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The map label for this gene is ogt1 [H]

Identifier: 94309465

GI number: 94309465

Start: 555999

End: 556478

Strand: Reverse

Name: ogt1 [H]

Synonym: Rmet_0520

Alternate gene names: 94309465

Gene position: 556478-555999 (Counterclockwise)

Preceding gene: 94309466

Following gene: 94309464

Centisome position: 14.17

GC content: 65.0

Gene sequence:

>480_bases
ATGCAACATCACTTCGATGCCGTCCTGCCCGCGCCATTTGGCAAGGTCGGCGTACGTGTCGATGGCACGCAGGTTCACGA
AATCGTCTATCTGCCCGACAGCTTTGCCGAGGTCCCGCCAACCAGCGCGTTGACAAAGCGCGTGGCGGAACAGCTCGACG
CCTACTACGCCAATGCAGACACCGTGTTCGACCTGCCGCTGGCACGGCGTGGCACGGACTTCCAGCGCAAAGTCTGGGCG
GCCATCAGCGCGGTGCCGCGTGGCGGCCTGACGACCTACGGCACCATCGCGAAATCGCTGCAGAGCATGCCGCGCGCCGT
GGGCCAGGCCTGCGGACAGAACTGGTTTCCGATCGTGGTGCCGTGCCATCGCGTGGTGGCGGCCAACGGACTCGGCGGCT
TCGCGCATCATGGCGAGGAAGGATTCCATCTGGGCGTGAAGCGCTGGCTGCTGCGCCATGAAGGCGCGATGCTGCTATGA

Upstream 100 bases:

>100_bases
CTGTCTGCCCCCGCCGAGCGGGCGGGTAGTCCCATTGCATTCGATATGCATCTGGAATCAGCATAAAATCGCTCATCGCC
ACGTTATCCCGCCATCCACC

Downstream 100 bases:

>100_bases
CGGCGGCACTCGATGGCGACCTGTCGCTGGTCGACCGGTTCTGCGATGCGTTGTGGCTCGAAGATGGCCTGGCACGCAAT
ACGATCGACGCCTACCGGCG

Product: Methylated-DNA (protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MQHHFDAVLPAPFGKVGVRVDGTQVHEIVYLPDSFAEVPPTSALTKRVAEQLDAYYANADTVFDLPLARRGTDFQRKVWA
AISAVPRGGLTTYGTIAKSLQSMPRAVGQACGQNWFPIVVPCHRVVAANGLGGFAHHGEEGFHLGVKRWLLRHEGAMLL

Sequences:

>Translated_159_residues
MQHHFDAVLPAPFGKVGVRVDGTQVHEIVYLPDSFAEVPPTSALTKRVAEQLDAYYANADTVFDLPLARRGTDFQRKVWA
AISAVPRGGLTTYGTIAKSLQSMPRAVGQACGQNWFPIVVPCHRVVAANGLGGFAHHGEEGFHLGVKRWLLRHEGAMLL
>Mature_159_residues
MQHHFDAVLPAPFGKVGVRVDGTQVHEIVYLPDSFAEVPPTSALTKRVAEQLDAYYANADTVFDLPLARRGTDFQRKVWA
AISAVPRGGLTTYGTIAKSLQSMPRAVGQACGQNWFPIVVPCHRVVAANGLGGFAHHGEEGFHLGVKRWLLRHEGAMLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=141, Percent_Identity=38.2978723404255, Blast_Score=83, Evalue=7e-18,
Organism=Escherichia coli, GI1788542, Length=139, Percent_Identity=33.0935251798561, Blast_Score=67, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320001, Length=61, Percent_Identity=50.8196721311475, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17327; Mature: 17327

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHHFDAVLPAPFGKVGVRVDGTQVHEIVYLPDSFAEVPPTSALTKRVAEQLDAYYANAD
CCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TVFDLPLARRGTDFQRKVWAAISAVPRGGLTTYGTIAKSLQSMPRAVGQACGQNWFPIVV
EEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
PCHRVVAANGLGGFAHHGEEGFHLGVKRWLLRHEGAMLL
HHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQHHFDAVLPAPFGKVGVRVDGTQVHEIVYLPDSFAEVPPTSALTKRVAEQLDAYYANAD
CCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TVFDLPLARRGTDFQRKVWAAISAVPRGGLTTYGTIAKSLQSMPRAVGQACGQNWFPIVV
EEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
PCHRVVAANGLGGFAHHGEEGFHLGVKRWLLRHEGAMLL
HHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]