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The map label for this gene is recJ [H]

Identifier: 94309983

GI number: 94309983

Start: 1127253

End: 1128953

Strand: Reverse

Name: recJ [H]

Synonym: Rmet_1038

Alternate gene names: 94309983

Gene position: 1128953-1127253 (Counterclockwise)

Preceding gene: 94309984

Following gene: 94309981

Centisome position: 28.74

GC content: 66.08

Gene sequence:

>1701_bases
ATGACCCGGATTGCCATCCGCTCCTTCTCCGACGAACACGCAAAGGCCCTGGCGGCCTCGGGTCTGCATCCGACGCTGGC
GCGCATCCTGTCCGCGCGCGGCGTAGCCCAACCCTCCGAACTGGCCACGGACCTGCCCGAACTGGCGCCGCCGGCCAGCA
TGAAAGGCATCGGCCATGCGGCCGAGTACCTGGCCGACGCGATTGCCGCGAATCGCAAGCTGCTGATCGTGGCTGACTAC
GATTGCGATGGCGCCACGGCCTGCGCGGTGGGCGTGCGCGGGCTGCGCATGCTGGGCGCGCGCGTCGACTACATCGTGCC
GAACCGCTTCGAGTACGGCTACGGGCTGACGCCGGAGATCGTCGAACTGGCGGCCCGGCAACATCCTGACGTGATCGTCA
CGGTCGACAACGGCATCGCCAGCGTCGACGGCGTGGCGGCCGCCAACGCACGCGGCATCGACGTCGTGGTGACCGACCAC
CACCTGCCCGGCGAGACGTTGCCCGAGGCTGCGGTGATCGTGAACCCGAACCAACCGGGATGCGAGTTCCCGAGCAAGAA
CCTGGCGGGTGTCGGGGTGATGTTCTATGTGCTGCTGGCCCTGCGCGCCGAGTTGCGCCGCCGCGGCGTGTACACCCAGG
CCACCCAGCCGCCGCTGCAAACCCTGCTGGACCTCGTGGCGCTGGGCACGGTGGCCGACGTGGTCAAACTGGACACGAAC
AACCGGATTCTGGTGGCCCAGGGGCTGAAGCGCATGCGCGCAGGACGGATGCATCCCGGCGTGGCGGCACTGTTCCGTGC
CGCCGGCCGCGAAGCCTCGCGCGCAAACACGTTCGACCTCGGCTTCGGCCTGGGCCCGCGTCTGAATGCGGCGGGCCGGC
TGGCGGATATGTCGCTCGGCATCGAGTGCCTGCTGACCGACGACGCCAATCGCGCCTGGGAAATCGCCCAGGAACTGGAC
GGCATGAACCGCGAGCGGCGCGATATCGAGGCTGGAATGCAGCAGGAAGCACTTCAGATCCTCGAACAGCCGCTAGCCGG
GCTGGACCCGACATCGCGTCACACCGTCAGCGTGTTCCATGACACGTGGCACCAGGGCGTGATCGGCATCGTCGCATCGC
GGCTCAAGGAAAAATTCCACCGCCCGACCATCACGTTCGCGCCCGGCGACGAGGAGCACATCAAGGGTTCAGGGCGATCG
ATCCCCGGTTTTCACCTGCGCGACGCGCTGGATCTGGTGTCGAAGCGCCACCCGGGACTGCTGGTCAAGTTCGGCGGACA
CGCGATGGCGGCCGGTTTGACCGTGCGCGCCAAGGACTTCGACACCTTTGTCAGCGCGTTTGAGGAAGTTGGCCGCGAAT
GGCTGACCGATGACCAATTGGCGCGTGTGATCGAGACTGATGGCGATATCGAGGATGCGTGCTTCAGCCCGGAATTCGTC
ACCCTGCTGGAAGGTCAGGTCTGGGGCCAGGGTTTCCCGGCACCAACGTTCTGCGGCGAGTTCGACGTATTGCGCCAGAC
CGTGCTCAAGGGCAAGCACCTGAAACTCCAGCTCGGTCGGGGCAGCCAGCGCTTCGACGCCATCTGGTTCAACCATGCCG
ATGCGCTTGATGGCGCCAGCGCGATGGTGGCTTACCGGCTCGACAACAACACATTCAACGGAATGACCCGGGTGCAACTG
GTGATCGAGCACGCGCAGTAA

Upstream 100 bases:

>100_bases
GGCTCGCGCGGAGACTGGCGCGCCCTGCTGTCCGACCTGGCCATGGTCTCCTGACCCGGCGGCCTGACATCACATTGACG
AATTCGAACGCGAACGCCAC

Downstream 100 bases:

>100_bases
GGGCAGCCGCTTGTTTTCTCCTCTGCCGCCTGTGGGAGAGGGAAGCGAACCGCCTAACAGGCGGCTCTTACCCCCTAGCC
CCGAGAAGGCAGATAATTCG

Product: ssDNA exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MTRIAIRSFSDEHAKALAASGLHPTLARILSARGVAQPSELATDLPELAPPASMKGIGHAAEYLADAIAANRKLLIVADY
DCDGATACAVGVRGLRMLGARVDYIVPNRFEYGYGLTPEIVELAARQHPDVIVTVDNGIASVDGVAAANARGIDVVVTDH
HLPGETLPEAAVIVNPNQPGCEFPSKNLAGVGVMFYVLLALRAELRRRGVYTQATQPPLQTLLDLVALGTVADVVKLDTN
NRILVAQGLKRMRAGRMHPGVAALFRAAGREASRANTFDLGFGLGPRLNAAGRLADMSLGIECLLTDDANRAWEIAQELD
GMNRERRDIEAGMQQEALQILEQPLAGLDPTSRHTVSVFHDTWHQGVIGIVASRLKEKFHRPTITFAPGDEEHIKGSGRS
IPGFHLRDALDLVSKRHPGLLVKFGGHAMAAGLTVRAKDFDTFVSAFEEVGREWLTDDQLARVIETDGDIEDACFSPEFV
TLLEGQVWGQGFPAPTFCGEFDVLRQTVLKGKHLKLQLGRGSQRFDAIWFNHADALDGASAMVAYRLDNNTFNGMTRVQL
VIEHAQ

Sequences:

>Translated_566_residues
MTRIAIRSFSDEHAKALAASGLHPTLARILSARGVAQPSELATDLPELAPPASMKGIGHAAEYLADAIAANRKLLIVADY
DCDGATACAVGVRGLRMLGARVDYIVPNRFEYGYGLTPEIVELAARQHPDVIVTVDNGIASVDGVAAANARGIDVVVTDH
HLPGETLPEAAVIVNPNQPGCEFPSKNLAGVGVMFYVLLALRAELRRRGVYTQATQPPLQTLLDLVALGTVADVVKLDTN
NRILVAQGLKRMRAGRMHPGVAALFRAAGREASRANTFDLGFGLGPRLNAAGRLADMSLGIECLLTDDANRAWEIAQELD
GMNRERRDIEAGMQQEALQILEQPLAGLDPTSRHTVSVFHDTWHQGVIGIVASRLKEKFHRPTITFAPGDEEHIKGSGRS
IPGFHLRDALDLVSKRHPGLLVKFGGHAMAAGLTVRAKDFDTFVSAFEEVGREWLTDDQLARVIETDGDIEDACFSPEFV
TLLEGQVWGQGFPAPTFCGEFDVLRQTVLKGKHLKLQLGRGSQRFDAIWFNHADALDGASAMVAYRLDNNTFNGMTRVQL
VIEHAQ
>Mature_565_residues
TRIAIRSFSDEHAKALAASGLHPTLARILSARGVAQPSELATDLPELAPPASMKGIGHAAEYLADAIAANRKLLIVADYD
CDGATACAVGVRGLRMLGARVDYIVPNRFEYGYGLTPEIVELAARQHPDVIVTVDNGIASVDGVAAANARGIDVVVTDHH
LPGETLPEAAVIVNPNQPGCEFPSKNLAGVGVMFYVLLALRAELRRRGVYTQATQPPLQTLLDLVALGTVADVVKLDTNN
RILVAQGLKRMRAGRMHPGVAALFRAAGREASRANTFDLGFGLGPRLNAAGRLADMSLGIECLLTDDANRAWEIAQELDG
MNRERRDIEAGMQQEALQILEQPLAGLDPTSRHTVSVFHDTWHQGVIGIVASRLKEKFHRPTITFAPGDEEHIKGSGRSI
PGFHLRDALDLVSKRHPGLLVKFGGHAMAAGLTVRAKDFDTFVSAFEEVGREWLTDDQLARVIETDGDIEDACFSPEFVT
LLEGQVWGQGFPAPTFCGEFDVLRQTVLKGKHLKLQLGRGSQRFDAIWFNHADALDGASAMVAYRLDNNTFNGMTRVQLV
IEHAQ

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=553, Percent_Identity=47.377938517179, Blast_Score=478, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 61281; Mature: 61149

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIAIRSFSDEHAKALAASGLHPTLARILSARGVAQPSELATDLPELAPPASMKGIGHA
CCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCHHHCCCCCCCCCHHH
AEYLADAIAANRKLLIVADYDCDGATACAVGVRGLRMLGARVDYIVPNRFEYGYGLTPEI
HHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHH
VELAARQHPDVIVTVDNGIASVDGVAAANARGIDVVVTDHHLPGETLPEAAVIVNPNQPG
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCC
CEFPSKNLAGVGVMFYVLLALRAELRRRGVYTQATQPPLQTLLDLVALGTVADVVKLDTN
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCC
NRILVAQGLKRMRAGRMHPGVAALFRAAGREASRANTFDLGFGLGPRLNAAGRLADMSLG
CEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCEECCCC
IECLLTDDANRAWEIAQELDGMNRERRDIEAGMQQEALQILEQPLAGLDPTSRHTVSVFH
EEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEH
DTWHQGVIGIVASRLKEKFHRPTITFAPGDEEHIKGSGRSIPGFHLRDALDLVSKRHPGL
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCE
LVKFGGHAMAAGLTVRAKDFDTFVSAFEEVGREWLTDDQLARVIETDGDIEDACFSPEFV
EEEECCCEEECCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCHH
TLLEGQVWGQGFPAPTFCGEFDVLRQTVLKGKHLKLQLGRGSQRFDAIWFNHADALDGAS
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCCCC
AMVAYRLDNNTFNGMTRVQLVIEHAQ
EEEEEEECCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
TRIAIRSFSDEHAKALAASGLHPTLARILSARGVAQPSELATDLPELAPPASMKGIGHA
CEEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCHHHCCCCCCCCCHHH
AEYLADAIAANRKLLIVADYDCDGATACAVGVRGLRMLGARVDYIVPNRFEYGYGLTPEI
HHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHH
VELAARQHPDVIVTVDNGIASVDGVAAANARGIDVVVTDHHLPGETLPEAAVIVNPNQPG
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCC
CEFPSKNLAGVGVMFYVLLALRAELRRRGVYTQATQPPLQTLLDLVALGTVADVVKLDTN
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCC
NRILVAQGLKRMRAGRMHPGVAALFRAAGREASRANTFDLGFGLGPRLNAAGRLADMSLG
CEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCEECCCC
IECLLTDDANRAWEIAQELDGMNRERRDIEAGMQQEALQILEQPLAGLDPTSRHTVSVFH
EEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEH
DTWHQGVIGIVASRLKEKFHRPTITFAPGDEEHIKGSGRSIPGFHLRDALDLVSKRHPGL
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCE
LVKFGGHAMAAGLTVRAKDFDTFVSAFEEVGREWLTDDQLARVIETDGDIEDACFSPEFV
EEEECCCEEECCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCHH
TLLEGQVWGQGFPAPTFCGEFDVLRQTVLKGKHLKLQLGRGSQRFDAIWFNHADALDGAS
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCCCC
AMVAYRLDNNTFNGMTRVQLVIEHAQ
EEEEEEECCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8168497 [H]