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The map label for this gene is ppa [H]

Identifier: 94309623

GI number: 94309623

Start: 750286

End: 750813

Strand: Direct

Name: ppa [H]

Synonym: Rmet_0678

Alternate gene names: 94309623

Gene position: 750286-750813 (Clockwise)

Preceding gene: 94309618

Following gene: 94309625

Centisome position: 19.1

GC content: 61.17

Gene sequence:

>528_bases
ATGAGCTTCAACCTCGTCTCCCCGGGCAAGGACCTTCCCAACGATTTCAACGTCATCATCGAGATCTCGGCCCAGAGCGA
TCCCGTGAAGTACGAGGCCGACAAGGAGACCGGCCTGCTGTTCGTGGACCGCTTCATTGGTACCGGCATGCGCTACCCGG
CCAACTACGGCTTCATCCCGCAAACGCTGTCGGGCGACGGCGATCCGGTGGATGTGCTGGTCATCACCCCGTTCCCGATC
CTGGCCGGCGCCGTTGTGCGCTGCCGTGCACTGGGCATGCTGAAGATGACCGACGAGTCGGGCGTGGACGCCAAGCTCGT
GGCCATCCCCGTGGACAAGCTGTGCCCGGCTACCGCCCACATGAAGGGCCTGTCGGATGTGCCGCAAAATACGCTCGACC
AGATCAAGCACTTCTTCGAGAACTACAAGGCACTCGAAGCCGGCAAGTGGGTCAAGGTGGAAGGCTGGGCTGGCATCGAA
GAAGCCCACAAGGAAATCAGCGACGGCGTGGCGAACTTCAAGAAGTAA

Upstream 100 bases:

>100_bases
ACCGCGCGGGGCGTGCAAGGCACCCGGATGTCAGCCAGAGGTAAGAAGCAAACCGCTATAATGCCGGCCATCCCAACACA
CAGGCATTCGGAGAACTCTC

Downstream 100 bases:

>100_bases
TTCGCTGCCTGCCGCGTTATCGAAAAAACCCGCCATCTGGCGGGTTTTTTTGTTGCCGGGGGATGACTCGCTACTCGCGC
TACTCGCGCGCGCCCGCGAA

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSFNLVSPGKDLPNDFNVIIEISAQSDPVKYEADKETGLLFVDRFIGTGMRYPANYGFIPQTLSGDGDPVDVLVITPFPI
LAGAVVRCRALGMLKMTDESGVDAKLVAIPVDKLCPATAHMKGLSDVPQNTLDQIKHFFENYKALEAGKWVKVEGWAGIE
EAHKEISDGVANFKK

Sequences:

>Translated_175_residues
MSFNLVSPGKDLPNDFNVIIEISAQSDPVKYEADKETGLLFVDRFIGTGMRYPANYGFIPQTLSGDGDPVDVLVITPFPI
LAGAVVRCRALGMLKMTDESGVDAKLVAIPVDKLCPATAHMKGLSDVPQNTLDQIKHFFENYKALEAGKWVKVEGWAGIE
EAHKEISDGVANFKK
>Mature_174_residues
SFNLVSPGKDLPNDFNVIIEISAQSDPVKYEADKETGLLFVDRFIGTGMRYPANYGFIPQTLSGDGDPVDVLVITPFPIL
AGAVVRCRALGMLKMTDESGVDAKLVAIPVDKLCPATAHMKGLSDVPQNTLDQIKHFFENYKALEAGKWVKVEGWAGIEE
AHKEISDGVANFKK

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=175, Percent_Identity=59.4285714285714, Blast_Score=209, Evalue=9e-56,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 19084; Mature: 18953

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNLVSPGKDLPNDFNVIIEISAQSDPVKYEADKETGLLFVDRFIGTGMRYPANYGFIP
CCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCEEEEEHHHCCCCCCCCCCCCCC
QTLSGDGDPVDVLVITPFPILAGAVVRCRALGMLKMTDESGVDAKLVAIPVDKLCPATAH
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEECCCCCCCEEEEEECHHHHCCCHHH
MKGLSDVPQNTLDQIKHFFENYKALEAGKWVKVEGWAGIEEAHKEISDGVANFKK
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFNLVSPGKDLPNDFNVIIEISAQSDPVKYEADKETGLLFVDRFIGTGMRYPANYGFIP
CCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCEEEEEHHHCCCCCCCCCCCCCC
QTLSGDGDPVDVLVITPFPILAGAVVRCRALGMLKMTDESGVDAKLVAIPVDKLCPATAH
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEECCCCCCCEEEEEECHHHHCCCHHH
MKGLSDVPQNTLDQIKHFFENYKALEAGKWVKVEGWAGIEEAHKEISDGVANFKK
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]