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The map label for this gene is tyrS [H]

Identifier: 94309362

GI number: 94309362

Start: 442941

End: 444182

Strand: Direct

Name: tyrS [H]

Synonym: Rmet_0417

Alternate gene names: 94309362

Gene position: 442941-444182 (Clockwise)

Preceding gene: 94309359

Following gene: 94309363

Centisome position: 11.28

GC content: 63.37

Gene sequence:

>1242_bases
ATGAGTGAAGTGTCCCCGGCCGCCGCGGCCAACTATCCACTTACGCCGTCGGTGATGCGCGCACTGGAGATCACCAAGCG
CGGCTGCGACGAGCTCCTGGTTGAATCCGAATGGTTGCAGAAGCTGGCCCGCAGCGAGGCCACTGGCGTGCCGCTGCGGA
TCAAGCTTGGCCTGGACCCGACCGCGCCCGACATCCATCTGGGCCACACCGTGGTCCTGAACAAGCTGCGCCAGCTCCAG
GATCTGGGCCACCAGGTGATCTTCCTGATCGGCGATTTCACGTCGACGATCGGCGATCCCTCCGGCCGCAACTCCACTCG
TCCGCCGCTGACCCGGGAGCAGATCGAGGCCAACGCCCAGACCTACTACCGCCAGGCCAGCCTGGTGCTCGATCCGGCAC
GTACCGAAATCCGCTACAACAGCGAGTGGTGTGACCCATTGGGCGCCCGCGGCATGATCCAGCTCGCGGCCAAGTACACC
GTGGCCCGGATGATGGAGCGCGATGACTTCACCAAACGGTTTCGGTCTGGGATTCCGATTTCGGTCCATGAGTTCCTTTA
TCCGCTCATGCAGGGCTACGATTCCGTGGCGCTGAAGTCGGATCTGGAGCTGGGCGGCACCGACCAGAAGTTCAATCTGC
TGGTGGGCCGCGAGCTGCAGAAGGAGTACGGCCAGGAATCGCAGTGCATCCTGACGATGCCGCTGCTGGTGGGTCTGGAC
GGCGTTGAGAAGATGTCAAAGTCCAAGAACAACTACATCGGCGTGACCGAGGCCCCGAACGACATGTTCGGCAAGCTGAT
GAGCATCTCGGACGAACTGATGTGGCAGTACTACACGCTGCTGTCGTTCCGCCCGATGAGCGAGATCGACCTGATGAAGA
GTGAAGTGGCACTGGGCCGCAACCCGCGCGACTGCAAGGTGGCTCTGGCCCAGGAGATCGTCACGCGTTTCCACAGCCAG
GCCGATGCCGAGAAGGCGCTCGAGGACTTCAATCACCGCGCTCGTGGCGGCGTGCCCGACGACATCCCGGCCGTGAGCCT
CGAAGGCGCGCCGCTGGGCATTGCCCAATTGCTCAAGCAGGCCAATCTCGTGCCGTCGACGTCCGAGGCCAACCGCAATA
TCGAGCAGGGTGGCGTGAAGATCGATGGCGCCGTGGTCAGCGACAAGTCGACCAAGGTGGTCGCCGGCACGTACGTGGTG
CAGGTGGGCAAGCGCCGCTTCGCGCGCGTGACGCTTTCCTGA

Upstream 100 bases:

>100_bases
TTGGCCGACGGTTTATCCCGTCTGGCGCAAAGCGCGGATTATACCAGAACGAATCTGATTGTCCGTCGCAAATTCTTTAT
AGATTTCAAAGACTTACGTC

Downstream 100 bases:

>100_bases
GCCCGGGTCCGCGATGATCGCCCTGATCCAGCGCGTTTCGCAGGCGCGCGTGACCGTGGATGGCCGCACCACCGGCGAGA
TCGGTGCGGGCCTGCTGGCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSEVSPAAAANYPLTPSVMRALEITKRGCDELLVESEWLQKLARSEATGVPLRIKLGLDPTAPDIHLGHTVVLNKLRQLQ
DLGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQTYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYT
VARMMERDDFTKRFRSGIPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQESQCILTMPLLVGLD
GVEKMSKSKNNYIGVTEAPNDMFGKLMSISDELMWQYYTLLSFRPMSEIDLMKSEVALGRNPRDCKVALAQEIVTRFHSQ
ADAEKALEDFNHRARGGVPDDIPAVSLEGAPLGIAQLLKQANLVPSTSEANRNIEQGGVKIDGAVVSDKSTKVVAGTYVV
QVGKRRFARVTLS

Sequences:

>Translated_413_residues
MSEVSPAAAANYPLTPSVMRALEITKRGCDELLVESEWLQKLARSEATGVPLRIKLGLDPTAPDIHLGHTVVLNKLRQLQ
DLGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQTYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYT
VARMMERDDFTKRFRSGIPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQESQCILTMPLLVGLD
GVEKMSKSKNNYIGVTEAPNDMFGKLMSISDELMWQYYTLLSFRPMSEIDLMKSEVALGRNPRDCKVALAQEIVTRFHSQ
ADAEKALEDFNHRARGGVPDDIPAVSLEGAPLGIAQLLKQANLVPSTSEANRNIEQGGVKIDGAVVSDKSTKVVAGTYVV
QVGKRRFARVTLS
>Mature_412_residues
SEVSPAAAANYPLTPSVMRALEITKRGCDELLVESEWLQKLARSEATGVPLRIKLGLDPTAPDIHLGHTVVLNKLRQLQD
LGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQTYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYTV
ARMMERDDFTKRFRSGIPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQESQCILTMPLLVGLDG
VEKMSKSKNNYIGVTEAPNDMFGKLMSISDELMWQYYTLLSFRPMSEIDLMKSEVALGRNPRDCKVALAQEIVTRFHSQA
DAEKALEDFNHRARGGVPDDIPAVSLEGAPLGIAQLLKQANLVPSTSEANRNIEQGGVKIDGAVVSDKSTKVVAGTYVVQ
VGKRRFARVTLS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=407, Percent_Identity=26.2899262899263, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1787925, Length=404, Percent_Identity=27.2277227722772, Blast_Score=121, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=294, Percent_Identity=27.2108843537415, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=371, Percent_Identity=24.7978436657682, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130383, Length=400, Percent_Identity=25, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45701; Mature: 45570

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50006 FHA_DOMAIN ; PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVSPAAAANYPLTPSVMRALEITKRGCDELLVESEWLQKLARSEATGVPLRIKLGLDP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
TAPDIHLGHTVVLNKLRQLQDLGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQ
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHH
TYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYTVARMMERDDFTKRFRSGIPI
HHHHHHHEEECCCHHCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
SVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQESQCILTMPLLVGLD
EHHHHHHHHHHCCCCEEHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCC
GVEKMSKSKNNYIGVTEAPNDMFGKLMSISDELMWQYYTLLSFRPMSEIDLMKSEVALGR
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
NPRDCKVALAQEIVTRFHSQADAEKALEDFNHRARGGVPDDIPAVSLEGAPLGIAQLLKQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
ANLVPSTSEANRNIEQGGVKIDGAVVSDKSTKVVAGTYVVQVGKRRFARVTLS
CCCCCCCCHHCCCHHHCCEEEECEEECCCCCEEEEHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
SEVSPAAAANYPLTPSVMRALEITKRGCDELLVESEWLQKLARSEATGVPLRIKLGLDP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
TAPDIHLGHTVVLNKLRQLQDLGHQVIFLIGDFTSTIGDPSGRNSTRPPLTREQIEANAQ
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHHHHHHHH
TYYRQASLVLDPARTEIRYNSEWCDPLGARGMIQLAAKYTVARMMERDDFTKRFRSGIPI
HHHHHHHEEECCCHHCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
SVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLVGRELQKEYGQESQCILTMPLLVGLD
EHHHHHHHHHHCCCCEEHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCC
GVEKMSKSKNNYIGVTEAPNDMFGKLMSISDELMWQYYTLLSFRPMSEIDLMKSEVALGR
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
NPRDCKVALAQEIVTRFHSQADAEKALEDFNHRARGGVPDDIPAVSLEGAPLGIAQLLKQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
ANLVPSTSEANRNIEQGGVKIDGAVVSDKSTKVVAGTYVVQVGKRRFARVTLS
CCCCCCCCHHCCCHHHCCEEEECEEECCCCCEEEEHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA