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The map label for this gene is phaZ1

Identifier: 94309962

GI number: 94309962

Start: 1106373

End: 1107617

Strand: Reverse

Name: phaZ1

Synonym: Rmet_1017

Alternate gene names: NA

Gene position: 1107617-1106373 (Counterclockwise)

Preceding gene: 94309963

Following gene: 94309960

Centisome position: 28.2

GC content: 61.29

Gene sequence:

>1245_bases
ATGCTTTATCAACTGCATGAATTCCAGCGCACGATGCTGAACCCGCTTACCGCGTGGGCCCAAGCGACTGCAAAGACATT
TACGAACCCCCTCAGCCCCCTTTCACTGGTCCCCGGCGCCCCTCGGCTGGCAGCAGGCTACGAACTGCTGTACCGATTGG
GAAAAGCCTACGAAAAGCCGGAATTCAACATCAAATCCGTCCGTTCGAATGGCCGCGACATTCCGATCGTCGAACAAACC
ATCCTGGACAAGCCGTTCTGCAAGCTGGTGCGCTTCAAGCGCTATGCGGACGATCCGGACACCATCAAGCTGCTCAAGGA
CGAGCCGGTCGTGCTGGTCTGCGCGCCGCTGTCCGGTCACCACTCCACGCTGCTGCGCGATACCGTGCGTACTTTGCTGC
AGGACCACAAAGTCTATGTGACCGACTGGGTGGACGCCCGCATGGTGCCGGTGGAGCATGGTGAGTTCCATCTGTCCGAC
TACATCCGTTACATCCAGGAGTTCATCCGTCACATTGGCGCGGAGAACCTGAATGTGATCTCCGTGTGCCAGCCGACTGT
GCCGGTGCTCGCCGCGATCTCGCTGATGGCGTCGGCAGGTGAGAAGACCCCGCGCACCATGACGATGATGGGTGGCCCGA
TCGATGCGCGAAAGAGCGCAACGGCGGTCAACTCGCTCGCCACCAACAAGTCCTACGAGTGGTTCGAGAACAACGTCATC
TACACGGTGCCGGCCAACTACCCGGGCCACGGCCGCCGCGTCTATCCAGGCTTCCTGCAGCACGCTGGCTTCGTGGCGAT
GAACCCGGACCGCCATGTGTCATCGCACTATGACTACTACCTGAGCCTGGTCCAGGGCGGGTCCGACGACGCCGAGGCGC
ACGTCAAGTTCTATGACGAGTACAACGCCGTGCTCGACATGGCGGCCGAGTACTACCTTGAGACGATTCGCACGGTGTTC
CAGGAATTCCGCCTGGCCAATGGCACGTGGGTCGTCGACGGCGAGCCCGTGCGTCCGCAGGACATCAAGAGCACTGCCCT
GCTGACGATCGAAGGCGAGCTCGACGATATTTCGGGCGCCGGCCAGACCGCTGCCGCGCACGACTTGTGCACCGGTATCG
CGAAAACCCGCAAGCAACACCTGACCGCCGAGAAATGCGGCCACTACGGGATTTTCTCGGGCCGCCGCTGGCGCGATAGC
GTCTATCCGGAACTGCGCGATTTCATCCGCAAGTACCGCGCCTGA

Upstream 100 bases:

>100_bases
CAACGATGAGGTTTATGCTGCAAAGCACGATCGTTCTATTCCGTGCATAATCGGTTGCACGACATCCGTTGGCGACAAGA
AAGGCTAGACAGGTACCCCC

Downstream 100 bases:

>100_bases
CCGTCGCGCGTCGCATCAAAGTCAAACGCCCGCGAAGACTCGCGGGCGTTTTTTTATCTGCCGATGCCGGCGACAGCTTC
AGCGCTTCAGGTACGACAGC

Product: intracellular poly(3-hydroxybutyrate) depolymerase

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; PHB De-Polymerase Domain-Containing Protein; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MLYQLHEFQRTMLNPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKAYEKPEFNIKSVRSNGRDIPIVEQT
ILDKPFCKLVRFKRYADDPDTIKLLKDEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYVTDWVDARMVPVEHGEFHLSD
YIRYIQEFIRHIGAENLNVISVCQPTVPVLAAISLMASAGEKTPRTMTMMGGPIDARKSATAVNSLATNKSYEWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVQGGSDDAEAHVKFYDEYNAVLDMAAEYYLETIRTVF
QEFRLANGTWVVDGEPVRPQDIKSTALLTIEGELDDISGAGQTAAAHDLCTGIAKTRKQHLTAEKCGHYGIFSGRRWRDS
VYPELRDFIRKYRA

Sequences:

>Translated_414_residues
MLYQLHEFQRTMLNPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKAYEKPEFNIKSVRSNGRDIPIVEQT
ILDKPFCKLVRFKRYADDPDTIKLLKDEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYVTDWVDARMVPVEHGEFHLSD
YIRYIQEFIRHIGAENLNVISVCQPTVPVLAAISLMASAGEKTPRTMTMMGGPIDARKSATAVNSLATNKSYEWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVQGGSDDAEAHVKFYDEYNAVLDMAAEYYLETIRTVF
QEFRLANGTWVVDGEPVRPQDIKSTALLTIEGELDDISGAGQTAAAHDLCTGIAKTRKQHLTAEKCGHYGIFSGRRWRDS
VYPELRDFIRKYRA
>Mature_414_residues
MLYQLHEFQRTMLNPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKAYEKPEFNIKSVRSNGRDIPIVEQT
ILDKPFCKLVRFKRYADDPDTIKLLKDEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYVTDWVDARMVPVEHGEFHLSD
YIRYIQEFIRHIGAENLNVISVCQPTVPVLAAISLMASAGEKTPRTMTMMGGPIDARKSATAVNSLATNKSYEWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVQGGSDDAEAHVKFYDEYNAVLDMAAEYYLETIRTVF
QEFRLANGTWVVDGEPVRPQDIKSTALLTIEGELDDISGAGQTAAAHDLCTGIAKTRKQHLTAEKCGHYGIFSGRRWRDS
VYPELRDFIRKYRA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46748; Mature: 46748

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYQLHEFQRTMLNPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKAYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCC
EFNIKSVRSNGRDIPIVEQTILDKPFCKLVRFKRYADDPDTIKLLKDEPVVLVCAPLSGH
CCCHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCC
HSTLLRDTVRTLLQDHKVYVTDWVDARMVPVEHGEFHLSDYIRYIQEFIRHIGAENLNVI
HHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCEE
SVCQPTVPVLAAISLMASAGEKTPRTMTMMGGPIDARKSATAVNSLATNKSYEWFENNVI
EECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHCCEE
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVQGGSDDAEAHVKFYDE
EEECCCCCCCCCEECCHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
YNAVLDMAAEYYLETIRTVFQEFRLANGTWVVDGEPVRPQDIKSTALLTIEGELDDISGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCC
GQTAAAHDLCTGIAKTRKQHLTAEKCGHYGIFSGRRWRDSVYPELRDFIRKYRA
CCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MLYQLHEFQRTMLNPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKAYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCC
EFNIKSVRSNGRDIPIVEQTILDKPFCKLVRFKRYADDPDTIKLLKDEPVVLVCAPLSGH
CCCHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCC
HSTLLRDTVRTLLQDHKVYVTDWVDARMVPVEHGEFHLSDYIRYIQEFIRHIGAENLNVI
HHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCEE
SVCQPTVPVLAAISLMASAGEKTPRTMTMMGGPIDARKSATAVNSLATNKSYEWFENNVI
EECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHCCEE
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVQGGSDDAEAHVKFYDE
EEECCCCCCCCCEECCHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
YNAVLDMAAEYYLETIRTVFQEFRLANGTWVVDGEPVRPQDIKSTALLTIEGELDDISGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCC
GQTAAAHDLCTGIAKTRKQHLTAEKCGHYGIFSGRRWRDSVYPELRDFIRKYRA
CCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA