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The map label for this gene is pykA1 [H]

Identifier: 94309447

GI number: 94309447

Start: 532337

End: 533776

Strand: Direct

Name: pykA1 [H]

Synonym: Rmet_0502

Alternate gene names: 94309447

Gene position: 532337-533776 (Clockwise)

Preceding gene: 94309446

Following gene: 94309448

Centisome position: 13.55

GC content: 65.83

Gene sequence:

>1440_bases
ATGACACGTTCCACCAAGATCGTCGCCACGATTGGCCCCGCATCCAGCACGCTGGAAGTGTTGACCCGCATGATCGGAGC
AGGGGTTGACGTGGTGCGCCTGAACTTCTCGCATGGCACCGCGCAGGACCATATCGATCGCGCCGCCCTGGTGCGTGAGG
CGGCGGCGGCCTGCGGGCGCGAGGTGGCCATCATGGCCGACCTGCAGGGCCCCAAGATCCGCGTGGGCAAGTTCGAGAAC
GGCAAGATCACGCTCAAGCCGGGCGACGCGTTCATCCTCGATTCGGCCTGCCAGATGGGCAATCAGGAGCGCGTGGGCCT
GGATTACAAGGATCTGCCGCGCGATGTGGGCCCGGGCGACCTGCTCCTGCTCAACGACGGCCTGATCGTGCTGGTGGTGG
ACCGCGTGCTCGGCAACGAGATCTTCACCACGGTGCGTATCGGTGGCGAGCTGTCCAACAACAAGGGCATCAATCGTCAG
GGTGGCGGCCTGTCGGCGCCGGCGCTGACCGCCAAGGACATGGAGGACATCAAGACCGCCATGGCGCTGGGCGCGGACTA
CGTGGCCGTGAGCTTCCCGAAGAACGCCACTGACATGGAAATGGCCCGCCAGCTCACCAACGTGGCAGGCCAGCCGCACA
ACCACAAGGCGCGCATGATCGCGAAGATCGAGCGGGCCGAGGCGATTCGTCCGGGCGTGCTCGAGGAAATCCTGCTGGCG
TCGGACGGCATCATGGTGGCGCGCGGCGATCTGGCCGTCGAAGTTGGCAACGCCGCCGTGCCCGCGCTGCAGAAGCGCAT
GATCAAGCTGGCCCGCGAGGCCAACAAGCTGACGATCACCGCCACCCAGATGATGGAGAGCATGATCGTCAACCCGGTGC
CGACGCGGGCCGAAGTGTCCGACGTGGCCAACGCCGTGCTCGATGGCACCGACGCCGTGATGACGTCGGCCGAGACCGCT
GCCGGCCGCTATCCGGTGGAAACGGTCGAAGCCATGGCCGCGATCTGTCTGGAAGCCGAGAAGTCGGAGGTGGTGCAGCT
CGACACCGACTTCCTGAACCAGACCTTCGCGCGGATCGACCAGTCGATCGCGATGGGTGCGCTGTTCACCGCCTATCATC
TAGGTGTCAAGGCGATCGCGGCGCTGACCGATTCCGGCGCCACGGCGCTGTGGATGAGCCGTCACCGCATTCATGTGCCG
ATCTACGCGATGACGCCGAACCTGGCGTCGCAACGCAAGATGCAGCTCTATCGCAATGTGGTGCCGCTGCCGCTGCAGTC
GAGCGCGGACCGCGACGTGGCGCTTGAGCAGGCCGAGGAACTGCTGCTGTCCCAGGGCGTGGTGCAGCGCGGCGATTTCA
TCGTGCTGACGATCGGCGAGCCGATGGGCCAGCCCGGCGGCACGAATACGCTCAAGATCGTGAGGGTGGGCACCCGGTAG

Upstream 100 bases:

>100_bases
AGCGCGCCGCAGGCTGATGACCCGACAGGCCGGCGCGGGACACCCCCGTCCGGCCAGTTTCCAGAAAAACCAAGCGCCCC
CCACCAAGGAAGCTGCCCGC

Downstream 100 bases:

>100_bases
GACGCGCGCCCCATGTGGCGCGCCAGACAGACATGACCGCGCCCGATGCGCGAGCCTGTTTGGCGCAGCAACGATAAGAA
TTCAGAAATACCCCAAGCTA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTRSTKIVATIGPASSTLEVLTRMIGAGVDVVRLNFSHGTAQDHIDRAALVREAAAACGREVAIMADLQGPKIRVGKFEN
GKITLKPGDAFILDSACQMGNQERVGLDYKDLPRDVGPGDLLLLNDGLIVLVVDRVLGNEIFTTVRIGGELSNNKGINRQ
GGGLSAPALTAKDMEDIKTAMALGADYVAVSFPKNATDMEMARQLTNVAGQPHNHKARMIAKIERAEAIRPGVLEEILLA
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMTSAETA
AGRYPVETVEAMAAICLEAEKSEVVQLDTDFLNQTFARIDQSIAMGALFTAYHLGVKAIAALTDSGATALWMSRHRIHVP
IYAMTPNLASQRKMQLYRNVVPLPLQSSADRDVALEQAEELLLSQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGTR

Sequences:

>Translated_479_residues
MTRSTKIVATIGPASSTLEVLTRMIGAGVDVVRLNFSHGTAQDHIDRAALVREAAAACGREVAIMADLQGPKIRVGKFEN
GKITLKPGDAFILDSACQMGNQERVGLDYKDLPRDVGPGDLLLLNDGLIVLVVDRVLGNEIFTTVRIGGELSNNKGINRQ
GGGLSAPALTAKDMEDIKTAMALGADYVAVSFPKNATDMEMARQLTNVAGQPHNHKARMIAKIERAEAIRPGVLEEILLA
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMTSAETA
AGRYPVETVEAMAAICLEAEKSEVVQLDTDFLNQTFARIDQSIAMGALFTAYHLGVKAIAALTDSGATALWMSRHRIHVP
IYAMTPNLASQRKMQLYRNVVPLPLQSSADRDVALEQAEELLLSQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGTR
>Mature_478_residues
TRSTKIVATIGPASSTLEVLTRMIGAGVDVVRLNFSHGTAQDHIDRAALVREAAAACGREVAIMADLQGPKIRVGKFENG
KITLKPGDAFILDSACQMGNQERVGLDYKDLPRDVGPGDLLLLNDGLIVLVVDRVLGNEIFTTVRIGGELSNNKGINRQG
GGLSAPALTAKDMEDIKTAMALGADYVAVSFPKNATDMEMARQLTNVAGQPHNHKARMIAKIERAEAIRPGVLEEILLAS
DGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMTSAETAA
GRYPVETVEAMAAICLEAEKSEVVQLDTDFLNQTFARIDQSIAMGALFTAYHLGVKAIAALTDSGATALWMSRHRIHVPI
YAMTPNLASQRKMQLYRNVVPLPLQSSADRDVALEQAEELLLSQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGTR

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=495, Percent_Identity=37.979797979798, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI33286420, Length=495, Percent_Identity=37.979797979798, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=38.1818181818182, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI10835121, Length=502, Percent_Identity=36.8525896414343, Blast_Score=271, Evalue=9e-73,
Organism=Homo sapiens, GI32967597, Length=502, Percent_Identity=36.8525896414343, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=37.6712328767123, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=37.6712328767123, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=35, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=35, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI310128738, Length=219, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=52.092050209205, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1787965, Length=476, Percent_Identity=38.4453781512605, Blast_Score=297, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=37.5770020533881, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=37.0672097759674, Blast_Score=254, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=37.0672097759674, Blast_Score=254, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=37.0672097759674, Blast_Score=254, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=37.0672097759674, Blast_Score=254, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6319279, Length=495, Percent_Identity=33.3333333333333, Blast_Score=239, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6324923, Length=495, Percent_Identity=35.3535353535354, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.8542510121458, Blast_Score=271, Evalue=5e-73,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.8542510121458, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=37.3170731707317, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=31.8367346938775, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24646914, Length=275, Percent_Identity=36, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51181; Mature: 51050

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSTKIVATIGPASSTLEVLTRMIGAGVDVVRLNFSHGTAQDHIDRAALVREAAAACGR
CCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EVAIMADLQGPKIRVGKFENGKITLKPGDAFILDSACQMGNQERVGLDYKDLPRDVGPGD
CEEEEEECCCCEEEEEEECCCEEEECCCCEEEEEHHHHCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLVVDRVLGNEIFTTVRIGGELSNNKGINRQGGGLSAPALTAKDMEDIKTA
EEEEECCEEEEEEHHHHCCHHEEEEEECCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MALGADYVAVSFPKNATDMEMARQLTNVAGQPHNHKARMIAKIERAEAIRPGVLEEILLA
HHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVS
CCCEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHH
DVANAVLDGTDAVMTSAETAAGRYPVETVEAMAAICLEAEKSEVVQLDTDFLNQTFARID
HHHHHHHCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHH
QSIAMGALFTAYHLGVKAIAALTDSGATALWMSRHRIHVPIYAMTPNLASQRKMQLYRNV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCC
VPLPLQSSADRDVALEQAEELLLSQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGTR
CCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TRSTKIVATIGPASSTLEVLTRMIGAGVDVVRLNFSHGTAQDHIDRAALVREAAAACGR
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EVAIMADLQGPKIRVGKFENGKITLKPGDAFILDSACQMGNQERVGLDYKDLPRDVGPGD
CEEEEEECCCCEEEEEEECCCEEEECCCCEEEEEHHHHCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLVVDRVLGNEIFTTVRIGGELSNNKGINRQGGGLSAPALTAKDMEDIKTA
EEEEECCEEEEEEHHHHCCHHEEEEEECCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MALGADYVAVSFPKNATDMEMARQLTNVAGQPHNHKARMIAKIERAEAIRPGVLEEILLA
HHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
SDGIMVARGDLAVEVGNAAVPALQKRMIKLAREANKLTITATQMMESMIVNPVPTRAEVS
CCCEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHH
DVANAVLDGTDAVMTSAETAAGRYPVETVEAMAAICLEAEKSEVVQLDTDFLNQTFARID
HHHHHHHCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHH
QSIAMGALFTAYHLGVKAIAALTDSGATALWMSRHRIHVPIYAMTPNLASQRKMQLYRNV
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCC
VPLPLQSSADRDVALEQAEELLLSQGVVQRGDFIVLTIGEPMGQPGGTNTLKIVRVGTR
CCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]