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The map label for this gene is dusB [H]

Identifier: 94309374

GI number: 94309374

Start: 453223

End: 454368

Strand: Reverse

Name: dusB [H]

Synonym: Rmet_0429

Alternate gene names: 94309374

Gene position: 454368-453223 (Counterclockwise)

Preceding gene: 94309375

Following gene: 94309373

Centisome position: 11.57

GC content: 65.71

Gene sequence:

>1146_bases
TTGCGCCGGCCCCAAGGCTTGGCAGGGGCATGTTTTTGCATATCGCCAGCTTCGCCTGAACGCACCCAACACCGTTCCAT
CGTGCAGATCGGTCCGCACCAACTTCGCAACAACCTGTTCGTCGCCCCCATGGCCGGGGTGACCGACCGGCCCTTCCGCC
AGCTCTGCAAGCAGCTTGGCGCGGGCTACGCGGTGTCCGAGATGGTTGCGTCGAACGCCCAGCTCTGGAAAAGCGAAAAG
ACCATGCGCCGCGCCAACCACGCCGGCGAGGTGGAACCGATCTCGGTGCAGATCGCAGGCGCGGAGCCGTCGATGATGGC
CGAGGCGGCCCGCTACAACGTGGACCGCGGCGCCCAGATCATCGACATCAACATGGGCTGCCCGGCCAAGAAGGTCTGCA
ACGTGGCCGCCGGTTCGGCGCTGCTGCAGAACGAGCCGCTGGTCGTGCGCATCGTCGAAGCCGTGGTGGCCGCCGTGGGC
GACCGCGTGCCCGTCACGCTGAAGATCCGCACCGGCTGGGACCGCGAGCACAAGAATGCGCTGCGCGTGGCGCGCATGGT
GGAAGACGCCGGCATCAGCATGCTGACGATCCACGGCCGCACGCGCGCCGATCTCTATCATGGCGAAGCCGAGTACGAGA
CGATTGCCGCCGTCAAGTCGTCGATCCGCATCCCCGTGGTAGCCAACGGCGACATCACCACGCCGCAGAAGGCCCGACAT
GTGCTGGCCGTGACCGGCGCCGACGCGATCATGATCGGCCGGGCCGCGCAGGGCCGCCCCTGGCTGTTCCGCGAGATCGA
GCACTACCTGAAGACGGGCGAGCTGCTGCCCTCGCCAGAAGTGGCCGAGATCCGCGCGATCATGAACCATCACCTGGCGG
ACCACTACGCGTTCTACGGCGAGTTCACCGGAGTGCGCACCGCCCGCAAGCATATTGCCTGGTACACGCGCGGCCTCAAG
GGCGCGAACCTGTTCCGGCATCGCATGAACACACTGGAATCCACCGACGCGCAACTCGCGGCCGTCAACGCATTCTTCGA
CGAACAGGCTGCAATTTCCGACCGCCTGGTCTATGTCGACGAGAATGCCGCGCCGGACGACGACGCGCTCGACAACAACA
AGAAACGGGAATTGCTTGCCGCATGA

Upstream 100 bases:

>100_bases
CCGACGGCCCCCTTACGGGGCCGTCTTCATGATATTTGCTCATTTCTTGAGCAACCGTACCGGATTGGCGGTAAAATCCC
GCCCTCGCACATGACCCGAC

Downstream 100 bases:

>100_bases
GCCGCAACGCTATCGACCAATGCATCCGGGACAGCCTGGATACTTACTTTCGCGATCTGGACGGCGAAGTGCCGTCCAAC
ATGTACAACATGGTGCTGGA

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRRPQGLAGACFCISPASPERTQHRSIVQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLGAGYAVSEMVASNAQLWKSEK
TMRRANHAGEVEPISVQIAGAEPSMMAEAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVEAVVAAVG
DRVPVTLKIRTGWDREHKNALRVARMVEDAGISMLTIHGRTRADLYHGEAEYETIAAVKSSIRIPVVANGDITTPQKARH
VLAVTGADAIMIGRAAQGRPWLFREIEHYLKTGELLPSPEVAEIRAIMNHHLADHYAFYGEFTGVRTARKHIAWYTRGLK
GANLFRHRMNTLESTDAQLAAVNAFFDEQAAISDRLVYVDENAAPDDDALDNNKKRELLAA

Sequences:

>Translated_381_residues
MRRPQGLAGACFCISPASPERTQHRSIVQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLGAGYAVSEMVASNAQLWKSEK
TMRRANHAGEVEPISVQIAGAEPSMMAEAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVEAVVAAVG
DRVPVTLKIRTGWDREHKNALRVARMVEDAGISMLTIHGRTRADLYHGEAEYETIAAVKSSIRIPVVANGDITTPQKARH
VLAVTGADAIMIGRAAQGRPWLFREIEHYLKTGELLPSPEVAEIRAIMNHHLADHYAFYGEFTGVRTARKHIAWYTRGLK
GANLFRHRMNTLESTDAQLAAVNAFFDEQAAISDRLVYVDENAAPDDDALDNNKKRELLAA
>Mature_381_residues
MRRPQGLAGACFCISPASPERTQHRSIVQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLGAGYAVSEMVASNAQLWKSEK
TMRRANHAGEVEPISVQIAGAEPSMMAEAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVEAVVAAVG
DRVPVTLKIRTGWDREHKNALRVARMVEDAGISMLTIHGRTRADLYHGEAEYETIAAVKSSIRIPVVANGDITTPQKARH
VLAVTGADAIMIGRAAQGRPWLFREIEHYLKTGELLPSPEVAEIRAIMNHHLADHYAFYGEFTGVRTARKHIAWYTRGLK
GANLFRHRMNTLESTDAQLAAVNAFFDEQAAISDRLVYVDENAAPDDDALDNNKKRELLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=319, Percent_Identity=29.7805642633229, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI239788483, Length=283, Percent_Identity=30.3886925795053, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI40807366, Length=286, Percent_Identity=31.4685314685315, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=31.4159292035398, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI8923374, Length=287, Percent_Identity=27.5261324041812, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789660, Length=323, Percent_Identity=56.9659442724458, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788462, Length=254, Percent_Identity=31.8897637795276, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI145693211, Length=303, Percent_Identity=25.0825082508251, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=288, Percent_Identity=26.3888888888889, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=33.0357142857143, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=244, Percent_Identity=30.7377049180328, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=26.7206477732794, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=323, Percent_Identity=27.8637770897833, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=30.8016877637131, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=28.5087719298246, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=32.0833333333333, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24580595, Length=251, Percent_Identity=31.4741035856574, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920448, Length=251, Percent_Identity=31.4741035856574, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=28.1938325991189, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45549423, Length=288, Percent_Identity=29.5138888888889, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 41840; Mature: 41840

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPQGLAGACFCISPASPERTQHRSIVQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLG
CCCCCCCCEEEEEECCCCCCHHHCCCEEEECHHHHHCCEEEEECCCCCCCHHHHHHHHHC
AGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPISVQIAGAEPSMMAEAARYNVDRGAQI
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEE
IDINMGCPAKKVCNVAAGSALLQNEPLVVRIVEAVVAAVGDRVPVTLKIRTGWDREHKNA
EEEECCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LRVARMVEDAGISMLTIHGRTRADLYHGEAEYETIAAVKSSIRIPVVANGDITTPQKARH
HHHHHHHHHCCCEEEEECCCCCCCEECCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHCE
VLAVTGADAIMIGRAAQGRPWLFREIEHYLKTGELLPSPEVAEIRAIMNHHLADHYAFYG
EEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EFTGVRTARKHIAWYTRGLKGANLFRHRMNTLESTDAQLAAVNAFFDEQAAISDRLVYVD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCEEEEEE
ENAAPDDDALDNNKKRELLAA
CCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MRRPQGLAGACFCISPASPERTQHRSIVQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLG
CCCCCCCCEEEEEECCCCCCHHHCCCEEEECHHHHHCCEEEEECCCCCCCHHHHHHHHHC
AGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPISVQIAGAEPSMMAEAARYNVDRGAQI
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEE
IDINMGCPAKKVCNVAAGSALLQNEPLVVRIVEAVVAAVGDRVPVTLKIRTGWDREHKNA
EEEECCCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LRVARMVEDAGISMLTIHGRTRADLYHGEAEYETIAAVKSSIRIPVVANGDITTPQKARH
HHHHHHHHHCCCEEEEECCCCCCCEECCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHCE
VLAVTGADAIMIGRAAQGRPWLFREIEHYLKTGELLPSPEVAEIRAIMNHHLADHYAFYG
EEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EFTGVRTARKHIAWYTRGLKGANLFRHRMNTLESTDAQLAAVNAFFDEQAAISDRLVYVD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCEEEEEE
ENAAPDDDALDNNKKRELLAA
CCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]