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The map label for this gene is purD [H]

Identifier: 94309729

GI number: 94309729

Start: 860063

End: 861331

Strand: Reverse

Name: purD [H]

Synonym: Rmet_0784

Alternate gene names: 94309729

Gene position: 861331-860063 (Counterclockwise)

Preceding gene: 94309730

Following gene: 94309728

Centisome position: 21.93

GC content: 66.27

Gene sequence:

>1269_bases
ATGAAAGTTTTGGTTGTCGGCTCGGGTGGACGTGAACACGCGCTGGCGTGGAAGTTGGCCCAGTCCCCCAAGGTGCAAGT
CGTGTACGTGGCACCGGGCAACGGCGGGACGGCGCTGGACAAGCGTCTGCAGAACGTGCCGCTGACCGACCCGGAGGTGC
TGGCCGCCTTCGTCGAGCGTGAAGGTGTGGCGTTCACGGTGGTTGGCCCCGAGGCGCCACTGGCTGGCGGGATCGTCGAC
GTGTTCCGCGCCCGCGGCCTGCGTATCTTCGGCCCGACCAAGGCCGCCGCCCAGCTGGAATCGTCGAAGGACTTCGCCAA
GGCCTTCATGCACCGCCATGGCATTCCCACGGCCTATTACCAGACGTTCTCCGACGCTGCCGGCGCCCACGCCTATATCG
ACGCCCAAGGCGCGCCAATCGTGATCAAGGCCGACGGCCTGGCCGCGGGCAAGGGCGTGGTGGTGGCGATGACGCTGGAA
GAAGCGCACGCTGCGGTCGACATGATGCTGGCCGACAACAAGCTCGGCGATGCCGGCGCCCGGGTCGTGATCGAGGAATT
CCTCGAAGGCGAGGAAGCCAGCTTCATCGTGATGGTCGACGGCAAGAACGTGCTGGCGCTGGCCACTAGTCAGGACCACA
AGCGTCTGCTGGATGGCGACGCGGGCCCCAACACCGGCGGCATGGGCGCGTACTCGCCTGCCCCCGTGGTCACGCCCGCG
CTCCATGCGCGCGCGCTGCGCGAAATTATCCTGCCGACCGTGCGTGGCATGGAAAAGGACGGCATCCCGTTCACGGGCTT
CCTGTATGCCGGCCTGATGATCGACGCCAACGGCAACCCGAAGACGCTGGAATTCAACTGCCGCATGGGCGATCCGGAAA
CGCAGCCGATCATGGCGCGCCTGAAGACCGACCTGGTGGATGTGATGGAAGCGGCCGTGGACGGCAAGCTGGATCAGATC
GAGCTGGACTGGGATCGCCGCACCGCGCTGGGCGTGGTCATGGCCGCCTACGACTACCCGGAAAACCCGCGCAAGGGCGA
TGCGATCACCGGCATCCCCGCGGAAACCGCCGACGCCGTGACGTTCCACGCCGGCACCACGCTCAAGGATGGCACGCTGC
TGACCTCCGGCGGCCGCGTGCTCTGCGTGGTGGGCCTGGCCGATACGGTCAAGGCAGCACAGAAGAACGCCTACGCCGCA
GTGGAACAGATCCGCTTCGACGGGATGCAATTCCGCACCGACATCGGATACCGCGCCATCAAGCGTTGA

Upstream 100 bases:

>100_bases
GGTGGTCGAGGAATAAGCCTCTCGCGCCGCGCCCCATGGCGGCAGCCCGGCACCAGCCAGCTGCCGCTTTTCGCATGCGT
TTCTTTTCTGGCAGTGGATC

Downstream 100 bases:

>100_bases
TGACCCCAGGTATCCGACCATTCGCCCCACGACACGTGGGGCGAAGCCAGCCGGTACAATCATGACACCGCAATGACAGC
CGGAGCGCCCGCCGCCCGGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKVLVVGSGGREHALAWKLAQSPKVQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFVEREGVAFTVVGPEAPLAGGIVD
VFRARGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAYYQTFSDAAGAHAYIDAQGAPIVIKADGLAAGKGVVVAMTLE
EAHAAVDMMLADNKLGDAGARVVIEEFLEGEEASFIVMVDGKNVLALATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPA
LHARALREIILPTVRGMEKDGIPFTGFLYAGLMIDANGNPKTLEFNCRMGDPETQPIMARLKTDLVDVMEAAVDGKLDQI
ELDWDRRTALGVVMAAYDYPENPRKGDAITGIPAETADAVTFHAGTTLKDGTLLTSGGRVLCVVGLADTVKAAQKNAYAA
VEQIRFDGMQFRTDIGYRAIKR

Sequences:

>Translated_422_residues
MKVLVVGSGGREHALAWKLAQSPKVQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFVEREGVAFTVVGPEAPLAGGIVD
VFRARGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAYYQTFSDAAGAHAYIDAQGAPIVIKADGLAAGKGVVVAMTLE
EAHAAVDMMLADNKLGDAGARVVIEEFLEGEEASFIVMVDGKNVLALATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPA
LHARALREIILPTVRGMEKDGIPFTGFLYAGLMIDANGNPKTLEFNCRMGDPETQPIMARLKTDLVDVMEAAVDGKLDQI
ELDWDRRTALGVVMAAYDYPENPRKGDAITGIPAETADAVTFHAGTTLKDGTLLTSGGRVLCVVGLADTVKAAQKNAYAA
VEQIRFDGMQFRTDIGYRAIKR
>Mature_422_residues
MKVLVVGSGGREHALAWKLAQSPKVQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFVEREGVAFTVVGPEAPLAGGIVD
VFRARGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAYYQTFSDAAGAHAYIDAQGAPIVIKADGLAAGKGVVVAMTLE
EAHAAVDMMLADNKLGDAGARVVIEEFLEGEEASFIVMVDGKNVLALATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPA
LHARALREIILPTVRGMEKDGIPFTGFLYAGLMIDANGNPKTLEFNCRMGDPETQPIMARLKTDLVDVMEAAVDGKLDQI
ELDWDRRTALGVVMAAYDYPENPRKGDAITGIPAETADAVTFHAGTTLKDGTLLTSGGRVLCVVGLADTVKAAQKNAYAA
VEQIRFDGMQFRTDIGYRAIKR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=50.2358490566038, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=50.2358490566038, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=50.2358490566038, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=50.2358490566038, Blast_Score=405, Evalue=1e-113,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=63.3802816901408, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=43.9429928741093, Blast_Score=334, Evalue=6e-92,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=44.4943820224719, Blast_Score=343, Evalue=3e-95,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=48.6111111111111, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=48.6111111111111, Blast_Score=356, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44715; Mature: 44715

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVVGSGGREHALAWKLAQSPKVQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFVER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHC
EGVAFTVVGPEAPLAGGIVDVFRARGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAYY
CCEEEEEECCCCCCCCHHHHHHHHCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCHHHH
QTFSDAAGAHAYIDAQGAPIVIKADGLAAGKGVVVAMTLEEAHAAVDMMLADNKLGDAGA
HHHHCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEEEHHHHHHEEEEEECCCCCCCHH
RVVIEEFLEGEEASFIVMVDGKNVLALATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPA
HHHHHHHHCCCCCEEEEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARALREIILPTVRGMEKDGIPFTGFLYAGLMIDANGNPKTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKTDLVDVMEAAVDGKLDQIELDWDRRTALGVVMAAYDYPENPRKGDAITGIPAETADAV
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHEEEEEEECCCCCCCCCCEEECCCCCCCCEE
TFHAGTTLKDGTLLTSGGRVLCVVGLADTVKAAQKNAYAAVEQIRFDGMQFRTDIGYRAI
EEECCCEECCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHH
KR
CC
>Mature Secondary Structure
MKVLVVGSGGREHALAWKLAQSPKVQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFVER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHC
EGVAFTVVGPEAPLAGGIVDVFRARGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAYY
CCEEEEEECCCCCCCCHHHHHHHHCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCHHHH
QTFSDAAGAHAYIDAQGAPIVIKADGLAAGKGVVVAMTLEEAHAAVDMMLADNKLGDAGA
HHHHCCCCCEEEEECCCCEEEEEECCCCCCCCEEEEEEEHHHHHHEEEEEECCCCCCCHH
RVVIEEFLEGEEASFIVMVDGKNVLALATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPA
HHHHHHHHCCCCCEEEEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARALREIILPTVRGMEKDGIPFTGFLYAGLMIDANGNPKTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKTDLVDVMEAAVDGKLDQIELDWDRRTALGVVMAAYDYPENPRKGDAITGIPAETADAV
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHEEEEEEECCCCCCCCCCEEECCCCCCCCEE
TFHAGTTLKDGTLLTSGGRVLCVVGLADTVKAAQKNAYAAVEQIRFDGMQFRTDIGYRAI
EEECCCEECCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHH
KR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]