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The map label for this gene is ilvA [H]

Identifier: 94309299

GI number: 94309299

Start: 373822

End: 375519

Strand: Reverse

Name: ilvA [H]

Synonym: Rmet_0354

Alternate gene names: 94309299

Gene position: 375519-373822 (Counterclockwise)

Preceding gene: 94309303

Following gene: 94309298

Centisome position: 9.56

GC content: 64.37

Gene sequence:

>1698_bases
GTGTCAGCCGGACGCTGTCCTACAACTGTGGCTGTCACTGCACCCGTCGCACGGCAACTGCCAACCTGGCACTGCCAAGT
GCGCCATCCATTATCATTGGCGGTTTCCGCCGATTTTTCCGGTTTTTTTGCCGCTGCCCCCCGGCGCCGCACTCCGCAGA
CCATGACCCGCACGCCCGCCAAGCCCGACTATCTGAAAAAAATCCTGACCGCCAAGGTCTATGACGTGGCCCAGGAGACC
GAATTGACCTATGCGCCCGCGCTGTCGGCACGCACCGGCAATGCGGTCTGGTTCAAGCGCGAGGACACCCAGCCGGTGTT
CTCGTTCAAGTTGCGGGGGGCGTACAACAAGATGGCGTCCCTCACGCCCGAGGAACTCAAGCGCGGCGTGATCGCTGCTT
CGGCAGGCAACCACGCCCAGGGCGTGGCGCTGGCGGCGGCCCGCATGCAGTGCAAGGCGATCATCGCCATGCCGACCACC
ACGCCGCAGGTGAAGATCGACGCCGTGCGCGAGCGCGGCGGCGAGTGGGTGCAGATCGTTCTGCACGGTGAGTCGTACAG
CGATGCCTACCTGCACGCGGCCGAACTCGAGAAGAAGCACAAGCTCGCGTTCATCCACCCGTTTGACGACCCCGAGGTCA
TCGCCGGCCAGGGCACCATCGCGATGGAGATCCTGCGCCAGCACCCTGGCCCGATCCACGCGATCTTTGTCGCCATCGGC
GGCGGCGGCCTGATCTCCGGCATCGCCGCCTACGTCAAGGCGGTGCGCCCCGAGATCAAGGTGATCGGCGTGCAGACCTT
CGATTCCGATGCCATGAAGCGCTCGGTGGACGCCGGCAAGCGCGTGGAACTGAAGGATGTGGGCCTGTTCTCGGACGGTA
CCGCCGTCAAGCTGGTGGGCAAGGAAACGTTCCGCATCACGCGTGAACTGGTCGACGACATCATCCTGGTCGATACCGAT
GCGATCTGCGCGGGCCTGAAGGATGTGTTCCAGGACACCCGCAGCATCCTCGAGCCGGCCGGCGCGCTGGCCGTGGCCGG
CCTCAAGGCGTACGCCGAGCGCGAGAAGCTCAAGCACCAGCACCTGGTGGCGATCGCCTGCGGCGCCAATATGAACTTCG
ACCGCCTGCGCTTTGTGGCCGAGCGCGCCGAAGTGGGTGAAGCGCGCGAAGCGGTCTTCGCCGTGACGATCCCCGAGGAA
CGCGGCAGCTTCAAGCGCTTCTGCGAACAGGTCGGCACGCGCAGCGTGACCGAGTTCAACTACCGCATCGCCGACAAGGG
CGTGGCGCATATCTTCGTCGGCGTGCAGATCGCCAGCCGCGCGGAGAGCGACAAGATCGCCGCCAACTTCCGCCGCCATG
GCTTTGACACGCTCGACCTGTCGAACGACGAACTGGCCAAGCAGCACATCCGCTACATGGTCGGCGGCCATTCGCCGCTG
GCGCATGACGAACTGCTGTATCGCTTCGAGTTCCCGGAGCGCCCGGGCGCGCTGATGAAGTTCCTGTCCAGCATGAGCCC
GAACTGGAACATCAGCCTGTTCCACTACCGGAACCAGGGCGCGGATACGTCGAACATCCTGGTCGGCATCCAGGTGCCGA
AGAACGAGAAGCGCGCGTTCCGCAGCTTCCTTTCGACGCTCGGTTACGTACACTGGGATGAGACCGACAACCCCGTCTAC
AAGCTGTTCCTTTCCTGA

Upstream 100 bases:

>100_bases
GTCAGCCAGCGTGCCGTCGAAATCCAGAAACAGCGCGGTATCGGGTTCGATGAGAGGTAGCGAAGACATCACGCAAGACC
GTAACACGTCGATTTGGAAG

Downstream 100 bases:

>100_bases
CGCCCCCGATCATGACGCTTCCTGAGCACTCGCCGCTGGGCAAGCCCTCGGCGTACAAGACCGAATACGACGCATCATTG
CTGTTCCCGATCCCGCGCCA

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MSAGRCPTTVAVTAPVARQLPTWHCQVRHPLSLAVSADFSGFFAAAPRRRTPQTMTRTPAKPDYLKKILTAKVYDVAQET
ELTYAPALSARTGNAVWFKREDTQPVFSFKLRGAYNKMASLTPEELKRGVIAASAGNHAQGVALAAARMQCKAIIAMPTT
TPQVKIDAVRERGGEWVQIVLHGESYSDAYLHAAELEKKHKLAFIHPFDDPEVIAGQGTIAMEILRQHPGPIHAIFVAIG
GGGLISGIAAYVKAVRPEIKVIGVQTFDSDAMKRSVDAGKRVELKDVGLFSDGTAVKLVGKETFRITRELVDDIILVDTD
AICAGLKDVFQDTRSILEPAGALAVAGLKAYAEREKLKHQHLVAIACGANMNFDRLRFVAERAEVGEAREAVFAVTIPEE
RGSFKRFCEQVGTRSVTEFNYRIADKGVAHIFVGVQIASRAESDKIAANFRRHGFDTLDLSNDELAKQHIRYMVGGHSPL
AHDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGADTSNILVGIQVPKNEKRAFRSFLSTLGYVHWDETDNPVY
KLFLS

Sequences:

>Translated_565_residues
MSAGRCPTTVAVTAPVARQLPTWHCQVRHPLSLAVSADFSGFFAAAPRRRTPQTMTRTPAKPDYLKKILTAKVYDVAQET
ELTYAPALSARTGNAVWFKREDTQPVFSFKLRGAYNKMASLTPEELKRGVIAASAGNHAQGVALAAARMQCKAIIAMPTT
TPQVKIDAVRERGGEWVQIVLHGESYSDAYLHAAELEKKHKLAFIHPFDDPEVIAGQGTIAMEILRQHPGPIHAIFVAIG
GGGLISGIAAYVKAVRPEIKVIGVQTFDSDAMKRSVDAGKRVELKDVGLFSDGTAVKLVGKETFRITRELVDDIILVDTD
AICAGLKDVFQDTRSILEPAGALAVAGLKAYAEREKLKHQHLVAIACGANMNFDRLRFVAERAEVGEAREAVFAVTIPEE
RGSFKRFCEQVGTRSVTEFNYRIADKGVAHIFVGVQIASRAESDKIAANFRRHGFDTLDLSNDELAKQHIRYMVGGHSPL
AHDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGADTSNILVGIQVPKNEKRAFRSFLSTLGYVHWDETDNPVY
KLFLS
>Mature_564_residues
SAGRCPTTVAVTAPVARQLPTWHCQVRHPLSLAVSADFSGFFAAAPRRRTPQTMTRTPAKPDYLKKILTAKVYDVAQETE
LTYAPALSARTGNAVWFKREDTQPVFSFKLRGAYNKMASLTPEELKRGVIAASAGNHAQGVALAAARMQCKAIIAMPTTT
PQVKIDAVRERGGEWVQIVLHGESYSDAYLHAAELEKKHKLAFIHPFDDPEVIAGQGTIAMEILRQHPGPIHAIFVAIGG
GGLISGIAAYVKAVRPEIKVIGVQTFDSDAMKRSVDAGKRVELKDVGLFSDGTAVKLVGKETFRITRELVDDIILVDTDA
ICAGLKDVFQDTRSILEPAGALAVAGLKAYAEREKLKHQHLVAIACGANMNFDRLRFVAERAEVGEAREAVFAVTIPEER
GSFKRFCEQVGTRSVTEFNYRIADKGVAHIFVGVQIASRAESDKIAANFRRHGFDTLDLSNDELAKQHIRYMVGGHSPLA
HDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGADTSNILVGIQVPKNEKRAFRSFLSTLGYVHWDETDNPVYK
LFLS

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=307, Percent_Identity=32.2475570032573, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI33469958, Length=313, Percent_Identity=27.7955271565495, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI19923959, Length=325, Percent_Identity=25.8461538461538, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1790207, Length=514, Percent_Identity=50.1945525291829, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1789505, Length=311, Percent_Identity=37.620578778135, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71991565, Length=357, Percent_Identity=29.1316526610644, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17508781, Length=284, Percent_Identity=31.6901408450704, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17537387, Length=336, Percent_Identity=30.3571428571429, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320930, Length=523, Percent_Identity=47.6099426386233, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322631, Length=306, Percent_Identity=33.0065359477124, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319788, Length=269, Percent_Identity=26.7657992565056, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21355833, Length=316, Percent_Identity=29.1139240506329, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645328, Length=252, Percent_Identity=27.3809523809524, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 62318; Mature: 62187

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGRCPTTVAVTAPVARQLPTWHCQVRHPLSLAVSADFSGFFAAAPRRRTPQTMTRTPA
CCCCCCCCEEEEECHHHHCCCCCEEEECCCEEEEEECCCCCCEECCCCCCCCCHHHCCCC
KPDYLKKILTAKVYDVAQETELTYAPALSARTGNAVWFKREDTQPVFSFKLRGAYNKMAS
CHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCCEEEEEECCHHHHHHC
LTPEELKRGVIAASAGNHAQGVALAAARMQCKAIIAMPTTTPQVKIDAVRERGGEWVQIV
CCHHHHHCCEEEECCCCCCCHHHHHHHHHEEEEEEEECCCCCCEEEHHHHHCCCCEEEEE
LHGESYSDAYLHAAELEKKHKLAFIHPFDDPEVIAGQGTIAMEILRQHPGPIHAIFVAIG
EECCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEECCCHHHHHHHHHCCCCEEEEEEEEC
GGGLISGIAAYVKAVRPEIKVIGVQTFDSDAMKRSVDAGKRVELKDVGLFSDGTAVKLVG
CCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHCCCCCEEEEEECCEECCCCEEEEEC
KETFRITRELVDDIILVDTDAICAGLKDVFQDTRSILEPAGALAVAGLKAYAEREKLKHQ
HHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
HLVAIACGANMNFDRLRFVAERAEVGEAREAVFAVTIPEERGSFKRFCEQVGTRSVTEFN
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEE
YRIADKGVAHIFVGVQIASRAESDKIAANFRRHGFDTLDLSNDELAKQHIRYMVGGHSPL
EEECCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCC
AHDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGADTSNILVGIQVPKNEKRAF
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHH
RSFLSTLGYVHWDETDNPVYKLFLS
HHHHHHHCEEEECCCCCCEEEEEEC
>Mature Secondary Structure 
SAGRCPTTVAVTAPVARQLPTWHCQVRHPLSLAVSADFSGFFAAAPRRRTPQTMTRTPA
CCCCCCCEEEEECHHHHCCCCCEEEECCCEEEEEECCCCCCEECCCCCCCCCHHHCCCC
KPDYLKKILTAKVYDVAQETELTYAPALSARTGNAVWFKREDTQPVFSFKLRGAYNKMAS
CHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCCEEEEEECCHHHHHHC
LTPEELKRGVIAASAGNHAQGVALAAARMQCKAIIAMPTTTPQVKIDAVRERGGEWVQIV
CCHHHHHCCEEEECCCCCCCHHHHHHHHHEEEEEEEECCCCCCEEEHHHHHCCCCEEEEE
LHGESYSDAYLHAAELEKKHKLAFIHPFDDPEVIAGQGTIAMEILRQHPGPIHAIFVAIG
EECCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEECCCHHHHHHHHHCCCCEEEEEEEEC
GGGLISGIAAYVKAVRPEIKVIGVQTFDSDAMKRSVDAGKRVELKDVGLFSDGTAVKLVG
CCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHCCCCCEEEEEECCEECCCCEEEEEC
KETFRITRELVDDIILVDTDAICAGLKDVFQDTRSILEPAGALAVAGLKAYAEREKLKHQ
HHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
HLVAIACGANMNFDRLRFVAERAEVGEAREAVFAVTIPEERGSFKRFCEQVGTRSVTEFN
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEE
YRIADKGVAHIFVGVQIASRAESDKIAANFRRHGFDTLDLSNDELAKQHIRYMVGGHSPL
EEECCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCC
AHDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGADTSNILVGIQVPKNEKRAF
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHH
RSFLSTLGYVHWDETDNPVYKLFLS
HHHHHHHCEEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA