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The map label for this gene is metY [H]

Identifier: 94310079

GI number: 94310079

Start: 1240313

End: 1241665

Strand: Reverse

Name: metY [H]

Synonym: Rmet_1134

Alternate gene names: 94310079

Gene position: 1241665-1240313 (Counterclockwise)

Preceding gene: 94310085

Following gene: 94310078

Centisome position: 31.61

GC content: 67.92

Gene sequence:

>1353_bases
ATGCCGGACGGAGACAACCACCCATGGCTCGGCAAACCGAGCCTTTTCACCATGTCCGGTACCCGCTTTGACACGCTGGC
CCTGCACGCCGGCGCCGCGCCCGACCCCACCACCGGGGCCCGCGCCACACCGATCCATCTGACCACATCCTTCGTATTCC
GCGACAGCGAGCACGCCGCATCGCTGTTCAATATGGAACGCGCCGGACACGTCTATTCGCGCATCAGCAACCCCACGGTC
GCCGTGTTCGAGGAGCGCGTGGCCGCTCTGGAGAACGGCGCGGGCGCCATCGCCACGGCCTCGGGCCAGGCCGCCCTGCA
CCTGGCCGTGGCCACGCTGATGGGCGCCGGCTCCCATATCGTGGCGTCCACCGCGCTCTATGGCGGGTCTCACAACCTGC
TGCACTACACGCTGCGCCGCTTCGGCATCGAGACCACCTTCGTCAATCCGCGCGACATCGACGCCTGGCGCGCCGCGATC
CGCCCCGAGACGAAGCTGCTCTTCGGTGAAACACTGGGCAACCCCGGCCTGGACGTGCTCGACATCGCCACCGTGGCCCA
GATCGGCCAGGAACACGGCATTCCGCTGCTGGTGGATTCGACGTTCACCACGCCCTACCTGCTGCGCCCGTTCGACCACG
GCGCCGGGCTGCTCTACCACTCGGCCACCAAGTTCCTGGGCGGCCATGGCACGACGATCGGCGGCGTGCTTTCAGAAGGC
GGCACGTTCGATTTCCAGGCCTCGGGCCGCTTCCCGGAACTGTCCGAGCCGTACGAGGGCTTCCACAACATGGTGTTCAC
CGAGGAAAGCACGGTAGCGCCATTTCTGCTGCGCGCGCGGCGCGAGGGCCTGCGCGACTTCGGCGCCTGCATGAATCCGA
TGGCTGCCTGGCAGTTGCTGCAGGGCATCGAGACGCTGCCGCTGCGCATGAGCCGCCACGTCGACAACACGCGGCGCGTG
GTGCAGTTCCTGGCCAGCCACCCGATGGTCGAGTCGGTGGCGTATCCGGAGCTCGAATCTCACCCGGACTACGAACTGGC
CAAGCGCCTGCTGCCGCGCGGCTGCGGCGCGGTGTTCAGCTTCAACCTCAAGGCCGACCGCACCGCCGGCCAGCGCTTCA
TCGAAAGCCTGTCGCTGTTCTCGCACCTGGCCAACGTGGGCGATGCGCGCTCCCTGGTGATCCATCCGGCGTCCACCACC
CACTTCCGCATGGACGCCGCGGCGCTGCGCGCGGCCGGCATCGGCGAAGGCACGATCCGCCTGTCGATCGGCCTGGAGGA
TCCGGACGACCTGATCGACGACCTGAAACGCGGCCTCAAGGCCGCCGAGAAGGCCATGGGAGGGAAAGCCTGA

Upstream 100 bases:

>100_bases
GATCCTAGCCCAAACCGCCGTCTCAAGTAAAGCGAGCGCGGCTTCCAGCGCCCGTCCCGCCGTGGATTCCCGGGTTACGC
ATGTGGCGTATGGTGCTACG

Downstream 100 bases:

>100_bases
TGGAACTGAAACTCTCCGGCCACAAGGCCTACGCCTACACCGGCGGCAAGCCGTTCGACCCCGCCCTGCCCTGCGCCGTC
TTCGTGCACGGTGCGCAGAA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPDGDNHPWLGKPSLFTMSGTRFDTLALHAGAAPDPTTGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISNPTV
AVFEERVAALENGAGAIATASGQAALHLAVATLMGAGSHIVASTALYGGSHNLLHYTLRRFGIETTFVNPRDIDAWRAAI
RPETKLLFGETLGNPGLDVLDIATVAQIGQEHGIPLLVDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLSEG
GTFDFQASGRFPELSEPYEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGIETLPLRMSRHVDNTRRV
VQFLASHPMVESVAYPELESHPDYELAKRLLPRGCGAVFSFNLKADRTAGQRFIESLSLFSHLANVGDARSLVIHPASTT
HFRMDAAALRAAGIGEGTIRLSIGLEDPDDLIDDLKRGLKAAEKAMGGKA

Sequences:

>Translated_450_residues
MPDGDNHPWLGKPSLFTMSGTRFDTLALHAGAAPDPTTGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISNPTV
AVFEERVAALENGAGAIATASGQAALHLAVATLMGAGSHIVASTALYGGSHNLLHYTLRRFGIETTFVNPRDIDAWRAAI
RPETKLLFGETLGNPGLDVLDIATVAQIGQEHGIPLLVDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLSEG
GTFDFQASGRFPELSEPYEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGIETLPLRMSRHVDNTRRV
VQFLASHPMVESVAYPELESHPDYELAKRLLPRGCGAVFSFNLKADRTAGQRFIESLSLFSHLANVGDARSLVIHPASTT
HFRMDAAALRAAGIGEGTIRLSIGLEDPDDLIDDLKRGLKAAEKAMGGKA
>Mature_449_residues
PDGDNHPWLGKPSLFTMSGTRFDTLALHAGAAPDPTTGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISNPTVA
VFEERVAALENGAGAIATASGQAALHLAVATLMGAGSHIVASTALYGGSHNLLHYTLRRFGIETTFVNPRDIDAWRAAIR
PETKLLFGETLGNPGLDVLDIATVAQIGQEHGIPLLVDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLSEGG
TFDFQASGRFPELSEPYEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGIETLPLRMSRHVDNTRRVV
QFLASHPMVESVAYPELESHPDYELAKRLLPRGCGAVFSFNLKADRTAGQRFIESLSLFSHLANVGDARSLVIHPASTTH
FRMDAAALRAAGIGEGTIRLSIGLEDPDDLIDDLKRGLKAAEKAMGGKA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=427, Percent_Identity=35.5971896955504, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI299473759, Length=427, Percent_Identity=33.0210772833724, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI25453487, Length=427, Percent_Identity=30.6791569086651, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=34.5238095238095, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1789383, Length=433, Percent_Identity=26.7898383371824, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71980708, Length=432, Percent_Identity=29.1666666666667, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=425, Percent_Identity=31.0588235294118, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=31.1320754716981, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=42.1658986175115, Blast_Score=306, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6319307, Length=427, Percent_Identity=32.7868852459016, Blast_Score=189, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6321170, Length=357, Percent_Identity=24.9299719887955, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321254, Length=452, Percent_Identity=25.8849557522124, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=26.3403263403263, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922554, Length=426, Percent_Identity=34.037558685446, Blast_Score=224, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 48506; Mature: 48374

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDGDNHPWLGKPSLFTMSGTRFDTLALHAGAAPDPTTGARATPIHLTTSFVFRDSEHAA
CCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCHHHH
SLFNMERAGHVYSRISNPTVAVFEERVAALENGAGAIATASGQAALHLAVATLMGAGSHI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHH
VASTALYGGSHNLLHYTLRRFGIETTFVNPRDIDAWRAAIRPETKLLFGETLGNPGLDVL
HHHHHEECCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHCCCCCEEEEHHHCCCCCCHHH
DIATVAQIGQEHGIPLLVDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLSEG
HHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCC
GTFDFQASGRFPELSEPYEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLL
CCEEEECCCCCCCCCCHHHHHHCEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
QGIETLPLRMSRHVDNTRRVVQFLASHPMVESVAYPELESHPDYELAKRLLPRGCGAVFS
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEE
FNLKADRTAGQRFIESLSLFSHLANVGDARSLVIHPASTTHFRMDAAALRAAGIGEGTIR
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCEEE
LSIGLEDPDDLIDDLKRGLKAAEKAMGGKA
EEECCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PDGDNHPWLGKPSLFTMSGTRFDTLALHAGAAPDPTTGARATPIHLTTSFVFRDSEHAA
CCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCHHHH
SLFNMERAGHVYSRISNPTVAVFEERVAALENGAGAIATASGQAALHLAVATLMGAGSHI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHH
VASTALYGGSHNLLHYTLRRFGIETTFVNPRDIDAWRAAIRPETKLLFGETLGNPGLDVL
HHHHHEECCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHCCCCCEEEEHHHCCCCCCHHH
DIATVAQIGQEHGIPLLVDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLSEG
HHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCC
GTFDFQASGRFPELSEPYEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLL
CCEEEECCCCCCCCCCHHHHHHCEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
QGIETLPLRMSRHVDNTRRVVQFLASHPMVESVAYPELESHPDYELAKRLLPRGCGAVFS
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEE
FNLKADRTAGQRFIESLSLFSHLANVGDARSLVIHPASTTHFRMDAAALRAAGIGEGTIR
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCEEE
LSIGLEDPDDLIDDLKRGLKAAEKAMGGKA
EEECCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]