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The map label for this gene is nuoM [H]

Identifier: 94309884

GI number: 94309884

Start: 1024597

End: 1026063

Strand: Direct

Name: nuoM [H]

Synonym: Rmet_0939

Alternate gene names: 94309884

Gene position: 1024597-1026063 (Clockwise)

Preceding gene: 94309883

Following gene: 94309885

Centisome position: 26.08

GC content: 59.71

Gene sequence:

>1467_bases
ATGGTTCTGTCTTTCGCAATCTGGCTGCCTATCTTCTTCGGGCTGGTGGTATTGATGTTCGGCTCCGACCGAAGCAAATG
CTTCGTGCGCTGGATGGCGCTGTTCGGTGCTATCGTCAGCTTTGCCGTCACGCTGCCGCTGATCTTCCACTTCGACACCG
CTTCCGCTGCGATGCAGTTCGTCGAGCAGGCGACGTGGATCGAACGCTTCAACATCCAGTACCACCTGGGTGTGGATGGC
ATCTCGATGTGGTTCGTGGTGCTGACCGCATTCATCACCGTGATCGTCGTGATCTCGGCCTGGGAAGTGATCACCGAACG
CGTGGCCGAGTACATGGCCTCGTTCCTGATCCTGTCAGGCTTGATGGTCGGTGTCTTCTCGTCGCTGGACGGCCTGCTGT
TCTACGTGTTCTTCGAAGCCACGCTGATCCCGATGTACATCATCATCGGTGTCTGGGGCGGCCCGAACCGCGTGTATGCG
GCGTTCAAGTTCTTCCTCTACACGCTGCTCGGCTCGCTGCTGACCCTGGTCGCGCTGCTGTACCTGTACAACAAGACGGG
CTCGTTCGACATCCTCACCTGGCATCAGGCCAAGTTGACGATGAACGAGCAGATCGCGATCTTCCTCGCGTTCTTCATGG
CCTTCGCGGTGAAGGTGCCGATGTGGCCGGTGCACACCTGGCTGCCGGACGCCCACGTGGAAGCGCCGACCGGCGGTTCC
GTGGTGCTGGCGGCGATCATGCTGAAGCTGGGCGCCTACGGTTTCCTGCGGTTCTCGCTGCCGATCGCCCCGGACGCCAG
CCACTACCTGGCCCCGTTCATCATCACGATCTCGCTGATCGCCGTGATCTACATCGGCCTCGTGGCACTGGTCCAGGCCG
ACATGAAGAAGCTGGTGGCGTACTCGTCGATCGCTCACATGGGTTTCGTGACGCTCGGTTTCTTCATCTTCAATGAAATC
GGCATCGAGGGCGGCATCATCCAGATGATCTCGCATGGTTTCGTGTCGGGCGCGATGTTCCTGTGTATCGGTGTGCTCTA
TGACCGCGTGCACTCGCGCCAGATCGCCGACTACGGCGGTGTGGTGAACGTGATGCCGAAGTTCGCGGCGCTGTCGGTGT
TCTTCGCGATGGCCAACTGTGGCCTGCCGGCTACCTCGGGTTTCGTGGGCGAGTTCATGGTGATCCTGGGTGCTGTCAAG
TTCAACTTCTGGATTGGCCTGCTGGCCGCCACGGCCCTGATCTTCGGCGCGGCTTACTCGCTGTGGATGGTCAAGCGCGT
GATCTTCGGCGATATCGTGCACGAGCACGTGCGTGAACTGGTGGACCTGAACAAGCGTGAATTCTTCATGCTCGGTCTGC
TGGCGATCATGACGCTGTACATGGGTCTGTACCCGAAACCCTTCACCGACGTGATGCACGCATCGGTGGTGAACCTGCTG
TCCCACGTGGCACAGTCCAAGCTGTAA

Upstream 100 bases:

>100_bases
GCTTTCGCGATGATCATCGGCATGTTGGTGCTCCTGACGCTGACGATCACTGGCGTGATAGGCACCAAGTGATAGGCACC
AAAGCAAGGAAAACATAGAA

Downstream 100 bases:

>100_bases
GCGGGGGAGAGAAATCAACATGCAATCGTCCTCTAGCCTGGCCCCGGTGGCGCCAATCATTTTCCTGGCGATCGCCATTG
CGGCGATCAACTGGATCGAC

Product: NADH:ubiquinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MVLSFAIWLPIFFGLVVLMFGSDRSKCFVRWMALFGAIVSFAVTLPLIFHFDTASAAMQFVEQATWIERFNIQYHLGVDG
ISMWFVVLTAFITVIVVISAWEVITERVAEYMASFLILSGLMVGVFSSLDGLLFYVFFEATLIPMYIIIGVWGGPNRVYA
AFKFFLYTLLGSLLTLVALLYLYNKTGSFDILTWHQAKLTMNEQIAIFLAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHYLAPFIITISLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GIEGGIIQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGGVVNVMPKFAALSVFFAMANCGLPATSGFVGEFMVILGAVK
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDIVHEHVRELVDLNKREFFMLGLLAIMTLYMGLYPKPFTDVMHASVVNLL
SHVAQSKL

Sequences:

>Translated_488_residues
MVLSFAIWLPIFFGLVVLMFGSDRSKCFVRWMALFGAIVSFAVTLPLIFHFDTASAAMQFVEQATWIERFNIQYHLGVDG
ISMWFVVLTAFITVIVVISAWEVITERVAEYMASFLILSGLMVGVFSSLDGLLFYVFFEATLIPMYIIIGVWGGPNRVYA
AFKFFLYTLLGSLLTLVALLYLYNKTGSFDILTWHQAKLTMNEQIAIFLAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHYLAPFIITISLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GIEGGIIQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGGVVNVMPKFAALSVFFAMANCGLPATSGFVGEFMVILGAVK
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDIVHEHVRELVDLNKREFFMLGLLAIMTLYMGLYPKPFTDVMHASVVNLL
SHVAQSKL
>Mature_488_residues
MVLSFAIWLPIFFGLVVLMFGSDRSKCFVRWMALFGAIVSFAVTLPLIFHFDTASAAMQFVEQATWIERFNIQYHLGVDG
ISMWFVVLTAFITVIVVISAWEVITERVAEYMASFLILSGLMVGVFSSLDGLLFYVFFEATLIPMYIIIGVWGGPNRVYA
AFKFFLYTLLGSLLTLVALLYLYNKTGSFDILTWHQAKLTMNEQIAIFLAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHYLAPFIITISLIAVIYIGLVALVQADMKKLVAYSSIAHMGFVTLGFFIFNEI
GIEGGIIQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGGVVNVMPKFAALSVFFAMANCGLPATSGFVGEFMVILGAVK
FNFWIGLLAATALIFGAAYSLWMVKRVIFGDIVHEHVRELVDLNKREFFMLGLLAIMTLYMGLYPKPFTDVMHASVVNLL
SHVAQSKL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=346, Percent_Identity=36.4161849710983, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI251831117, Length=398, Percent_Identity=24.6231155778894, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1788613, Length=498, Percent_Identity=37.7510040160643, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1788827, Length=272, Percent_Identity=25.3676470588235, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788829, Length=470, Percent_Identity=27.4468085106383, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788831, Length=292, Percent_Identity=25.6849315068493, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693160, Length=454, Percent_Identity=23.1277533039648, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788614, Length=414, Percent_Identity=24.1545893719807, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI2367154, Length=244, Percent_Identity=25.4098360655738, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54177; Mature: 54177

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSFAIWLPIFFGLVVLMFGSDRSKCFVRWMALFGAIVSFAVTLPLIFHFDTASAAMQF
CEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHH
VEQATWIERFNIQYHLGVDGISMWFVVLTAFITVIVVISAWEVITERVAEYMASFLILSG
HHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMVGVFSSLDGLLFYVFFEATLIPMYIIIGVWGGPNRVYAAFKFFLYTLLGSLLTLVALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLYNKTGSFDILTWHQAKLTMNEQIAIFLAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
HHHCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHH
VVLAAIMLKLGAYGFLRFSLPIAPDASHYLAPFIITISLIAVIYIGLVALVQADMKKLVA
HHHHHHHHHHCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSSIAHMGFVTLGFFIFNEIGIEGGIIQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VVNVMPKFAALSVFFAMANCGLPATSGFVGEFMVILGAVKFNFWIGLLAATALIFGAAYS
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWMVKRVIFGDIVHEHVRELVDLNKREFFMLGLLAIMTLYMGLYPKPFTDVMHASVVNLL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SHVAQSKL
HHHHHCCC
>Mature Secondary Structure
MVLSFAIWLPIFFGLVVLMFGSDRSKCFVRWMALFGAIVSFAVTLPLIFHFDTASAAMQF
CEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHH
VEQATWIERFNIQYHLGVDGISMWFVVLTAFITVIVVISAWEVITERVAEYMASFLILSG
HHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMVGVFSSLDGLLFYVFFEATLIPMYIIIGVWGGPNRVYAAFKFFLYTLLGSLLTLVALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLYNKTGSFDILTWHQAKLTMNEQIAIFLAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
HHHCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHH
VVLAAIMLKLGAYGFLRFSLPIAPDASHYLAPFIITISLIAVIYIGLVALVQADMKKLVA
HHHHHHHHHHCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSSIAHMGFVTLGFFIFNEIGIEGGIIQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VVNVMPKFAALSVFFAMANCGLPATSGFVGEFMVILGAVKFNFWIGLLAATALIFGAAYS
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWMVKRVIFGDIVHEHVRELVDLNKREFFMLGLLAIMTLYMGLYPKPFTDVMHASVVNLL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SHVAQSKL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]