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The map label for this gene is nuoF [H]

Identifier: 94309877

GI number: 94309877

Start: 1015973

End: 1017268

Strand: Direct

Name: nuoF [H]

Synonym: Rmet_0932

Alternate gene names: 94309877

Gene position: 1015973-1017268 (Clockwise)

Preceding gene: 94309876

Following gene: 94309878

Centisome position: 25.86

GC content: 61.73

Gene sequence:

>1296_bases
ATGACCTCTCTGCATGACCGTCATATCAAGCCGCTGATTCTGGCTGGCCTGGACGGCAAGAACTGGCACCTCGAAGACTA
CGTGAAGCGTGGCGGCTACAAGCAGCTCCGCCGCATTCTCGAGGAAAAGGTAACGCCCGAGCAGGTGATCGCCGACGTCA
AGGCGTCGGGTCTGCGCGGCCGTGGCGGTGCGGGCTTCCCCACCGGCCTGAAGTGGAGCTTCATGCCGCGTGCGTTCCCG
GGTCAGAAGTACCTCGTCTGCAATACCGATGAAGGCGAACCGGGCACGTTCAAGGATCGCGACATCATTCGCTACAACCC
GCACGCGCTGATCGAAGGCATGGCCATCGGCGCGTACGCGATGGGCATCACCGTGGGTTACAACTACATCCACGGCGAAA
TCTGGAACGAATACAAGATCTTCGAGGAAGCCCTCGAAGAAGCGCGCCGCGCCGGCTTCCTCGGCGACAACATCCTGGGT
TCGGGCTTCAACTTCCAGTTGCACGCCCACCATGGCTACGGCGCATACATCTGCGGCGAGGAAACCGCGCTGCTGGAGTC
GCTCGAAGGCAAGAAGGGCCAACCGCGCTTCAAGCCGCCTTTCCCGGCCAGCTTCGGCCTGTACGGCAAGCCGACCACCA
TCAACAATACGGAAACGTTCGCCGCGGTGCCGTTCCTGCTGGAAGTCGGCCCCGAGAACTACCTGAAGATGGGCAAGCCG
AACAACGGCGGCAGCAAGATCTTCTCGGTGTCGGGCGATGTGGAGCGTCCGGGCAACTACGAGATCCCGCTGGGCACGCC
GTTCGCAACGCTGCTGGAACTCGCGGGCGGCATGCGCGGTGGCAAGAAGATCAAGGCGGTCATTCCGGGCGGTTCCTCCG
CCCCGGTGGTGCCGGGCGACCTGATGATGGCATCGGACATGGATTACGACTCGATCGCCAAGGCCGGCTCGATGCTGGGC
TCGGGCGCCGTGATCGTGATGGACGAAACACGCTGCATGGTGCGTTCGCTCCTGCGCCTGTCGTATTTCTACTTTGAAGA
ATCGTGCGGCCAGTGCACGCCGTGCCGTGAAGGCACCGGCTGGCTCTATCGCATGGTGAATCGCATTGAACACGGAGAAG
GCCGCCAGGAAGATCTGGACCTGCTCAACAACGTCGCGGAAAACATCATGGGTCGCACTATCTGCGCACTCGGCGATGCA
GCGGCGATGCCGGTACGCGGCATGCTCAAGCACTACTGGAAGGAGTTCGAGTATCACGTCGAACACAAGCAGTGCATGGT
CCCGGCCTATTCCTGA

Upstream 100 bases:

>100_bases
GTCAACAACACCCGCATGTGCAGCTTCATGAGCGAGCAGAAGATCGACGCACTGGTCGAAGAGCTCAAGTCCGAAGCCGC
CGCCAAGGGGGACAAGTAAC

Downstream 100 bases:

>100_bases
AGCGTGGCAGAACATGGTTGAACTCGAGATCGACGGCAAGAAGGTAGAGGTTGCGGAAGGCAGCCTCGTGATGGAAGCGG
CCCAGAAGCTGGGCACGTAC

Product: NADH:ubiquinone oxidoreductase, chain F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTSLHDRHIKPLILAGLDGKNWHLEDYVKRGGYKQLRRILEEKVTPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRAFP
GQKYLVCNTDEGEPGTFKDRDIIRYNPHALIEGMAIGAYAMGITVGYNYIHGEIWNEYKIFEEALEEARRAGFLGDNILG
SGFNFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLEVGPENYLKMGKP
NNGGSKIFSVSGDVERPGNYEIPLGTPFATLLELAGGMRGGKKIKAVIPGGSSAPVVPGDLMMASDMDYDSIAKAGSMLG
SGAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTGWLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDA
AAMPVRGMLKHYWKEFEYHVEHKQCMVPAYS

Sequences:

>Translated_431_residues
MTSLHDRHIKPLILAGLDGKNWHLEDYVKRGGYKQLRRILEEKVTPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRAFP
GQKYLVCNTDEGEPGTFKDRDIIRYNPHALIEGMAIGAYAMGITVGYNYIHGEIWNEYKIFEEALEEARRAGFLGDNILG
SGFNFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLEVGPENYLKMGKP
NNGGSKIFSVSGDVERPGNYEIPLGTPFATLLELAGGMRGGKKIKAVIPGGSSAPVVPGDLMMASDMDYDSIAKAGSMLG
SGAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTGWLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDA
AAMPVRGMLKHYWKEFEYHVEHKQCMVPAYS
>Mature_430_residues
TSLHDRHIKPLILAGLDGKNWHLEDYVKRGGYKQLRRILEEKVTPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRAFPG
QKYLVCNTDEGEPGTFKDRDIIRYNPHALIEGMAIGAYAMGITVGYNYIHGEIWNEYKIFEEALEEARRAGFLGDNILGS
GFNFQLHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLEVGPENYLKMGKPN
NGGSKIFSVSGDVERPGNYEIPLGTPFATLLELAGGMRGGKKIKAVIPGGSSAPVVPGDLMMASDMDYDSIAKAGSMLGS
GAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTGWLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDAA
AMPVRGMLKHYWKEFEYHVEHKQCMVPAYS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=414, Percent_Identity=46.6183574879227, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI260656005, Length=414, Percent_Identity=46.6183574879227, Blast_Score=384, Evalue=1e-106,
Organism=Escherichia coli, GI1788620, Length=402, Percent_Identity=42.7860696517413, Blast_Score=328, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17531727, Length=431, Percent_Identity=46.6357308584687, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI19920794, Length=413, Percent_Identity=45.5205811138014, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45550452, Length=415, Percent_Identity=44.0963855421687, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24653819, Length=402, Percent_Identity=36.318407960199, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24653817, Length=402, Percent_Identity=32.5870646766169, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47587; Mature: 47456

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLHDRHIKPLILAGLDGKNWHLEDYVKRGGYKQLRRILEEKVTPEQVIADVKASGLRG
CCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRAFPGQKYLVCNTDEGEPGTFKDRDIIRYNPHALIEGMAIGAYA
CCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHEE
MGITVGYNYIHGEIWNEYKIFEEALEEARRAGFLGDNILGSGFNFQLHAHHGYGAYICGE
EEHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLEVGPENYLKMGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCHHHHCCCCC
NNGGSKIFSVSGDVERPGNYEIPLGTPFATLLELAGGMRGGKKIKAVIPGGSSAPVVPGD
CCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
LMMASDMDYDSIAKAGSMLGSGAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTG
EEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
WLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDAAAMPVRGMLKHYWKEFEYHV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHC
EHKQCMVPAYS
CHHHEECCCCC
>Mature Secondary Structure 
TSLHDRHIKPLILAGLDGKNWHLEDYVKRGGYKQLRRILEEKVTPEQVIADVKASGLRG
CCCCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRAFPGQKYLVCNTDEGEPGTFKDRDIIRYNPHALIEGMAIGAYA
CCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHEE
MGITVGYNYIHGEIWNEYKIFEEALEEARRAGFLGDNILGSGFNFQLHAHHGYGAYICGE
EEHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTETFAAVPFLLEVGPENYLKMGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCHHHHCCCCC
NNGGSKIFSVSGDVERPGNYEIPLGTPFATLLELAGGMRGGKKIKAVIPGGSSAPVVPGD
CCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
LMMASDMDYDSIAKAGSMLGSGAVIVMDETRCMVRSLLRLSYFYFEESCGQCTPCREGTG
EEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
WLYRMVNRIEHGEGRQEDLDLLNNVAENIMGRTICALGDAAAMPVRGMLKHYWKEFEYHV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHC
EHKQCMVPAYS
CHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]