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The map label for this gene is lpdA [H]

Identifier: 94310144

GI number: 94310144

Start: 1317732

End: 1319510

Strand: Direct

Name: lpdA [H]

Synonym: Rmet_1199

Alternate gene names: 94310144

Gene position: 1317732-1319510 (Clockwise)

Preceding gene: 94310143

Following gene: 94310146

Centisome position: 33.55

GC content: 64.64

Gene sequence:

>1779_bases
ATGAGTGTGATCGAAGTCAAGGTGCCGGATATCGGCGATTTCGACGCGGTGGAAGTGATCGAGGTGCTGGTCAAGGCTGG
CGACACGGTCGAGGAAGAACAGTCGCTGATCGTGCTGGAGTCCGACAAGGCCAGCATGGAAGTGCCGTCGTCGGCCGCCG
GCAAGGTCGTGGACGTCAAGGTCAAGGTGGGCGACAAGGTCGCGAAGGGCACGCTGATCTGCACCGTCGAAGGTGGCGCG
GCGGCGGCTCCTGCCCCGGCGCCGGCGGCGGCGCCCAAGCCCGCGGCTGCGCCTGCTCCGGCGCCGGCAGCGGCTCCGGC
GGCCGCCACGCATGCCGGTGGCGCCGACATCCAGTGCGAAATGCTGGTGCTGGGCGCAGGCCCCGGGGGTTACTCGGCGG
CTTTCCGCTCGGCGGACCTCGGCATGAACACCGTTCTGGTCGAGCGCTATGGCACCCTGGGCGGCGTTTGCCTGAACGTG
GGCTGCATCCCGTCGAAGGCGCTGCTGCACAACGCGGCGGTGATCGACGAGGCCAAGGCACTGGCCGCGCACGGCATCCT
GTTCGGCGAAGCCAAGATCGATCTGGATGGCCTGCGGCACTACAAGGAAAGCGTGGTCGGCAAGCTGACCGGCGGTCTGT
CGGGAATGGCGAAGGCCCGCAAGGTGCAGGTGGTGCGCGGCATCGGCACGTTCCTGGATCCGCATCATCTGGAAGTGCAG
GAAACGGAAGGCGACAGCAAGGCCACCAATGGCAAGAAGACCGTGATCCGCTTCGAAAAGGCGATCATCGCGGCTGGCAG
CCAGGCCGTGAAGCTGCCGTTCGTCCCTGAGGATCCGCGCATTTTCGATTCGACGGGCGCGCTGGAACTGCGCGACATCC
CGAACAAGATGCTCGTGATTGGCGGCGGCATCATCGGCCTGGAAATGGCCACTGTCTACAGCACGCTCGGCGCGCGACTC
GATGTCGTGGAAATGCTCGACGGCCTGATGCAGGGCGCTGACCGCGATCTGGTCAAGGTCTGGGACAAGGTGAACAAGCA
TCGCTTCGATAACGTGATGCTGAAGACCAAGACTGTCGGCGTGGAAGCGAAGCCGGATGGCATCTACGTGAAGTTCGAGG
GCGAGTCCGCGCCGGCCGAGCCGCAGCGTTACGACGCCGTGCTGGTGTCGGTGGGCCGTTCCCCGAACGGCAAGAAGATC
GGTGCCGAGAAGGCCGGTGTAGCAGTGACCGACCGTGGCTTCATCGATGTCGACAAGCAGATGCGCACCAATGTGCCGCA
CATCTACGCGATCGGCGATATCGTCGGCCAGCCGATGCTTGCACACAAGGCGGTGCATGAGGCACATGTGGCCGCCGAAG
CCGCGCATGGCGAGAAGGCCTACTTCGACGCGAAGCAGATCCCGTCCGTGGCGTTCACCGATCCGGAAGTGGCATGGGCC
GGCAAGACCGAAGACCAGTGCAAGGCCGAAGGCATCAAGTACAGCAAGGGTGTGTTCCCGTGGGCCGCTTCGGGCCGCGC
GATCGCCAATGGGCGTGACGAAGGCTTCACCAAGCTGATTTTCGACGAGGAAACCCATCGCATCATCGGTGGCGGTATCG
TCGGCACCCACGCGGGCGACCTGATCAGCGAAGTCTGCCTGGCCATCGAGATGGGCGCGGATGCCGTGGATATTGGCAAG
ACGATTCACCCGCACCCGACCTTGGGCGAGTCGATTGGCATGGCTGCGGAAATCTACGAAGGTGTGTGCACCGACGTGCC
GCCGCCGCGCAAGCGCTGA

Upstream 100 bases:

>100_bases
ACCGATCGACGTGCACACGGTACGGCTGCAAGTGGCAACCAACAAAGACGGCGCGGCCTGATCCCAAGGCGGGCAGGGCG
CGCATCCCAGGAGGAAGAAC

Downstream 100 bases:

>100_bases
TTGCTGGCGCCGTAGTGAGAGAACCCGCCGATTCGGCGGGTTTTTTTGTCTCGACTCTCCTGCGAGCACTGGGGGGAGAA
CGCGACGAAAAAAAGCCCGG

Product: Dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MSVIEVKVPDIGDFDAVEVIEVLVKAGDTVEEEQSLIVLESDKASMEVPSSAAGKVVDVKVKVGDKVAKGTLICTVEGGA
AAAPAPAPAAAPKPAAAPAPAPAAAPAAATHAGGADIQCEMLVLGAGPGGYSAAFRSADLGMNTVLVERYGTLGGVCLNV
GCIPSKALLHNAAVIDEAKALAAHGILFGEAKIDLDGLRHYKESVVGKLTGGLSGMAKARKVQVVRGIGTFLDPHHLEVQ
ETEGDSKATNGKKTVIRFEKAIIAAGSQAVKLPFVPEDPRIFDSTGALELRDIPNKMLVIGGGIIGLEMATVYSTLGARL
DVVEMLDGLMQGADRDLVKVWDKVNKHRFDNVMLKTKTVGVEAKPDGIYVKFEGESAPAEPQRYDAVLVSVGRSPNGKKI
GAEKAGVAVTDRGFIDVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVAWA
GKTEDQCKAEGIKYSKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAGDLISEVCLAIEMGADAVDIGK
TIHPHPTLGESIGMAAEIYEGVCTDVPPPRKR

Sequences:

>Translated_592_residues
MSVIEVKVPDIGDFDAVEVIEVLVKAGDTVEEEQSLIVLESDKASMEVPSSAAGKVVDVKVKVGDKVAKGTLICTVEGGA
AAAPAPAPAAAPKPAAAPAPAPAAAPAAATHAGGADIQCEMLVLGAGPGGYSAAFRSADLGMNTVLVERYGTLGGVCLNV
GCIPSKALLHNAAVIDEAKALAAHGILFGEAKIDLDGLRHYKESVVGKLTGGLSGMAKARKVQVVRGIGTFLDPHHLEVQ
ETEGDSKATNGKKTVIRFEKAIIAAGSQAVKLPFVPEDPRIFDSTGALELRDIPNKMLVIGGGIIGLEMATVYSTLGARL
DVVEMLDGLMQGADRDLVKVWDKVNKHRFDNVMLKTKTVGVEAKPDGIYVKFEGESAPAEPQRYDAVLVSVGRSPNGKKI
GAEKAGVAVTDRGFIDVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVAWA
GKTEDQCKAEGIKYSKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAGDLISEVCLAIEMGADAVDIGK
TIHPHPTLGESIGMAAEIYEGVCTDVPPPRKR
>Mature_591_residues
SVIEVKVPDIGDFDAVEVIEVLVKAGDTVEEEQSLIVLESDKASMEVPSSAAGKVVDVKVKVGDKVAKGTLICTVEGGAA
AAPAPAPAAAPKPAAAPAPAPAAAPAAATHAGGADIQCEMLVLGAGPGGYSAAFRSADLGMNTVLVERYGTLGGVCLNVG
CIPSKALLHNAAVIDEAKALAAHGILFGEAKIDLDGLRHYKESVVGKLTGGLSGMAKARKVQVVRGIGTFLDPHHLEVQE
TEGDSKATNGKKTVIRFEKAIIAAGSQAVKLPFVPEDPRIFDSTGALELRDIPNKMLVIGGGIIGLEMATVYSTLGARLD
VVEMLDGLMQGADRDLVKVWDKVNKHRFDNVMLKTKTVGVEAKPDGIYVKFEGESAPAEPQRYDAVLVSVGRSPNGKKIG
AEKAGVAVTDRGFIDVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVAWAG
KTEDQCKAEGIKYSKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAGDLISEVCLAIEMGADAVDIGKT
IHPHPTLGESIGMAAEIYEGVCTDVPPPRKR

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=40.2597402597403, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI50301238, Length=480, Percent_Identity=26.0416666666667, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI148277071, Length=451, Percent_Identity=28.159645232816, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI33519430, Length=451, Percent_Identity=28.159645232816, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI33519428, Length=451, Percent_Identity=28.159645232816, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI33519426, Length=451, Percent_Identity=28.159645232816, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI148277065, Length=451, Percent_Identity=28.159645232816, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI291045266, Length=446, Percent_Identity=28.2511210762332, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI291045268, Length=436, Percent_Identity=26.8348623853211, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI22035672, Length=452, Percent_Identity=28.5398230088496, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1786307, Length=478, Percent_Identity=64.2259414225941, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI87082354, Length=470, Percent_Identity=27.6595744680851, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI87081717, Length=462, Percent_Identity=25.974025974026, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789915, Length=452, Percent_Identity=27.8761061946903, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI1786305, Length=73, Percent_Identity=63.013698630137, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=40.0437636761488, Blast_Score=326, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17557007, Length=493, Percent_Identity=26.5720081135903, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=452, Percent_Identity=23.4513274336283, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=452, Percent_Identity=23.4513274336283, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=455, Percent_Identity=25.2747252747253, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=459, Percent_Identity=39.6514161220044, Blast_Score=303, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=28.7211740041929, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=475, Percent_Identity=26.1052631578947, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=40.6521739130435, Blast_Score=337, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24640551, Length=520, Percent_Identity=27.8846153846154, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640549, Length=480, Percent_Identity=27.7083333333333, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24640553, Length=497, Percent_Identity=27.364185110664, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI17737741, Length=486, Percent_Identity=25.1028806584362, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 61833; Mature: 61701

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIEVKVPDIGDFDAVEVIEVLVKAGDTVEEEQSLIVLESDKASMEVPSSAAGKVVDVK
CCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VKVGDKVAKGTLICTVEGGAAAAPAPAPAAAPKPAAAPAPAPAAAPAAATHAGGADIQCE
EEECCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
MLVLGAGPGGYSAAFRSADLGMNTVLVERYGTLGGVCLNVGCIPSKALLHNAAVIDEAKA
EEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
LAAHGILFGEAKIDLDGLRHYKESVVGKLTGGLSGMAKARKVQVVRGIGTFLDPHHLEVQ
HHHCCEEEECEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
ETEGDSKATNGKKTVIRFEKAIIAAGSQAVKLPFVPEDPRIFDSTGALELRDIPNKMLVI
ECCCCCCCCCCCEEEEEEHHHHHHCCCCEEECCCCCCCCCEECCCCCEEEECCCCEEEEE
GGGIIGLEMATVYSTLGARLDVVEMLDGLMQGADRDLVKVWDKVNKHRFDNVMLKTKTVG
ECCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEEC
VEAKPDGIYVKFEGESAPAEPQRYDAVLVSVGRSPNGKKIGAEKAGVAVTDRGFIDVDKQ
EEECCCEEEEEECCCCCCCCCHHHCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECHH
MRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVAWA
HHCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCCCHHEECHHHCCCEEECCCCEEEC
GKTEDQCKAEGIKYSKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAGD
CCCHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCEEEECCEECCCHHH
LISEVCLAIEMGADAVDIGKTIHPHPTLGESIGMAAEIYEGVCTDVPPPRKR
HHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVIEVKVPDIGDFDAVEVIEVLVKAGDTVEEEQSLIVLESDKASMEVPSSAAGKVVDVK
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VKVGDKVAKGTLICTVEGGAAAAPAPAPAAAPKPAAAPAPAPAAAPAAATHAGGADIQCE
EEECCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
MLVLGAGPGGYSAAFRSADLGMNTVLVERYGTLGGVCLNVGCIPSKALLHNAAVIDEAKA
EEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
LAAHGILFGEAKIDLDGLRHYKESVVGKLTGGLSGMAKARKVQVVRGIGTFLDPHHLEVQ
HHHCCEEEECEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
ETEGDSKATNGKKTVIRFEKAIIAAGSQAVKLPFVPEDPRIFDSTGALELRDIPNKMLVI
ECCCCCCCCCCCEEEEEEHHHHHHCCCCEEECCCCCCCCCEECCCCCEEEECCCCEEEEE
GGGIIGLEMATVYSTLGARLDVVEMLDGLMQGADRDLVKVWDKVNKHRFDNVMLKTKTVG
ECCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEEC
VEAKPDGIYVKFEGESAPAEPQRYDAVLVSVGRSPNGKKIGAEKAGVAVTDRGFIDVDKQ
EEECCCEEEEEECCCCCCCCCHHHCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECHH
MRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVAWA
HHCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCCCHHEECHHHCCCEEECCCCEEEC
GKTEDQCKAEGIKYSKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAGD
CCCHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCEEEECCEECCCHHH
LISEVCLAIEMGADAVDIGKTIHPHPTLGESIGMAAEIYEGVCTDVPPPRKR
HHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]