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The map label for this gene is xthA2 [H]

Identifier: 94310134

GI number: 94310134

Start: 1303315

End: 1304112

Strand: Reverse

Name: xthA2 [H]

Synonym: Rmet_1189

Alternate gene names: 94310134

Gene position: 1304112-1303315 (Counterclockwise)

Preceding gene: 94310135

Following gene: 94310133

Centisome position: 33.2

GC content: 62.53

Gene sequence:

>798_bases
ATGAGAATCGCAAGCTGGAACGTCAACTCCCTCAAGGTACGTCTGCCGCAGGTGCTGCAATGGCTAGGCGCGCAGGATGC
CGCGGGCACGCCGATCGATGCGCTGTGCCTGCAGGAACTAAAGCTGCCCGACGACAAGTACCCGCTGGCCGAACTCCAAG
CGGCCGGCTTCCATAGCATCTTCACAGGTCAGAAAACCTACAACGGTGTGGCGATCCTGGCACGCAACGCGGCCATGCCC
GTGCCGACTGACGTGGTGCGCAACATTCCCGGCTATGAGGATGCGCAGCAACGCGTGATCGCCGGCACGTACGGCGACGT
ACGTCTGATTAGCGCGTACTTCCCGAATGGCCAGGCGCTGGACTCGGACAAGTTCGTCTACAAGCTCGACTGGCTCAAGG
CCATGATCGAGTGGCTGCGCGCCGAAATGACCCGGTATCCGAAACTCGCGCTGCTGGGAGACTTCAACATCGCCCCGGAA
GACCGCGACGTCCACGACCCGGCCAAGTGGGAGGGCCAGAACCTGGTCTCGCCGCAGGAGCGCGACGCGTTCCAGACATT
GATCGGCCTGGGTCTGGTTGACTCCTTCCGCATGTTCGAACAGCCCGAGAAAATGTTCTCGTGGTGGGATTACCGAATGA
TGGGTTTCCGCCGCAATGCCGGGTTGCGTATCGACCATATCCTGCTTTCGGCCGACCTGGCGCGGCAGTGCGCGACGTGT
GTCATCGACAAGGAGCCGCGCCGCTGGGAGCAGCCGTCTGACCACACCCCTGTCGTGGCCACGCTCACGCTCGGCTAG

Upstream 100 bases:

>100_bases
AAATCGCCTACGCGATCCTGGACCAGCGCGGACTGCTTGGCGGCATGGACGCCATCGGCGACGCGCACGGCGACAACGAC
AATCCAAACAAAAACGAGAT

Downstream 100 bases:

>100_bases
CCGATGCGTGCGAATCTGGCCCTGACGCGCTGGTTTCCCTGGCTCCCGCGCGTCACGCCGCTGACGCTGCGCGCCGATCT
GGTTGCCGGACTGCTTGGCG

Product: exodeoxyribonuclease III (xth)

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRIASWNVNSLKVRLPQVLQWLGAQDAAGTPIDALCLQELKLPDDKYPLAELQAAGFHSIFTGQKTYNGVAILARNAAMP
VPTDVVRNIPGYEDAQQRVIAGTYGDVRLISAYFPNGQALDSDKFVYKLDWLKAMIEWLRAEMTRYPKLALLGDFNIAPE
DRDVHDPAKWEGQNLVSPQERDAFQTLIGLGLVDSFRMFEQPEKMFSWWDYRMMGFRRNAGLRIDHILLSADLARQCATC
VIDKEPRRWEQPSDHTPVVATLTLG

Sequences:

>Translated_265_residues
MRIASWNVNSLKVRLPQVLQWLGAQDAAGTPIDALCLQELKLPDDKYPLAELQAAGFHSIFTGQKTYNGVAILARNAAMP
VPTDVVRNIPGYEDAQQRVIAGTYGDVRLISAYFPNGQALDSDKFVYKLDWLKAMIEWLRAEMTRYPKLALLGDFNIAPE
DRDVHDPAKWEGQNLVSPQERDAFQTLIGLGLVDSFRMFEQPEKMFSWWDYRMMGFRRNAGLRIDHILLSADLARQCATC
VIDKEPRRWEQPSDHTPVVATLTLG
>Mature_265_residues
MRIASWNVNSLKVRLPQVLQWLGAQDAAGTPIDALCLQELKLPDDKYPLAELQAAGFHSIFTGQKTYNGVAILARNAAMP
VPTDVVRNIPGYEDAQQRVIAGTYGDVRLISAYFPNGQALDSDKFVYKLDWLKAMIEWLRAEMTRYPKLALLGDFNIAPE
DRDVHDPAKWEGQNLVSPQERDAFQTLIGLGLVDSFRMFEQPEKMFSWWDYRMMGFRRNAGLRIDHILLSADLARQCATC
VIDKEPRRWEQPSDHTPVVATLTLG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=273, Percent_Identity=30.7692307692308, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI18375503, Length=273, Percent_Identity=30.7692307692308, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI18375501, Length=273, Percent_Identity=30.7692307692308, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1788046, Length=275, Percent_Identity=37.0909090909091, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=199, Percent_Identity=27.1356783919598, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221330655, Length=274, Percent_Identity=27.3722627737226, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17136678, Length=274, Percent_Identity=27.3722627737226, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30037; Mature: 30037

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIASWNVNSLKVRLPQVLQWLGAQDAAGTPIDALCLQELKLPDDKYPLAELQAAGFHSI
CEEECCCCCHHEEHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCHHH
FTGQKTYNGVAILARNAAMPVPTDVVRNIPGYEDAQQRVIAGTYGDVRLISAYFPNGQAL
HCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCHHCEEEECCCCCEEEEEEECCCCCCC
DSDKFVYKLDWLKAMIEWLRAEMTRYPKLALLGDFNIAPEDRDVHDPAKWEGQNLVSPQE
CCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHH
RDAFQTLIGLGLVDSFRMFEQPEKMFSWWDYRMMGFRRNAGLRIDHILLSADLARQCATC
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHH
VIDKEPRRWEQPSDHTPVVATLTLG
HCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRIASWNVNSLKVRLPQVLQWLGAQDAAGTPIDALCLQELKLPDDKYPLAELQAAGFHSI
CEEECCCCCHHEEHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCHHH
FTGQKTYNGVAILARNAAMPVPTDVVRNIPGYEDAQQRVIAGTYGDVRLISAYFPNGQAL
HCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCHHCEEEECCCCCEEEEEEECCCCCCC
DSDKFVYKLDWLKAMIEWLRAEMTRYPKLALLGDFNIAPEDRDVHDPAKWEGQNLVSPQE
CCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHH
RDAFQTLIGLGLVDSFRMFEQPEKMFSWWDYRMMGFRRNAGLRIDHILLSADLARQCATC
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHH
VIDKEPRRWEQPSDHTPVVATLTLG
HCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]