequence:

>1860_bases
ATGGGTGCTTACGAAACTCAAGGCTTCCGCATGGACAACACGGGCCGGCGCATCGTCGTCGACCCGGTGACCCGCATCGA
GGGCCACTTGCGCTGCGAGGTCAACCTCGACAGCAACAACGTGATCCGCAACGCCGTCTCCACCGGCACCATGTGGCGCG
GGCTGGAGGTCATCCTCAAGGGGCGCGACCCGCGCGACGCCTGGGCCTTCGTGGAGCGCATCTGCGGCGTGTGCACCGGC
TGCCACGCGCTCGCCTCGGTGCGCGCGGTGGAGGACGCGCTGGGCATCCGCATCCCGCTCAACGCGCACCTGATCCGCGA
GATGATGGCCAAGACGCTGCAGGTGCACGACCACGCGGTGCACTTCTACCACCTGCACGCGCTCGACTGGGTGGACGTGG
TGTCGGCGCTGAACGCCGACCCGAAGAAGACCAGCGATCTGCAGCACATCGTCTCGCCCTCGCACCCGATGTCCTCGCCG
GGTTACTTCCGCGACGTGCAGAACCGCCTGAAGAAGTTCGTCGAGAGCGGCCAGCTCGGCCCCTTCGCCAATGGCTACTG
GGGCTCCAAGGCTTACGTGCTGCCGGCCGAGGCCAACCTGATGGCGGTGACCCATTACCTGGAGGCGCTGGACCTGCAGA
AGGAATGGGTCAAGGTGCACACCATCTTCGGCGGCAAGAACCCGCACCCCAACTACATGGTGGGCGGCGTGCCCTGCGCG
ATCAACATCGACGGCGACGGCGCGGCCGGCGCGCCCATCAACATGGAGCGCCTGAACTTCGTCGAGGCCCGCATCAAGGA
GATGATCGACTTCAACAACAACGTCTACATCCCGGACGTGCTGGCCATCGGCACCCTCTACAAACAGGCGGGCTGGCTCT
ACGGCGGCGGGCTTTCCGCGCTCAACGTGGCCGACTACGGCACCTACGAGAAGGTGCCCTACGACCACAGCACGCACCAG
TTGCCGGGCGGCGTGATCCTGGACGGCGACTGGAGCAAGATCCACGAGATCGACCCGCGCGACCCGGAACAGGTGCAGGA
GTTCGTGACCCACTCCTGGTACAAGTACGCCGATGAAAGCCAGGGCCTGCACCCCTGGGACGGCGTGACCGAGGCGAACT
ACCGGCCCGAAGGCCCGAACTTCAAGGGCACGCGCACGAAGATCGAGCAGCTCGACGAATCAGCCAAGTACTCGTGGATC
AAGTCGCCGCGCTGGCGCGGCCACGCGGTCGAGGTCGGTCCGCTCTCGCGCTACATCCTGGGCTACGCGCACGCGCTGCA
GGGCAACCAGTGGTGCCAGCGCGTCAAGCAGCAGGTGGACGAGGCGGCCACTGCCATCAACAGCGCCATCCCCAAGGCGC
TGGGCCTGCCCGAGACGAACTACAGCGCCAAGCAACTGCTGCCCACCACCATCGGCCGCACGCTGGCACGGGCGCTGGAA
AGCCAGTACGCCGCCGAGATGATGATGGACGACTTCCGCCAGCTGGTGCAGAACATCAAGGCCGGCGACACGTCCACCGC
CAACGTCGAGAAGTGGGACCCCAAGACCTGGCCCAAGGAGGCCAAGGGCGTGGGCACGGTGGGCGCGCCGCGCGGCATGC
TGGGCCACTGGATCCGGATCAAGGATGGCAGGATCGAGAATTACCAGTGCGTGGTGCCCACCACCTGGAACGGCTCTCCA
CGCGACCACAAGGGCCAGATCGGCGCCTTCGAGGCCTCGCTGATGAACACGCCCATGGTCAACCCCGAGCAGCCGCTGGA
GATCCTGCGCACGCTGCACAGCTTCGACCCTTGCCTGGCTTGCTCCACCCACGTGATGAGCGAAGACGGCCAGGAGATGA
GCCGGGTGAAGGTGAGATGA

Upstream 100 bases:

>100_bases
ACGCGGCCATCTCGGTGGCCAAGCGCGTGCGCGACAACAACGCCCGCAAGCCCGACGCATCCACACCCGCCAACCACTGA
CACGACCGAGGAACAAGAAC

Downstream 100 bases:

>100_bases
GCCCCCGCCTGCGGCGCTTCGCGTCTTCCCCCCGAAGGGGGGACGCACCTGGTGGTCCGGCGGAGCCGGTTCCAAGGGTG
CCCTGGACAAGACCGCCGCA

Product: HoxG hydrogenase 1, large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MGAYETQGFRMDNTGRRIVVDPVTRIEGHLRCEVNLDSNNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTG
CHALASVRAVEDALGIRIPLNAHLIREMMAKTLQVHDHAVHFYHLHALDWVDVVSALNADPKKTSDLQHIVSPSHPMSSP
GYFRDVQNRLKKFVESGQLGPFANGYWGSKAYVLPAEANLMAVTHYLEALDLQKEWVKVHTIFGGKNPHPNYMVGGVPCA
INIDGDGAAGAPINMERLNFVEARIKEMIDFNNNVYIPDVLAIGTLYKQAGWLYGGGLSALNVADYGTYEKVPYDHSTHQ
LPGGVILDGDWSKIHEIDPRDPEQVQEFVTHSWYKYADESQGLHPWDGVTEANYRPEGPNFKGTRTKIEQLDESAKYSWI
KSPRWRGHAVEVGPLSRYILGYAHALQGNQWCQRVKQQVDEAATAINSAIPKALGLPETNYSAKQLLPTTIGRTLARALE
SQYAAEMMMDDFRQLVQNIKAGDTSTANVEKWDPKTWPKEAKGVGTVGAPRGMLGHWIRIKDGRIENYQCVVPTTWNGSP
RDHKGQIGAFEASLMNTPMVNPEQPLEILRTLHSFDPCLACSTHVMSEDGQEMSRVKVR

Sequences:

>Translated_619_residues
MGAYETQGFRMDNTGRRIVVDPVTRIEGHLRCEVNLDSNNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTG
CHALASVRAVEDALGIRIPLNAHLIREMMAKTLQVHDHAVHFYHLHALDWVDVVSALNADPKKTSDLQHIVSPSHPMSSP
GYFRDVQNRLKKFVESGQLGPFANGYWGSKAYVLPAEANLMAVTHYLEALDLQKEWVKVHTIFGGKNPHPNYMVGGVPCA
INIDGDGAAGAPINMERLNFVEARIKEMIDFNNNVYIPDVLAIGTLYKQAGWLYGGGLSALNVADYGTYEKVPYDHSTHQ
LPGGVILDGDWSKIHEIDPRDPEQVQEFVTHSWYKYADESQGLHPWDGVTEANYRPEGPNFKGTRTKIEQLDESAKYSWI
KSPRWRGHAVEVGPLSRYILGYAHALQGNQWCQRVKQQVDEAATAINSAIPKALGLPETNYSAKQLLPTTIGRTLARALE
SQYAAEMMMDDFRQLVQNIKAGDTSTANVEKWDPKTWPKEAKGVGTVGAPRGMLGHWIRIKDGRIENYQCVVPTTWNGSP
RDHKGQIGAFEASLMNTPMVNPEQPLEILRTLHSFDPCLACSTHVMSEDGQEMSRVKVR
>Mature_618_residues
GAYETQGFRMDNTGRRIVVDPVTRIEGHLRCEVNLDSNNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTGC
HALASVRAVEDALGIRIPLNAHLIREMMAKTLQVHDHAVHFYHLHALDWVDVVSALNADPKKTSDLQHIVSPSHPMSSPG
YFRDVQNRLKKFVESGQLGPFANGYWGSKAYVLPAEANLMAVTHYLEALDLQKEWVKVHTIFGGKNPHPNYMVGGVPCAI
NIDGDGAAGAPINMERLNFVEARIKEMIDFNNNVYIPDVLAIGTLYKQAGWLYGGGLSALNVADYGTYEKVPYDHSTHQL
PGGVILDGDWSKIHEIDPRDPEQVQEFVTHSWYKYADESQGLHPWDGVTEANYRPEGPNFKGTRTKIEQLDESAKYSWIK
SPRWRGHAVEVGPLSRYILGYAHALQGNQWCQRVKQQVDEAATAINSAIPKALGLPETNYSAKQLLPTTIGRTLARALES
QYAAEMMMDDFRQLVQNIKAGDTSTANVEKWDPKTWPKEAKGVGTVGAPRGMLGHWIRIKDGRIENYQCVVPTTWNGSPR
DHKGQIGAFEASLMNTPMVNPEQPLEILRTLHSFDPCLACSTHVMSEDGQEMSRVKVR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=618, Percent_Identity=57.9288025889968, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=610, Percent_Identity=42.7868852459016, Blast_Score=461, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 69081; Mature: 68950

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAYETQGFRMDNTGRRIVVDPVTRIEGHLRCEVNLDSNNVIRNAVSTGTMWRGLEVILK
CCCCCCCCEEECCCCCEEEECCHHHCCCEEEEEEECCCCHHHHHHHHHCHHHHCHHHEEE
GRDPRDAWAFVERICGVCTGCHALASVRAVEDALGIRIPLNAHLIREMMAKTLQVHDHAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHEE
HFYHLHALDWVDVVSALNADPKKTSDLQHIVSPSHPMSSPGYFRDVQNRLKKFVESGQLG
EEEEEHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
PFANGYWGSKAYVLPAEANLMAVTHYLEALDLQKEWVKVHTIFGGKNPHPNYMVGGVPCA
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCEEEE
INIDGDGAAGAPINMERLNFVEARIKEMIDFNNNVYIPDVLAIGTLYKQAGWLYGGGLSA
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCCCEECCCCCE
LNVADYGTYEKVPYDHSTHQLPGGVILDGDWSKIHEIDPRDPEQVQEFVTHSWYKYADES
EEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
QGLHPWDGVTEANYRPEGPNFKGTRTKIEQLDESAKYSWIKSPRWRGHAVEVGPLSRYIL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHH
GYAHALQGNQWCQRVKQQVDEAATAINSAIPKALGLPETNYSAKQLLPTTIGRTLARALE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SQYAAEMMMDDFRQLVQNIKAGDTSTANVEKWDPKTWPKEAKGVGTVGAPRGMLGHWIRI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHCCEEEE
KDGRIENYQCVVPTTWNGSPRDHKGQIGAFEASLMNTPMVNPEQPLEILRTLHSFDPCLA
ECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
CSTHVMSEDGQEMSRVKVR
HHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure 
GAYETQGFRMDNTGRRIVVDPVTRIEGHLRCEVNLDSNNVIRNAVSTGTMWRGLEVILK
CCCCCCCEEECCCCCEEEECCHHHCCCEEEEEEECCCCHHHHHHHHHCHHHHCHHHEEE
GRDPRDAWAFVERICGVCTGCHALASVRAVEDALGIRIPLNAHLIREMMAKTLQVHDHAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHEE
HFYHLHALDWVDVVSALNADPKKTSDLQHIVSPSHPMSSPGYFRDVQNRLKKFVESGQLG
EEEEEHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
PFANGYWGSKAYVLPAEANLMAVTHYLEALDLQKEWVKVHTIFGGKNPHPNYMVGGVPCA
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCEEEE
INIDGDGAAGAPINMERLNFVEARIKEMIDFNNNVYIPDVLAIGTLYKQAGWLYGGGLSA
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCCCEECCCCCE
LNVADYGTYEKVPYDHSTHQLPGGVILDGDWSKIHEIDPRDPEQVQEFVTHSWYKYADES
EEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
QGLHPWDGVTEANYRPEGPNFKGTRTKIEQLDESAKYSWIKSPRWRGHAVEVGPLSRYIL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHH
GYAHALQGNQWCQRVKQQVDEAATAINSAIPKALGLPETNYSAKQLLPTTIGRTLARALE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SQYAAEMMMDDFRQLVQNIKAGDTSTANVEKWDPKTWPKEAKGVGTVGAPRGMLGHWIRI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHCCEEEE
KDGRIENYQCVVPTTWNGSPRDHKGQIGAFEASLMNTPMVNPEQPLEILRTLHSFDPCLA
ECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
CSTHVMSEDGQEMSRVKVR
HHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2325631 [H]