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The map label for this gene is ostA

Identifier: 94309381

GI number: 94309381

Start: 460771

End: 463245

Strand: Direct

Name: ostA

Synonym: Rmet_0436

Alternate gene names: 94309381

Gene position: 460771-463245 (Clockwise)

Preceding gene: 94309365

Following gene: 94309382

Centisome position: 11.73

GC content: 63.07

Gene sequence:

>2475_bases
ATGACCGAACAACGACGATCACCGCATCATCCGGCCACCCGACCGCCAGCGCCTCCGGGCACGTCCCGGCGCGTCCGCCT
GCCCGCCAGCGCGCTGCGGCCGCTCGTGCTCGCCATGGCCGGGCTGACGGTGAGTGCCCATGCGCAGTACGGCGCGACGT
CCGCCATTCCGAACATCGACCTTGTCGAGCCGGTGGTCACTCAGACCCCGGAGGCGCCGCCGCCGCCGGCTGAAGGCGGC
GATCTCGTGCCGCGCCTGACCGAGCCCGCCACGCGGACCGCCCCCAGTGGCAACACGCTAAACCTCTCGCCGTCATCTAC
GCCCTCGAATCCGAACGCCCCGGCCTATGTGTCGGGCGATCGCGTAACGGGCTACAGCGAGAAGGGCGTGGAGATGGAAG
GGCACGCCGAACTGCGCCGCGACGGTGGCGTGATCAAGGGCGACAGGCTCACCTACGACCAGGACACCGACGAAGCACAT
GCCACTGGCAACGTGCGGCTTTCCAAGAGCGGCACGCTGGCCGTGGGCCCGGAAGCACGGATGCGCGTGCAGGCCAATGA
AGGCTACATGCTGTCCCCGGACTACTACTTCCAGCAGACTGGCGGCTCCGGCAGCGCCGAGCGCGTCGATTTCCTGGATC
CGGACCGCAGCACGCTGAAGAAGGCGACCTACACGACCTGTTCGCCCGATAACGCGGACTGGTACTTCAGCGCCAGAAAG
CTCGACCTGGACAGCGATCGTCAGGTCGGCACGGCCTATGGCGGTGTGCTTAATTTCTTTGGCGTGCCGATTGCCGGCGC
GCCGGCGTTCAGTTTTCCGCTCAATGGCGAGCGTCGTAGCGGCGTGCTGCCGCCGTTGTTCGGCTATGGGTCGAACTCGG
GTGCCGACCTGACGGTGCCCTACTACTTCAACCTGGCGCCAAACCGCGATCTGACGATCTACCCGCGTATCCTGACGTCG
CGCGGCGTGCAGCTTGGCGAGGATTTCCGGTATGTGGGCGACGGCTACAGCGGACGTATCCGCGGCGAGTTCCTGCCCGA
CGACAAGAAGGCGGGCCGCAACCGTTGGGCCTACTCGATCCAGCACTACCAGAGCATCATTCCTGGTATGACGGCCTATG
TGAACGTCAGCAAGGTTTCGGATGACAAGTATCCCGACGACCTGACGCGTTCCGTATCGCAGTCGACTCTGCGTCAGTAC
ACGCAGGAAGGTGGCGTGATCTATGCGTGGCAGGACTGGGTGTTCATGGCACGGGTGCAGAAGTTCCAGACGCTGTTGCC
CAGCGAGCCGTCCTACGAGCGCGAGCCGCAGTTGAACGCGAAGTACAACCGCTATGATTTCCACGGTTTCGATATTTCGC
TCGAAACTGACTACACGCGGTTCCGCATCCCGCTGACCAGTACCGGCTTCCAGCAGCCCGAGGGCAATCGCGCATTCATC
CAGCCGACGATCAGTTACCCGATCATCCACCCGGGCTGGTACGTGACGCCGAAGTTCATCTTCAACGCGGCGCAGTACAA
CATGGATGCCGGCACCAACACCACTGGCGCGTCGAATACGCTCAATCGCGCCATTCCGACCGTGAGCCTCGATTCGGGCA
TGACGTTCGAGCGTGACGCGCCGGGTGTGAGCAAGCTGTTTGGCGTGAAGTACACCCAGACACTCGAGCCGCGCCTGTTC
TACGTCTACACGCCGTTCTACGACCAGAGTCAGTTCCCGCTGTTCGATACAGTGCAGTCGGACTTCGGCTACGGCCAGAT
CTTTACCGAGAACCCGTTTACCGGTAACGACCGCATCGCCGACAATAACAAGCTGACGCTGGGCCTGACCACGCGCCTGA
TCGAATCGGAGACCGGTGTCGAACGTTTCCGTGGCACGATTGCGCAGCGCGTCGACTTCACGGGCCAGCGTGTCCAGCTC
AACGGCACGCTGCCGGATGCCAAGCCGAGCTACTCGGACCTGCTGGCAGCCACCACGATCCAGCTGTTCCGTGGCTACTA
TCTCGATGCCGGCATCCAGTGGAATCCGGACCAGGACAAGGTCAACTACTCGAACGTGGCACTCGCGTACCGCCCGGAAT
CGCGCAAGCTCATCAATTTTGGCTATCGCTACCGTCGCCCGACCTCGGTGACAGACAATACCGCGATCGACCAGATCGAA
ATGTCGGGACAGTGGCCGATCACGCAACGCACTTACGGTATCGGCCGCGTGGCGTTCGACAAGTCGGCCAACCAGCTCGT
GGATGCGCTGGCCGGTTTCGAGTATGCCGCCGACTGCTGGGTCGGCCGGTTCGTGTACCAGCGTTTCCGCAATACCTCGA
ACGGTTACACCGGGCGGGTTTTCTTCCAGGTGGAGTTCCGTGGGCTGTCGAAGATCGGCTCCAACCCGCTGGACATGTTG
CGCCTGAACGTGCCGGGCTACGAGCCCGTGACGGCACGGCCGGTGCCGACAACCCCGTACGATCACTATGAATGA

Upstream 100 bases:

>100_bases
CCCCGCAGCCCGCTCTGGCAATGGGCTGGCGTTGAAGCAGGGGCAAACTTTGCAGCCCCTTCGAAGCGCCGGCCCGCCGA
GCTGACCGACCACCGTTTGC

Downstream 100 bases:

>100_bases
CGGACCAAGATGAAACGTCAAGCCTTCTCCCTGTTGTCCCGCCTGAACCCCTGGCAGCAGCTGCTGCTGTCGGCCGTGCT
CGTGACGCTGGCCGCGCCGG

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein

Number of amino acids: Translated: 824; Mature: 823

Protein sequence:

>824_residues
MTEQRRSPHHPATRPPAPPGTSRRVRLPASALRPLVLAMAGLTVSAHAQYGATSAIPNIDLVEPVVTQTPEAPPPPAEGG
DLVPRLTEPATRTAPSGNTLNLSPSSTPSNPNAPAYVSGDRVTGYSEKGVEMEGHAELRRDGGVIKGDRLTYDQDTDEAH
ATGNVRLSKSGTLAVGPEARMRVQANEGYMLSPDYYFQQTGGSGSAERVDFLDPDRSTLKKATYTTCSPDNADWYFSARK
LDLDSDRQVGTAYGGVLNFFGVPIAGAPAFSFPLNGERRSGVLPPLFGYGSNSGADLTVPYYFNLAPNRDLTIYPRILTS
RGVQLGEDFRYVGDGYSGRIRGEFLPDDKKAGRNRWAYSIQHYQSIIPGMTAYVNVSKVSDDKYPDDLTRSVSQSTLRQY
TQEGGVIYAWQDWVFMARVQKFQTLLPSEPSYEREPQLNAKYNRYDFHGFDISLETDYTRFRIPLTSTGFQQPEGNRAFI
QPTISYPIIHPGWYVTPKFIFNAAQYNMDAGTNTTGASNTLNRAIPTVSLDSGMTFERDAPGVSKLFGVKYTQTLEPRLF
YVYTPFYDQSQFPLFDTVQSDFGYGQIFTENPFTGNDRIADNNKLTLGLTTRLIESETGVERFRGTIAQRVDFTGQRVQL
NGTLPDAKPSYSDLLAATTIQLFRGYYLDAGIQWNPDQDKVNYSNVALAYRPESRKLINFGYRYRRPTSVTDNTAIDQIE
MSGQWPITQRTYGIGRVAFDKSANQLVDALAGFEYAADCWVGRFVYQRFRNTSNGYTGRVFFQVEFRGLSKIGSNPLDML
RLNVPGYEPVTARPVPTTPYDHYE

Sequences:

>Translated_824_residues
MTEQRRSPHHPATRPPAPPGTSRRVRLPASALRPLVLAMAGLTVSAHAQYGATSAIPNIDLVEPVVTQTPEAPPPPAEGG
DLVPRLTEPATRTAPSGNTLNLSPSSTPSNPNAPAYVSGDRVTGYSEKGVEMEGHAELRRDGGVIKGDRLTYDQDTDEAH
ATGNVRLSKSGTLAVGPEARMRVQANEGYMLSPDYYFQQTGGSGSAERVDFLDPDRSTLKKATYTTCSPDNADWYFSARK
LDLDSDRQVGTAYGGVLNFFGVPIAGAPAFSFPLNGERRSGVLPPLFGYGSNSGADLTVPYYFNLAPNRDLTIYPRILTS
RGVQLGEDFRYVGDGYSGRIRGEFLPDDKKAGRNRWAYSIQHYQSIIPGMTAYVNVSKVSDDKYPDDLTRSVSQSTLRQY
TQEGGVIYAWQDWVFMARVQKFQTLLPSEPSYEREPQLNAKYNRYDFHGFDISLETDYTRFRIPLTSTGFQQPEGNRAFI
QPTISYPIIHPGWYVTPKFIFNAAQYNMDAGTNTTGASNTLNRAIPTVSLDSGMTFERDAPGVSKLFGVKYTQTLEPRLF
YVYTPFYDQSQFPLFDTVQSDFGYGQIFTENPFTGNDRIADNNKLTLGLTTRLIESETGVERFRGTIAQRVDFTGQRVQL
NGTLPDAKPSYSDLLAATTIQLFRGYYLDAGIQWNPDQDKVNYSNVALAYRPESRKLINFGYRYRRPTSVTDNTAIDQIE
MSGQWPITQRTYGIGRVAFDKSANQLVDALAGFEYAADCWVGRFVYQRFRNTSNGYTGRVFFQVEFRGLSKIGSNPLDML
RLNVPGYEPVTARPVPTTPYDHYE
>Mature_823_residues
TEQRRSPHHPATRPPAPPGTSRRVRLPASALRPLVLAMAGLTVSAHAQYGATSAIPNIDLVEPVVTQTPEAPPPPAEGGD
LVPRLTEPATRTAPSGNTLNLSPSSTPSNPNAPAYVSGDRVTGYSEKGVEMEGHAELRRDGGVIKGDRLTYDQDTDEAHA
TGNVRLSKSGTLAVGPEARMRVQANEGYMLSPDYYFQQTGGSGSAERVDFLDPDRSTLKKATYTTCSPDNADWYFSARKL
DLDSDRQVGTAYGGVLNFFGVPIAGAPAFSFPLNGERRSGVLPPLFGYGSNSGADLTVPYYFNLAPNRDLTIYPRILTSR
GVQLGEDFRYVGDGYSGRIRGEFLPDDKKAGRNRWAYSIQHYQSIIPGMTAYVNVSKVSDDKYPDDLTRSVSQSTLRQYT
QEGGVIYAWQDWVFMARVQKFQTLLPSEPSYEREPQLNAKYNRYDFHGFDISLETDYTRFRIPLTSTGFQQPEGNRAFIQ
PTISYPIIHPGWYVTPKFIFNAAQYNMDAGTNTTGASNTLNRAIPTVSLDSGMTFERDAPGVSKLFGVKYTQTLEPRLFY
VYTPFYDQSQFPLFDTVQSDFGYGQIFTENPFTGNDRIADNNKLTLGLTTRLIESETGVERFRGTIAQRVDFTGQRVQLN
GTLPDAKPSYSDLLAATTIQLFRGYYLDAGIQWNPDQDKVNYSNVALAYRPESRKLINFGYRYRRPTSVTDNTAIDQIEM
SGQWPITQRTYGIGRVAFDKSANQLVDALAGFEYAADCWVGRFVYQRFRNTSNGYTGRVFFQVEFRGLSKIGSNPLDMLR
LNVPGYEPVTARPVPTTPYDHYE

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family

Homologues:

Organism=Escherichia coli, GI1786239, Length=687, Percent_Identity=28.82096069869, Blast_Score=253, Evalue=3e-68,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LPTD_RALME (Q1LRA4)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582591.1
- STRING:   Q1LRA4
- GeneID:   4037224
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0436
- eggNOG:   COG1452
- HOGENOM:   HBG701380
- OMA:   TPYYWNI
- PhylomeDB:   Q1LRA4
- ProtClustDB:   CLSK2302405
- BioCyc:   RMET266264:RMET_0436-MONOMER
- HAMAP:   MF_01411
- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543

Pfam domain/function: PF03968 OstA; PF04453 OstA_C

EC number: NA

Molecular weight: Translated: 91745; Mature: 91614

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQRRSPHHPATRPPAPPGTSRRVRLPASALRPLVLAMAGLTVSAHAQYGATSAIPNID
CCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCC
LVEPVVTQTPEAPPPPAEGGDLVPRLTEPATRTAPSGNTLNLSPSSTPSNPNAPAYVSGD
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEECC
RVTGYSEKGVEMEGHAELRRDGGVIKGDRLTYDQDTDEAHATGNVRLSKSGTLAVGPEAR
EECCCCCCCCCCCCCHHHHHCCCEEECCEEECCCCCCCCCCCCCEEEECCCCEEECCCCE
MRVQANEGYMLSPDYYFQQTGGSGSAERVDFLDPDRSTLKKATYTTCSPDNADWYFSARK
EEEECCCCEEECCCCEEEECCCCCCCCEEECCCCCHHHHHHCEEEECCCCCCCEEEEEEE
LDLDSDRQVGTAYGGVLNFFGVPIAGAPAFSFPLNGERRSGVLPPLFGYGSNSGADLTVP
ECCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEEE
YYFNLAPNRDLTIYPRILTSRGVQLGEDFRYVGDGYSGRIRGEFLPDDKKAGRNRWAYSI
EEEECCCCCCEEEEEHHHHHCCCCCCCCCEEECCCCCCEEECCCCCCHHHCCCCHHEEHH
QHYQSIIPGMTAYVNVSKVSDDKYPDDLTRSVSQSTLRQYTQEGGVIYAWQDWVFMARVQ
HHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
KFQTLLPSEPSYEREPQLNAKYNRYDFHGFDISLETDYTRFRIPLTSTGFQQPEGNRAFI
HHHHHCCCCCCCCCCCCCCCCCCEEEECEEEEEEECCCEEEEEEECCCCCCCCCCCEEEE
QPTISYPIIHPGWYVTPKFIFNAAQYNMDAGTNTTGASNTLNRAIPTVSLDSGMTFERDA
EEECCCCEECCCCEECHHHHEEHHHCCCCCCCCCCCCCCHHHHCCCEEECCCCCEECCCC
PGVSKLFGVKYTQTLEPRLFYVYTPFYDQSQFPLFDTVQSDFGYGQIFTENPFTGNDRIA
CCHHHHHCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCEEC
DNNKLTLGLTTRLIESETGVERFRGTIAQRVDFTGQRVQLNGTLPDAKPSYSDLLAATTI
CCCEEEEEEEEEEHHCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHH
QLFRGYYLDAGIQWNPDQDKVNYSNVALAYRPESRKLINFGYRYRRPTSVTDNTAIDQIE
HHHHCEEEECCEEECCCCCCCCCCCEEEEECCCCCCEEECCEEECCCCCCCCCCCCCEEE
MSGQWPITQRTYGIGRVAFDKSANQLVDALAGFEYAADCWVGRFVYQRFRNTSNGYTGRV
ECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
FFQVEFRGLSKIGSNPLDMLRLNVPGYEPVTARPVPTTPYDHYE
EEEEEECCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEQRRSPHHPATRPPAPPGTSRRVRLPASALRPLVLAMAGLTVSAHAQYGATSAIPNID
CCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCC
LVEPVVTQTPEAPPPPAEGGDLVPRLTEPATRTAPSGNTLNLSPSSTPSNPNAPAYVSGD
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEECC
RVTGYSEKGVEMEGHAELRRDGGVIKGDRLTYDQDTDEAHATGNVRLSKSGTLAVGPEAR
EECCCCCCCCCCCCCHHHHHCCCEEECCEEECCCCCCCCCCCCCEEEECCCCEEECCCCE
MRVQANEGYMLSPDYYFQQTGGSGSAERVDFLDPDRSTLKKATYTTCSPDNADWYFSARK
EEEECCCCEEECCCCEEEECCCCCCCCEEECCCCCHHHHHHCEEEECCCCCCCEEEEEEE
LDLDSDRQVGTAYGGVLNFFGVPIAGAPAFSFPLNGERRSGVLPPLFGYGSNSGADLTVP
ECCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEEE
YYFNLAPNRDLTIYPRILTSRGVQLGEDFRYVGDGYSGRIRGEFLPDDKKAGRNRWAYSI
EEEECCCCCCEEEEEHHHHHCCCCCCCCCEEECCCCCCEEECCCCCCHHHCCCCHHEEHH
QHYQSIIPGMTAYVNVSKVSDDKYPDDLTRSVSQSTLRQYTQEGGVIYAWQDWVFMARVQ
HHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
KFQTLLPSEPSYEREPQLNAKYNRYDFHGFDISLETDYTRFRIPLTSTGFQQPEGNRAFI
HHHHHCCCCCCCCCCCCCCCCCCEEEECEEEEEEECCCEEEEEEECCCCCCCCCCCEEEE
QPTISYPIIHPGWYVTPKFIFNAAQYNMDAGTNTTGASNTLNRAIPTVSLDSGMTFERDA
EEECCCCEECCCCEECHHHHEEHHHCCCCCCCCCCCCCCHHHHCCCEEECCCCCEECCCC
PGVSKLFGVKYTQTLEPRLFYVYTPFYDQSQFPLFDTVQSDFGYGQIFTENPFTGNDRIA
CCHHHHHCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCEEC
DNNKLTLGLTTRLIESETGVERFRGTIAQRVDFTGQRVQLNGTLPDAKPSYSDLLAATTI
CCCEEEEEEEEEEHHCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHH
QLFRGYYLDAGIQWNPDQDKVNYSNVALAYRPESRKLINFGYRYRRPTSVTDNTAIDQIE
HHHHCEEEECCEEECCCCCCCCCCCEEEEECCCCCCEEECCEEECCCCCCCCCCCCCEEE
MSGQWPITQRTYGIGRVAFDKSANQLVDALAGFEYAADCWVGRFVYQRFRNTSNGYTGRV
ECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
FFQVEFRGLSKIGSNPLDMLRLNVPGYEPVTARPVPTTPYDHYE
EEEEEECCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA