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The map label for this gene is purH [H]

Identifier: 94309372

GI number: 94309372

Start: 451396

End: 452970

Strand: Reverse

Name: purH [H]

Synonym: Rmet_0427

Alternate gene names: 94309372

Gene position: 452970-451396 (Counterclockwise)

Preceding gene: 94309373

Following gene: 94309371

Centisome position: 11.53

GC content: 65.21

Gene sequence:

>1575_bases
ATGATCAAGCAAGCTCTTCTCTCCGTTTCAGACAAAACCGGCATCGTCGACTTCGCGCGCGAACTCAATGCGCTTGGCGT
CACGCTGCTTTCCACCGGCGGTACCGCCAAATTGCTGGCCGAGGCCGGCCTGCCTGTCACGGAGGTGGCCGATTACACGG
GCTTCCCCGAGATGCTCGACGGCCGCGTGAAGACGCTGCACCCGAAGGTTCACGGCGGCATTCTGGCCCGTCGCGACCTG
CCGGAGCACATGGCAGCCCTGGCCGAGCACAACATTCCGACGATCGACCTGCTGGTGGTGAACCTGTACCCGTTCCAGCA
GACCGTGGCCAAGGATGACTGCACGCTGCCGGACGCCATCGAGAACATCGACATCGGCGGCCCGACCATGCTGCGCTCGG
CGGCCAAGAATCACCGCGACGTGACCGTGATCGTCGATCCGGCCGATTACGCGGTGGTACTCGGCGAGATGCGCGCCAAT
GGCAACAGCGTCGGCTACGACACGAACTTCCGGCTGGCTACCAAGGTGTTCGCCCACACCGCCCAATACGACGGCGCAAT
CACGAACTACCTGACCAGCCTCGGCGCCGACAAGTCGCACCAGGCCCGCAGCGCCTACCCGCAGACGCTGAACCTGGCCT
TCGAGAAGGTCCAGGAAATGCGCTACGGCGAAAACCCGCACCAGTCGGCCGCGTTCTACCGCGACCTCAAGGCCTTTGAC
GGCGCGCTGGCCAACTACGTGCAGTTGCAGGGCAAGGAACTGTCCTACAACAACATCGCCGATGCCGACGCCGCGTGGGA
ATGCGTCAAGTCGTTCGACGCCGCCAACAGCGCCGCATGCGTGATCATCAAGCACGCCAATCCGTGCGGCGTGGCGGTTG
GCGCGAACGCGCTGGAAGCCTACGACAAGGCCTTCAAGACCGACTCGACCTCGGCCTTCGGCGGCATCATTGCCTTCAAC
GTCGAACTGGATGAAGCCGCCGCGCAAGCCGTGGCCAAGCAGTTCGTGGAAGTGCTGATCGCCCCGTCGTTCAGCGCGGC
CGCGCGCAACGTGTTCGCCGCCAAGCAGAACGTGCGTCTGCTGGAGATTCCGCTGGGCAAGGGCGTGAATCAGTACGACC
TGAAGCGCGTGGGCGGCGGCCTGCTGGTGCAGGGCCCGGATGCGCGCAACGTGCAGCCGTCCGAACTGCGCGTGGTGACG
CGCCGTCATCCGACGCCGAAGGAAATGGACGATCTGATGTTTGCCTGGCGCGTGGCCAAGTTCGTCAAGTCGAACGCCAT
CGTGTTCTGCGGCAACGGCATGACGCTGGGCGTGGGCGCGGGTCAGATGAGCCGCGTGGACTCGGCACGCATCGCCAGCA
TCAAGGCCCAGAACGCCGGCCTCACGCTGGCGGGTTCGGCCGTGGCATCGGACGCGTTCTTCCCGTTCCGCGATGGTCTG
GACGTGGTGGTCGACGCTGGCGCGACTTGCGTGATCCAGCCGGGCGGCTCGATGCGCGACGATGAAGTGATCGCCGCCGC
CGACGAGCGCGGCATCGCCATGGTGTTGACCGGCACGCGCCACTTCCGCCACTAA

Upstream 100 bases:

>100_bases
CAGTCACTGGCAGCCGCGTATCTCGGCATCAACCGCAACACCCTTCGCAAGAAGCTGCAGCAACACGGTTTACTTTGAAT
TTGACGCGTTCCCCACAGCC

Downstream 100 bases:

>100_bases
AACGGTAGCGAGGGCACATCGCTGCCCTCGCCCCGACGCCTCTCCCGCGCTACGGGAGAGGCGCGCAACCCCCTCCCCTG
CGGCGTAATCCTCTACACTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MIKQALLSVSDKTGIVDFARELNALGVTLLSTGGTAKLLAEAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALAEHNIPTIDLLVVNLYPFQQTVAKDDCTLPDAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLGEMRAN
GNSVGYDTNFRLATKVFAHTAQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAFD
GALANYVQLQGKELSYNNIADADAAWECVKSFDAANSAACVIIKHANPCGVAVGANALEAYDKAFKTDSTSAFGGIIAFN
VELDEAAAQAVAKQFVEVLIAPSFSAAARNVFAAKQNVRLLEIPLGKGVNQYDLKRVGGGLLVQGPDARNVQPSELRVVT
RRHPTPKEMDDLMFAWRVAKFVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
DVVVDAGATCVIQPGGSMRDDEVIAAADERGIAMVLTGTRHFRH

Sequences:

>Translated_524_residues
MIKQALLSVSDKTGIVDFARELNALGVTLLSTGGTAKLLAEAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALAEHNIPTIDLLVVNLYPFQQTVAKDDCTLPDAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLGEMRAN
GNSVGYDTNFRLATKVFAHTAQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAFD
GALANYVQLQGKELSYNNIADADAAWECVKSFDAANSAACVIIKHANPCGVAVGANALEAYDKAFKTDSTSAFGGIIAFN
VELDEAAAQAVAKQFVEVLIAPSFSAAARNVFAAKQNVRLLEIPLGKGVNQYDLKRVGGGLLVQGPDARNVQPSELRVVT
RRHPTPKEMDDLMFAWRVAKFVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
DVVVDAGATCVIQPGGSMRDDEVIAAADERGIAMVLTGTRHFRH
>Mature_524_residues
MIKQALLSVSDKTGIVDFARELNALGVTLLSTGGTAKLLAEAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALAEHNIPTIDLLVVNLYPFQQTVAKDDCTLPDAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLGEMRAN
GNSVGYDTNFRLATKVFAHTAQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAFD
GALANYVQLQGKELSYNNIADADAAWECVKSFDAANSAACVIIKHANPCGVAVGANALEAYDKAFKTDSTSAFGGIIAFN
VELDEAAAQAVAKQFVEVLIAPSFSAAARNVFAAKQNVRLLEIPLGKGVNQYDLKRVGGGLLVQGPDARNVQPSELRVVT
RRHPTPKEMDDLMFAWRVAKFVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
DVVVDAGATCVIQPGGSMRDDEVIAAADERGIAMVLTGTRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=37.8661087866109, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1790439, Length=528, Percent_Identity=62.1212121212121, Blast_Score=644, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=616, Percent_Identity=32.9545454545455, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI71985574, Length=341, Percent_Identity=26.6862170087977, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=33.8461538461538, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323056, Length=487, Percent_Identity=36.3449691991786, Blast_Score=269, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6323768, Length=484, Percent_Identity=35.9504132231405, Blast_Score=265, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24649832, Length=485, Percent_Identity=36.9072164948454, Blast_Score=259, Evalue=4e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55982; Mature: 55982

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQALLSVSDKTGIVDFARELNALGVTLLSTGGTAKLLAEAGLPVTEVADYTGFPEMLD
CCHHHHHHCCCCCCHHHHHHHHHHCEEEEEECCCCHHHHHHCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALAEHNIPTIDLLVVNLYPFQQTVAKDDCTLPDAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEEECHHHHHCCCCCCCCHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLGEMRANGNSVGYDTNFRLATKVFAHT
HCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHH
AQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAFD
HHCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALANYVQLQGKELSYNNIADADAAWECVKSFDAANSAACVIIKHANPCGVAVGANALEA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHH
YDKAFKTDSTSAFGGIIAFNVELDEAAAQAVAKQFVEVLIAPSFSAAARNVFAAKQNVRL
HHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEE
LEIPLGKGVNQYDLKRVGGGLLVQGPDARNVQPSELRVVTRRHPTPKEMDDLMFAWRVAK
EEEECCCCCCHHHHHHCCCCEEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHH
FVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
HHHCCEEEEECCCEEEEECCCCHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCC
DVVVDAGATCVIQPGGSMRDDEVIAAADERGIAMVLTGTRHFRH
EEEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC
>Mature Secondary Structure
MIKQALLSVSDKTGIVDFARELNALGVTLLSTGGTAKLLAEAGLPVTEVADYTGFPEMLD
CCHHHHHHCCCCCCHHHHHHHHHHCEEEEEECCCCHHHHHHCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALAEHNIPTIDLLVVNLYPFQQTVAKDDCTLPDAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEEECHHHHHCCCCCCCCHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPADYAVVLGEMRANGNSVGYDTNFRLATKVFAHT
HCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHH
AQYDGAITNYLTSLGADKSHQARSAYPQTLNLAFEKVQEMRYGENPHQSAAFYRDLKAFD
HHCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALANYVQLQGKELSYNNIADADAAWECVKSFDAANSAACVIIKHANPCGVAVGANALEA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHH
YDKAFKTDSTSAFGGIIAFNVELDEAAAQAVAKQFVEVLIAPSFSAAARNVFAAKQNVRL
HHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEE
LEIPLGKGVNQYDLKRVGGGLLVQGPDARNVQPSELRVVTRRHPTPKEMDDLMFAWRVAK
EEEECCCCCCHHHHHHCCCCEEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHH
FVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL
HHHCCEEEEECCCEEEEECCCCHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCC
DVVVDAGATCVIQPGGSMRDDEVIAAADERGIAMVLTGTRHFRH
EEEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA