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The map label for this gene is pgk2 [H]

Identifier: 94309446

GI number: 94309446

Start: 530997

End: 532253

Strand: Direct

Name: pgk2 [H]

Synonym: Rmet_0501

Alternate gene names: 94309446

Gene position: 530997-532253 (Clockwise)

Preceding gene: 94309443

Following gene: 94309447

Centisome position: 13.52

GC content: 65.16

Gene sequence:

>1257_bases
ATGCCGGTTTTCCCGATTTCCACGGGCGGCGCATGTCGCCCACGTCCCGCTCTTATCGCCATGACCTCTGTCCTGCGTCT
TACCGATCTCATTTCCCAAGGCAAAATCTCCGGCAAACGTGTGTTCATCCGTGCCGACCTGAACGTGCCGCAAGACGACG
CAGGCAATATCACCGAAGACACCCGCATCCGCGCGTCGGTGCCGGCCATTCAGGCCTGCCTGGAAGCCGGCGCGACCGTC
ATGGTGACGTCGCACCTGGGCCGTCCGACCGAGGGCGAGTTCAAGCCGGAGGATTCGCTGGCACCGATTGCCACGCGCCT
GTCCGAACTGCTCGGCAAGCCGGTGAAACTGGTCCAGAACTGGGTCGACGGTGTCGAGGTGGCGCCTGGCCAGATCGTGC
TCCTGGAAAACTGCCGCGTGAACAAGGGCGAGAAGAAGAACAGCGACGAGCTGGCCCAGAAGATGGCCAAGCTCTGTGAC
GTCTACGTGAACGACGCGTTCGGCACCGCCCACCGCGCCGAGGCCACCACTCACGGTATCGCCAAGTTTGCCCCGATCGC
CTGCGCCGGCCCGCTGCTGGCCGCCGAGATCGACGCGCTGGGCCGCGCGCTGGGCCAGCCGGCGCGTCCGCTGGTGGCCA
TCGTGGCCGGCTCGAAGGTTTCGACCAAGCTCACCATCCTGAAGGCACTGGCCGACAAGGTGGACAACCTGATCGTCGGC
GGCGGCATCGCCAACACGTTCATGCTGGCCGCTGGCCTGAAGATCGGCAAGTCGCTCGCCGAGGCCGACCTCGTGGGTGA
CGCCAAGACGATCATCGACATCATGGCCGCGCGCGGCGCATCGGTGCCGATTCCCGTCGACGTGGTCTGCGCCAAGGAGT
TCAGCGCCACGGCTGCGGCGACGGTCAAGGACGTCAAGGACGTTACCGACGACGACATGATCCTCGACATCGGCCCGAAG
ACCGCCGCGATGCTGGCCGACCAGCTCAAGGCTGCCGGCACGATCGTCTGGAACGGTCCGGTCGGCGTGTTCGAATTCGA
TCAGTTCGGCAACGGTACCAAGGTGCTGGCCGAAGCCATCGCCACGTCGAAGGCATTCTCGATTGCAGGTGGCGGCGACA
CGCTGGCCGCCATCGCCAAGTACGGCATCGCCGATCGCGTCGGGTATATCTCGACCGGTGGCGGCGCCTTCCTGGAATTC
CTGGAAGGCAAGAAGCTGCCCGCATTCGAAGTGCTGGAACAGCGCGCCGCAGGCTGA

Upstream 100 bases:

>100_bases
CCCCCCCTTCGGTAGGGGATGTTCCGGGCTGGGCATGGCATGTGACGGATATGCCGCGAAACGGGCAGATCGCGTCGTAC
GGTAGTAACGATCAGGTAAA

Downstream 100 bases:

>100_bases
TGACCCGACAGGCCGGCGCGGGACACCCCCGTCCGGCCAGTTTCCAGAAAAACCAAGCGCCCCCCACCAAGGAAGCTGCC
CGCATGACACGTTCCACCAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPVFPISTGGACRPRPALIAMTSVLRLTDLISQGKISGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIQACLEAGATV
MVTSHLGRPTEGEFKPEDSLAPIATRLSELLGKPVKLVQNWVDGVEVAPGQIVLLENCRVNKGEKKNSDELAQKMAKLCD
VYVNDAFGTAHRAEATTHGIAKFAPIACAGPLLAAEIDALGRALGQPARPLVAIVAGSKVSTKLTILKALADKVDNLIVG
GGIANTFMLAAGLKIGKSLAEADLVGDAKTIIDIMAARGASVPIPVDVVCAKEFSATAAATVKDVKDVTDDDMILDIGPK
TAAMLADQLKAAGTIVWNGPVGVFEFDQFGNGTKVLAEAIATSKAFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEF
LEGKKLPAFEVLEQRAAG

Sequences:

>Translated_418_residues
MPVFPISTGGACRPRPALIAMTSVLRLTDLISQGKISGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIQACLEAGATV
MVTSHLGRPTEGEFKPEDSLAPIATRLSELLGKPVKLVQNWVDGVEVAPGQIVLLENCRVNKGEKKNSDELAQKMAKLCD
VYVNDAFGTAHRAEATTHGIAKFAPIACAGPLLAAEIDALGRALGQPARPLVAIVAGSKVSTKLTILKALADKVDNLIVG
GGIANTFMLAAGLKIGKSLAEADLVGDAKTIIDIMAARGASVPIPVDVVCAKEFSATAAATVKDVKDVTDDDMILDIGPK
TAAMLADQLKAAGTIVWNGPVGVFEFDQFGNGTKVLAEAIATSKAFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEF
LEGKKLPAFEVLEQRAAG
>Mature_417_residues
PVFPISTGGACRPRPALIAMTSVLRLTDLISQGKISGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIQACLEAGATVM
VTSHLGRPTEGEFKPEDSLAPIATRLSELLGKPVKLVQNWVDGVEVAPGQIVLLENCRVNKGEKKNSDELAQKMAKLCDV
YVNDAFGTAHRAEATTHGIAKFAPIACAGPLLAAEIDALGRALGQPARPLVAIVAGSKVSTKLTILKALADKVDNLIVGG
GIANTFMLAAGLKIGKSLAEADLVGDAKTIIDIMAARGASVPIPVDVVCAKEFSATAAATVKDVKDVTDDDMILDIGPKT
AAMLADQLKAAGTIVWNGPVGVFEFDQFGNGTKVLAEAIATSKAFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEFL
EGKKLPAFEVLEQRAAG

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=41.747572815534, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=40.0485436893204, Blast_Score=255, Evalue=6e-68,
Organism=Escherichia coli, GI1789294, Length=394, Percent_Identity=62.4365482233503, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17508823, Length=420, Percent_Identity=42.3809523809524, Blast_Score=286, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6319857, Length=428, Percent_Identity=39.9532710280374, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17136394, Length=405, Percent_Identity=40, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24581203, Length=403, Percent_Identity=36.9727047146402, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 43338; Mature: 43206

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVFPISTGGACRPRPALIAMTSVLRLTDLISQGKISGKRVFIRADLNVPQDDAGNITED
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC
TRIRASVPAIQACLEAGATVMVTSHLGRPTEGEFKPEDSLAPIATRLSELLGKPVKLVQN
CEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
WVDGVEVAPGQIVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGI
HHCCCEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AKFAPIACAGPLLAAEIDALGRALGQPARPLVAIVAGSKVSTKLTILKALADKVDNLIVG
HHHCCHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEC
GGIANTFMLAAGLKIGKSLAEADLVGDAKTIIDIMAARGASVPIPVDVVCAKEFSATAAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCHHCHHHHH
TVKDVKDVTDDDMILDIGPKTAAMLADQLKAAGTIVWNGPVGVFEFDQFGNGTKVLAEAI
HHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCEEEECCCCCEEEHHCCCCHHHHHHHHH
ATSKAFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEFLEGKKLPAFEVLEQRAAG
HHHHHEEECCCCHHHHHHHHHCHHHCCCEEECCHHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
PVFPISTGGACRPRPALIAMTSVLRLTDLISQGKISGKRVFIRADLNVPQDDAGNITED
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC
TRIRASVPAIQACLEAGATVMVTSHLGRPTEGEFKPEDSLAPIATRLSELLGKPVKLVQN
CEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
WVDGVEVAPGQIVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGI
HHCCCEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AKFAPIACAGPLLAAEIDALGRALGQPARPLVAIVAGSKVSTKLTILKALADKVDNLIVG
HHHCCHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEC
GGIANTFMLAAGLKIGKSLAEADLVGDAKTIIDIMAARGASVPIPVDVVCAKEFSATAAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCHHCHHHHH
TVKDVKDVTDDDMILDIGPKTAAMLADQLKAAGTIVWNGPVGVFEFDQFGNGTKVLAEAI
HHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCEEEECCCCCEEEHHCCCCHHHHHHHHH
ATSKAFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEFLEGKKLPAFEVLEQRAAG
HHHHHEEECCCCHHHHHHHHHCHHHCCCEEECCHHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]