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The map label for this gene is guaD [H]

Identifier: 94309834

GI number: 94309834

Start: 973599

End: 974939

Strand: Reverse

Name: guaD [H]

Synonym: Rmet_0889

Alternate gene names: 94309834

Gene position: 974939-973599 (Counterclockwise)

Preceding gene: 94309835

Following gene: 94309833

Centisome position: 24.82

GC content: 65.62

Gene sequence:

>1341_bases
ATGAAGATGACGACGACTTCCCCTGCATCTGGCGCCGACAACGCCGTGACCCGCGCAATCCGCGGCCGTATCCTGCACTT
CCTGCGTGACCCGCAGTTTCACGACGACGCCTATCAGTATTGGGAAGACGGCCTCATGATCGTGAAGGCCGGCCGCATCG
CCGCAGTCGGCAATTACGCGGACCTGTCACGCGATATCCCGCAAGGCGCCGAAGTCGTCGACCATCGCGGCAAGCTGATC
GTGCCCGGGTTCATCGACACGCACATCCACTACCCGCAGACCGACATCATCGCGTCGCCGTCGCCGGGCCTGCTGCACTG
GCTGGACACCTACACGTTCCCCGAGGAGCGCCGCTTCAGCGACCCCGCCTATGCACGGGCCGTCGCCGATTTCTTCACCG
ACGAACTGCTGCGCAACGGCACGACCACCGCAATGGTCTGGAGCACCGTGCACGCGGCATCGGCCGACGCACTGTTCGCC
GCCAGCCAGCAACGCCACATGCGGATGATCACCGGCAAGGTGCTGATGGATCGCAACTGCCCGGACTTCCTGCGCGATAC
AGCCGAGAGCGGCGCGCGTGAATCGGCCGACCTGATCTCGAAATGGCACAACGTCGATCGCCTGGCCTACGCGATCACGC
CGCGTTTCGCACCGACTTCCACCGAGGCTCAATTGGAAGCCTGCGGCGAGTTGGCGAAGGCCCACAGCGACGTCTTCATC
CAGACCCACGTGGCGGAAAACCGCGACGAGGTGAAATGGGTGGCCGAGCTGTTCCCCGATGCGCGCAGCTACCTGGACGT
GTACGACCGCTACAAGCTCCTGCGCCCGGGCGCGTTCTACGGCCACGCGATCTACCTCGACGAGGATGACCGCCGCCGTC
TGGCCGACAGCGGCGCGGCCGTGGCTCACTGCCCCACGTCGAACCTGTTCCTGGGCAGCGGCTTCTACGACTTCCACCAG
TCGGATGCGAATCGCCTGAACGTCACGCTGGCGACGGACGTTGGCGGCGGCACGTCGTTCTCGATGTTCCGCACCATGAA
CGCGGCGCACAAGGTGGCGCGGATGGGCGGCTACCACCTGACGGCACTACGCATGTTCTACCTGGCCACGCGCGCCGCTG
CGGAGGCGCTCGGCTGGCAGGACCGGATCGGCAGCTTCGAAGTGGGCTGCGAGGCCGACTTCGTCGTGCTGGACCCGGAC
GCCACCCCGCTGATTTCCCGCCGCAGCAGCCGCTCGGAAACGCTTGAAGAGCAGTTGTTCGCGTTCGCGATGCTTGGCGA
CGACCGGGTCATCGAGAACGTCTACATCATGGGCGAAGCCACTAGCCCGGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
CCAACGTGACAACCACGACCTGACCGGGCGCATTCCAAGAACGCTGGATGCTTCGCCCGTTGCAATACCGAACGGGCGAG
GCACCGGCCGCCCCGAAGAC

Downstream 100 bases:

>100_bases
TCGGACTTAGGCGGGGCATGTTTGTGTGGATCGGCCGATTCCACCCAAACATGGTCCGTCGATGTGCTTTTTTGGTCTCA
ATGCGAATCCGCCCTCGCGG

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKMTTTSPASGADNAVTRAIRGRILHFLRDPQFHDDAYQYWEDGLMIVKAGRIAAVGNYADLSRDIPQGAEVVDHRGKLI
VPGFIDTHIHYPQTDIIASPSPGLLHWLDTYTFPEERRFSDPAYARAVADFFTDELLRNGTTTAMVWSTVHAASADALFA
ASQQRHMRMITGKVLMDRNCPDFLRDTAESGARESADLISKWHNVDRLAYAITPRFAPTSTEAQLEACGELAKAHSDVFI
QTHVAENRDEVKWVAELFPDARSYLDVYDRYKLLRPGAFYGHAIYLDEDDRRRLADSGAAVAHCPTSNLFLGSGFYDFHQ
SDANRLNVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHLTALRMFYLATRAAAEALGWQDRIGSFEVGCEADFVVLDPD
ATPLISRRSSRSETLEEQLFAFAMLGDDRVIENVYIMGEATSPVAA

Sequences:

>Translated_446_residues
MKMTTTSPASGADNAVTRAIRGRILHFLRDPQFHDDAYQYWEDGLMIVKAGRIAAVGNYADLSRDIPQGAEVVDHRGKLI
VPGFIDTHIHYPQTDIIASPSPGLLHWLDTYTFPEERRFSDPAYARAVADFFTDELLRNGTTTAMVWSTVHAASADALFA
ASQQRHMRMITGKVLMDRNCPDFLRDTAESGARESADLISKWHNVDRLAYAITPRFAPTSTEAQLEACGELAKAHSDVFI
QTHVAENRDEVKWVAELFPDARSYLDVYDRYKLLRPGAFYGHAIYLDEDDRRRLADSGAAVAHCPTSNLFLGSGFYDFHQ
SDANRLNVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHLTALRMFYLATRAAAEALGWQDRIGSFEVGCEADFVVLDPD
ATPLISRRSSRSETLEEQLFAFAMLGDDRVIENVYIMGEATSPVAA
>Mature_446_residues
MKMTTTSPASGADNAVTRAIRGRILHFLRDPQFHDDAYQYWEDGLMIVKAGRIAAVGNYADLSRDIPQGAEVVDHRGKLI
VPGFIDTHIHYPQTDIIASPSPGLLHWLDTYTFPEERRFSDPAYARAVADFFTDELLRNGTTTAMVWSTVHAASADALFA
ASQQRHMRMITGKVLMDRNCPDFLRDTAESGARESADLISKWHNVDRLAYAITPRFAPTSTEAQLEACGELAKAHSDVFI
QTHVAENRDEVKWVAELFPDARSYLDVYDRYKLLRPGAFYGHAIYLDEDDRRRLADSGAAVAHCPTSNLFLGSGFYDFHQ
SDANRLNVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHLTALRMFYLATRAAAEALGWQDRIGSFEVGCEADFVVLDPD
ATPLISRRSSRSETLEEQLFAFAMLGDDRVIENVYIMGEATSPVAA

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=383, Percent_Identity=34.9869451697128, Blast_Score=222, Evalue=6e-58,
Organism=Escherichia coli, GI1789249, Length=424, Percent_Identity=46.4622641509434, Blast_Score=421, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319963, Length=354, Percent_Identity=31.638418079096, Blast_Score=179, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24643849, Length=434, Percent_Identity=31.7972350230415, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 49566; Mature: 49566

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTTTSPASGADNAVTRAIRGRILHFLRDPQFHDDAYQYWEDGLMIVKAGRIAAVGNYA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEECCCEEEECCHH
DLSRDIPQGAEVVDHRGKLIVPGFIDTHIHYPQTDIIASPSPGLLHWLDTYTFPEERRFS
HHHCCCCCCHHHHHCCCCEEECCHHHCCCCCCCCCEECCCCCCHHHHHHHCCCCHHHCCC
DPAYARAVADFFTDELLRNGTTTAMVWSTVHAASADALFAASQQRHMRMITGKVLMDRNC
CHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCC
PDFLRDTAESGARESADLISKWHNVDRLAYAITPRFAPTSTEAQLEACGELAKAHSDVFI
HHHHHHHHHCCCHHHHHHHHHHHCHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEE
QTHVAENRDEVKWVAELFPDARSYLDVYDRYKLLRPGAFYGHAIYLDEDDRRRLADSGAA
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEEEECCCHHHHHHCCCCE
VAHCPTSNLFLGSGFYDFHQSDANRLNVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHL
EEECCCCCEEEECCCCHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHH
TALRMFYLATRAAAEALGWQDRIGSFEVGCEADFVVLDPDATPLISRRSSRSETLEEQLF
HHHHHHHHHHHHHHHHCCHHHCCCCEEECCCCEEEEECCCCCCHHHCCCCHHHHHHHHHH
AFAMLGDDRVIENVYIMGEATSPVAA
HHHHCCCCCEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MKMTTTSPASGADNAVTRAIRGRILHFLRDPQFHDDAYQYWEDGLMIVKAGRIAAVGNYA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEECCCEEEECCHH
DLSRDIPQGAEVVDHRGKLIVPGFIDTHIHYPQTDIIASPSPGLLHWLDTYTFPEERRFS
HHHCCCCCCHHHHHCCCCEEECCHHHCCCCCCCCCEECCCCCCHHHHHHHCCCCHHHCCC
DPAYARAVADFFTDELLRNGTTTAMVWSTVHAASADALFAASQQRHMRMITGKVLMDRNC
CHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCC
PDFLRDTAESGARESADLISKWHNVDRLAYAITPRFAPTSTEAQLEACGELAKAHSDVFI
HHHHHHHHHCCCHHHHHHHHHHHCHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEE
QTHVAENRDEVKWVAELFPDARSYLDVYDRYKLLRPGAFYGHAIYLDEDDRRRLADSGAA
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEEEECCCHHHHHHCCCCE
VAHCPTSNLFLGSGFYDFHQSDANRLNVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHL
EEECCCCCEEEECCCCHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHH
TALRMFYLATRAAAEALGWQDRIGSFEVGCEADFVVLDPDATPLISRRSSRSETLEEQLF
HHHHHHHHHHHHHHHHCCHHHCCCCEEECCCCEEEEECCCCCCHHHCCCCHHHHHHHHHH
AFAMLGDDRVIENVYIMGEATSPVAA
HHHHCCCCCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]