Click here to switch to the map view.

The map label for this gene is hemL [H]

Identifier: 94309612

GI number: 94309612

Start: 734152

End: 735444

Strand: Reverse

Name: hemL [H]

Synonym: Rmet_0667

Alternate gene names: 94309612

Gene position: 735444-734152 (Counterclockwise)

Preceding gene: 94309619

Following gene: 94309610

Centisome position: 18.72

GC content: 67.98

Gene sequence:

>1293_bases
ATGTCCCGTAACCAGCAGCTTTTCGATCGCGCCCAGCAGACCATTCCCGGCGGCGTCAACTCGCCCGTCCGCGCCTTCCG
TTCGGTGGGCGGCACGCCGCGCTTTATCACCCGCGCCGAGGGTGCCTATATGTGGGATGCCGACGGCCAGCGCTATATCG
ACTACATCGGCTCCTGGGGCCCGATGATTGTCGGCCACGCGCATCCGGACGTGGTGCGCGCCGTGCAGGAGACCGCCACC
CAGAGTTTCTCGTTCGGCGCGCCGACCGAGGCCGAAATCGACATGGCCGAGGAAATCTGCAAGCTCGTGCCATCGATCGA
GCAGGTGCGCCTGGTGTCGTCGGGCACCGAAGCCACCATGAGCGCGCTGCGCCTGGCACGCGGCTTCACTGGCCGCGACC
TGATCATCAAGTTCGAGGGCTGCTACCACGGCCATGCCGACAGCCTGCTGGTCAAGGCCGGCTCGGGCCTGCTGACGTTT
GCCGACACCACGCAGAACGCGCCGTCGTCGGCGGGCGTGCCGGCGGATGTCACGCGGCACACGATGGTGCTGGAGTACAA
CAACGTCACCCAGCTCGAGGAAGCGTTCGCGCGCCACAAGGGCGAGATCGCCGCCGTGATCGTGGAGCCGGTGGCCGGCA
ACATGAACCTGGTGCGCGCCAGCGAGGCATTCCTGACCGCCATGCGCAACCTGTGCAGCGAGCACGGCAGCGTGCTGATC
TTCGACGAGGTGATGACGGGCTTCCGCGTGGCGCTTGGCGGCGCGCAGGCGCACTACGGCATCAAGCCGGACATGACCTG
CCTGGGCAAGGTGATCGGCGGCGGAATGCCGGCGGCGGCGTTCGGCGGCCGGCGCGACATCATGGCCAAGCTTGCGCCGC
TCGGTGGCGTCTATCAGGCAGGCACGCTCTCCGGCAACCCGCTGGCCGTGGCCGCCGGCCTGACCACGCTGCGCCTGATC
CAGGCCCCGGGCTTCTACGACCGACTGGCCACGCAGACGCGCAAGCTGGCCGACGGCCTGACCAAGGCCGCCCGTGAGGC
TGGCGTACCGTTCTGCGCGGACGCGATCGGCGGCATGTTCGGCATCTACTTCACCAACGAAGTGCCCGGCAGCTTTGCCG
AGGTGACCAAGGCCGACGTGGGCCGCTTCAACCGCTTCTTCCATGCAATGCTGGCCGAAGGCGTCTACCTGGCGCCGTCG
GCCTTCGAGGCCGGCTTCGTGTCGGCCAAGCATGACGATGCGATCATCGAGGCGACGGTGGCTGCCGCCGCCAAGGCCTT
CAAGGCTGGCTGA

Upstream 100 bases:

>100_bases
CGATTCACCCGATTTGCTCATATTTTGAGCATTTCGAAGCCGACGCGGCCCCCGGCCGGACGCATGTTTTTCCTCCTGAA
TCCTTTTCAGCCAGATTCCC

Downstream 100 bases:

>100_bases
CCGCCCCGCTCACGATATCCATCGCATCGAACATCGCCGCGAGCTGGTTCGCCAGTTCCGGCGATGCGCAGGTCATCGGC
GCGCGCAGTTCGTCGCGCAA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITRAEGAYMWDADGQRYIDYIGSWGPMIVGHAHPDVVRAVQETAT
QSFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTF
ADTTQNAPSSAGVPADVTRHTMVLEYNNVTQLEEAFARHKGEIAAVIVEPVAGNMNLVRASEAFLTAMRNLCSEHGSVLI
FDEVMTGFRVALGGAQAHYGIKPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQAGTLSGNPLAVAAGLTTLRLI
QAPGFYDRLATQTRKLADGLTKAAREAGVPFCADAIGGMFGIYFTNEVPGSFAEVTKADVGRFNRFFHAMLAEGVYLAPS
AFEAGFVSAKHDDAIIEATVAAAAKAFKAG

Sequences:

>Translated_430_residues
MSRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITRAEGAYMWDADGQRYIDYIGSWGPMIVGHAHPDVVRAVQETAT
QSFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTF
ADTTQNAPSSAGVPADVTRHTMVLEYNNVTQLEEAFARHKGEIAAVIVEPVAGNMNLVRASEAFLTAMRNLCSEHGSVLI
FDEVMTGFRVALGGAQAHYGIKPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQAGTLSGNPLAVAAGLTTLRLI
QAPGFYDRLATQTRKLADGLTKAAREAGVPFCADAIGGMFGIYFTNEVPGSFAEVTKADVGRFNRFFHAMLAEGVYLAPS
AFEAGFVSAKHDDAIIEATVAAAAKAFKAG
>Mature_429_residues
SRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITRAEGAYMWDADGQRYIDYIGSWGPMIVGHAHPDVVRAVQETATQ
SFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTFA
DTTQNAPSSAGVPADVTRHTMVLEYNNVTQLEEAFARHKGEIAAVIVEPVAGNMNLVRASEAFLTAMRNLCSEHGSVLIF
DEVMTGFRVALGGAQAHYGIKPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQAGTLSGNPLAVAAGLTTLRLIQ
APGFYDRLATQTRKLADGLTKAAREAGVPFCADAIGGMFGIYFTNEVPGSFAEVTKADVGRFNRFFHAMLAEGVYLAPSA
FEAGFVSAKHDDAIIEATVAAAAKAFKAG

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=332, Percent_Identity=26.8072289156627, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI24119277, Length=315, Percent_Identity=26.984126984127, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI284507298, Length=176, Percent_Identity=27.8409090909091, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786349, Length=428, Percent_Identity=59.1121495327103, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1789016, Length=367, Percent_Identity=31.8801089918256, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787560, Length=371, Percent_Identity=32.0754716981132, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788044, Length=356, Percent_Identity=30.3370786516854, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789759, Length=322, Percent_Identity=27.0186335403727, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI145693181, Length=316, Percent_Identity=31.0126582278481, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786991, Length=386, Percent_Identity=27.2020725388601, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=402, Percent_Identity=25.3731343283582, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=365, Percent_Identity=26.3013698630137, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI32564660, Length=322, Percent_Identity=26.7080745341615, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=275, Percent_Identity=24.7272727272727, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=317, Percent_Identity=27.7602523659306, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=333, Percent_Identity=26.7267267267267, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357415, Length=322, Percent_Identity=28.2608695652174, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21356575, Length=315, Percent_Identity=28.8888888888889, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45535; Mature: 45404

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITRAEGAYMWDADGQRYIDYIGSWG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCCEEECCCCHHHHHHHCCCC
PMIVGHAHPDVVRAVQETATQSFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATM
CEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
SALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTFADTTQNAPSSAGVPADVTRH
HHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCC
TMVLEYNNVTQLEEAFARHKGEIAAVIVEPVAGNMNLVRASEAFLTAMRNLCSEHGSVLI
EEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCEEE
FDEVMTGFRVALGGAQAHYGIKPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQA
EHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEC
GTLSGNPLAVAAGLTTLRLIQAPGFYDRLATQTRKLADGLTKAAREAGVPFCADAIGGMF
CCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCE
GIYFTNEVPGSFAEVTKADVGRFNRFFHAMLAEGVYLAPSAFEAGFVSAKHDDAIIEATV
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHH
AAAAKAFKAG
HHHHHHHCCC
>Mature Secondary Structure 
SRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITRAEGAYMWDADGQRYIDYIGSWG
CCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCCEEECCCCHHHHHHHCCCC
PMIVGHAHPDVVRAVQETATQSFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATM
CEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
SALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTFADTTQNAPSSAGVPADVTRH
HHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCC
TMVLEYNNVTQLEEAFARHKGEIAAVIVEPVAGNMNLVRASEAFLTAMRNLCSEHGSVLI
EEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCEEE
FDEVMTGFRVALGGAQAHYGIKPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQA
EHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEC
GTLSGNPLAVAAGLTTLRLIQAPGFYDRLATQTRKLADGLTKAAREAGVPFCADAIGGMF
CCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCE
GIYFTNEVPGSFAEVTKADVGRFNRFFHAMLAEGVYLAPSAFEAGFVSAKHDDAIIEATV
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHH
AAAAKAFKAG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA