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The map label for this gene is boxB [H]

Identifier: 94310166

GI number: 94310166

Start: 1339141

End: 1340565

Strand: Reverse

Name: boxB [H]

Synonym: Rmet_1221

Alternate gene names: 94310166

Gene position: 1340565-1339141 (Counterclockwise)

Preceding gene: 94310167

Following gene: 94310165

Centisome position: 34.13

GC content: 62.88

Gene sequence:

>1425_bases
ATGAGCATCGACTACAGCCAGAAGATTCCGAACAACGTGAACCTCTCCGAGGACCGCACGCTGCAGCGCGCGCTCGAACA
CTGGCAGCCGGCGTTCCTGGACTGGTGGCGCGACATGGGGCCGGACGGCTCGCACAACTTCGATGTCTACCTGCGCACCG
CGGTCTCCGTCGATCCGTCAGGCTGGGCGCACTTCGAGCACGTGAAGATGCCCGACTATCGCTGGGGTATCTTCCTGCAA
CCGGCAGATCCCAATCGCCGGATTCACTTCGGCGAGCACAAGGGTGAGGCGGCCTGGCAGGAAGTACCGGGCGAACACCG
CGCCAATCTGCGCCGCATCATCGTCACGCAGGGTGACACGGAACCGGCATCGGTGGAGCAGCAACGGCATCTGGGCCTGA
CCGCGCCGTCGCTCTATGACCTGCGCAACCTGTTCCAGGTCAACGTGGAAGAAGGCCGACACCTGTGGGCCATGGTCTAC
CTGCTGCACCGCCATTTCGGTCGCGACGGCCGTGAGGAAGCGGAAGCGCTGCTCGGCCGCCGCTCGGGTGACGAGGACAA
TCCACGCATCCTGGGCGCGTTCAACGAGCGGACCCCGGACTGGCTGTCGTTCTTCATGTTTACCTACTTCACCGACCGCG
ACGGCAAGTTCCAGCTCTGCGCGCTGGCTGAATCTGGCTTCGACCCGCTTGCGCGCACCACGCGCTTCATGCTGACCGAG
GAAGCACACCATATGTTTGTGGGTGAGTCCGGCGTCTCACGAGTGATCCAGCGCACCTGCGAGGTCATGCGCGAGCGCGA
CATCCAGGACCCGGCGGACGTGCGCGCCGCTGGTGTGATCGACCTGGAAACGATCCAGCGCTACCTGAACTTCCACTACA
GCGTCACGATCGACCTGTTCGGGGCGGACCAGTCTTCCAACGCCGCCACGTTCTACAGCGCCGGCCTCAAGGGTCGGTTC
GACGAAGGCAAGCGCGACGACGATCACGTGCTCAAGGGCGACGTGTACCGCATCCTCGACGTCCGGGATGGCAAGCTGAC
CGAGCGTGAAGTGCCGATGCTGAATGCGTTGAACGAGGTGCTGCGCGACGACTACATTAAAGACTCCATGGGCGGCGTGG
CGCGCTGGAACAAGGTCATCGAGAAGGCAGGCATCCCGTTCCGCCTGACCGTGCCGCACAAGGCGTTCAACCGCAAGATC
GGCACCTTCGCAAACCTGCACGTGTCGCCCACCGGCGAAATACTGACGACCGCCGAATGGGAAGCCAACAAGGACAAATG
GCTGGCGACCGAACAAGATCGCAAGTACGTGGCTTCGCTGATGGGGAGGGTGGTAGAACCCGGCAAGTACGCCAACTGGA
TCGCGCCGCCGGCTGGTGGCATCAACCGCCAGCCGATTGATTTCGAGTACGTTCGTTTCAACTGA

Upstream 100 bases:

>100_bases
GCGCGCTCAAGGTATTCGGCACCGGTAACAAGGCACGCTTCGACTGGGATCGGGTCTGACGACTGTCCGATCTGACGGCA
AATATAAAAGGAGACCCGCA

Downstream 100 bases:

>100_bases
CGACCTATCCATCGAGGCTGACGATCATGGGCGCCGCCGACATCATCAAGCAACATCTGATCGACCCGGAAATCTGCATT
CGCTGCAACACCTGCGAAGA

Product: benzoyl-CoA oxygenase component B

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit B; Benzoyl-CoA dioxygenase oxygenase component [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSIDYSQKIPNNVNLSEDRTLQRALEHWQPAFLDWWRDMGPDGSHNFDVYLRTAVSVDPSGWAHFEHVKMPDYRWGIFLQ
PADPNRRIHFGEHKGEAAWQEVPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSLYDLRNLFQVNVEEGRHLWAMVY
LLHRHFGRDGREEAEALLGRRSGDEDNPRILGAFNERTPDWLSFFMFTYFTDRDGKFQLCALAESGFDPLARTTRFMLTE
EAHHMFVGESGVSRVIQRTCEVMRERDIQDPADVRAAGVIDLETIQRYLNFHYSVTIDLFGADQSSNAATFYSAGLKGRF
DEGKRDDDHVLKGDVYRILDVRDGKLTEREVPMLNALNEVLRDDYIKDSMGGVARWNKVIEKAGIPFRLTVPHKAFNRKI
GTFANLHVSPTGEILTTAEWEANKDKWLATEQDRKYVASLMGRVVEPGKYANWIAPPAGGINRQPIDFEYVRFN

Sequences:

>Translated_474_residues
MSIDYSQKIPNNVNLSEDRTLQRALEHWQPAFLDWWRDMGPDGSHNFDVYLRTAVSVDPSGWAHFEHVKMPDYRWGIFLQ
PADPNRRIHFGEHKGEAAWQEVPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSLYDLRNLFQVNVEEGRHLWAMVY
LLHRHFGRDGREEAEALLGRRSGDEDNPRILGAFNERTPDWLSFFMFTYFTDRDGKFQLCALAESGFDPLARTTRFMLTE
EAHHMFVGESGVSRVIQRTCEVMRERDIQDPADVRAAGVIDLETIQRYLNFHYSVTIDLFGADQSSNAATFYSAGLKGRF
DEGKRDDDHVLKGDVYRILDVRDGKLTEREVPMLNALNEVLRDDYIKDSMGGVARWNKVIEKAGIPFRLTVPHKAFNRKI
GTFANLHVSPTGEILTTAEWEANKDKWLATEQDRKYVASLMGRVVEPGKYANWIAPPAGGINRQPIDFEYVRFN
>Mature_473_residues
SIDYSQKIPNNVNLSEDRTLQRALEHWQPAFLDWWRDMGPDGSHNFDVYLRTAVSVDPSGWAHFEHVKMPDYRWGIFLQP
ADPNRRIHFGEHKGEAAWQEVPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSLYDLRNLFQVNVEEGRHLWAMVYL
LHRHFGRDGREEAEALLGRRSGDEDNPRILGAFNERTPDWLSFFMFTYFTDRDGKFQLCALAESGFDPLARTTRFMLTEE
AHHMFVGESGVSRVIQRTCEVMRERDIQDPADVRAAGVIDLETIQRYLNFHYSVTIDLFGADQSSNAATFYSAGLKGRFD
EGKRDDDHVLKGDVYRILDVRDGKLTEREVPMLNALNEVLRDDYIKDSMGGVARWNKVIEKAGIPFRLTVPHKAFNRKIG
TFANLHVSPTGEILTTAEWEANKDKWLATEQDRKYVASLMGRVVEPGKYANWIAPPAGGINRQPIDFEYVRFN

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxB acts as the benzoyl-CoA oxygenase, after being reduced by the reductase component BoxA. BoxAB does not

COG id: COG3396

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the benzoyl-CoA oxygenase component B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017635
- InterPro:   IPR012347
- InterPro:   IPR009078
- InterPro:   IPR007814 [H]

Pfam domain/function: PF05138 PaaA_PaaC [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 54510; Mature: 54379

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDYSQKIPNNVNLSEDRTLQRALEHWQPAFLDWWRDMGPDGSHNFDVYLRTAVSVDPS
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEEEEEEECCC
GWAHFEHVKMPDYRWGIFLQPADPNRRIHFGEHKGEAAWQEVPGEHRANLRRIIVTQGDT
CCCEEEECCCCCCCEEEEEECCCCCCCEECCCCCCCHHHHHCCCHHHCCEEEEEEECCCC
EPASVEQQRHLGLTAPSLYDLRNLFQVNVEEGRHLWAMVYLLHRHFGRDGREEAEALLGR
CCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
RSGDEDNPRILGAFNERTPDWLSFFMFTYFTDRDGKFQLCALAESGFDPLARTTRFMLTE
CCCCCCCCEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCHHHHHHHHHEEC
EAHHMFVGESGVSRVIQRTCEVMRERDIQDPADVRAAGVIDLETIQRYLNFHYSVTIDLF
CCHHEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEHHHHHHHHCCEEEEEEEEE
GADQSSNAATFYSAGLKGRFDEGKRDDDHVLKGDVYRILDVRDGKLTEREVPMLNALNEV
ECCCCCCCEEEEECCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCHHCCHHHHHHHHH
LRDDYIKDSMGGVARWNKVIEKAGIPFRLTVPHKAFNRKIGTFANLHVSPTGEILTTAEW
HHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEEEECCCCCEEEEECC
EANKDKWLATEQDRKYVASLMGRVVEPGKYANWIAPPAGGINRQPIDFEYVRFN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
SIDYSQKIPNNVNLSEDRTLQRALEHWQPAFLDWWRDMGPDGSHNFDVYLRTAVSVDPS
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEEEEEEECCC
GWAHFEHVKMPDYRWGIFLQPADPNRRIHFGEHKGEAAWQEVPGEHRANLRRIIVTQGDT
CCCEEEECCCCCCCEEEEEECCCCCCCEECCCCCCCHHHHHCCCHHHCCEEEEEEECCCC
EPASVEQQRHLGLTAPSLYDLRNLFQVNVEEGRHLWAMVYLLHRHFGRDGREEAEALLGR
CCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
RSGDEDNPRILGAFNERTPDWLSFFMFTYFTDRDGKFQLCALAESGFDPLARTTRFMLTE
CCCCCCCCEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCHHHHHHHHHEEC
EAHHMFVGESGVSRVIQRTCEVMRERDIQDPADVRAAGVIDLETIQRYLNFHYSVTIDLF
CCHHEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEHHHHHHHHCCEEEEEEEEE
GADQSSNAATFYSAGLKGRFDEGKRDDDHVLKGDVYRILDVRDGKLTEREVPMLNALNEV
ECCCCCCCEEEEECCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCHHCCHHHHHHHHH
LRDDYIKDSMGGVARWNKVIEKAGIPFRLTVPHKAFNRKIGTFANLHVSPTGEILTTAEW
HHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEEEECCCCCEEEEECC
EANKDKWLATEQDRKYVASLMGRVVEPGKYANWIAPPAGGINRQPIDFEYVRFN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]