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The map label for this gene is qseD [H]

Identifier: 94309342

GI number: 94309342

Start: 424482

End: 425471

Strand: Direct

Name: qseD [H]

Synonym: Rmet_0397

Alternate gene names: 94309342

Gene position: 424482-425471 (Clockwise)

Preceding gene: 335055488

Following gene: 94309343

Centisome position: 10.81

GC content: 66.87

Gene sequence:

>990_bases
TTGCGCGCCGTGCGTCCGGGCGGGCGCCGGGTGATAATCCGCGCCATGGAAATCAAATGGCTTGAAGACTTCGTCAGCCT
GGCCGAGACGCACAGCTTCTCTCGCTCGGCGGAACTGCGGCATGTCACGCAGCCGGCATTCTCGCGTCGGATCCAGTCGC
TCGAGGCGTGGGTCGGCACCGAACTGATCGACCGTTCGAGTTACCCCACCAGCCTGACTGCCGCCGGCAAGGTGTTCTAT
GAACAGGCGCTGGCGATGCTGGCGCAGGTCAGCGAGACGCGGGCGCTAATGCGCGGCCAGCGCTCGGCCAACGCGCAGGT
GCTGGAATTCGCGGTGCCGCACACGCTGTCGCTGACGTTTTTCCCGGAGTGGCTCAAGGGGGTCGAGCGCAAGGTCGGCA
CGCTGCCATGCCGGCTGCGGGCGCTGAACGTGCACGACGCGGTGCTGATGCTCGTCGAAGGTGGGTGCGACCTCGTCATG
GTCTATCACCACTCGCGCCAGGCCATCCAGCTCGACCCGGCGCACTACGACATGCTGGTGCTCGGCACCGAGCGCCTGTC
TCCCTATAGCGTCCCCGATGCGTCCGGCCGCCCGCTGTTCCGGATGCCCGGCACCGACAAGAAGCCGGTGCCGTACCTGA
GCTACACGCCCAATGCCTTCCTTGGCCGCATGGTGGACATGCTGCTGGCCGAATCGCCGGAAACGCTCAAGCTCGACAAA
TGCTACGAGACTGATATGGCCGAGGCGCTCAAGGTCATGGCGCTGGCCGGGCATGGCATGGCCTTCCTGCCCGAGAGCGC
GGTACGCGAGGACGTTGCCGCCGGCCGGCTGGTGCGCGCCGATGCCGCGAAGGGCTTGCCCCAGTCGATCGACATGGAAA
TCCGGCTCTATCGCGAACGGCCGCCGGAACACGCCAACGAGCGGCGCGGCAGCCAGCAGCGCCGCAAGCGCCAGCTCGTG
GACAAGATCTGGGCGGCGCTGACCTCGTAA

Upstream 100 bases:

>100_bases
ATCATAGGAGGACCCTCCGAGCACAATGATGCAAAATTCAGTCTGGAATGGATTGGAATTGTAATCCTGTTCTACTGGTT
GCGCATTTTTGGGAATGATC

Downstream 100 bases:

>100_bases
AAAACCCATTTCGGGGAGGGGGTAATGATTATGCGTGTCTTGCATAACCCTGCATGGGCGGATAATCAAACGGCATTGGA
TTCGGGAGTGGGGCCGCCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Quorum-sensing regulator protein D [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRAVRPGGRRVIIRAMEIKWLEDFVSLAETHSFSRSAELRHVTQPAFSRRIQSLEAWVGTELIDRSSYPTSLTAAGKVFY
EQALAMLAQVSETRALMRGQRSANAQVLEFAVPHTLSLTFFPEWLKGVERKVGTLPCRLRALNVHDAVLMLVEGGCDLVM
VYHHSRQAIQLDPAHYDMLVLGTERLSPYSVPDASGRPLFRMPGTDKKPVPYLSYTPNAFLGRMVDMLLAESPETLKLDK
CYETDMAEALKVMALAGHGMAFLPESAVREDVAAGRLVRADAAKGLPQSIDMEIRLYRERPPEHANERRGSQQRRKRQLV
DKIWAALTS

Sequences:

>Translated_329_residues
MRAVRPGGRRVIIRAMEIKWLEDFVSLAETHSFSRSAELRHVTQPAFSRRIQSLEAWVGTELIDRSSYPTSLTAAGKVFY
EQALAMLAQVSETRALMRGQRSANAQVLEFAVPHTLSLTFFPEWLKGVERKVGTLPCRLRALNVHDAVLMLVEGGCDLVM
VYHHSRQAIQLDPAHYDMLVLGTERLSPYSVPDASGRPLFRMPGTDKKPVPYLSYTPNAFLGRMVDMLLAESPETLKLDK
CYETDMAEALKVMALAGHGMAFLPESAVREDVAAGRLVRADAAKGLPQSIDMEIRLYRERPPEHANERRGSQQRRKRQLV
DKIWAALTS
>Mature_329_residues
MRAVRPGGRRVIIRAMEIKWLEDFVSLAETHSFSRSAELRHVTQPAFSRRIQSLEAWVGTELIDRSSYPTSLTAAGKVFY
EQALAMLAQVSETRALMRGQRSANAQVLEFAVPHTLSLTFFPEWLKGVERKVGTLPCRLRALNVHDAVLMLVEGGCDLVM
VYHHSRQAIQLDPAHYDMLVLGTERLSPYSVPDASGRPLFRMPGTDKKPVPYLSYTPNAFLGRMVDMLLAESPETLKLDK
CYETDMAEALKVMALAGHGMAFLPESAVREDVAAGRLVRADAAKGLPQSIDMEIRLYRERPPEHANERRGSQQRRKRQLV
DKIWAALTS

Specific function: Down-regulates expression of the flagellar regulon, decreasing motility [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790783, Length=267, Percent_Identity=32.9588014981273, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787879, Length=271, Percent_Identity=25.830258302583, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788887, Length=79, Percent_Identity=40.5063291139241, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36970; Mature: 36970

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVRPGGRRVIIRAMEIKWLEDFVSLAETHSFSRSAELRHVTQPAFSRRIQSLEAWVGT
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELIDRSSYPTSLTAAGKVFYEQALAMLAQVSETRALMRGQRSANAQVLEFAVPHTLSLTF
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEE
FPEWLKGVERKVGTLPCRLRALNVHDAVLMLVEGGCDLVMVYHHSRQAIQLDPAHYDMLV
CHHHHHHHHHHHCCCCCEEEEECHHHEEEEEEECCCCEEEEEECCCCEEEECCCCEEEEE
LGTERLSPYSVPDASGRPLFRMPGTDKKPVPYLSYTPNAFLGRMVDMLLAESPETLKLDK
EECCCCCCCCCCCCCCCEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCEEHHH
CYETDMAEALKVMALAGHGMAFLPESAVREDVAAGRLVRADAAKGLPQSIDMEIRLYRER
HHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHCCEEECHHHCCCCCCCCCEEEEEECC
PPEHANERRGSQQRRKRQLVDKIWAALTS
CCCHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRAVRPGGRRVIIRAMEIKWLEDFVSLAETHSFSRSAELRHVTQPAFSRRIQSLEAWVGT
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELIDRSSYPTSLTAAGKVFYEQALAMLAQVSETRALMRGQRSANAQVLEFAVPHTLSLTF
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEE
FPEWLKGVERKVGTLPCRLRALNVHDAVLMLVEGGCDLVMVYHHSRQAIQLDPAHYDMLV
CHHHHHHHHHHHCCCCCEEEEECHHHEEEEEEECCCCEEEEEECCCCEEEECCCCEEEEE
LGTERLSPYSVPDASGRPLFRMPGTDKKPVPYLSYTPNAFLGRMVDMLLAESPETLKLDK
EECCCCCCCCCCCCCCCEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCEEHHH
CYETDMAEALKVMALAGHGMAFLPESAVREDVAAGRLVRADAAKGLPQSIDMEIRLYRER
HHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHCCEEECHHHCCCCCCCCCEEEEEECC
PPEHANERRGSQQRRKRQLVDKIWAALTS
CCCHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7876157 [H]