Click here to switch to the map view.

The map label for this gene is ruvC [H]

Identifier: 94309371

GI number: 94309371

Start: 450724

End: 451269

Strand: Reverse

Name: ruvC [H]

Synonym: Rmet_0426

Alternate gene names: 94309371

Gene position: 451269-450724 (Counterclockwise)

Preceding gene: 94309372

Following gene: 94309370

Centisome position: 11.49

GC content: 65.38

Gene sequence:

>546_bases
ATGCGTATTCTCGGCATCGACCCCGGTTTGCGAACCACTGGCTTTGGCGTGCTCGAAAAGCACGGCAACAAGCTCGCCTA
TGTCGCGTCGGGCACGATCCGCAGCAATGGCGAGACCAGCCTTCCCGAACGCCTGAAGACGCTCTACGACGGCATCTCCG
AAGTCGCGCGGACCTACGCGCCAGACTGCGCAGCAATTGAAAAGGTCTTCGTCAACGTCAATCCGCAATCGACCCTGTTG
CTCGGCCAGGCACGCGGCGCGGCGATCTGCGGGCTGGTCGGACAAGGGCTGCCTGTATTCGAATACACCGCGCTGCAGCT
CAAGGTGGCCGTGGTCGGATACGGCCGGGCCAACAAGGAGCAGGTTCAGGAAATGGTGATGCGGCTGCTGTCGCTGTCCG
GCCGTCCGAGTTCCGATGCGGCCGATGCGCTTGGCGTGGCGATCTGCCATGCCAATGGCGGCGACACCCTCGGCACGCTG
GCCGGGCTGGCGCCTGAGCTGGCGCGCAAGGGAATGCGCGTGCGGCGCGGCCGGCTGGTCGGCTGA

Upstream 100 bases:

>100_bases
CTCGCCCCGACGCCTCTCCCGCGCTACGGGAGAGGCGCGCAACCCCCTCCCCTGCGGCGTAATCCTCTACACTACGCCCA
TCTCCCCCTGCCCTTGCTTC

Downstream 100 bases:

>100_bases
TTCGTCGCCGGCGAATCGATGCCGATCGATTGCCATTTTCAGAGCAGCGCCTACGGATGCCCGCTACAGCCGGCGTTTAC
AATCGTCGTTCGTGACACCG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MRILGIDPGLRTTGFGVLEKHGNKLAYVASGTIRSNGETSLPERLKTLYDGISEVARTYAPDCAAIEKVFVNVNPQSTLL
LGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKEQVQEMVMRLLSLSGRPSSDAADALGVAICHANGGDTLGTL
AGLAPELARKGMRVRRGRLVG

Sequences:

>Translated_181_residues
MRILGIDPGLRTTGFGVLEKHGNKLAYVASGTIRSNGETSLPERLKTLYDGISEVARTYAPDCAAIEKVFVNVNPQSTLL
LGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKEQVQEMVMRLLSLSGRPSSDAADALGVAICHANGGDTLGTL
AGLAPELARKGMRVRRGRLVG
>Mature_181_residues
MRILGIDPGLRTTGFGVLEKHGNKLAYVASGTIRSNGETSLPERLKTLYDGISEVARTYAPDCAAIEKVFVNVNPQSTLL
LGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKEQVQEMVMRLLSLSGRPSSDAADALGVAICHANGGDTLGTL
AGLAPELARKGMRVRRGRLVG

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=177, Percent_Identity=48.0225988700565, Blast_Score=136, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 19020; Mature: 19020

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILGIDPGLRTTGFGVLEKHGNKLAYVASGTIRSNGETSLPERLKTLYDGISEVARTYA
CEEEECCCCCCCCCCHHHHHCCCEEEEEEECEEECCCCCCHHHHHHHHHHHHHHHHHHCC
PDCAAIEKVFVNVNPQSTLLLGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKE
CCHHHHHHHHEECCCCCEEEEECCCCCEEEHHHCCCCCHHEEHEEEEEEEEEECCCCCHH
QVQEMVMRLLSLSGRPSSDAADALGVAICHANGGDTLGTLAGLAPELARKGMRVRRGRLV
HHHHHHHHHHHCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHCCHHHHHCCHHHHCCCCC
G
C
>Mature Secondary Structure
MRILGIDPGLRTTGFGVLEKHGNKLAYVASGTIRSNGETSLPERLKTLYDGISEVARTYA
CEEEECCCCCCCCCCHHHHHCCCEEEEEEECEEECCCCCCHHHHHHHHHHHHHHHHHHCC
PDCAAIEKVFVNVNPQSTLLLGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKE
CCHHHHHHHHEECCCCCEEEEECCCCCEEEHHHCCCCCHHEEHEEEEEEEEEECCCCCHH
QVQEMVMRLLSLSGRPSSDAADALGVAICHANGGDTLGTLAGLAPELARKGMRVRRGRLV
HHHHHHHHHHHCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHCCHHHHHCCHHHHCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA