Click here to switch to the map view.

The map label for this gene is add

Identifier: 94309835

GI number: 94309835

Start: 975017

End: 976081

Strand: Reverse

Name: add

Synonym: Rmet_0890

Alternate gene names: 94309835

Gene position: 976081-975017 (Counterclockwise)

Preceding gene: 94309836

Following gene: 94309834

Centisome position: 24.85

GC content: 64.6

Gene sequence:

>1065_bases
ATGACCATCGATGCCGCGCTGGCGGACAAGATCCGCCGTACCCCCAAGGCCGAACTGCACGTGCATATCGAAGGCACGCT
CGAACCCGAGCGCATCTTCAGGCTGGCCCAGCGCAACAACGTCAAGCTGGCCTATCCGGACGTGGAAGCGCTACGCGCCG
CGTACGCCTTCACGGACCTGCAATCGTTTCTGGACATCTACTACGCCGGCGCCAGCGTGCTGCTGACCGAAGAAGATTTC
TTCGACATGACGATGGACTACGTCAAGCGCGCCGCCGCCGACAACGTCCGCCATGCCGAGATCTTCTTCGACCCGCAGAC
CCACACCGCGCGTGGTGTGCCGATGGGCACGGTGATCGACGGCATCGCCGACGCACTGGCTCAGGCACGCACCGAGTACG
ATTTTTCGAGCAGCATGATCCTCTGCTTCCTGCGCCACCTGTCTGAGGAGGATGCGCTGGCCACGCTGGAGGCGGCACTG
CCCTATCGCGACCGCTTTGTCGGCGTGGGACTGGATTCTTCGGAACGCGGCAACCCGCCCGAGAAGTTCGCGCGCGTGTT
TGCCCGCGCGAAGGAACTCGGCCTGCATCTGGTGGCGCACGCTGGCGAGGAAGGCCCGCCGCAGTACGTGACCGATGCAC
TGGACATCCTCAAGGTCGAGCGGATCGACCACGGCGTGCGCGCGATCGAGGACGAGGCGCTGGTGCAGCGCCTGGCACGT
GAACGCGTGGCGCTGACCGTTTGCCCGCTGTCGAACCAGAAGCTGATGGTCCACCCCGACCTGCGCGATCACCCGATGAA
GCGTCTGCTCGACGCCGGTGTGGCCGTGACGCTCCATTCGGACGACCCCGCCTATTTTGGCGGCTATATGAATGCGAACT
GGGAGGCATCGTTCGACGCACTCCCGCTCGATGCGGCCGATGCCCACAAGCTCGCACGCAACAGTTTTGAAGCGGCCTTC
CTGCCGGACGTGCAGAAGGCCGAATTCCTGGCTGAAGTCGACCACTTCTGGTCGGCCACGCCCGCATCCCCGCCCGCGAC
GGCCAACGTGACAACCACGACCTGA

Upstream 100 bases:

>100_bases
GCGACCAGCGCGCAGCCGCGCTGGCCGCCAGCCGGCGGGAGGCGGTGCATCCGTAACTGGGCAGCCGTGATCCGGCTGCC
GCCATAAGACAAGAGGCCCC

Downstream 100 bases:

>100_bases
CCGGGCGCATTCCAAGAACGCTGGATGCTTCGCCCGTTGCAATACCGAACGGGCGAGGCACCGGCCGCCCCGAAGACATG
AAGATGACGACGACTTCCCC

Product: adenosine deaminase

Products: NA

Alternate protein names: Adenosine aminohydrolase

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTIDAALADKIRRTPKAELHVHIEGTLEPERIFRLAQRNNVKLAYPDVEALRAAYAFTDLQSFLDIYYAGASVLLTEEDF
FDMTMDYVKRAAADNVRHAEIFFDPQTHTARGVPMGTVIDGIADALAQARTEYDFSSSMILCFLRHLSEEDALATLEAAL
PYRDRFVGVGLDSSERGNPPEKFARVFARAKELGLHLVAHAGEEGPPQYVTDALDILKVERIDHGVRAIEDEALVQRLAR
ERVALTVCPLSNQKLMVHPDLRDHPMKRLLDAGVAVTLHSDDPAYFGGYMNANWEASFDALPLDAADAHKLARNSFEAAF
LPDVQKAEFLAEVDHFWSATPASPPATANVTTTT

Sequences:

>Translated_354_residues
MTIDAALADKIRRTPKAELHVHIEGTLEPERIFRLAQRNNVKLAYPDVEALRAAYAFTDLQSFLDIYYAGASVLLTEEDF
FDMTMDYVKRAAADNVRHAEIFFDPQTHTARGVPMGTVIDGIADALAQARTEYDFSSSMILCFLRHLSEEDALATLEAAL
PYRDRFVGVGLDSSERGNPPEKFARVFARAKELGLHLVAHAGEEGPPQYVTDALDILKVERIDHGVRAIEDEALVQRLAR
ERVALTVCPLSNQKLMVHPDLRDHPMKRLLDAGVAVTLHSDDPAYFGGYMNANWEASFDALPLDAADAHKLARNSFEAAF
LPDVQKAEFLAEVDHFWSATPASPPATANVTTTT
>Mature_353_residues
TIDAALADKIRRTPKAELHVHIEGTLEPERIFRLAQRNNVKLAYPDVEALRAAYAFTDLQSFLDIYYAGASVLLTEEDFF
DMTMDYVKRAAADNVRHAEIFFDPQTHTARGVPMGTVIDGIADALAQARTEYDFSSSMILCFLRHLSEEDALATLEAALP
YRDRFVGVGLDSSERGNPPEKFARVFARAKELGLHLVAHAGEEGPPQYVTDALDILKVERIDHGVRAIEDEALVQRLARE
RVALTVCPLSNQKLMVHPDLRDHPMKRLLDAGVAVTLHSDDPAYFGGYMNANWEASFDALPLDAADAHKLARNSFEAAFL
PDVQKAEFLAEVDHFWSATPASPPATANVTTTT

Specific function: Unknown

COG id: COG1816

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenosine and AMP deaminases family

Homologues:

Organism=Homo sapiens, GI47078295, Length=356, Percent_Identity=24.7191011235955, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1787910, Length=317, Percent_Identity=28.0757097791798, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17538512, Length=336, Percent_Identity=24.702380952381, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32565969, Length=336, Percent_Identity=24.702380952381, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324188, Length=333, Percent_Identity=43.5435435435435, Blast_Score=283, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ADD_RALME (Q1LQ00)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_583045.1
- ProteinModelPortal:   Q1LQ00
- SMR:   Q1LQ00
- STRING:   Q1LQ00
- GeneID:   4037686
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0890
- eggNOG:   COG1816
- HOGENOM:   HBG630382
- OMA:   YFGGYLN
- PhylomeDB:   Q1LQ00
- ProtClustDB:   PRK09358
- BioCyc:   RMET266264:RMET_0890-MONOMER
- HAMAP:   MF_00540
- InterPro:   IPR001365
- InterPro:   IPR006330
- PANTHER:   PTHR11409
- TIGRFAMs:   TIGR01430

Pfam domain/function: PF00962 A_deaminase

EC number: =3.5.4.4

Molecular weight: Translated: 39235; Mature: 39103

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00485 A_DEAMINASE

Important sites: ACT_SITE 203-203 BINDING 22-22 BINDING 24-24 BINDING 282-282

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDAALADKIRRTPKAELHVHIEGTLEPERIFRLAQRNNVKLAYPDVEALRAAYAFTDL
CCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHH
QSFLDIYYAGASVLLTEEDFFDMTMDYVKRAAADNVRHAEIFFDPQTHTARGVPMGTVID
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHH
GIADALAQARTEYDFSSSMILCFLRHLSEEDALATLEAALPYRDRFVGVGLDSSERGNPP
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCH
EKFARVFARAKELGLHLVAHAGEEGPPQYVTDALDILKVERIDHGVRAIEDEALVQRLAR
HHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERVALTVCPLSNQKLMVHPDLRDHPMKRLLDAGVAVTLHSDDPAYFGGYMNANWEASFDA
HCEEEEEECCCCCEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCC
LPLDAADAHKLARNSFEAAFLPDVQKAEFLAEVDHFWSATPASPPATANVTTTT
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
TIDAALADKIRRTPKAELHVHIEGTLEPERIFRLAQRNNVKLAYPDVEALRAAYAFTDL
CCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHH
QSFLDIYYAGASVLLTEEDFFDMTMDYVKRAAADNVRHAEIFFDPQTHTARGVPMGTVID
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHH
GIADALAQARTEYDFSSSMILCFLRHLSEEDALATLEAALPYRDRFVGVGLDSSERGNPP
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCH
EKFARVFARAKELGLHLVAHAGEEGPPQYVTDALDILKVERIDHGVRAIEDEALVQRLAR
HHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERVALTVCPLSNQKLMVHPDLRDHPMKRLLDAGVAVTLHSDDPAYFGGYMNANWEASFDA
HCEEEEEECCCCCEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCC
LPLDAADAHKLARNSFEAAFLPDVQKAEFLAEVDHFWSATPASPPATANVTTTT
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA