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The map label for this gene is gdhA [H]

Identifier: 94309343

GI number: 94309343

Start: 425606

End: 426913

Strand: Direct

Name: gdhA [H]

Synonym: Rmet_0398

Alternate gene names: 94309343

Gene position: 425606-426913 (Clockwise)

Preceding gene: 94309342

Following gene: 94309344

Centisome position: 10.83

GC content: 64.37

Gene sequence:

>1308_bases
ATGTCTTCCGCAGCACCGACGCAAAACGTCTCCAATGGTCATACCCTGCCTTCGTACCTGAACGCCGACAACCTCGGCCC
GTGGGGGATCTACCTGCAGCAAGTCGACCGTGTCACGCCCTACCTGGGCTCGCTGGCACGCTGGGTGGAAACCCTGAAGC
GCCCGAAGCGCGCGATGATCGTCGACGTACCCATCGAACTCGACAACGGCACGATCGCCCACTTCGAGGGCTATCGCGTG
CAGCACAACCTGTCGCGCGGCCCGGGCAAGGGCGGCGTCCGCTTCCACCAGGACGTGACGTTGTCCGAGGTGATGGCGCT
GTCGGCCTGGATGTCGGTCAAGAATGCGGCCGTGAACGTGCCGTACGGCGGCGCCAAGGGCGGTATCCGCGTTGATCCGC
GCACCCTGTCGCACGGCGAACTGGAACGCCTGACCCGCCGGTACACGAGCGAAATCAACATCATCATCGGACCGAGCAAG
GACATTCCCGCCCCGGACGTGAACACCAACGCCCAGGTCATGGCCTGGATGATGGATACCTACTCGATGAACTCGGGCAG
CACGGCCACCGGCGTGGTGACCGGCAAGCCGATCTCGCTGGGCGGTTCGCTGGGTCGTCATGAAGCCACGGGCCGCGGTG
TGTTCGTCGTCGGTTCCGAGGCGGCCCGCAACCTGGGCATGGACGTCAAGGGCGCGCGCGTGGCGGTGCAGGGCTTCGGC
AACGTGGGTGCCGTGGCAGCCAAGCTGTTCCATGAGGCGGGCGCCACGGTGGTGGCGGTGCAGGACCATCGCACCACGCT
GTTCGACCCGGCCGGTCTGGACGTGCCGAAGATGATGGAGTACGCGTCGCACAGCGGCACGATCGAAGGCTACCGCGCCG
AGGTCATCTCCACCGAGCAGTTCTGGGAAGTCGATTGCGACATCCTGATCCCGGCCGCGCTGGAAGGGCAACTGACCGCC
AGCAATGCGCCGAAGATCAAGGCGAAGCTGGTGATCGAGGGCGCCAACGGCCCGACCACGCCCGAGGCCGACGATATTCT
TCGCGAGCGCAATATCCTGGTTTGCCCGGACGTGATCGCCAACGCCGGCGGCGTGACCGTGTCCTACTTTGAATGGGTGC
AGGATTTCTCGAGCTTCTTCTGGACCGAAGAGGAGATCAACGAGCGCCTGGTACGGATCATGCAAGAGGCCTTCCGGGCA
ATCTGGCAGGTTGCCCAGGACAACAAGGTCACGTTGCGGACGGCTGCGTTTATCGTTGCCTGTACGCGTATTCTGCAGGC
GCGCGAAATGCGCGGCCTGTATCCCTGA

Upstream 100 bases:

>100_bases
GTGTCTTGCATAACCCTGCATGGGCGGATAATCAAACGGCATTGGATTCGGGAGTGGGGCCGCCGATATGCTCCGTGTCA
CCTTTACTCGGGACCCAACG

Downstream 100 bases:

>100_bases
TGAGGCAGGGTTCCCGGTGGGCGGCTGGTGCTGCGACGCAGCAAGGTTGCCCATTGCCTGGATGACACCGAAACGCGGCG
ACTGGTTTGTTCCAGTCGCC

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSSAAPTQNVSNGHTLPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMIVDVPIELDNGTIAHFEGYRV
QHNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSHGELERLTRRYTSEINIIIGPSK
DIPAPDVNTNAQVMAWMMDTYSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNLGMDVKGARVAVQGFG
NVGAVAAKLFHEAGATVVAVQDHRTTLFDPAGLDVPKMMEYASHSGTIEGYRAEVISTEQFWEVDCDILIPAALEGQLTA
SNAPKIKAKLVIEGANGPTTPEADDILRERNILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRA
IWQVAQDNKVTLRTAAFIVACTRILQAREMRGLYP

Sequences:

>Translated_435_residues
MSSAAPTQNVSNGHTLPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMIVDVPIELDNGTIAHFEGYRV
QHNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSHGELERLTRRYTSEINIIIGPSK
DIPAPDVNTNAQVMAWMMDTYSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNLGMDVKGARVAVQGFG
NVGAVAAKLFHEAGATVVAVQDHRTTLFDPAGLDVPKMMEYASHSGTIEGYRAEVISTEQFWEVDCDILIPAALEGQLTA
SNAPKIKAKLVIEGANGPTTPEADDILRERNILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRA
IWQVAQDNKVTLRTAAFIVACTRILQAREMRGLYP
>Mature_434_residues
SSAAPTQNVSNGHTLPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMIVDVPIELDNGTIAHFEGYRVQ
HNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSHGELERLTRRYTSEINIIIGPSKD
IPAPDVNTNAQVMAWMMDTYSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNLGMDVKGARVAVQGFGN
VGAVAAKLFHEAGATVVAVQDHRTTLFDPAGLDVPKMMEYASHSGTIEGYRAEVISTEQFWEVDCDILIPAALEGQLTAS
NAPKIKAKLVIEGANGPTTPEADDILRERNILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRAI
WQVAQDNKVTLRTAAFIVACTRILQAREMRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=370, Percent_Identity=44.3243243243243, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI4885281, Length=370, Percent_Identity=43.5135135135135, Blast_Score=293, Evalue=3e-79,
Organism=Escherichia coli, GI1788059, Length=363, Percent_Identity=34.435261707989, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=390, Percent_Identity=38.4615384615385, Blast_Score=263, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6319256, Length=383, Percent_Identity=31.0704960835509, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324951, Length=374, Percent_Identity=30.4812834224599, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24649283, Length=455, Percent_Identity=38.9010989010989, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI45553475, Length=359, Percent_Identity=44.2896935933148, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI45549226, Length=362, Percent_Identity=43.9226519337017, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24649460, Length=312, Percent_Identity=43.9102564102564, Blast_Score=238, Evalue=8e-63,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 47255; Mature: 47124

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAAPTQNVSNGHTLPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMI
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VDVPIELDNGTIAHFEGYRVQHNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNV
EEEEEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEC
PYGGAKGGIRVDPRTLSHGELERLTRRYTSEINIIIGPSKDIPAPDVNTNAQVMAWMMDT
CCCCCCCCEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHEEEEEHHH
YSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNLGMDVKGARVAVQGFG
HCCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCEEEEECCC
NVGAVAAKLFHEAGATVVAVQDHRTTLFDPAGLDVPKMMEYASHSGTIEGYRAEVISTEQ
CHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCH
FWEVDCDILIPAALEGQLTASNAPKIKAKLVIEGANGPTTPEADDILRERNILVCPDVIA
HEECCCCEEECCCCCCEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCEEECCHHHC
NAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRAIWQVAQDNKVTLRTAAFIVA
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
CTRILQAREMRGLYP
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSAAPTQNVSNGHTLPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VDVPIELDNGTIAHFEGYRVQHNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNV
EEEEEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEC
PYGGAKGGIRVDPRTLSHGELERLTRRYTSEINIIIGPSKDIPAPDVNTNAQVMAWMMDT
CCCCCCCCEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHEEEEEHHH
YSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNLGMDVKGARVAVQGFG
HCCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCEEEEECCC
NVGAVAAKLFHEAGATVVAVQDHRTTLFDPAGLDVPKMMEYASHSGTIEGYRAEVISTEQ
CHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCH
FWEVDCDILIPAALEGQLTASNAPKIKAKLVIEGANGPTTPEADDILRERNILVCPDVIA
HEECCCCEEECCCCCCEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCEEECCHHHC
NAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRAIWQVAQDNKVTLRTAAFIVA
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
CTRILQAREMRGLYP
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]