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The map label for this gene is prfB [H]

Identifier: 94309981

GI number: 94309981

Start: 1125147

End: 1126220

Strand: Reverse

Name: prfB [H]

Synonym: Rmet_1036

Alternate gene names: 94309981

Gene position: 1126220-1125147 (Counterclockwise)

Preceding gene: 94309983

Following gene: 94309980

Centisome position: 28.67

GC content: 62.29

Gene sequence:

>1074_bases
GTGCAATCTCCGATCTCCGTTCCCGGACGGAAGATCTACGGGGGTATCTTTGACTACGATGTCAAAGTAAACCGGCTGGA
TGAAGTCAACGGGTTGCTCGAAGACCCCGACGTCTGGAACAACCCCAAGCGAGCCCAGGACCTGGGCAAGGAAAAGAAGG
CGCTGGAAGGCGTCGTGCAGGTACTCGGCAAGCTGACCGACGACCTCAACGGCACGGACGAGCTGTTCGAGCTTGCCCGC
GAGGAAGGCGACGACGAGACACTCGAATCCATCGAGGCCGACGTCTCTGGCTTCGAGGCCCTGGTGGCGGACATGGAGTT
CCGCCGCATGTTCTCGGGCGAGATGGACCAGGCCAATGCGTTCATCGACATCCAGGCGGGCGCCGGCGGCACCGAGGCCT
GCGACTGGGCGTCGATGCTGCTGCGCCAGTACCTGAAGTACTGCGAGCGCAAGGGTTTCAAGGCCGAAGTGCTGGAAGAA
TCCGAAGGCGACGTAGCCGGCATCAAGAGCGCGACGATCAAGGTCGAGGGCGAATACGCGTTCGGCTACCTGCGCACGGA
AACCGGTGTGCATCGCCTGGTGCGGAAGTCGCCGTTCGATTCGTCGGGCGGGCGCCATACGTCGTTCTCGTCGGTCTTCG
TCTACCCCGAGGTGGATGATTCGTTCGAGGTGGAGGTCAACCCGGCCGATCTGCGCGTGGATACCTACCGCGCGTCGGGT
GCCGGTGGTCAGCACATCAACAAGACCGACTCCGCCGTGCGGATTACGCACATGCCTACCGGCATCGTCGTGCAGTGCCA
GAACGACCGTTCGCAGCACCGCAACCGCGCCGAGGCCATGTCCATGCTGAAGTCGCGCCTGTACGAGCATGAAATGCGCA
AGCGTCAGGCGGAAGCCGACAAGCTCGAAGCCGGCAAGACGGACGTGGGCTGGGGCCACCAGATCCGCTCGTACGTGCTG
GACCAGAGCCGTATCAAGGACCTGCGCACCAACGTGGAAATGTCCAACACCCAGAAGGTGCTGGATGGTGACCTCGACCC
GTTCATCGAGGCCAGCCTGAAACAGGGGTTGTAA

Upstream 100 bases:

>100_bases
CGCCCCACCCCGGATTTGCCCCGTCAATCCGCTATAATTCCCGGTTTTGAAGCGCAAGAACACTACAACATGGAAGCAGA
ACGCCTGAACGCTATCTCCG

Downstream 100 bases:

>100_bases
TCCAGACGCCTGATACTCAGGTCCTGGCAATTTCTGCCGAAGATTGCAGATAGACGTCGCCGCGCAATGTCCCCGGACAA
ACTCGGGGCCTGCGCGGTGA

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MQSPISVPGRKIYGGIFDYDVKVNRLDEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVQVLGKLTDDLNGTDELFELAR
EEGDDETLESIEADVSGFEALVADMEFRRMFSGEMDQANAFIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEE
SEGDVAGIKSATIKVEGEYAFGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASG
AGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEADKLEAGKTDVGWGHQIRSYVL
DQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL

Sequences:

>Translated_357_residues
MQSPISVPGRKIYGGIFDYDVKVNRLDEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVQVLGKLTDDLNGTDELFELAR
EEGDDETLESIEADVSGFEALVADMEFRRMFSGEMDQANAFIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEE
SEGDVAGIKSATIKVEGEYAFGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASG
AGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEADKLEAGKTDVGWGHQIRSYVL
DQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL
>Mature_357_residues
MQSPISVPGRKIYGGIFDYDVKVNRLDEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVQVLGKLTDDLNGTDELFELAR
EEGDDETLESIEADVSGFEALVADMEFRRMFSGEMDQANAFIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEE
SEGDVAGIKSATIKVEGEYAFGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASG
AGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEADKLEAGKTDVGWGHQIRSYVL
DQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=250, Percent_Identity=38.4, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=32.8125, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=39.5973154362416, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=65.0145772594752, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1787462, Length=244, Percent_Identity=43.8524590163934, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=302, Percent_Identity=30.1324503311258, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=242, Percent_Identity=36.3636363636364, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19921226, Length=209, Percent_Identity=43.0622009569378, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39889; Mature: 39889

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSPISVPGRKIYGGIFDYDVKVNRLDEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVQ
CCCCCCCCCHHHCCCEEECCEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLGKLTDDLNGTDELFELAREEGDDETLESIEADVSGFEALVADMEFRRMFSGEMDQANA
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
FIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEESEGDVAGIKSATIKVEGEYA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEECCCEEEEEEECCEE
FGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASG
EEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCC
AGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEAD
CCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLEAGKTDVGWGHQIRSYVLDQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQSPISVPGRKIYGGIFDYDVKVNRLDEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVQ
CCCCCCCCCHHHCCCEEECCEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLGKLTDDLNGTDELFELAREEGDDETLESIEADVSGFEALVADMEFRRMFSGEMDQANA
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
FIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEESEGDVAGIKSATIKVEGEYA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEECCCEEEEEEECCEE
FGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASG
EEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCC
AGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEAD
CCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLEAGKTDVGWGHQIRSYVLDQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA