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The map label for this gene is nadE [H]

Identifier: 94309625

GI number: 94309625

Start: 752164

End: 754314

Strand: Direct

Name: nadE [H]

Synonym: Rmet_0680

Alternate gene names: 94309625

Gene position: 752164-754314 (Clockwise)

Preceding gene: 94309623

Following gene: 94309626

Centisome position: 19.15

GC content: 65.23

Gene sequence:

>2151_bases
ATGCTGCGGCGCAGGATGCGGGGCAGCACGCGCCGCGTGTGCGTCGTAGAATGCAGGCATCCTCAGCCCCCCAATCCTGG
GCCATCCATGACTCACCCGTTCTTCAACCTCTATTCCCACGGCTTCGCGCGCGTGGCCGTGGGCGTGCCCGAGTGCCGCG
TGGCCGATCCGGCCTTCAATGCCGAGCAGACGATCCTGCTGGCGCGCCAGGCGGCCGACGGCGGCGCCGTGCTGGTGGCG
TTTCCGGAACTGGGCCTGTCGGCTTACACGTGCGATGACCTGTTTCACCAGCGGGCGCTGCTGCGTGAATGCGAACTGGC
GCTGCAACGGATCGTGGAGGCGTCGGCCGGGATCAACGCGGCGCTGATCGTCGGCCTGCCGGTACTGGTGGAGCACCAGC
TGTTCAACTGCGCAGTGGTTGTGGCGGGCGGCAAGATTCAGGGCGTGGTGCCCAAGACCTATCTGCCCAACTACTGGGAA
TTCTATGAGGCACGCCAGTTCAGCTCCGCTGACTGCGCGGCCACGCCGCTGGTCTCGCTGCTTGGCGCGGAGGTGCCGTT
CGGCGCCGATCTGCTGTTCGAGATCGAGAGCATTCCTTTCTTCCGCTTCCACGCGGAGATCTGCGAGGACGTCTGGGTGC
CGATTCCCCCGTCGTCGTTTGCCGCGCTGGCCGGCGCCACCGTGCTCGTGAACCTGTCGGCATCGAATATCGTGATTGGC
AAGTCGGGGTATCGCCATCAACTCGTGTCGCAGCAGTCGGCACGCTGCCTGGCCGCGTACCTGTACACGTCCGCCGGCAA
GGGCGAATCGACGACCGATCTGGCCTGGGATGGCCAGGCGTTGATCTATGAAAACGGTGAACTGCTGGGCGAATCGGAAC
GGTTTGCCGACAGTTCCCACCTGCTGTTCGCCGATGTCGACCTCGAGCGGTTGTCGCGCGAGCGGATGCACCAGACGTCG
TTTGGTCAGTCCGTGCGCCGCCACCACGAGGAAGTGGAACGCTTCGACACCGTGTCGTTCCACATCGACGTGCCGTCCAC
GCAGGCCCTGCCGCTGCAGCGCAAGGTGGCGCGCTTCCCGTATGTGCCGTCGGACCCGCGCCAGCGCGATGAGCGCTGCA
TGGAGGTCTACAACATCCAGGTGCAGGCGCTGGTGCAGCGGCTGTCGTCGAGCAAGATCTCGAAGGTGGTGATCGGCGTC
TCGGGCGGGCTCGATTCCACCCATGCCCTGCTGGTGTGCGCCAAGGCGATGGATCGGCTCGGCCTGCCTCGCTCGAACAT
CCTGGCCTATACGATGCCAGGCTTTGCCACGAGCGACCGCACGCTGCAGCAGGCGCGCGAATTGATGAAACTGGTGGGCT
GCACGGCGCGCGAGATCGATATCCGGCCGAGTTGCCTGGCAATGCTCGCGGATCTGGATCATCCGTATTCGCGCGGCGAA
CCGGTCTACGACGTCACGTTCGAAAACGTGCAGGCCGGTGAGCGCACCAACCATCTGTTCCGGCTGGCGAATCACAACCA
TGCGATCGTGATCGGCACCGGCGATCTCAGCGAGCTGGCGCTGGGCTGGTGTACCTACGGCGTGGGCGACCACATGTCGC
ACTACAACGTGAACGCCAGTGTGCCCAAGACGCTGATCAGCCACCTGGTGCGCTGGGTGGCCGAGACCGGGCAGGTGGCC
GAGGGCGGCCAGGATGTGCTGCTGGCGGTGCTGGCCACCGACATCAGCCCGGAACTGGTGCCCAGCGGGGCAACCGCCGC
CGGGCAGGACAAGGCACCCGAGCAGAAGACCGAGCAGATTATCGGGCCGTACGAGTTGCAGGATTTCAACCTGTATTACA
CGCTGCGCTTCGGGTTCACGCCGTCGAAGGTGGCATTCCTGGCACTTCATGCCTGGGGTGACGCGGCGCGTGGAACGTGG
CCGAACGGACCTCATGTGGCCCGCAACGTCTATCAACTGCCCGAAATCAAGCGCGTCCTCGGTATCTTTGTCGACCGCTT
CTTCCGCACCAGCCAGTTCAAGCGCTCCTGTATTCCTAACGCGCCCAAGGTCGGCTCGGGCGGCTCGCTCTCCCCGCGCG
GCGACTGGCGCGCGCCGAGCGATGGCGAGTCGGTCGTGTGGATGCGGGATCTCGCGCGGATCCCGGATTGA

Upstream 100 bases:

>100_bases
CGGTAGTTGGGGCTGAGGCGCGATTCGCTCATGAATACTGGGGAGGGCGGGGGAGGGCAGGTTGTCCACGGCCAGATGCT
GATCGAGTCTACCGGAAACG

Downstream 100 bases:

>100_bases
ACCGGCAGAAATCGGGTGCGATTGCGGGCAATGTGGGTCTGCACGCAATCTTCATGTGGTGTTTCGGGCTAGAATCCTTC
ATCTTTCGACATATTTAGAG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MLRRRMRGSTRRVCVVECRHPQPPNPGPSMTHPFFNLYSHGFARVAVGVPECRVADPAFNAEQTILLARQAADGGAVLVA
FPELGLSAYTCDDLFHQRALLRECELALQRIVEASAGINAALIVGLPVLVEHQLFNCAVVVAGGKIQGVVPKTYLPNYWE
FYEARQFSSADCAATPLVSLLGAEVPFGADLLFEIESIPFFRFHAEICEDVWVPIPPSSFAALAGATVLVNLSASNIVIG
KSGYRHQLVSQQSARCLAAYLYTSAGKGESTTDLAWDGQALIYENGELLGESERFADSSHLLFADVDLERLSRERMHQTS
FGQSVRRHHEEVERFDTVSFHIDVPSTQALPLQRKVARFPYVPSDPRQRDERCMEVYNIQVQALVQRLSSSKISKVVIGV
SGGLDSTHALLVCAKAMDRLGLPRSNILAYTMPGFATSDRTLQQARELMKLVGCTAREIDIRPSCLAMLADLDHPYSRGE
PVYDVTFENVQAGERTNHLFRLANHNHAIVIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLISHLVRWVAETGQVA
EGGQDVLLAVLATDISPELVPSGATAAGQDKAPEQKTEQIIGPYELQDFNLYYTLRFGFTPSKVAFLALHAWGDAARGTW
PNGPHVARNVYQLPEIKRVLGIFVDRFFRTSQFKRSCIPNAPKVGSGGSLSPRGDWRAPSDGESVVWMRDLARIPD

Sequences:

>Translated_716_residues
MLRRRMRGSTRRVCVVECRHPQPPNPGPSMTHPFFNLYSHGFARVAVGVPECRVADPAFNAEQTILLARQAADGGAVLVA
FPELGLSAYTCDDLFHQRALLRECELALQRIVEASAGINAALIVGLPVLVEHQLFNCAVVVAGGKIQGVVPKTYLPNYWE
FYEARQFSSADCAATPLVSLLGAEVPFGADLLFEIESIPFFRFHAEICEDVWVPIPPSSFAALAGATVLVNLSASNIVIG
KSGYRHQLVSQQSARCLAAYLYTSAGKGESTTDLAWDGQALIYENGELLGESERFADSSHLLFADVDLERLSRERMHQTS
FGQSVRRHHEEVERFDTVSFHIDVPSTQALPLQRKVARFPYVPSDPRQRDERCMEVYNIQVQALVQRLSSSKISKVVIGV
SGGLDSTHALLVCAKAMDRLGLPRSNILAYTMPGFATSDRTLQQARELMKLVGCTAREIDIRPSCLAMLADLDHPYSRGE
PVYDVTFENVQAGERTNHLFRLANHNHAIVIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLISHLVRWVAETGQVA
EGGQDVLLAVLATDISPELVPSGATAAGQDKAPEQKTEQIIGPYELQDFNLYYTLRFGFTPSKVAFLALHAWGDAARGTW
PNGPHVARNVYQLPEIKRVLGIFVDRFFRTSQFKRSCIPNAPKVGSGGSLSPRGDWRAPSDGESVVWMRDLARIPD
>Mature_716_residues
MLRRRMRGSTRRVCVVECRHPQPPNPGPSMTHPFFNLYSHGFARVAVGVPECRVADPAFNAEQTILLARQAADGGAVLVA
FPELGLSAYTCDDLFHQRALLRECELALQRIVEASAGINAALIVGLPVLVEHQLFNCAVVVAGGKIQGVVPKTYLPNYWE
FYEARQFSSADCAATPLVSLLGAEVPFGADLLFEIESIPFFRFHAEICEDVWVPIPPSSFAALAGATVLVNLSASNIVIG
KSGYRHQLVSQQSARCLAAYLYTSAGKGESTTDLAWDGQALIYENGELLGESERFADSSHLLFADVDLERLSRERMHQTS
FGQSVRRHHEEVERFDTVSFHIDVPSTQALPLQRKVARFPYVPSDPRQRDERCMEVYNIQVQALVQRLSSSKISKVVIGV
SGGLDSTHALLVCAKAMDRLGLPRSNILAYTMPGFATSDRTLQQARELMKLVGCTAREIDIRPSCLAMLADLDHPYSRGE
PVYDVTFENVQAGERTNHLFRLANHNHAIVIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLISHLVRWVAETGQVA
EGGQDVLLAVLATDISPELVPSGATAAGQDKAPEQKTEQIIGPYELQDFNLYYTLRFGFTPSKVAFLALHAWGDAARGTW
PNGPHVARNVYQLPEIKRVLGIFVDRFFRTSQFKRSCIPNAPKVGSGGSLSPRGDWRAPSDGESVVWMRDLARIPD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=594, Percent_Identity=23.5690235690236, Blast_Score=98, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538756, Length=279, Percent_Identity=28.3154121863799, Blast_Score=98, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321865, Length=305, Percent_Identity=25.9016393442623, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 78767; Mature: 78767

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRRMRGSTRRVCVVECRHPQPPNPGPSMTHPFFNLYSHGFARVAVGVPECRVADPAFN
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCEECCCCCC
AEQTILLARQAADGGAVLVAFPELGLSAYTCDDLFHQRALLRECELALQRIVEASAGINA
CCCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ALIVGLPVLVEHQLFNCAVVVAGGKIQGVVPKTYLPNYWEFYEARQFSSADCAATPLVSL
EEECCCHHHHHHHHHEEEEEEECCEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHH
LGAEVPFGADLLFEIESIPFFRFHAEICEDVWVPIPPSSFAALAGATVLVNLSASNIVIG
HCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEECCCEEEEE
KSGYRHQLVSQQSARCLAAYLYTSAGKGESTTDLAWDGQALIYENGELLGESERFADSSH
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCHHHCCCCCC
LLFADVDLERLSRERMHQTSFGQSVRRHHEEVERFDTVSFHIDVPSTQALPLQRKVARFP
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHCC
YVPSDPRQRDERCMEVYNIQVQALVQRLSSSKISKVVIGVSGGLDSTHALLVCAKAMDRL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
GLPRSNILAYTMPGFATSDRTLQQARELMKLVGCTAREIDIRPSCLAMLADLDHPYSRGE
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCCCCCC
PVYDVTFENVQAGERTNHLFRLANHNHAIVIGTGDLSELALGWCTYGVGDHMSHYNVNAS
CEEEEEECCCCCCCHHCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCC
VPKTLISHLVRWVAETGQVAEGGQDVLLAVLATDISPELVPSGATAAGQDKAPEQKTEQI
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHC
IGPYELQDFNLYYTLRFGFTPSKVAFLALHAWGDAARGTWPNGPHVARNVYQLPEIKRVL
CCCEEEEEEEEEEEEEECCCCHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GIFVDRFFRTSQFKRSCIPNAPKVGSGGSLSPRGDWRAPSDGESVVWMRDLARIPD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCC
>Mature Secondary Structure
MLRRRMRGSTRRVCVVECRHPQPPNPGPSMTHPFFNLYSHGFARVAVGVPECRVADPAFN
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCEECCCCCC
AEQTILLARQAADGGAVLVAFPELGLSAYTCDDLFHQRALLRECELALQRIVEASAGINA
CCCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ALIVGLPVLVEHQLFNCAVVVAGGKIQGVVPKTYLPNYWEFYEARQFSSADCAATPLVSL
EEECCCHHHHHHHHHEEEEEEECCEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHH
LGAEVPFGADLLFEIESIPFFRFHAEICEDVWVPIPPSSFAALAGATVLVNLSASNIVIG
HCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEECCCEEEEE
KSGYRHQLVSQQSARCLAAYLYTSAGKGESTTDLAWDGQALIYENGELLGESERFADSSH
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCHHHCCCCCC
LLFADVDLERLSRERMHQTSFGQSVRRHHEEVERFDTVSFHIDVPSTQALPLQRKVARFP
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHCC
YVPSDPRQRDERCMEVYNIQVQALVQRLSSSKISKVVIGVSGGLDSTHALLVCAKAMDRL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
GLPRSNILAYTMPGFATSDRTLQQARELMKLVGCTAREIDIRPSCLAMLADLDHPYSRGE
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCCCCCC
PVYDVTFENVQAGERTNHLFRLANHNHAIVIGTGDLSELALGWCTYGVGDHMSHYNVNAS
CEEEEEECCCCCCCHHCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCC
VPKTLISHLVRWVAETGQVAEGGQDVLLAVLATDISPELVPSGATAAGQDKAPEQKTEQI
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHC
IGPYELQDFNLYYTLRFGFTPSKVAFLALHAWGDAARGTWPNGPHVARNVYQLPEIKRVL
CCCEEEEEEEEEEEEEECCCCHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GIFVDRFFRTSQFKRSCIPNAPKVGSGGSLSPRGDWRAPSDGESVVWMRDLARIPD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]