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The map label for this gene is nuoE [H]

Identifier: 94309876

GI number: 94309876

Start: 1015465

End: 1015971

Strand: Direct

Name: nuoE [H]

Synonym: Rmet_0931

Alternate gene names: 94309876

Gene position: 1015465-1015971 (Clockwise)

Preceding gene: 94309875

Following gene: 94309877

Centisome position: 25.85

GC content: 61.54

Gene sequence:

>507_bases
ATGACCATGCTATCAGCAGAAGCTCTCAAGGAAATCGATCGCGCGATCGCGAAGTATCCGGCCGACCAGAAGCAGTCGGC
CGTGATGGCGGCACTCGCCGTGGCACAGGGCGAGGTGGGCTGGGTTTCCCCCGAAGTCATGCAGTTCGTCGCCAGCTACC
TCGAAATGCCGCCTGTGTGGGTGGAAGAGGTGGCCACGTTCTACAACATGTATGACACCAAGCCGGTGGGCAAGCACAAG
CTCGCCGTCTGTACCAACCTGCCGTGCGCGCTGTCGGGCGGCGAGCGGGCTGGCGAATACCTGAAGCGCAAGCTCGGGAT
CGACTATAACGAGACCACCGCCGACGGCTGTTTCACCCTGAAAGAGGGCGAGTGCATGGGCGCGTGCGGCGACGCACCGG
TGATGATCGTCAACAACACCCGCATGTGCAGCTTCATGAGCGAGCAGAAGATCGACGCACTGGTCGAAGAGCTCAAGTCC
GAAGCCGCCGCCAAGGGGGACAAGTAA

Upstream 100 bases:

>100_bases
ACGGATCGGCAGGGACGCGAAGGGGCGTCCGGGCCGGCAGAACATGGCAGCGACGGCTGCAAACTAGCGGAGCCCCGCGC
GGCTTCGCCGTATTACTGCA

Downstream 100 bases:

>100_bases
CATGACCTCTCTGCATGACCGTCATATCAAGCCGCTGATTCTGGCTGGCCTGGACGGCAAGAACTGGCACCTCGAAGACT
ACGTGAAGCGTGGCGGCTAC

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLEMPPVWVEEVATFYNMYDTKPVGKHK
LAVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTLKEGECMGACGDAPVMIVNNTRMCSFMSEQKIDALVEELKS
EAAAKGDK

Sequences:

>Translated_168_residues
MTMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLEMPPVWVEEVATFYNMYDTKPVGKHK
LAVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTLKEGECMGACGDAPVMIVNNTRMCSFMSEQKIDALVEELKS
EAAAKGDK
>Mature_167_residues
TMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLEMPPVWVEEVATFYNMYDTKPVGKHKL
AVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTLKEGECMGACGDAPVMIVNNTRMCSFMSEQKIDALVEELKSE
AAAKGDK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=162, Percent_Identity=40.1234567901235, Blast_Score=128, Evalue=2e-30,
Organism=Escherichia coli, GI1788621, Length=156, Percent_Identity=31.4102564102564, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=157, Percent_Identity=38.2165605095541, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18859877, Length=158, Percent_Identity=37.3417721518987, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355353, Length=158, Percent_Identity=35.4430379746835, Blast_Score=109, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18260; Mature: 18129

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
9.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLEMPPVW
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHH
VEEVATFYNMYDTKPVGKHKLAVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTL
HHHHHHHHHHHCCCCCCCCCEEHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEE
KEGECMGACGDAPVMIVNNTRMCSFMSEQKIDALVEELKSEAAAKGDK
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLEMPPVW
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHH
VEEVATFYNMYDTKPVGKHKLAVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTL
HHHHHHHHHHHCCCCCCCCCEEHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEE
KEGECMGACGDAPVMIVNNTRMCSFMSEQKIDALVEELKSEAAAKGDK
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]