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The map label for this gene is boxA [H]

Identifier: 94310165

GI number: 94310165

Start: 1337867

End: 1339114

Strand: Reverse

Name: boxA [H]

Synonym: Rmet_1220

Alternate gene names: 94310165

Gene position: 1339114-1337867 (Counterclockwise)

Preceding gene: 94310166

Following gene: 94310164

Centisome position: 34.09

GC content: 64.26

Gene sequence:

>1248_bases
ATGGGCGCCGCCGACATCATCAAGCAACATCTGATCGACCCGGAAATCTGCATTCGCTGCAACACCTGCGAAGACACGTG
CCCGATAGATGCCATCACTCACGACGACCGCAACTACGTGGTCAAGGCTGACGTCTGCAATGCGTGCAATGCATGCCTGT
CGCCCTGCCCCACCGGCGCCATCGACAACTGGCGCACGATGCTCAAGGGCCAGGCCTACACCATCGAAGCCCAGTTGACC
TGGGACGAACTGCCGGAAGAGGTGCCGTTGCCGGAAGCGGAGATCGAGGCAGCGGCTGCGGCCGGGCAGGTCATCGAGGA
AGCGTCGCGCGGCAGCAAATCCGTGGCCGTTCAGGAGGTGGAGGCCAGTCGGCATACGTCATCCAGGGCGCCGTGGTCGG
CCGCGCATCCGTACGTCAACCTGCATGGCGTGCGCGAGCCGGTGACCGCCACAGTGGCCGGCAACCACCGGCTGACCGCC
GAGGATGCATCGAGCGACATCCATCACATCGTGCTCGACTTCGGCAATCACTTCTTTCCGGTGCTGGAAGGTCAGGCAAT
CGGGATCGTCCCGCCCGGCACCGATGCATCCGGAAAGCCGCACTACATCCGCATGTATTCGGTGGCCAGCCCGCGCGACG
GCGAGCGGCCGGGTTATAACAACCTCGCGCTGACCGTAAAGCGCGTGGACACCGATCACGATGGCAACCCAGTGCGCGGC
GTGGCGTCGAACTTCCTGTGCGACCTGGCCAAGGGTGACCCTGTGCAGGTGGTCGGGCCGTTCGGCAGCACATTCCTGAT
GCCGAACCACCGCGAAGCGAGCGTGATGATGATCTGCACCGGTACCGGATCCGCGCCGATGCGGGCGATGACCGAGCGTA
TGCGCCGCAACATGGACCACTTCAGCGGTCGCCGGCTGCTGTTCTTCGGCGCCCGCAATCGGCGGGAACTGCCCTACTTT
GGCCCGCTGCTGAAGCTACCCAAGGACTTCCTGGACATCCACTTCGCGTTCTCGCGCGATCCGGAGGTACCGCGCCGGTA
TGTGCAGGACGCCATTCGCGAGGCCTCGGCCCAGGTGGCCGCGTTGCTGGCGGACCCGCATGGTCATATCTACATCTGCG
GACTCAAGGGGATGGAGGAAGGCGTGCTGGATGCATTCGCCGAGGTCTGCGCCACGTCGGGTCAGTCGTGGCAGGACATC
GAACCGCGCCTGCGTGCCGAAGGTCGCCTGCATATCGAAACGTATTAG

Upstream 100 bases:

>100_bases
CCAACTGGATCGCGCCGCCGGCTGGTGGCATCAACCGCCAGCCGATTGATTTCGAGTACGTTCGTTTCAACTGACGACCT
ATCCATCGAGGCTGACGATC

Downstream 100 bases:

>100_bases
GAACAGGATCGGGAAACGGAATCGCCACAGGAATCAACACCATTTGCAGCTTCCCTTCTGAAACTGGGTGCCTATAATGT
TTCTCAAGAAACAGGAGCAT

Product: benzoyl-CoA oxygenase component A

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit A; Benzoyl-CoA dioxygenase reductase component [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MGAADIIKQHLIDPEICIRCNTCEDTCPIDAITHDDRNYVVKADVCNACNACLSPCPTGAIDNWRTMLKGQAYTIEAQLT
WDELPEEVPLPEAEIEAAAAAGQVIEEASRGSKSVAVQEVEASRHTSSRAPWSAAHPYVNLHGVREPVTATVAGNHRLTA
EDASSDIHHIVLDFGNHFFPVLEGQAIGIVPPGTDASGKPHYIRMYSVASPRDGERPGYNNLALTVKRVDTDHDGNPVRG
VASNFLCDLAKGDPVQVVGPFGSTFLMPNHREASVMMICTGTGSAPMRAMTERMRRNMDHFSGRRLLFFGARNRRELPYF
GPLLKLPKDFLDIHFAFSRDPEVPRRYVQDAIREASAQVAALLADPHGHIYICGLKGMEEGVLDAFAEVCATSGQSWQDI
EPRLRAEGRLHIETY

Sequences:

>Translated_415_residues
MGAADIIKQHLIDPEICIRCNTCEDTCPIDAITHDDRNYVVKADVCNACNACLSPCPTGAIDNWRTMLKGQAYTIEAQLT
WDELPEEVPLPEAEIEAAAAAGQVIEEASRGSKSVAVQEVEASRHTSSRAPWSAAHPYVNLHGVREPVTATVAGNHRLTA
EDASSDIHHIVLDFGNHFFPVLEGQAIGIVPPGTDASGKPHYIRMYSVASPRDGERPGYNNLALTVKRVDTDHDGNPVRG
VASNFLCDLAKGDPVQVVGPFGSTFLMPNHREASVMMICTGTGSAPMRAMTERMRRNMDHFSGRRLLFFGARNRRELPYF
GPLLKLPKDFLDIHFAFSRDPEVPRRYVQDAIREASAQVAALLADPHGHIYICGLKGMEEGVLDAFAEVCATSGQSWQDI
EPRLRAEGRLHIETY
>Mature_414_residues
GAADIIKQHLIDPEICIRCNTCEDTCPIDAITHDDRNYVVKADVCNACNACLSPCPTGAIDNWRTMLKGQAYTIEAQLTW
DELPEEVPLPEAEIEAAAAAGQVIEEASRGSKSVAVQEVEASRHTSSRAPWSAAHPYVNLHGVREPVTATVAGNHRLTAE
DASSDIHHIVLDFGNHFFPVLEGQAIGIVPPGTDASGKPHYIRMYSVASPRDGERPGYNNLALTVKRVDTDHDGNPVRGV
ASNFLCDLAKGDPVQVVGPFGSTFLMPNHREASVMMICTGTGSAPMRAMTERMRRNMDHFSGRRLLFFGARNRRELPYFG
PLLKLPKDFLDIHFAFSRDPEVPRRYVQDAIREASAQVAALLADPHGHIYICGLKGMEEGVLDAFAEVCATSGQSWQDIE
PRLRAEGRLHIETY

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxA acts as a reductase that uses NADPH to reduce the oxygenase component BoxB. BoxAB does not act on NADH

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=224, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI40254422, Length=209, Percent_Identity=29.1866028708134, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI10835173, Length=224, Percent_Identity=26.7857142857143, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789123, Length=226, Percent_Identity=31.858407079646, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321143, Length=178, Percent_Identity=32.5842696629214, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI78706872, Length=223, Percent_Identity=31.390134529148, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583543, Length=223, Percent_Identity=31.390134529148, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78706876, Length=223, Percent_Identity=31.390134529148, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017634
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012146
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00037 Fer4; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 45563; Mature: 45432

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAADIIKQHLIDPEICIRCNTCEDTCPIDAITHDDRNYVVKADVCNACNACLSPCPTGA
CCHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHCCCCCCC
IDNWRTMLKGQAYTIEAQLTWDELPEEVPLPEAEIEAAAAAGQVIEEASRGSKSVAVQEV
CHHHHHHHCCCEEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
EASRHTSSRAPWSAAHPYVNLHGVREPVTATVAGNHRLTAEDASSDIHHIVLDFGNHFFP
HHHHCCCCCCCCCCCCCEEEECCCCCCEEEEEECCEEEECCCCCCCCEEHEECCCCCEEE
VLEGQAIGIVPPGTDASGKPHYIRMYSVASPRDGERPGYNNLALTVKRVDTDHDGNPVRG
EECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCHH
VASNFLCDLAKGDPVQVVGPFGSTFLMPNHREASVMMICTGTGSAPMRAMTERMRRNMDH
HHHHHHHCCCCCCCEEEECCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
FSGRRLLFFGARNRRELPYFGPLLKLPKDFLDIHFAFSRDPEVPRRYVQDAIREASAQVA
HCCCEEEEEECCCCCCCCCCCHHHHCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHEE
ALLADPHGHIYICGLKGMEEGVLDAFAEVCATSGQSWQDIEPRLRAEGRLHIETY
EEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEEEEC
>Mature Secondary Structure 
GAADIIKQHLIDPEICIRCNTCEDTCPIDAITHDDRNYVVKADVCNACNACLSPCPTGA
CHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHCCCCCCC
IDNWRTMLKGQAYTIEAQLTWDELPEEVPLPEAEIEAAAAAGQVIEEASRGSKSVAVQEV
CHHHHHHHCCCEEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
EASRHTSSRAPWSAAHPYVNLHGVREPVTATVAGNHRLTAEDASSDIHHIVLDFGNHFFP
HHHHCCCCCCCCCCCCCEEEECCCCCCEEEEEECCEEEECCCCCCCCEEHEECCCCCEEE
VLEGQAIGIVPPGTDASGKPHYIRMYSVASPRDGERPGYNNLALTVKRVDTDHDGNPVRG
EECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCHH
VASNFLCDLAKGDPVQVVGPFGSTFLMPNHREASVMMICTGTGSAPMRAMTERMRRNMDH
HHHHHHHCCCCCCCEEEECCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
FSGRRLLFFGARNRRELPYFGPLLKLPKDFLDIHFAFSRDPEVPRRYVQDAIREASAQVA
HCCCEEEEEECCCCCCCCCCCHHHHCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHEE
ALLADPHGHIYICGLKGMEEGVLDAFAEVCATSGQSWQDIEPRLRAEGRLHIETY
EEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]