Click here to switch to the map view.

The map label for this gene is rpoO [H]

Identifier: 94309542

GI number: 94309542

Start: 644668

End: 645297

Strand: Reverse

Name: rpoO [H]

Synonym: Rmet_0597

Alternate gene names: 94309542

Gene position: 645297-644668 (Counterclockwise)

Preceding gene: 94309545

Following gene: 94309541

Centisome position: 16.43

GC content: 68.89

Gene sequence:

>630_bases
TTGGACGCCGCCAACACGCGCCGCCGCTTCGACGACCTGGTGCTGCCGCATCTGGACGCCGCCTACAACCTCGCCCGCTG
GTTATGCGGCAATCCGGGCGATGCCGATGACGTGGTGCAGGAAGCATTCCTGCGCGCGTTCCGGCTGTTCGGCAGCTTCC
GCGGCGACCAGCCCCGGCCCTGGCTGCTGGCCATCGTGCGCAACACGTGGTTCACCACGTGGCAGAAGCGCAAGGCGGCG
GCCGAGGTGGAGTACGACGAAGCCGCGCCGCCGGGTGAACTACCCGACTGGCATGGCGGGCCCGGCGACCCGGAGCAATG
GCTGGTGCGCGACGAGGATGTGCGACTGGTGCATGCCGCGCTGGAGCGACTGCCGGTCGTGTTTCGGGAGGTACTGGTGC
TGCGCGAACTGGAGGAACTGCATTACCGCGACATCGCACGCATCGCCGATATTCCGGTCGGCACCGTGATGTCGCGGCTG
GCACGTGGCAGGCGAATGCTGGCCGCCGCCGTCGTGGCCGTGCGCGACGAAGGCGCGGTACGGCTGGCACATCCCGACGC
CCCCAACACCGCCAACGCCCCGGCCGATATCGTTCAACCCGCAGGAGGCACCCGCCATGGACTGCAATGA

Upstream 100 bases:

>100_bases
GGACAAGCGCCGGGAATAGAATCGGCCCAGCGCCGGTATAACGGGGGAGACGCACTCATGCCGCTATGCCATTTCGACCA
GCCGACACGCAAGGACTGAC

Downstream 100 bases:

>100_bases
CGCCCGCAACCTGCTGCAGGCCTGCGCCGATGGCGAAGTCGGCGCGGCCGACAGCGTGCGTCTGGAACGCCACCTCGAGG
ATTGCGAGTCGTGCGTCGAC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MDAANTRRRFDDLVLPHLDAAYNLARWLCGNPGDADDVVQEAFLRAFRLFGSFRGDQPRPWLLAIVRNTWFTTWQKRKAA
AEVEYDEAAPPGELPDWHGGPGDPEQWLVRDEDVRLVHAALERLPVVFREVLVLRELEELHYRDIARIADIPVGTVMSRL
ARGRRMLAAAVVAVRDEGAVRLAHPDAPNTANAPADIVQPAGGTRHGLQ

Sequences:

>Translated_209_residues
MDAANTRRRFDDLVLPHLDAAYNLARWLCGNPGDADDVVQEAFLRAFRLFGSFRGDQPRPWLLAIVRNTWFTTWQKRKAA
AEVEYDEAAPPGELPDWHGGPGDPEQWLVRDEDVRLVHAALERLPVVFREVLVLRELEELHYRDIARIADIPVGTVMSRL
ARGRRMLAAAVVAVRDEGAVRLAHPDAPNTANAPADIVQPAGGTRHGLQ
>Mature_209_residues
MDAANTRRRFDDLVLPHLDAAYNLARWLCGNPGDADDVVQEAFLRAFRLFGSFRGDQPRPWLLAIVRNTWFTTWQKRKAA
AEVEYDEAAPPGELPDWHGGPGDPEQWLVRDEDVRLVHAALERLPVVFREVLVLRELEELHYRDIARIADIPVGTVMSRL
ARGRRMLAAAVVAVRDEGAVRLAHPDAPNTANAPADIVQPAGGTRHGLQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=139, Percent_Identity=38.8489208633094, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23392; Mature: 23392

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAANTRRRFDDLVLPHLDAAYNLARWLCGNPGDADDVVQEAFLRAFRLFGSFRGDQPRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
WLLAIVRNTWFTTWQKRKAAAEVEYDEAAPPGELPDWHGGPGDPEQWLVRDEDVRLVHAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHH
LERLPVVFREVLVLRELEELHYRDIARIADIPVGTVMSRLARGRRMLAAAVVAVRDEGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
RLAHPDAPNTANAPADIVQPAGGTRHGLQ
EEECCCCCCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDAANTRRRFDDLVLPHLDAAYNLARWLCGNPGDADDVVQEAFLRAFRLFGSFRGDQPRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
WLLAIVRNTWFTTWQKRKAAAEVEYDEAAPPGELPDWHGGPGDPEQWLVRDEDVRLVHAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHH
LERLPVVFREVLVLRELEELHYRDIARIADIPVGTVMSRLARGRRMLAAAVVAVRDEGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
RLAHPDAPNTANAPADIVQPAGGTRHGLQ
EEECCCCCCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]